Primary human cells are directly cultured from their source organ tissue or blood cells. Compared to immortalized cell lines, primary human cells provide enhanced replication of in vivo. Primary cells can be difficult to isolate or to obtain, so a wide range of primary human cells are marketed for diverse research purposes. Bone marrow and peripheral blood cells that express specific CD (cluster of differentiation) markers are available, such as CD34+ cells. Cerebellar granule cells, hippocampal cells, and astrocytes are three cell types out of hundreds that are offered for central nervous system (CNS) studies. Acquiring uncontaminated and pure isolations of human CNS neurons and glial support cells are difficult and time-consuming, so a range of suppliers are available to deliver pre-screened batches of up to 1 million cells per 1ml vial. Highly specific cells that range from eye to gastrointestinal tissue are available for varied scientific research.
Key choices that support success
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Safer and more reliable media types are increasingly available.
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Distek has earned a place on the modern bioprocessing laboratory benchtop with the BIOne 1250 Single and Dual Bioprocess Control Stations.
Modular, flexible, and highly customizable, the BIOne 1250 systems are ready to support the nuanced ...
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Our InvivO2 workstation is packed with innovative features that allow you to study even the most complex cell interactions under perfect physiological oxygen conditions. Whether you’re hoping to replicate the environment of blood vessels or lung ...
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Cells can also be labeled with carboxy-fluorescein diacetate, succimidyl ester, or CFSE, a small fluorescent dye. Although colorless outside of the cell, once a cellular esterase activates this compound, it will label your cells several logs above ...
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Conventional adherent tissue culture involves growing cells on solid flat surfaces as two-dimensional (2-D) monolayers. Although such practices are routine and suitable for transformed or immortalized cell lines, de-differentiation and loss of...
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