NEBNext Magnesium RNA Fragmentation Module E6150S from New England Biolabs

Product Specs
ItemNEBNext Magnesium RNA Fragmentation Module
CompanyNew England Biolabs
Price $50.00 Inquire
Catalog NumberE6150S
Quantity200 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(121) N⁶-Methyladenosine Landscape of Glioma Stem-Like Cells: METTL3 Is Essential for the Expression of Actively Transcribed Genes and Sustenance of the Oncogenic SignalingGenesFebruary 13, 2019Visvanathan A, Patil V, Abdulla S, Hoheisel JD, Somasundaram K.Tris-HCl, pH 7.4. The RNA was subjected to 100bases fragmentation using NEB fragmentation buffer (#E6150S, NEB, Ipswich, MA, USA) for 5 min. The RNA was precipitated using sodium acetate and ethanol. RNA quality

(122) High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robotProtocol ExchangeMay 24, 2018Josip Herman, Josip Stefan Herman, Sagar -, Dominic Grün\(Applied Biosystems™, 75001.1.ML) 9. NEBNext® Magnesium RNA Fragmentation Module \(New England Biolabs, E6150S) 10. Agencourt® AMPure XP beads \(Beckman Coulter, A63881) 11. Agencourt® RNAclean XP beads \(Beckman Coulter

(123) Baicalin hydrate inhibits cancer progression in nasopharyngeal carcinoma by affecting genome instability and splicingOncotargetJanuary 2, 2018Weiwei Lai et al.fragmented into 200 nt-sized fragments using NEB Next, Magnesium RNA Fragmentation Module (Cat. no. E6150S, NEB) according to the manufacturer’s instructions, and then, the fragments were subjected to three

(124) Selective silencing of euchromatic L1s revealed by genome-wide screens for L1 regulatorsNatureJanuary 1, 2018Nian Liu, Cameron H. Lee, Tomek Swigut, Edward Grow, Bo Gu, Michael C. Bassik, Joanna Wysockamanuals. 100 ng polyA-selected RNA was fragmented with NEBNext Magnesium RNA Fragmentation Module (E6150S, New England Biolabs), and used for first strand cDNA synthesis with SuperScriptII (18064-014, Invitrogen)

(125) Translation complex profile sequencing to study the in vivo dynamics of mRNA–ribosome interactions during translation initiation, elongation and terminationNature ProtocolsApril 1, 2017Nikolay E Shirokikh, Stuart K Archer, Traude H Beilharz, David Powell, Thomas Preisscat. no. Z359947-100EA) • NEBNext Magnesium RNA Fragmentation Module (New England BioLabs, cat. no. E6150S) • Thin-walled polypropylene 89×14 mm ultracentrifuge tubes (Beckman-Coulter, cat. no. 331372) • 80-mm

(126) mRNA N6-methyladenosine methylation of postnatal liver development in pigPLOS ONEMarch 7, 2017Shen He et al.fragmented approximately 150-nucleotide-long using NEBNext1 Magnesium RNA Fragmentation Module (NEB #E6150S) according to the manufacturer’s protocol. Standard ethanol precipitation was performed to precipitate

(127) Rescuing discarded spectra: Full comprehensive analysis of a minimal proteomePROTEOMICSFebruary 1, 2016Maria Lluch-Senar, Francesco M. Mancuso, Héctor Climente-González, Marcia I. Peña-Paz, Eduard Sabido, Luis Serranototal RNA was fragmented to around 100-150 nt using NEB Next Magnesium RNA Fragmentation Module (ref. E6150S, NEB). RNA was treated with Antarctic phosphatase (ref. M0289S, NEB) and PNK (ref. M0201S, NEB) to make

(128) Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafishBMC GenomicsDecember 1, 2015Timothy Erickson, Teresa NicolsonmRNA was fragmented for 4 min at 94 °C using the NEBNext Magnesium RNA Fragmentation Module (NEB – E6150S) to approximately 200–500 bases, recovered by ethanol precipitation, and dissolved in 50 μl RNase-free

(129) Comparative “-omics” in Mycoplasma pneumoniae Clinical Isolates Reveals Key Virulence FactorsPLOS ONESeptember 3, 2015Maria Lluch-Senar, Luca Cozzuto, Jaime Cano, Javier Delgado, Verónica Llórens-Rico, Sabine Pereyre, Cécile Bebear, Luis Serranoof total RNA was fragmented to ~100–150 nt using NEB Next Magnesium RNA Fragmentation Module (ref. E6150S, NEB). Treatments with Antarctic phosphatase (ref. M0289S, NEB) and PNK (ref. M0201S, NEB) were performed

(130) Transcriptome Profiling of Wild-Type and pga-Knockout Mutant Strains Reveal the Role of Exopolysaccharide in Aggregatibacter actinomycetemcomitansPLOS ONEJuly 29, 2015Mayilvahanan Shanmugam, Faiha El Abbar, Narayanan RamasubburRNA-depleted RNA samples were fragmented to ~100 nt using NEBNext Magnesium RNA fragmentation module (Cat. No E6150S, NEB, Ipswich, MA). Strand specific cDNA libraries were prepared by treating fragmented RNA sample to

(131) Assessing the hodgepodge of non-mapped reads in bacterial transcriptomes: real or artifactual RNA chimeras?BMC GenomicsDecember 1, 2014Verónica Lloréns-Rico, Luis Serrano, Maria Lluch-Senartotal RNA was fragmented to around 100–150 nt using NEB Next Magnesium RNA Fragmentation Module (ref. E6150S, NEB). Treatments with Antarctic phosphatase (ref. M0289S, NEB) and PNK (ref. M0201S, NEB) were performed

(132) Applying thiouracil (TU)-tagging for mouse transcriptome analysisNature ProtocolsFebruary 1, 2014Leslie Gay, Kate V. Karfilis, Michael R. Miller, Chris Q. Doe, Kryn Stankunas)Qubit assay tubes (Invitrogen, cat. no. Q32856)NEBNext magnesium RNA Fragmentation Module (NEB, cat. no. E6150S)Ribo-Zero Magnetic Kit (Epicentre, cat. no. MRZH11124)N,N-Dimethylformamide (Sigma, cat. no. D4551)EZ-Link

(133) Mouse TU tagging: a chemical/genetic intersectional method for purifying cell type-specific nascent RNAGenes & developmentJanuary 1, 2013Gay L, Miller MR, Ventura PB, Devasthali V, Vue Z, Thompson HL, Temple S, Zong H, Cleary MD, Stankunas K, et al.described above, fragmented using NEBNext Magnesium RNA Fragmentation Module (New England BioLabs, #E6150S) for 4 min at 94°C, purified by RNeasy minikit (Qiagen, #74104), eluted in 50 mL of RNase-free water,

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NEBNext Magnesium RNA Fragmentation Module

NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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NEBNext Magnesium RNA Fragmentation Module from New England Biolabs

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