ExPERT ATx® Electroporation System from MaxCyte

Product Specs
ItemExPERT ATx® Electroporation System
CompanyMaxCyte
Price Get Pricing Get Quote for this Product
Catalog NumberE-ATx
QuantityEA
MethodStatic Electroporation
Dimensions(WxDxH) 8 x 17.2 x 19.4 in. (203 x 437 x 493 mm)
ApplicationsCell Therapy, Gene Editing, Antibody & Protein Production, Cell Based Assays
Throughput1x10⁵-7x10⁸ cells per sample
Weight48 lbs (21.8 Kg)
Volume15 µL to 3.5 mL
Volume Range20 µL to 3.5 mL

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MaxCyte

9713 Key West Avenue

Suite 400

Rockville, MD 20850

United States

Phone: +1-301-944-1700

Company Profile

Website: maxcyte.com

(1) Base Editing of TIGIT Reprograms CD155 Signaling in Natural Killer Cells to Enhance Cancer Immunotherapy EfficacyCancer ResApril 15, 2026Cheng Fang, Guanglei Li, Min Han, Yiming Wang, Wenxia Yu, Dan Hu, Jiaojiao Luo, Lixin Wang, Wenwen Fan, Liling Wang, et al.augmented gene editing efficiency (Supplementary Fig. S1C). We also assessed the potential of MaxCyte ExPERT ATx, an alternative nucleofection instrument, to achieve efficient gene editing in PB-NK cells. As expected,

(2) Transient mRNA-based CD117 CAR T cells effectively target acute myeloid leukemia in vitro for potential use as a preconditioning strategyImmuno-Oncology and TechnologyMarch 9, 2026T. Inthanachai, C. Sittplangkoon, H. Tashiro, S. Yagyu, T. Palaga, S. Tawinwung, K. Suppipattransferred into ExPERT™ Processing Assemblies OC-25 and electroporated using the Expanded T-3 program on the ExPERT ATx™ system (MaxCyte, Germany). Following electroporation, cells were transferred into 6-well plates containing

(3) Establishment of a novel human amniotic epithelial-derived cell line, HAT, for high-yield AAV vector productionMolecular Therapy Methods & Clinical DevelopmentDecember 11, 2025Yugo Hirai, Yu-Hsin Chang, Arisa Yamamoto, Ryo Asahina, Rena Moromizato, Mawo Kinoshita, Kazuko Aizawa, Manami Miyai, Michi Kubota, Takayuki Horiuchi, et al.LTX (Lipo-LTX) with PLUS reagent (Invitrogen, Carlsbad, CA), Lipofectamine 2000 CD (Invitrogen). and ExPERT ATx (MaxCyte, Rockville, MD) according to the manufacturers’ instructions. At 6 h post-transfection, the

(4) RNA helicase DDX3X promotes NK cell survival by supporting MCL1 expressionThe Journal of ImmunologyDecember 1, 2025Eshana Mukhopadhyay, Nora Lakes, Durga Krishnamurthy, Jasmine A Tuazon, David E Ohayon, Ayad Ali, Harsha Seelamneni, Lynn H Lee, David A Hildeman, Stephen N Waggonerthe manufacturer’s protocol, the NK4 program, and electroporation processing assembly SOC25X3 in an ExPERT ATx electroporation system (Maxcyte Inc., Rockville, MD, USA). Quantitative PCR Total RNA was extracted with RNeasy spin columns

(5) Joint single-cell profiling of Cas9 edits and transcriptomes reveals widespread off-target events and effects on gene expressionbioRxivSeptember 24, 2025Michael H. Lorenzini, Brad Balderson, Karthyayani Sajeev, Aaron J. Ho, Graham McVickertransferred to an OC-25×3 process assembly (MaxCyte #SOC-25×3), and immediately transfected with an ExPERT ATx electroporation system (MaxCyte) using cell type-specific instrument protocols provided by MaxCyte (Supp. Table 2). Final electroporation

(6) Systematic discovery of CRISPR-boosted CAR T cell immunotherapiesNatureSeptember 24, 2025Paul Datlinger, Eugenia V. Pankevich, Cosmas D. Arnold, Nicole Pranckevicius, Jenny Lin, Daria Romanovskaia, Moritz Schaefer, Francesco Piras, Anne-Christine Orts, Amelie Nemc, et al.per cell for CRISPR screening in CD8+ T cells. T cell electroporation For electroporation using the ExPERT ATx Electroporator (MaxCyte), cells were pelleted, washed once with room temperature 1× PBS, washed once

