
RNA, or ribonucleic acid, is not only a predecessor of protein formation, but also a regulator of gene expression. Total RNA, mRNA, or small RNA is very difficult to isolate due to its instability and biologically low quantities. It can be challenging to accumulate an adequate amount of rare sample tissue or cells necessary to carry out a series of experiments. Purchasing purified RNA is simplified with the multitude of suppliers that offer several forms of RNA from multifarious organisms, tissues, and cells. RNA samples are prepared in reagents that are compatible with subsequent isolation protocols for library construction or assays such as quantitative real-time PCR. The analysis of gene expression suppression is feasible using miRNA (microRNA) from adult and fetal tissue sources, and from mouse brain. Obtaining yeast tRNA or feline total RNA from bone marrow in practicable concentrations suitable for diverse applications is quick and simple.
Technique is helping accelerate the drug discovery pipeline.
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PCR, AI, and NGS have all made significant contributions
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Choose the CFX Opus real-time PCR system for the reliability you depend on from Bio-Rad with improved connectivity and performance for your modern lab. The CFX Opus system is the next evolution in qPCR from Bio-Rad. With improved thermal performance ...
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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