Primary human cells are directly cultured from their source organ tissue or blood cells. Compared to immortalized cell lines, primary human cells provide enhanced replication of in vivo. Primary cells can be difficult to isolate or to obtain, so a wide range of primary human cells are marketed for diverse research purposes. Bone marrow and peripheral blood cells that express specific CD (cluster of differentiation) markers are available, such as CD34+ cells. Cerebellar granule cells, hippocampal cells, and astrocytes are three cell types out of hundreds that are offered for central nervous system (CNS) studies. Acquiring uncontaminated and pure isolations of human CNS neurons and glial support cells are difficult and time-consuming, so a range of suppliers are available to deliver pre-screened batches of up to 1 million cells per 1ml vial. Highly specific cells that range from eye to gastrointestinal tissue are available for varied scientific research.
Despite its advantages, several challenges need to be resolved.
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A review of novel solutions that enhance mRNA recovery
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Light sheet microscopy allows for fast imaging of large 3D biological samples, like whole rodent organs, embryos or larvae. Compared to other current fluorescence microscopy techniques only a single plane of a labeled sample is illuminated ...
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Discover the beauty of standardized and automated flow cytometry
The MACSQuant® Analyzer 16 is an easy-to-use instrument designed for scalable and reliable flow cytometry assays. This includes features such as:
Full workflow automation: sample ...
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Cells can also be labeled with carboxy-fluorescein diacetate, succimidyl ester, or CFSE, a small fluorescent dye. Although colorless outside of the cell, once a cellular esterase activates this compound, it will label your cells several logs above ...
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Conventional adherent tissue culture involves growing cells on solid flat surfaces as two-dimensional (2-D) monolayers. Although such practices are routine and suitable for transformed or immortalized cell lines, de-differentiation and loss of...
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