HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

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Website: http://www.neb.com

(1) RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted SystemAdv Sci (Weinh)August 21, 2026Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, et al.and Prnp, the in vitro transcription was carried out according to manufacturer's instructions (NEB, E2040S), with the following modifications. Reactions included 1 µg of DNA template and were supplemented with

(2) An integrated nanopore-microfluidic platform for low-voltage electroporation of delivering self-amplifying RNA into dendritic cellsMicrosyst NanoengAugust 17, 2026Bowen Zhang, Yijing Cai, Caiguanxi Deng, Xinshuo Huang, Chuanjie Yao, Xiaotong Li, Lukang Gao, Yujuan Wu, Jinkun Chen, Juan Jiang, et al.MluI (NEB, R3198) as the synthesis template. The RNA was synthesized by using T7 polymerase kit (NEB, E2040S) in cooperation with 2 mM N1-methylpseudouridine-triphosphate (APExBIO, B8049). The precursor RNA was

(3) Defective microhomology-mediated end joining in SMARCB1-deficient tumorsSci AdvAugust 14, 2026Guangli Zhu, Shuhei Asada, Huy Nguyen, Yuna Hirohashi, Lifang Sun, Avneesh Saravanapavan, Erfan Rahmani, Maryia Hrynashka, Sirisha Mukkavalli, Mona Batish, et al.in vitro–transcribed GFP mRNA generated using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, #E2040S) was added as a spike-in to 1 μg of cytoplasmic RNA and 1 μg of nuclear RNA, respectively, prior to

(4) DNA-bound avian R2 non-LTR retrotransposon protein recruits a second R2 protein for genome-protective second-strand nickingProc Natl Acad Sci U S AAugust 11, 2026Adedeji M. Aderounmu, Connor A. Horton, Briana Van Treeck, Anthony Rodríguez-Vargas, Kathleen Collinsdescribed methods (6). In brief, in vitro transcription used the HiScribe T7 High Yield Synthesis Kit (NEB, E2040S). Gel-purified RNA was aliquoted, flash frozen in liquid nitrogen and stored at −80 °C. DNA oligonucleotides

(5) Isothermal assay targeting the V1-V2 region of the 16S rRNA gene for rapid screening of Listeria spp. in raw milkInternational Dairy JournalAugust 11, 2026José E. Barboza-Corona, Sheila A. Martínez-Zavala, Angela P. Mendoza-Valerio, Cynthia P. Rangel-Chávez, Luz E. Casados-Vázquez, Drew Endywas used as a template. The RNA was synthesized and purified with kits from New England Biolabs (NEB, E2040S; T2050). LAMP assays were carried out using either a C1000 Touch thermal cycler (BioRad, Hercules, CA,

(6) Synthetic toehold biosensors for detecting Listeria monocytogenes in raw milkFront MicrobiolJuly 24, 2026Víctor M. Carballo-Uicab, Luz E. Casados-Vázquez, Drew Endy, José E. Barboza-Coronaamplicon was subjected to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After 16 h (overnight) of incubation at 37 °C, the samples were treated with DNase I (RNase-free,

(7) Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seqNature CommunicationsJuly 20, 2026Michihiro Kono, Hiroaki Hatano, Kenichiro Asahara, Masahiro Nakano, Reza Bagherzadeh, Tsugumi Kawashima, Takahiro Arakawa, Miho Sato, Hajime Inokuchi, Takahiro Nishino, et al.(Addgene #178113 and #178114). mRNA was generated by IVT using a HiScribe T7 High-Yield RNA Kit (NEB, E2040S). All mRNA was produced with UTP fully replaced with N1-methylpseudouridine-5’-triphosphate (TriLink,

(8) Genome-wide chromatin recording resolves dynamic cell state changesbioRxivJuly 19, 2026Yodai Takei, Jordan A. Lay, James M. Linton, Duncan M. Chadly, Yoshiki Ochiai, Ron Hadas, Andrew A. Perez, Mario R. Blanco, Paola Laurino, Mitchell Guttman, et al.adenine bases) was prepared by in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (E2040S, NEB) with 1 unit RNase Inhibitor and purified in ribonuclease (RNase)-free water using an RNA Clean & Concentrator-5

(9) A key role for the exoribonuclease XRN1 in regulating the hepatitis B viral transcriptomeiScienceJuly 17, 2026Senko Tsukuda, Nadina Wand, James M. Harris, Olivia Dobrica, Medhi Boutasbih, Xiaxuan Zhu, Peter A.C. Wing, Peter Balfe, Jane A. McKeatingassays,Critical commercial assays ,, HiScribe T7 High Yield RNA Synthesis kit,New England Biolabs,Cat# E2040S HBeAg ELISA,Autobio,Cat# CL0312-2 HBsAg ELISA,Autobio,Cat# CL0310-2 CytoTox 96® Non-Radioactive Cytotoxicity Assay

(10) Transcription initiation drives dosage compensation establishment and maintenance in male DrosophilaSci AdvJuly 17, 2026Ronald Oellers, Anastasios Alexiadis, Yidan Sun, Mohit Navandar, Tanvi Kulkarni, Herbert Holz, Plamen Georgiev, Leily Rabbani, Maria Shvedunova, Niyazi Umut Erdogdu, et al.of cDNA templates using the HiScribe T7 High Yield RNA Synthesis Kit [New England Biolabs (NEB), no. E2040S]. Transfection took place in six-well plates seeded with 1.5 × 106 cells in 1 ml of medium. For each

