
RNA, or ribonucleic acid, is not only a predecessor of protein formation, but also a regulator of gene expression. Total RNA, mRNA, or small RNA is very difficult to isolate due to its instability and biologically low quantities. It can be challenging to accumulate an adequate amount of rare sample tissue or cells necessary to carry out a series of experiments. Purchasing purified RNA is simplified with the multitude of suppliers that offer several forms of RNA from multifarious organisms, tissues, and cells. RNA samples are prepared in reagents that are compatible with subsequent isolation protocols for library construction or assays such as quantitative real-time PCR. The analysis of gene expression suppression is feasible using miRNA (microRNA) from adult and fetal tissue sources, and from mouse brain. Obtaining yeast tRNA or feline total RNA from bone marrow in practicable concentrations suitable for diverse applications is quick and simple.
Industry insiders share what’s on their radar for next year.
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Advice for preparing high-quality libraries from FFPE samples
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The MilliSentials™ Lab Labeling System provides a complete laboratory labeling solution with laboratory grade labels, a compact WIFI capable printer, and custom-designed laboratory labeling software. Features:
Laboratory grade labels
Labels can ...
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Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
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I study gene signaling pathways and need high-quality RNA as input for RT-qPCR and transcript profiling. I use this kit to purify total RNA directly from cells lysed in TRIzol Reagent, skipping the phase separation and precipitation steps of ...
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I used Thermo Scientific RNase A to degrade unprotected mRNA during a gel retention assay to evaluate the protective effect of lipid nanoparticles (LNPs). Using 1 µL RNase A provided efficient RNA digestion while allowing clear discrimination between...
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