HiScribeT7 Quick High Yield RNA Synthesis Kit from New England Biolabs

Product Specs
ItemHiScribeT7 Quick High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $298.00 Inquire
Catalog NumberE2050S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(61) Engineered circular RNA-based DLL3-targeted CAR-T therapy for small cell lung cancerExp Hematol OncolMarch 12, 2025Jingsheng Cai, Zheng Liu, Shaoyi Chen, Jingwei Zhang, Haoran Li, Xun Wang, Feng Yang, Shaodong Wang, Xiao Li, Yun Li, et al.synthesized via in vitro transcription (IVT) using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S) following the manufacturer's protocol, with incubation at 37 °C for 2 h. The reaction mixtures were

(62) A novel model of central precocious puberty disease: Paternal MKRN3 gene-modified rabbitAnimal models and experimental medicineMarch 1, 2025Chen B, Ye X, Chen L, Liu T, Li G, Sa C, Li J, Liu K, Gu W, Wang G.10 min. After purification of the DNA template, the HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050S, New England Biolabs, USA) was used to carry out the in vitro transcription of sgRNA according to the

(63) Rapid and automated interpretation of CRISPR-Cas13-based lateral flow assay test results using machine learning†Sensors & DiagnosticsFebruary 13, 2025Mengyuan Xue, Diego H. Gonzalez, Emmanuel Osikpa, Xue Gao, Peter B. Lillehojin vitro transcription (IVT) using a HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). IVT templates for the SARS-CoV-2 N gene target were PCR-amplified from gBlock (IDT) containing a T7

(64) Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutioneBioMedicineFebruary 1, 2025Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, et al.from Thermo Fisher Scientific (Waltham, USA). The HiScribeTM T7 Quick High Yield RNA Synthesis Kit (#E2050S) was procured from NEB (New England Biolabs, Ipswich, MA). TaKaRa Ex Taq (#RR001A) was purchased from

(65) Design and Expression of Fasciola hepatica Multiepitope Constructs Using mRNA Vaccine TechnologyInternational Journal of Molecular SciencesJanuary 30, 2025Javier Sánchez-Montejo, Tania Strilets, Raúl Manzano-Román, Julio López-Abán, Mariano A. García-Blanco, Belén Vicente, Antonio Murotemplate for in vitro transcription (IVT) using the HiScribe® T7 Quick High Yield RNA Synthesis Kit yield (E2050S, NEB, Ipswich, MA, USA) with modifications. Firstly, incubation time at 37 °C was increased from 2 to

(66) Nup107 contributes to the maternal to zygotic transition by preventing the premature nuclear export of pri-miRNA 427DevelopmentJanuary 15, 2025Valentyna Kostiuk, Rakib Kabir, Kaitlin Levangie, Stefany Empke, Kimberly Morgan, Nick D. L. Owens, C. Patrick Lusk, Mustafa K. Khokhagenerated using Sp6 and T7, respectively, with the HiScribe High Yield RNA Synthesis Kit (E2070S and E2050S) from New England Biolabs according to the manufacturer's instructions. Plasmids used for probe synthesis

(67) Development and evaluation of a CRISPR/Cas12a-based diagnostic test for rapid detection and genotyping of HR-HPV in clinical specimensMicrobiology SpectrumJanuary 7, 2025Lijuan Yin, Ziqian Zhao, Chunhua Wang, Caihong Zhou, Xiuzhen Wu, Baoxue Gao, Liangyuan Wang, Shuli Man, Xinkuan Cheng, Qiankun Wu, et al.and generation of crRNA were conducted as our previous work (25). T7 High Yield RNA Synthesis Kits (#E2050S) and Monarch RNA Cleanup Kits (#T2040L) were acquired from New England Biolabs (USA). Genomic DNA extraction

(68) Safety and immunogenicity of an optimized self-replicating RNA platform for low dose or single dose vaccine applications: a randomized, open label Phase I study in healthy volunteersNature communicationsJanuary 7, 2025Maine CJ, Miyake-Stoner SJ, Spasova DS, Picarda G, Chou AC, Brand ED, Olesiuk MD, Domingo CC, Little HJ, Goodman TT, et al.in vitro transcription was performed using the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, Cat#E2050S) and purified by LiCl precipitation. The RNA product was capped using Vaccinia Capping System (NEB,

(69) Improved RNA toehold switch sensitivity using the NanoLuc complementation reporterMicroPubl BiolJanuary 2, 2025Katherinne J Diaz, Julian Jarquin, Aleksandr Petrosyan, Melissa K Takahashiin vitro transcribed using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs E2050S) according to manufacturer’s instructions. RNA was purified using the RNA Clean and Concentrator-25

(70) Field-deployable porcine epidemic diarrhea virus diagnostics utilizing CRISPR-Cas13aVirulenceDecember 31, 2024Yuanyuan Wang, Dalin He, Weihua Li, Yaqin Dong, Linlin Fang, Deju Liu, Yi Tang, Shaobo XiaoIVT templates, following the HiScribe T7 Quick High Yield RNA Synthesis Kit instructions (cat. no. E2050S, New England Biolabs). Following a 4-h incubation at 37 °C, the reaction temperature was decreased to