(11) Iron overload suppresses LKB1 and induces IL36G anti-tumor immunity in PDAC metastasisSci AdvJuly 17, 2026Douglas E. Biancur, Harsha Venkatesh, Amy Crawford, Yealeen Jeong, Albert S.W. Sohn, Kevin S. Kapner, Keisuke Yamamoto, Elaine Y. Lin, Robert S. Banh, Mohamad Assi, et al.of mRNA from the DNA template was performed as per the manufacturer instructions (T7 Hi-Scribe, NEB, E2040S). The mRNA from the in vitro transcription as purified and used for downstream applications. To perform

(12) Overlapping upstream ORFs repress translation and expand proteome diversity in ArabidopsisbioRxivJuly 16, 2026Hsin-Yen Larry Wu, Yu-Hsuan Cheng, Isaiah D. Kaufman, Qiaoyun Ai, Polly Yingshan Hsuin vitro transcription. In vitro transcription was performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) at 37°C for 2 h. The synthesized RNA was treated with 5 U DNase I (Zymo, E1010) at room temperature

(13) Evaluation of a proposed link between the SARS-CoV-2 furin cleavage site and mouse-adapted MERS-coronavirus MA30Proceedings of the National Academy of SciencesJuly 7, 2026Sarah M. Metzger, Terry C. Jones, Jenny I. J. Meier, Anja Richter, Mark-Christian Klassen, Ruth Olmer, Nicolai Diegmüller, Kim C. Heimsch, Christian DrostenPurified templates were in vitro-transcribed using the HiScribe® T7 High Yield RNA Synthesis Kit (NEB #E2040S) according to the manufacturer’s instructions but with an incubation time of 24 h at 25 °C. Anti-reverse

(14) Prokaryotic Schlafen proteins cleave tRNAs during type III CRISPR immunityNature CommunicationsJuly 2, 2026Pedro Weickert, Yang Liu, Jonathan StreckerE. coli genomic DNA) were in vitro transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) and purified with RNA Clean XP beads (Beckman Colter, A63987). Isoleucin tRNA variants were generated

(15) Reciprocal compensation of caspase-2 and p53 regulates the DNA damage response in HCT116 colon cancer cellsJournal of Biological ChemistryJuly 1, 2026Karla E. Lopez, Leslie Bandera, Luiza E. Coutinho, Karen Zúñiga, Sarah Wright, Lisa Bouchier-Hayesby in vitro transcription using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs #E2040S), and RNA was purified using the RNA purification kit (Zymo Research #R1017). 0). Purified sgRNA (0.5

(16) The temporal architecture of the seminiferous epithelial cycle revealed by spatial transcriptomicsCellJune 25, 2026Arun Chakravorty, Jina Yun, Henry Amrhein, Katsuya L. Colón, Toshiyuki Sato, Benjamin D. Simons, Shosei Yoshida, Long Caimanufacturer’s instructions. The PCR products were used as the template for in vitro transcription (E2040S; NEB) followed by reverse transcription (EP7051; Thermo Fisher). After reverse transcription, the probes

(17) Engineering drug-responsive replication machinery for precise control of self-amplifying RNANature Biomedical EngineeringJune 23, 2026Parisa Yousefpour, Justin R. Gregory, Kristen Si, Jan Lonzaric, Yingzhong Li, Junmin Wang, Kashif Qureshi, Amir Ledbetter, Mariane B. Melo, Alexander Hostetler, et al.in vitro transcription (IVT) reactions were performed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040S) with 1–2μg of linear DNA template, scaled as needed. The IVT product was purified using PureLink RNA

(18) Quantitative profiling of RNA modifications enriched in non-membrane-bound cellular structures using APEX-RNA-MSCell Chemical BiologyJune 22, 2026Kyung W. Seo, Dhruv Y. Dhingani, Ralph E. KleinerM0212S). In vitro transcription (IVT) was done using HiScribe T7 High Yield RNA Synthesis kit (NEB, E2040S) and all IVT products were gel purified. For tRNA transfection, 0.3 x 106 HeLa cells were seeded per

(19) Neuronal YTHDF2 suppresses innate immune activation in Aβ pathology by promoting m6A-dependent decay of cytosolic mitochondrial mRNAsSci AdvJune 19, 2026Wenqi Pan, Lin Yang, Yao Zhang, Yan Chen, Yuesi Xu, Yifan Li, Yujie Fu, Chunhui Ma, Chunying Liu, Qing Li, et al.In vitro transcription In vitro RNA was synthesized via the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) following the manufacturer’s instructions. The plasmid template (pGEM-T–mt-Nd4) was synthesized by

(20) Rational design of a modular mRNA vaccine platform for rapid adaptation to SARS-CoV-2 variantsSci RepJune 3, 2026Julia Rudert, Julia Volckmar, Andreas Jeron, Maike Bälkner, Pia Grimpe, Henning Jacobsen, Dandan Zhang, Giovana Bavia Bampi, Luka Cicin-Sain, Dunja Bruder, et al.GenScript. mRNAs were produced using the HiScribe® T7 High Yield RNA Synthesis Kit (New England BioLabs, E2040S) following the manufacturer’s protocol, with minormodifications. In each reaction, uridine was replaced

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7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
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4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

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