(7) Highly efficient gene knockout in tumor-infiltrating lymphocytes by adenine base editingMolecular Therapy OncologySeptember 18, 2025Morteza Hafezi, Raphael Genolet, Leila Hadadi, Bovannak Stewen Chap, Sara Bobisse, Greta Maria Paola Giordano Attianese, Hanan El Jorfi, Daniela Cropp, Laetitia Pericou, Marion Arnaud, et al.supplemented with IL-2 and anti-CD3 monoclonal antibody and subsequently electroporated (EP) using the MaxCyte ExPERT ATx device with ABE mRNA and guide (g)RNA. The EP TILs were then transferred to G-Rex plates containing

(8) Precise correction of G6PD Viangchan mutation in iPSCs by prime editing strategySci RepAugust 18, 2025Chaiwat Netsawang, Malinee Tongbaen, Natee Jearawiriyapaisarn, Kamonlak LeecharoenkiatOpti-MEM Reduced Serum Medium (Gibco). The cell mixture was electroporated using either the MaxCyte ExPERT ATx™ electroporator (program iPSC-XL2; Maxcyte, Inc.), or the Etta Electroporator X-Porator H1 (EBXP-H1;

(9) Med14 phosphorylation shapes genomic response to GLP-1 AgonistbioRxivJune 25, 2025Sam Van de Velde, Jingting Yu, K. Garrett Evensen, Edmund Pakhlevanyan, April E. Williams, Reuben J. Shaw, Marc Montminyug of both LentiCRISPRv2 and repair template plasmid were transfected in INS-1 cells using Maxcyte ExPERT ATx electroporator with Optimization 2 protocol. After transfection, cells were selected in 1 ug/ml puromycin

(10) Effective and stable gene transduction in rhesus macaque iPSCs capable of T-lineage differentiation utilizing the piggyBac systemRegen TherMarch 18, 2024Masahiro Tanaka, Yoshihiro Iwamoto, Bo Wang, Eri Imai, Munehiro Yoshida, Shoichi Iriguchi, Shin Kanekosuspension with 5 μg piggyBac transposon vector and 2 μg pHL-EF1a-hcPBase-A were electroporated by Maxcyte ExPERT ATx. 2.3 Flow cytometry and antibodies The following antibodies were used in this study. The anti CD3

(11) Downregulation of Hypoxia Inducible Factor‐1α in Primary Human Natural Killer Cells Using Small Interfering RNA Delivery with ExPERT ATx by MaxCyteCurrent ProtocolsFebruary 7, 2024Sonia Y. Velásquez, Gizem E. Baslar, Jutta Schulte, Tanja Fuderer, Holger A. Lindner, Anna CoulibalyInducible Factor‐1α in Primary Human Natural Killer Cells Using Small Interfering RNA Delivery with ExPERT ATx by MaxCyte

(12) Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalitiesEBioMedicineJanuary 1, 2024Li N, Xu Y, Chen H, Lin J, AlAbdi L, Bekheirnia MR, Li G, Gofin Y, Bekheirnia N, Faqeih E, et al.EDTA was transfected into patient 1-derived fibroblasts with the dose of 2 μg/250,000 cells using the ExPERT ATx™ electroporation systems according to the manufacturer's instructions (MaxCyte Rockville, MD, USA). Western blot

(13) Rapid identification of neutralizing antibodies against SARS-CoV-2 variants by mRNA displayCell ReportsFebruary 8, 2022Shiho Tanaka et al.SARS-CoV-2-S ECD trimer For transient expression of SARS-CoV-2 S di-Pro ECD timer by Maxcyte® transfection (ExPERT ATx), CHO-S cells were cultured in suspension in CD-CHO media supplemented with 8 mM L-glutamine in shaker

(14) An ACE2 Triple Decoy that neutralizes SARS-CoV-2 shows enhanced affinity for virus variantsScientific ReportsDecember 1, 2021Shiho Tanaka et al.sequence at a concentration of 150 µg/mL, and transfected using OC-400 processing assemblies in a Maxcyte ExPERT ATx Transfection System. Transfected cells were incubated for 30 min at 37 ℃, 5% CO2 and then resuspended

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