iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns 1708891 from Bio-Rad

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ItemiScript™ cDNA Synthesis Kit, 100 x 20 µl rxns
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1708891
Brand/TrademarkLSG GXD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

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(1) A systemic analysis of monocarboxylate transporters in ovarian cancer and possible therapeutic interventionsChannels (Austin)December 31, 2023Priti Chatterjee, Debaleena Bhowmik, Sib Sankar Roysubsequent phase separation with Chloroform and precipitation with isopropanol. iScript (BioRad, Cat #1708891) cDNA synthesis kit was utilized to prepare cDNA from total RNA. Real-time PCR was performed in Applied

(2) miR-146a inhibits ovarian tumor growth in vivo via targeting immunosuppressive neutrophils and enhancing CD8+ T cell infiltrationMolecular Therapy - OncolyticsDecember 19, 2023Rui Chen, Elaina Coleborn, Chintan Bhavsar, Yue Wang, Louisa Alim, Andrew N. Wilkinson, Michelle A. Tran, Gowri Irgam, Sharat Atluri, Kiefer Wong, et al.IRAK1 mRNA, 1 μg of RNA was reverse-transcribed using an iScript cDNA synthesis kit (Bio-Rad, cat. no. 1708891) according to manufacturer’s protocol. The relative mRNA expression was quantified with qPCR using SYBR

(3) Distinct hypoxia-induced translational profiles of embryonic and adult-derived macrophagesiScienceDecember 15, 2023Nicholas S. Wilcox, Timur O. Yarovinsky, Prakruti Pandya, Vinod S. Ramgolam, Albertomaria Moro, Yinyu Wu, Stefania Nicoli, Karen K. Hirschi, Jeffrey R. Benderassays ,, Direct-Zol RNA MiniPrep Plus,Zymo Research,Cat # R2073 iScript cDNA Synthesis Kit,BioRad,Cat # 1708891 Zenon™ Alexa Fluor™ Mouse IgG1 Labeling Kit,ThermoFisher,"Cat # Z25008, RRID: AB_2736959" ELISA MAX™

(4) Single nucleotide polymorphisms rs148582811 regulates its host gene ARVCF expression to affect nicotine-associated hippocampus-dependent memoryiScienceDecember 15, 2023Zhongli Yang, Jiali Chen, Haijun Han, Yan Wang, Xiaoqiang Shi, Bin Zhang, Ying Mao, Andria N. Li, Wenji Yuan, Jianhua Yao, et al.Kit,QIAGEN,51304 NEBNext® Ultra™ DNA Library Prep kit,Illumina,E7103-E7645 iScript cDNA Synthesis kit,Bio-Rad,1708891 Dual-Lucifearse Reporter Assay,Promega,E1910 BCA protein assay,Beyotime,P0010 NE-PER™ Nuclear and

(5) Alternative oxidase promotes high iron tolerance in Candida albicansMicrobiology SpectrumDecember 12, 2023Rishabh Sharma, Andrew A. Gibb, Kelcie Barnts, John W. Elrod, Sumant PuriDNA-free RNA sample was used for complementary DNA (cDNA) synthesis using iScript cDNA synthesis kit (1708891, Bio-Rad). Furthermore, equal volume (1 µL) of cDNA was used for determining transcript levels via reverse

(6) Transcriptome analysis reveals molecular signature and cell-type difference of Homo sapiens endothelial-to-mesenchymal transitionG3: Genes, Genomes, GeneticsDecember 6, 2023Ronald Bronson, Junfang Lyu, Jianhua Xiongdigestion (Zymo Research). Complementary DNA (cDNA) was prepared using the iScript cDNA Synthesis Kit (1708891, Bio-Rad Laboratories). Real-time qPCR was performed on a CFX Connect Real-Time System (Bio-Rad Laboratories)

(7) Signal regulatory protein beta 2 is a novel positive regulator of innate anticancer immunityFront ImmunolDecember 5, 2023Nienke Visser, Levi Collin Nelemans, Yuan He, Harm Jan Lourens, Macarena González Corrales, Gerwin Huls, Valerie R. Wiersma, Jan Jacob Schuringa, Edwin Bremerfollowing the manufacturers protocol. cDNA was synthesized using an iScript™ cDNA Synthesis Kit (Biorad; #1708891), following manufacturer protocol for 1000 ng/20 μL. 5 μL SYBRgreen (Biorad, #1725274) was added to

