HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(81) Novel quantitative and specific RT-qPCR assay for UK subtypes of European strain of Tick-borne encephalitis virusVirus ResearchDecember 1, 2025Mollie Curran-French, Jake D’Addiego, Stuart Dent, Gillian Slack, Kyle Perrins, Fern Jenkins, Nyah Davis, Roger Hewsonpurification kit (Qiagen 28104). RNA was transcribed using the HiScribe T7 High yield RNA synthesis kit (NEB E2040S). Briefly, DNA was incubated with T7 polymerase at 37C for 2 h followed by DNAase I (NEB 0303) treatment

(82) Protocol for the expression, purification, and biochemical characterization of the innate immune sensor MDA5STAR ProtocolsDecember 1, 2025Joe D. Joiner, Alba Herrero del Valle, Rahul Singh, Yorgo Modis(Invitrogen, cat. no. AM1333) or HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S), according to the manufacturer’s instructions.3.DNase digestion to remove the DNA template.a.Add 1–2

(83) BeadBuddy: user-friendly, nanometer-scale registration of single-molecule imaging databioRxivNovember 30, 2025Finnegan T Clark, Peter H Whitney, Néstor Saiz, Angelina Ziarno, Timothée Lionnetdouble-stranded DNA templates were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Cat. No. E2040S) at 37 °C for 4–16 h (saturating product). RNA was purified using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel,

(84) Alternative start codon selection shapes mitochondrial function and rare human diseasesMolecular CellNovember 20, 2025Jimmy Ly, Matteo Di Bernardo, Yi Fei Tao, Ekaterina Khalizeva, Christopher J. Giuliano, Sebastian Lourido, Mark D. Fleming, Iain M. CheesemanPCR product was used in an in vitro reaction with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) in the presence of 20 U/ml SUPERaseIn. To remove free nucleotides and abortive transcripts, the reaction

(85) A microscopy-based CRISPR screening platform enables organellar functional genomics and illuminates ciliary biologyDevelopmental CellNovember 20, 2025Jingbo Sun, Irem Sude Atiş, Stéfany L. L. Empke, Mustafa K. Khokha, David K. BreslowDigoxigenin-labeled antisense RNA probes were synthesized using the T7 High Yield RNA Synthesis Kit (E2040S; New England Biolabs). Embryos were fixed in MEMFA for 1–2 h at room temperature and subsequently dehydrated

(86) Deep generative optimization of mRNA codon sequences for enhanced mRNA translation and therapeutic efficacyNature CommunicationsNovember 12, 2025Yupeng Li, Fan Wang, Jiaqi Yang, Zirong Han, Linfeng Chen, Wenbing Jiang, Hao Zhou, Tong Li, Zehua Tang, Jianxiang Deng, et al.circular mRNA plasmid templates with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S) following the kit manual. After IVT, circular mRNA was purified using the Monarch RNA Cleanup Kit (New England Biolabs

(87) The Tip60 acetylome is a hallmark of the proliferative state in DrosophilaNucleic Acids ResearchNovember 5, 2025Zivkos Apostolou, Anuroop V Venkatasubramani, Lara C Kopp, Gizem Kars, Alessandro Scacchetti, Aline Campos-Sparr, Tamas Schauer, Axel Imhof, Peter B Beckergenerated by in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Cat. No. E2040S). Primer sequences can be found in Supplementary Table 1. For CUT&RUN, the cells underwent two consecutive

(88) NAC couples protein synthesis with nascent polypeptide myristoylation on the ribosomeThe EMBO journalNovember 1, 2025Zdancewicz S, Maldosevic E, Malezyna K, Jomaa A.28104) and were in vitro transcribed using either a HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) or with 1.1 mg/mL T7 polymerase in reaction buffer (40 mM Tris-HCl pH 7.6, 5 mM ribonucleoside triphosphates,

