NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(141) Assessing the hodgepodge of non-mapped reads in bacterial transcriptomes: real or artifactual RNA chimeras?BMC GenomicsDecember 1, 2014Verónica Lloréns-Rico, Luis Serrano, Maria Lluch-Senartotal RNA was fragmented to around 100–150 nt using NEB Next Magnesium RNA Fragmentation Module (ref. E6150S, NEB). Treatments with Antarctic phosphatase (ref. M0289S, NEB) and PNK (ref. M0201S, NEB) were performed

(142) Applying thiouracil (TU)-tagging for mouse transcriptome analysisNature ProtocolsFebruary 1, 2014Leslie Gay, Kate V. Karfilis, Michael R. Miller, Chris Q. Doe, Kryn Stankunas)Qubit assay tubes (Invitrogen, cat. no. Q32856)NEBNext magnesium RNA Fragmentation Module (NEB, cat. no. E6150S)Ribo-Zero Magnetic Kit (Epicentre, cat. no. MRZH11124)N,N-Dimethylformamide (Sigma, cat. no. D4551)EZ-Link

(143) Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNAGenes & developmentJanuary 1, 2013Gay L, Miller MR, Ventura PB, Devasthali V, Vue Z, Thompson HL, Temple S, Zong H, Cleary MD, Stankunas K, et al.described above, fragmented using NEBNext Magnesium RNA Fragmentation Module (New England BioLabs, #E6150S) for 4 min at 94°C, purified by RNeasy minikit (Qiagen, #74104), eluted in 50 mL of RNase-free water,

(144) PCIF1 drives oesophageal squamous cell carcinoma progression via m6Am-mediated suppression of MTF2 translationClinical and translational medicineJanuary 1, 0001"Li K, Yi Y, Ling R, Zhang C, Zhang Z, Wang Y, Wang G, Chen J, Cheng M, Chen S."extracted RNA (∼10 µg) was fragmented to obtain ∼100–200 nt fragments using RNA fragmentation buffer (E6150S; NEB) at 70°C for 7.5 min. The fragmented RNAs were then subjected to m7G immunoprecipitation using

(145) N⁶-Methyladenosine Landscape of Glioma Stem-Like Cells: METTL3 Is Essential for the Expression of Actively Transcribed Genes and Sustenance of the Oncogenic SignalingGenesJanuary 1, 0001"Visvanathan A, Patil V, Abdulla S, Hoheisel JD, Somasundaram K."Tris-HCl, pH 7.4. The RNA was subjected to 100bases fragmentation using NEB fragmentation buffer (#E6150S, NEB, Ipswich, MA, USA) for 5 min. The RNA was precipitated using sodium acetate and ethanol. RNA quality

(146) The catalytic efficiency of METTL16 affects cellular processes by governing the intracellular S-adenosylmethionine setpointCell reportsJanuary 1, 0001"Flaherty JN, Sivasudhan E, Tegowski M, Xing Z, McGinnis MM, Hunter OV, Featherston KM, Sethia K, Tu BP, Meyer KD, et al."assays ,, CellTiter-Glo Reagent,ProMega,Cat#G7570 NEBNext Magnesium RNA Fragmentation Module,NEB,Cat#E6150S RiboMinus Eukaryote System V2,ThermoFisher,A15026 NEBNext Small RNA Library Prep Set for Illumina,NEB,E7330S "NEBNext Multiplex Oligos for Illumina, Index primers set 1

(147) Identification of FUBP1 as a Long Tail Cancer Driver and Widespread Regulator of Tumor Suppressor and Oncogene Alternative SplicingCell reportsJanuary 1, 0001"Elman JS, Ni TK, Mengwasser KE, Jin D, Wronski A, Elledge SJ, Kuperwasser C."(MTS),Promega,G3582 TruSeq mRNA Stranded Library Prep,Illumina,20020594 NEBNext Magnesium RNA Fragmentation Module,NEB,E6150S DynaBeads mRNA Purification Kit,ThermoFisher,61006 DynaBeads MyOne Silane,ThermoFisher,37002D protoScript II First Strand cDNA Synthesis Kit

(148) Brain somatic mutations in MTOR reveal translational dysregulations underlying intractable focal epilepsyThe Journal of clinical investigationJanuary 1, 0001"Kim JK, Cho J, Kim SH, Kang HC, Kim DS, Kim VN, Lee JH."via alkaline hydrolysis using the NEBNext Magnesium RNA Fragmentation Module (New England BioLabs, E6150S). Ribosome footprints (~30 nt in size) and RNA fragments (40–60 nt in size) were size-fractionated by

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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