(8) Microinjection of β-glucan Into Larval Zebrafish (Danio rerio) for the Assessment of a Trained-Like Immunity PhenotypeBio ProtocDecember 5, 2023Hannah Darroch, Jonathan W. Astin, Christopher J. Hall(PTU) (Sigma-Aldrich, catalog number: P7629)iScriptTM cDNA Synthesis kit (Bio-Rad, catalog number: 1708891)PerfeCTa SYBR Green FastMix with ROX (Quanta Biosciences, catalog number: 95055-500)1 M Tris (pH 9)

(9) A ketogenic diet reduces age-induced chronic neuroinflammation in mice Running title: ketogenic diet and brain inflammagingbioRxivDecember 4, 2023Mitsunori Nomura, Natalia Faraj Murad, Sidharth S Madhavan, Brenda Eap, Thelma Y Garcia, Carlos Galicia Aguirre, Eric Verdin, Lisa Ellerby, David Furman, John C NewmanKit (R1051; Zymo Research) and cDNA synthesis was carried out with Superscript cDNA Synthesis Kit (1708891; BioRad) and real-time quantitative PCR was performed using iTaq Universal SYBR Green Supermix (1725121;

(10) Temporal expression and spatial distribution of the proteoglycan versican during cardiac fibrosis developmentMatrix Biology PlusDecember 1, 2023Athiramol Sasi, Andreas Romaine, Pugazendhi Murugan Erusappan, Arne Olav Melleby, Almira Hasic, Christen Peder Dahl, Kaspar Broch, Vibeke Marie Almaas, Rosa Doñate Puertas, H. Llewelyn Roderick, et al.respectively. cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, CA, #1708891). The primers used for human and mouse versican and for the isoforms V0, V1, V2, V3 have been published

(11) PDGFRβ Activation Induced the Bovine Embryonic Genome Activation via Enhanced NFYA Nuclear LocalizationInt J Mol SciDecember 1, 2023Chalani Dilshani Perera, Muhammad Idrees, Abdul Majid Khan, Zaheer Haider, Safeer Ullah, Ji-Su Kang, Seo-Hyun Lee, Seon-Min Kang, Il-Keun Kongreverse transcription using an iScript cDNA synthesis kit (Bio-Rad Laboratories, Hercules, CA, USA; cat. #1708891) in a total volume of 20 µL according to the manufacturer’s guidelines. As explained previously, real-time

(12) Characterization of erythroferrone structural domains relevant to its iron-regulatory functionJournal of Biological ChemistryDecember 1, 2023Daniel N. Srole, Grace Jung, Alan J. Waring, Elizabeta Nemeth, Tomas GanzFive hundred nanograms of RNA was reverse-transcribed using the iScript cDNA Synthesis Kit (Bio-Rad #1708891). Quantitative real-time PCR was performed on cDNA using SsoAdvanced SYBR Green Supermix (Bio-Rad #

(13) ATM Mutations Associate with Distinct Co-Mutational Patterns and Therapeutic Vulnerabilities in NSCLCClinical Cancer ResearchDecember 1, 2023Natalie I. Vokes, Ana Galan Cobo, Margarita Fernandez-Chas, David Molkentine, Santiago Treviño, Vitaly Druker, Yu Qian, Sonia Patel, Stephanie Schmidt, Lingzhi Hong, et al.260/280 ratios greater than 2.0. 1 μg total RNA was reverse transcribed using iScript reagent (Bio-Rad, 1708891). Gene expression was quantified using TaqMan Fast Advance Master Mix (Applied Biosystems, 4444557,

(14) Evaluation of the SH-SY5Y cell line as an in vitro model for potency testing of a neuropeptide-expressing AAV vectorFrontiers in Molecular NeuroscienceNovember 30, 2023Jeanette Zanker, Daniela Hüser, Adrien Savy, Sara Lázaro-Petri, Eva-Maria Hammer, Christoph Schwarzer, Regine Heilbronnand heating to 65°C for 15 min. cDNA was generated using the iScript™ cDNA Synthesis Kit (Bio-Rad—no. 1708891) following the manufacturer's protocol. Nucleic acid expression profiles were quantified using the QX200

(15) A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP DifferentiationDentistry JournalNovember 27, 2023Cristine Smoczer, Yun K. Park, James B. Herrington, Mazin A. Askar, Sarah Plecha, Eric Krukonis, Susan B. PaurazasUSA). The first-strand cDNA was synthesized from 200 ng of RNA using the iScript cDNA synthesis kit (#1708891, BioRad, Hercules, CA, USA). PCR amplification for DSPP1, BSP1, OCN, and RUNX2 was performed on a BioRad

