HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(81) Somatic deficiency of the human E3 ubiquitin ligase CBL in leukocytes impairs B cell but not T cell development and functionNature immunologyFebruary 1, 2026Vatovec T, Neehus AL, Jackson KJL, Avery DT, Bagarić I, Erazo L, Arango-Franco CA, Ogishi M, Ahmed SF, Cederholm A, et al.PCR product was transcribed using a HiScribe T7 High-Yield RNA Synthesis kit (New England Biolabs, E2040S) according to the manufacturer’s protocol. Uridine was substituted with N1-methylpseudouridine-5′-triphosphate

(82) TMPRSS2 is dispensable for lethal SARS-CoV-2 BA.5 infection in humanized hACE2 golden hamstersEuropean Journal of Medical ResearchJanuary 23, 2026Bowen Wang, Jinwei Zhang, Yuhan Li, Haodong Wang, Mei Dong, Qiu Chen, Aimin Shi, Aihua Zhang, Wentao Zeng, Xuefeng Wang, et al.producing sgRNAs via in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After treatment with lithium chloride precipitation to purify these sgRNAs, they were preserved at

(83) Dynamont: A comprehensive cross-species comparison of ONT segmentation toolsGigaScienceJanuary 21, 2026Jannes Spangenberg, Christian Höner zu zu Siederdissen, Winfried Goettsch, Lennart Köhler, Liz Maria Luke, Kai Papenfort, Manja Marz\begin{document} $\mu$\end{document}L IVT reactions for 1 hour using the HighScribe T7 RNA synthesis Kit (NEB–E2040S), following the manufacturer’s instructions. After IVT, the DNA templates were digested by addition

(84) Defective Microhomology-Mediated End-joining in SMARCB1-Deficient TumorsbioRxivJanuary 16, 2026Guangli Zhu, Shuhei Asada, Huy Nguyen, Yuna Hirohashi, Lifang Sun, Avneesh Saravanapavan, Sirisha Mukkavalli, Geoffrey I. Shapiro, Alan D. D’Andreain vitro–transcribed GFP mRNA generated using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, #E2040S) was added as a spike-in to 1 μg of cytoplasmic RNA and 1 μg of nuclear RNA, respectively, prior to

(85) Untangling overlapping barcodes in spatial genomics databioRxivJanuary 16, 2026Jonathan A. White, Chuqi Lu, Lincoln Ombelets, Long Caiwere purified using QIAquick PCR Purification Kit (Qiagen 28104). Then, in vitro transcription (NEB E2040S) was performed, followed by reverse transcription (Thermo Fisher EP0751). After reverse transcription,

(86) Enhanced SfaTnpB enables single-base-specific, one-pot nucleic acid detection for high-sensitivity diagnosticsNucleic Acids ResearchJanuary 8, 2026Bingrong Xu, Sheng Li, Yong Li, Shuhong Zhao, Xinyun Li, Jianlin Han, Di Wu, Shuaicheng Li, Ling Chen, Shengsong Xie, et al.templates for in vitro transcription using the HiScribeTM T7 High Yield RNA Synthesis Kit (NEB, Cat. No. E2040S, USA) according to the manufacturer’s instructions. For the preparation of separate scaffold and spacer

(87) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.templates for in vitro transcription (IVT) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB #E2040S). IVT products were separated by size-exclusion chromatography on a Superdex 200 increase 10/300 GL

(88) Lineage-determining transcription factors constrain cohesin to drive multi-enhancer oncogene regulationNature Cell BiologyJanuary 1, 2026Yeqiao Zhou, Atishay Jay, Noah Burget, Tobias Friedrich, Sora Yoon, Jessica Alsing, Guy Nir, Rudolf Grosschedl, Golnaz Vahedi, Robert B. Faryabiwith Zymo DNA Clean & Concentrator-5 (cat. no. 4013), and T7 in vitro transcription (NEB, cat. no. E2040S) was performed at 37 °C overnight. Reverse transcription was performed with maxima RT-H (Thermo, cat.

(89) Robust CD8(+) T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFrontiers in immunologyJanuary 1, 2026Li K, Li X, Zhang C, Dai S, Li L, Wang J, Zhang H, Zhao L.Thermo Scientific, USA) and served as templates for in vitro transcription employing T7 RNA polymerase (#E2040S, New England Biolabs, USA). During transcription, m1ψ-5’-triphosphate (#N-1081-10, TriLink, USA) was

(90) Disruption of the nucleocapsid-RNA condensation by punicalagin is a broad-spectrum antiviral approachFundamental ResearchJanuary 1, 2026Shao-Zhen Jiang, Liu-Bing Yu, Beilei Shen, Jie Pan, Xiao-Wei Li, Rong-Bo Luo, Ling-Jun Fan, Bing-Shuo Qian, Yunkai Zhu, Ziqiao Wang, et al.was in vitro transcribed and labeled with HyperScribe T7 High Yield Cyanine 5 (Cy5) RNA Labeling Kit (E2040S, NEB). Cy5-labeled 10-nt to 60-nt vRNA (segment of Nsp1) was generated by Tsingke Biological Technology.

