RNA, or ribonucleic acid, is not only a predecessor of protein formation, but also a regulator of gene expression. Total RNA, mRNA, or small RNA is very difficult to isolate due to its instability and biologically low quantities. It can be challenging to accumulate an adequate amount of rare sample tissue or cells necessary to carry out a series of experiments. Purchasing purified RNA is simplified with the multitude of suppliers that offer several forms of RNA from multifarious organisms, tissues, and cells. RNA samples are prepared in reagents that are compatible with subsequent isolation protocols for library construction or assays such as quantitative real-time PCR. The analysis of gene expression suppression is feasible using miRNA (microRNA) from adult and fetal tissue sources, and from mouse brain. Obtaining yeast tRNA or feline total RNA from bone marrow in practicable concentrations suitable for diverse applications is quick and simple.
Technical advances are helping surmount obstacles.
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A pipeline of possibilities
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A new version of total RNA extraction ‘miniprep’ kit, featuring upgraded spin columns precision-engineered for high performance
Purify up to 100 µg of high-quality total RNA from multiple sample types including tough-to-lyse samples with this ...
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Speed and enhanced PCR optimization functions like the 2D-Gradient make the Mastercycler X50 the ideal PCR cycler for advanced research in molecular biology. The excellent block temperature regulation gives rise to the next stage of PCR ...
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We study astrocyte bioenergetics and immunometabolism using mouse models. Recently, we found that mice lacking mu opioid receptors in astrocytes exhibit increased mitochondrial metabolism, as well as increased astrocyte reactivity.
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In our experiments, we studied how the transcription factor NPAS3 regulates astrocyte mitochondrial bioenergetics and how this affects the behavioral phenotype of Npas3-deficient mice, with a focus on cognition. We used RNA ISH using the “BaseScope” ...
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