(16) Inhibiting the endoplasmic reticulum stress response enhances the effect of doxorubicin by altering the lipid metabolism of liver cancer cellsMolecular MetabolismNovember 27, 2023Maria Kopsida, Ada Lerma Clavero, Jaafar Khaled, David Balgoma, Clara Luna-Marco, Azazul Chowdhury, Sofi Sennefelt Nyman, Fredrik Rorsman, Charlotte Ebeling Ebeling Barbier, Peter Bergsten, et al.guidelines. Quality and quantity of RNA was evaluated using Nanodrop. The iScript cDNA-synthesis kit (1708891, Bio-rad, Solna, Sweden) was used according to the manufacturer's protocol. Primers were designed using

(17) An engineered glioblastoma model yields novel macrophage-secreted drivers of invasionbioRxivNovember 23, 2023Erin A. Akins, Dana Wilkins, Manish K. Aghi, Sanjay KumarNanodrop. cDNA synthesis cDNA was synthesized from extracted RNA using iScript cDNA Synthesis Kit (BioRad, #1708891) following the manufacturer’s protocol. RNA-sequencing and differential gene expression analysis Isolated

(18) P2X7 receptor activation leads to NLRP3-independent IL-1β release by human macrophagesCell Communication and SignalingNovember 23, 2023Judith Bockstiegel, Jonas Engelhardt, Günther Weindlthe manufacturer’s protocol. Hence, cDNA was synthesized with the help of iScript cDNA Synthesis Kit (1708891, Bio-Rad, Feldkirchen, Germany). Quantitative real-time RT-PCR (qRT-PCR) was performed as previously

(19) Electroacupuncture modulates glutamate neurotransmission to alleviate PTSD-like behaviors in a PTSD animal modelTranslational PsychiatryNovember 22, 2023Mudan Cai, Hee Ra Park, Eun Jin YangSeongnam-Si, Korea) according to the manufacturer’s protocol. cDNA was synthesized using a cDNA synthesis kit (1708891; Bio-Rad) according to the manufacturer’s instructions. After cDNA synthesis, each sample template was

(20) Hepatocyte integrity depends on c-Jun-controlled proliferation in Schistosoma mansoni infected miceScientific ReportsNovember 21, 2023Lukas Härle, Verena von Bülow, Lukas Knedla, Frederik Stettler, Heike Müller, Daniel Zahner, Simone Haeberlein, Anita Windhorst, Annette Tschuschner, Monika Burg-Roderfeld, et al.protocol, and cDNA synthesis was performed with iScript cDNA Synthesis Kit from Bio-Rad (Cat. No. #1708891) (Bio-Rad Laboratories, Inc., Hercules, California, USA) according to manufacturer’s protocol. RT-qPCR

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(1)

6/10/2022Great Kit for cDNA Synthesis Francesca ParadisoWe utilized this kit with different RNA templates from cell lines and primary cells and with a range of 100ng to 1ug RNA templates. Great reproducibility and efficiency with low RNA template.
(1)

12/18/2018Fast cDNA Synthesis Kit from Bio-Rad F. BiotaNowadays most researchers are begging to study the transcriptome by evaluating individual gene expression using qPCR platforms. Importantly, making cDNA using various RT-PCR reagents has been the ... Read Review
(1)

5/24/2017Super Quick cDNA Julia JacksonWe use this kit in conjunction with Bio-Rad's sso sybr green mix and the CFX connect. Standard qPCR from RNA isolated from cultured or sorted cells, or powdered tissue.
(1)

12/15/2016Good And Simple Kit For Making cDNA Rebecca GWe extract RNA from large numbers of mouse retina samples and need to use reverse-transcription PCR to make cDNA from this RNA. The cDNA is then run on qPCR to detect the expression of our target ... Read Review
(1)

9/2/2016A One Step cDNA Synthesis Kit sndp kmrReal time PCR requires high-quality cDNA for quantification of gene expression. iScript cDNA synthesis kit is very useful in converting RNA to cDNA. This simple kit supplies you with 5X master mix and... Read Review
(1)

6/28/2016cDNA synthesis kit Gulshan SinghKit is used to make cDNA from RNA.

iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns

iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns from Bio-Rad

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iScript™ cDNA Synthesis Kit, 100 x 20 µl rxns from Bio-Rad

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