HiScribeT7 Quick High Yield RNA Synthesis Kit from New England Biolabs

Product Specs
ItemHiScribeT7 Quick High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $298.00 Inquire
Catalog NumberE2050S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(21) An image-based transcriptomics atlas reveals the regional and microbiota-dependent molecular, cellular, and spatial structure of the murine gutCell Host MicrobeFebruary 19, 2026Rosalind J. Xu, Hao Zhang, Paolo Cadinu, Phillip B. Nicol, Uli S. Herrmann, Tyrone Lee, Ludwig Geistlinger, Rafael A. Irizarry, Jeffrey R. MoffittD4004). The product was amplified and converted to RNA with a high yield in vitro transcription (NEB E2050S) and purified with home-made solid-phase reversible immobilization beads39 (based on Cytiva 65152105050250).

(22) Advancing knock-in approaches for robust genome editing in zebrafishBiology OpenFebruary 15, 2026Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I. Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P. Richards, et al.(Genesee Scientific) to generate sgRNA templates. sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen, R1016) according to the manufacturer's instructions.

(23) Genome-wide screening reveals producer-cell modifications that improve virus-like particle production and delivery potencybioRxivFebruary 2, 2026Diana Ly, Hyewon Jang, Adhiraj Goel, Arnav Singh, Aditya Raguramin vitro transcription using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (New England Biolabs; E2050S). Transcribed RNA yield was quantified using the Qubit™ RNA High-Sensitivity Assay Kit (Thermo Fisher Scientific

(24) A rapid, visual, ultrasensitive and highly specific method for detecting adeno-associated virus 2020 based on the LAMP-CRISPR/Cas12a systemPoultry ScienceFebruary 1, 2026Ping Gong, Dagang Tao, Qianhong Chen, Yu Yang, Shengsong Xie, Xing Chen, Suyu Shi, Yanan Wang, Lixia Wang, Yunguo Qian, et al.were transcribed into sgRNAs using the NEB HiScribe™ T7 Quick High Yield RNA Synthesis Kit (Cat No. E2050S, NEW ENGLAND Biolabs). The resulting sgRNAs were then purified and stored for downstream applications.

(25) White spot syndrome virus IE1 protein hijacks the host pentose phosphate pathway to fuel viral replicationPLOS PathogensJanuary 27, 2026Jia Zhang, Kaiyu Lu, Jinghua Zhu, Jude Juventus Aweya, Yueling Zhang, Defu Yaoin vitro using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, USA; Cat. No. E2050S), following the manufacturer’s instructions. The primers used for dsRNA synthesis are listed in S1 Table.

(26) The FANCD2-FANCI heterodimer coordinates chromatin openness and cell cycle progression throughout DNA double-strand break repairCell ReportsJanuary 27, 2026Christine M. Joyce, Julien Bacal, Soham P. Chowdhury, Andrew N. Brown, Amy K. Wang, Carmen Cruz, Kameron Bains, Zachary N. Rodriguez, Nathan J. McCormick, Yaara Tzadikario, et al.transcribed into gRNA by a T7 RNA polymerase using the HiScribe T7 Quick High Yield RNA Synthesis Kit (Cat#E2050S; New England Biolabs), followed by removal of the DNA template by treatment with DNase I. Next, 5′ triphosphate

(27) Hijacking of host Src-PI3K-Akt signaling by WSSV IE1 protein suppresses apoptotic and autophagic defenses to facilitate viral proliferationJournal of VirologyJanuary 20, 2026Kaiyu Lu, Jia Zhang, Jinghua Zhu, Yongzhen Zhao, Xiuli Chen, Yueling Zhang, Defu Yaoin vitro using the HiScribe Quick High Yield RNA Synthesis Kit (New England Biolabs, USA; Cat. No. E2050S) according to the manufacturer’s protocol, with dsEGFP serving as a negative control. For IE1 knockdown,

(28) KLHL13 functional defects cause neurodevelopmental disorder in humans that can be rescued via inhibition of AURKB in cellular and animal modelsGenetics in MedicineJanuary 1, 2026Tehmeena Akhter, Zubair M. Ahmed, Yaping Ji, Axel Schmidt, Meron Azage, Maria Palomares, Kirsten Cremer, Hartmut Engels, Jennifer O. Murphy, Sophia Peters, et al.full-length human KLHL13 mRNA was transcribed using the HiScribe T7 Quick high-yield RNA synthesis kit (Cat#E2050S; NEB). Co-injection of klhl13 ATG_MO and human KLHL13 mRNAs (concentrations: 250pg/μl, 500pg/μl, 750pg/μl,

(29) Imaging the Intestinal Transcriptome With Multiplexed Error‐Robust Fluorescence In Situ Hybridization (MERFISH)Current ProtocolsDecember 27, 2025Paolo Cadinu, Evan Yang, Rosalind J. Xu, Brianna R. Watson, Josh Luce, Jeffrey R. Moffitt(VWR, N2615) Murine RNase inhibitor (NEB, M0314L) HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, E2050S) Maxima H Minus Reverse Transcriptase (200 U/μL; Thermofisher, EP0753) RNase/DNase-free nuclease-free

(30) Endodermal cells use contact inhibition of locomotion to achieve uniform cell dispersal during zebrafish gastrulationiScienceDecember 19, 2025Jesselynn LaBelle, Tom Wyatt, Jaxson Ramirez, Stephanie Wooassays,Critical commercial assays ,, HiScribe T7 quick High Yield RNA synthesis kit,New England Biolabs,E2050S mMessage mMachine T3 transcription kit,ThermoFisher Scientific,AM1344 RNA Clean and Concentrator-25