(91) Glucocorticoid Receptor Activation Reprograms NK Cells to Drive AREG‐Mediated Immunosuppression: A Pan‐Cancer Role for AREGAdvanced ScienceJanuary 1, 2026Qin Wei, Guirong Liang, Rui zeng, Yuancheng Li, Anlan Hong, Hongsheng Wang, Suying Feng, Yan Wang, Yetao Wangwas subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After ExoSAP‐IT (Affymetrix, 78200) treatment, the IVT RNA was fragmented using RNA fragmentation reagents

(92) Reconstructing the three-dimensional architecture of extrachromosomal DNA with ec3DNature CommunicationsDecember 20, 2025Biswanath Chowdhury, Kaiyuan Zhu, Chaohui Li, Jessica Alsing, Jens Luebeck, Maria E. Stefanova, Owen S. Chapman, Katerina Kraft, Shu Zhang, Jun Yi Stanley Lim, et al.ultra-pure water. The dsDNA product was then in vitro transcribed into RNA at 37 °C for 16 h (NEB HiScribe, E2040S). The RNA was then reverse transcribed into cDNA using Maxima H Minus Reverse Transcriptase (Thermo Fisher

(93) The conserved SEN1 DNA/RNA helicase has multiple functions during yeast meiosisPLoS GenetDecember 11, 2025Robert Gaglione, Leonidas Pierrakeas, Lihong Wan, Jonathan Caradonna, Amy J. MacQueen, Ed Luk, Nancy M. Hollingsworthfragment was transcribed using the HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs #E2040S;). Biotin was incorporated into nascent RNA transcripts using a 1:3 ratio of Biotin-16-UTP (Roche #11388908910):UTP.

(94) Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5STAR ProtocolsDecember 1, 2025Joe D. Joiner, Alba Herrero del Valle, Rahul Singh, Yorgo Modis(Invitrogen, cat. no. AM1333) or HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S), according to the manufacturer’s instructions.3.DNase digestion to remove the DNA template.a.Add 1–2

(95) Novel quantitative and specific RT-qPCR assay for UK subtypes of European strain of Tick-borne encephalitis virusVirus ResearchDecember 1, 2025Mollie Curran-French, Jake D’Addiego, Stuart Dent, Gillian Slack, Kyle Perrins, Fern Jenkins, Nyah Davis, Roger Hewsonpurification kit (Qiagen 28104). RNA was transcribed using the HiScribe T7 High yield RNA synthesis kit (NEB E2040S). Briefly, DNA was incubated with T7 polymerase at 37C for 2 h followed by DNAase I (NEB 0303) treatment

(96) An unbiased proteomic platform for ATE1-based arginylation profilingNature Chemical BiologyDecember 1, 2025Zongtao Lin, Yixuan Xie, Joanna Gongora, Xingyu Liu, Emily Zahn, Bibhuti Bhusana Palai, Daniel H. Ramirez, Richard M. Searfoss, Francisca N. Vitorino, Rashmi Karki, et al.following the manufacturer’s protocol on HiScribe T7 High Yield RNA Synthesis Kit (NEB, catalog no. E2040S) using 250 ng of DNA template for a 20–µl reaction incubated at 37 °C for 4 h. DNase and its buffer

(97) PyFuRNAce: an integrated design engine for RNA origamiNat CommunDecember 1, 2025L. Monari, I. Braun, W. Verstraeten, E. Poppleton, K. GöpfrichmM sucrose) and a 1:10 volumetric dilution of the HiScribe T7 RNA Polymerase Mix from NEB (cat. no. E2040S) and a final DNA concentration of 2,5 ng/μL. 50 μM of DFHBI-1T (Sigma-Aldrich; cat. no. SML2697-5MG)

(98) BeadBuddy: user-friendly, nanometer-scale registration of single-molecule imaging databioRxivNovember 30, 2025Finnegan T Clark, Peter H Whitney, Néstor Saiz, Angelina Ziarno, Timothée Lionnetdouble-stranded DNA templates were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Cat. No. E2040S) at 37 °C for 4–16 h (saturating product). RNA was purified using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel,

(99) A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biologyDevelopmental CellNovember 20, 2025Jingbo Sun, Irem Sude Atiş, Stéfany L. L. Empke, Mustafa K. Khokha, David K. BreslowDigoxigenin-labeled antisense RNA probes were synthesized using the T7 High Yield RNA Synthesis Kit (E2040S; New England Biolabs). Embryos were fixed in MEMFA for 1–2 h at room temperature and subsequently dehydrated

(100) Alternative start codon selection shapes mitochondrial function and rare human diseasesMolecular CellNovember 20, 2025Jimmy Ly, Matteo Di Bernardo, Yi Fei Tao, Ekaterina Khalizeva, Christopher J. Giuliano, Sebastian Lourido, Mark D. Fleming, Iain M. CheesemanPCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. To remove free nucleotides and abortive transcripts, the reaction

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7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
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4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

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