cDNA Synthesis

cDNA synthesis, also known as reverse transcription, generates complementary DNA (cDNA) from an RNA template. Unsatisfactory cDNA yields can arise from low RNA concentration, target gene complexity, and reagent inconsistencies. Various kits and reagents can help address these issues. cDNA synthesis kits are inclusive of necessary reaction components, including polymerase, primers, buffers, and dNTPs, along with optimized protocols for streamlined sample preparation. Custom primers can be used in place of the kit-supplied random primers or oligo(dT), allowing researchers to manipulate cDNA strand synthesis for maximum yield and accuracy. For RNA starting material, RNA extraction kits are available, designed to produce high-quality RNA yields from a variety of cell and tissue sources. Other reagents such as RNase inhibitors and nuclease-free water can ensure that that the starting samples and the end products are protected from degradation. Browse through our directory of cDNA synthesis products and find solutions for applications ranging from library construction to quantitative reverse transcription PCR (RT-qPCR).