(31) Furin-Mediated Cleavage of Zona Pellucida Proteins Is Essential for Oocyte DevelopmentMedComm (2020)December 12, 2025Tiechao Ruan, Xiang Wang, Xueguang Zhang, Yan Wang, Chuan Jiang, Sixian Wu, Yunchuan Tian, Xinyao Tang, Jun Ma, Shikun Zhao, et al.mice, and Zp3 Arg353Ser/+ mice. The gRNAs were synthesized with the HiScribe T7 High Yield RNA system (E2050s, NEB) and subsequently purified using MEGAclear Kit (AM1908, Invitrogen) in vitro. Single‐strand oligodeoxynucleotides

(32) Advancing Knock-In Approaches for Robust Genome Editing in ZebrafishbioRxivDecember 12, 2025Anjelica Rodriguez-Parks, Ella Grace Beezley, Steffani Manna, Isabella Silaban, Sarah I Almutawa, Siyang Cao, Hossam Ahmed, Megan Guyer, Sean Baker, Mark P Richards, et al.(Genesee Scientific) to generate sgRNA templates. sgRNAs were synthesized using the HiScribe T7 kit (NEB, E2050S) and purified by the RNA purification Kit (Zymogen, R1016) according to the manufacturer's instructions.

(33) Nanoscale dynamics of enhancer-promoter interactions during exit from pluripotencyNucleic Acids ResDecember 8, 2025Gabriela Stumberger, David Hörl, Dimitra Tsouraki, Clemens Steinek, A Marieke Oudelaar, Heinrich Leonhardt, Hartmann Harzto RNA via a high yield in vitro transcription (IVT) according to the manufacturer’s instructions (#E2050S, New England Biolabs). Each 30 μl reaction consisted of ∼1 μg template DNA, 6.66 μM of each NTP, 20

(34) Near-cognate tRNAs enhance tRNA rejection and prime the ribosome for rapid subsequent tRNA testingBiophysical JournalDecember 2, 2025Clark Fritsch, Arpan Bhattacharya, Martin Y. Ng, Hong Li, Philip C. Nelson, Barry S. Cooperman, Yale E. Goldmanthese studies were transcribed from plasmids with the HiScribe T7 Quick High Yield RNA Synthesis Kit (E2050S, New England Biolabs). Transcripts contained a T7 promoter sequence (5′-TAA TAC GAC TCA CTA TAG GG-3′)

(35) Harnessing mRNA technology against Fasciola hepatica: Immunological insights from a fatty acid binding protein vaccineFrontiers in ImmunologyNovember 25, 2025Javier Sánchez-Montejo, Cristina Teodosio, Tania Strilets, Julio López-Abán, Raúl Manzano-Román, Julio Pozo, Silvia Martin, Lidia Silos, Ignacio Trujillo, Mariano A. García-Blanco, et al.template for in vitro transcription (IVT) using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (E2050S, NEB, Ipswich, MA, USA), incubation for 16 hours at 37°C with 4 mM CleanCap AG (TriLink Biotechnologies,

(36) Systematic discovery of subcellular RNA patterns in the gut epitheliumGenome BiolOctober 29, 2025Minkyoung Lee, Ilhan E. Acar, Davide Eletto, Srivathsan Adivarahan, Farah Mhamedi, Kristina Handler, Jihyun Lee, Elena Guido Vinzoni, Gustavo Aguilar, Andreas E. Moorof the in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit; New England Biolabs, E2050S), performed at 37 °C for 16 h, followed by DNAseI treatment (37 °C for 15 min). IVT RNAs were then cleaned

(37) VGRCOT: a one-tube visual detection method for group B Streptococcus combining RPA and CRISPR/Cas12a for point-of-care testing in reproductive healthMicrobiol SpectrOctober 7, 2025Caixia Ji, Liqiang Ru, Tiao Han, Gang Mai, Laibao Zheng, Yayun Jiangdetection. MATERIALS AND METHODS Reagents LbCas12a (Catalog # M0653T) and RNA transcription Kits (Catalog # E2050S) were purchased from New England Biolabs Inc. (United States). RAA FAST (Catalog # B00000), the recombinase

(38) Pore-Based RNA Evaluation for Control of Integrity, Sequence, and Errors – Quality Control (PRECISE-QC)bioRxivSeptember 26, 2025Yvonne Y. Yee, Dinara Boyko, Bhoomika Pandit, Maksim Royzen, Sara H. Rouhanifard, Meni Wanunuthrough in vitro transcription (IVT) using the HiScribe T7 Quick High Yield RNA Synthesis kit (NEB E2050S). During IVT, 1 μg of template was used and the sample was incubated at 37°C for 2 h. After IVT, the

(39) Joint single-cell profiling of Cas9 edits and transcriptomes reveals widespread off-target events and effects on gene expressionbioRxivSeptember 24, 2025Michael H. Lorenzini, Brad Balderson, Karthyayani Sajeev, Aaron J. Ho, Graham McVickerperformed in an RNase-free environment using the HiScribe T7 Quick RNA synthesis kit (New England BioLabs #E2050S). For IVT on purified gDNA, gDNA was extracted from donor-edited and control cells using a Quick-DNA

(40) SFPQ Promotes Homologous Recombination via mRNA Stabilization of RAD51 and Its ParalogsbioRxivSeptember 16, 2025Sofia Gotthold, Keile R. Hansen, Andrew N. Brown, Soham P. Chowdhury, Hannah I. Ghasemi, Amanda C. Yoon, Christine M. Joyce, Julien Bacal, Brooke M. Gardner, Chris D. Richardsonin vitro using T7 RNA polymerase (HiScribe T7 Quick High Yield RNA Synthesis Kit; New England Biolabs, Cat#E2050S), followed by DNase I treatment to remove residual DNA. The 5′ triphosphate groups were removed by incubation

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