Nuclease-Free Water

The presence of nucleases such as DNase and RNase in water can degrade precious molecular samples and even ruin experiments. To prevent DNA and RNA sample loss, it is essential that highly pure, nuclease-free water be used in applications such as PCR, cDNA synthesis, nucleic acid purification, sequencing, and cloning. Methods of removing active nucleases include filtration and treatment with diethylpyrocarbonate (DEPC). DEPC is effective for inactivating ribonucleases, but it may also affect other enzymatic reactions. Thus DEPC-water is not recommended for in vivo and other more delicate applications. Find more information on nuclease-free water by visiting the supplier page.