
RNA, or ribonucleic acid, is not only a predecessor of protein formation, but also a regulator of gene expression. Total RNA, mRNA, or small RNA is very difficult to isolate due to its instability and biologically low quantities. It can be challenging to accumulate an adequate amount of rare sample tissue or cells necessary to carry out a series of experiments. Purchasing purified RNA is simplified with the multitude of suppliers that offer several forms of RNA from multifarious organisms, tissues, and cells. RNA samples are prepared in reagents that are compatible with subsequent isolation protocols for library construction or assays such as quantitative real-time PCR. The analysis of gene expression suppression is feasible using miRNA (microRNA) from adult and fetal tissue sources, and from mouse brain. Obtaining yeast tRNA or feline total RNA from bone marrow in practicable concentrations suitable for diverse applications is quick and simple.
Technical advances are helping surmount obstacles.
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A rundown of methods organized by throughput
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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The Sapphire FL Biomolecular Imager is a field-upgradable laser scanner, with a wide selection of user-adjustable lasers and filters. It features 5-micron resolution, adjustable Z-plane focus and a wide field of view of 25cm X 25cm. Applications ...
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We use this kit for the manual RNAscope assay to measure the expression of mRNAs of interest (e.g., Oprm1, the gene encoding the µ-opioid receptor, and Cnr1, the gene encoding cannabinoid receptor 1) in mouse brain tissue. We combine the RNAscope ...
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I used the Geneaid GENEzol TriRNA Pure Kit to extract total RNA from both cell culture and tissue, and it has become my go-to method. The kit cleverly combines the familiar phenol/guanidine isothiocyanate lysis chemistry with a spin-column workflow, ...
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