(71) High-throughput specificity profiling of antibody libraries using ribosome display and microfluidicsCell Reports MethodsDecember 16, 2024Ellen K. Wagner, Kyle P. Carter, Yoong Wearn Lim, Geeyun Jenny Chau, Alexis Enstrom, Nicholas P. Wayham, Jessica-Mae Hanners, Chiann-Ling C. Yeh, Marc Fouet, Jackson Leong, et al.template in an in vitro transcription reaction (HiScribe T7 Quick High Yield RNA Synthesis Kit, NEB, E2050S) to generate RNA which subsequently was DNase treated and purified. To generate ARM complexes, 450 nM

(72) Comparison of genotyping assays for detection of targeted CRISPR/Cas mutagenesis in highly polyploid sugarcaneFrontiers in Genome EditingDecember 12, 2024Eleanor J. Brant, David May, Ayman Eid, Fredy AltpeterGeneJET Gel Extraction Kit (K0692; Thermo Fisher Scientific). A HiScribe™ T7 Quick High Yield RNA Synthesis Kit (E2050S; NEB) was used to complete In vitro transcription and DNase I treatment of each sgRNA/scaffold combination.

(73) A conserved transcription factor regulatory program promotes tendon fateDevelopmental CellDecember 1, 2024Xubo Niu, Delmy L. Melendez, Suyash Raj, Junming Cai, Dulanjalee Senadeera, Joseph Mandelbaum, Ilya A. Shestopalov, Scott D. Martin, Leonard I. Zon, Thorsten M. Schlaeger, et al.Kit and sgRNAs were made using HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S). To generate the knockout fish, 1nl of solution (500ng/ul NLS-Cas9-EGFP, GenScript, Z03393; 150ng/ul

(74) Uncovering the transcriptome-wide RNA modifications in Acinetobacter baumanniiMicrobial GenomicsNovember 20, 2024Kah Ern Ten, Sadequr Rahman, Hock Siew Tanthe template for in vitro transcription using HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S) with DNase I treatment according to the kit’s manual. After purification, a second in-tube DNase I

(75) Sequence modeling and design from molecular to genome scale with EvoScienceNovember 15, 2024Eric Nguyen, Michael Poli, Matthew G. Durrant, Brian Kang, Dhruva Katrekar, David B. Li, Liam J. Bartie, Armin W. Thomas, Samuel H. King, Garyk Brixi, et al.the PURExpress (IVTT) kit (NEB E6800S) and the HiScribe T7 High Yield RNA Synthesis (IVT) kit (NEB E2050S), respectively, following the manufacturer’s recommendations. The sgRNA IVT product was column-purified

(76) A single-dose circular RNA vaccine prevents Zika virus infection without enhancing dengue severity in miceNature CommunicationsOctober 16, 2024Xinglong Liu, Zhengfeng Li, Xiaoxia Li, Weixuan Wu, Huadong Jiang, Yufen Zheng, Junjie Zhou, Xianmiao Ye, Junnan Lu, Wei Wang, et al.precursors and control linear precursors were produced using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2050S). Residual DNA templates were digested with DNase I (NEB, M0303S) at 37 °C for 15-30 min. Total RNAs

(77) Structural basis of human 20S proteasome biogenesisNature CommunicationsSeptember 18, 2024Hanxiao Zhang, Chenyu Zhou, Zarith Mohammad, Jianhua ZhaogRNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, E2050S) in 20 μl reactions containing 6 μl Milli-Q water, 1 μl DNA template (50–100 ng total), 10 μl NTP buffer

(78) CRISPR-enabled point-of-care genotyping for APOL1 genetic risk assessmentEMBO Molecular MedicineSeptember 13, 2024Robert Greensmith, Isadora T Lape, Cristian V Riella, Alexander J Schubert, Jakob J Metzger, Anand S Dighe, Xiao Tan, Bernhard Hemmer, Josefine Rau, Sarah Wendlinger, et al.microplate,Corning,CLS3544-50EA HiScribe T7 Quick High Yield RNA Synthesis kit,New England Biolabs,E2050S Monarch RNA Cleanup Kit,New England Biolabs,T2050L RNA Clean & Concentrator 25,Zymo,13175 TwistAmp Basic

(79) Development and characterization of human T-cell receptor (TCR) alpha and beta clones’ library as biological standards and resources for TCR sequencing and engineeringBiology Methods and ProtocolsSeptember 5, 2024Yu-Chun Wei, Mateusz Pospiech, Yiting Meng, Houda Alachkar[36]. DNA transcription Transcription was performed by HiScribe® T7 Quick High Yield RNA Synthesis Kit (#E2050S, NEB, MA, USA) following the manufacturer’s protocol including DNase treatment. Resulting RNA was purified

(80) Single-Molecule Studies of Cognate and Near-Cognate Elongation in an in vitro Eukaryotic Translation SystembioRxivAugust 31, 2024Clark Fritsch, Arpan Bhattacharya, Martin Y. Ng, Hong Li, Philip C. Nelson, Barry S. Cooperman, Yale E. Goldmanthese studies were transcribed from plasmids with the HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050S, New England Biolabs). Transcripts contained a T7 promoter sequence (5' – TAA TAC GAC TCA CTA TAG GG

Be the First to Write a Review!