(89) A cadherin-integrin–ECM code for presomitic mesoderm fluidityDevelopmentNovember 1, 2025Miriam A. Genuth, Dörthe Jülich, Andrew T. Ton, Sarah J. Smith, Emilie Guillon, Mark D. Shattuck, Corey S. O'Hern, Scott A. Holley(2013) and the gRNA synthesized with HiScribe T7 according to the manufacturer's instruction (NEB, E2040S). To generate the donor plasmid, approximately 150 bp of genomic sequence flanking the insertion site

(90) Integrated epigenetic and genetic programming of primary human T cellsNature biotechnologyOctober 21, 2025Goudy L, Ha A, Borah AA, Umhoefer JM, Chow L, Tran C, Winters A, Talbot A, Hernandez R, Li Z, et al.IVT reactions were performed with the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs, E2040S) under full substitution of pseudo-UTP and in presence of 4 mM CleanCap AG (which encodes Cap1) (TriLink

(91) A novel mechanism by which c-MYC is aberrantly activated by epigenetic silencing of its antisense lncRNA in colon cancerFrontiers in GeneticsOctober 21, 2025Xuming Hu, Ye Wei, Meiying Zhang, Chunfeng Dou, Liping Wang, Gul Zaib, Huixian Wu, Wang Guo, Xiaoyuan Wang, Shihao Chen, et al.in vitro transcription was performed using the T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S), and RNA purification was performed by a Monarch® RNA Cleanup Kit (New England Biolabs, T2040). The

(92) Enhancing cap-independent translation of linear mRNANat CommunOctober 16, 2025Sebastian Golojuch, Brendan Largey, Afaf H. El-Sagheer, Tom Brownyielding 5′-triphosphate transcripts were carried out using HiScribe® T7 High Yield RNA Synthesis Kit (#E2040S, New England Biolabs). For 20 μL reaction, water (6.0 μL), 10X reaction buffer (2.0 μL), ATP (100 mM,

(93) Dysregulation of AGO2-miRNA dynamics underlies the AGO2-associated Lessel-Kreienkamp syndromeNucleic Acids ResOctober 16, 2025TingYu M Liu, Debora Tibbe, Jan Broder Engler, Irm Hermans-Borgmeyer, Uwe Borgmeyer, Kerstin Robles de Maruri, Davor Lessel, Ian J MacRae, Hans-Jürgen KreienkampFisher Scientific). Transcription was performed using the HiScribe™ T7 High Yield RNA Synthesis Kit (#E2040S, New England Biolabs), with subsequent purification of the transcript with the MEGAClear™ Transcription Clean-Up Kit

(94) Mismatch-introduced crRNA guided PCR-CRISPR/Cas12a platform improves EGFR point mutation detection in single tumor cellMikrochim ActaOctober 11, 2025Meng Wu, Fang Wang, Yu Wang, Ya-Xian Wu, Bo-Yu Tian, Xin-Yuan Ou, Qin-Chen Xu, Xiao-Yan Wu, Chao Han, Wan-Li Liu, et al.transcribed at 37 °C using the HiScribe T7 High-yield RNA Synthesis Kit (New England Biolabs, MA, USA; E2040S). DNase Ι (New England Biolabs, MA, USA; M0303S) was used to remove the template DNA, and the crRNA

(95) Precisely defining disease variant effects in CRISPR-edited single cellsNatureOctober 2, 2025Yuriy Baglaenko, Zepeng Mu, Michelle Curtis, Hafsa M. Mire, Vidyashree Jayanthi, Majd Al Suqri, Cassidy Liu, Ryan Agnew, Aparna Nathan, Annelise Yoo Mah-Som, et al.mRNAs were generated by in vitro transcription using the HiScribe T7 High-Yield RNA synthesis kit (NEB, E2040S) by the method described previously49. NEBnext polymerase was used to PCR amplify template plasmids

(96) Sulfonium lipid nanoparticles for intranasal mRNA delivery to lung epithelial and immune cellsActa BiomaterialiaOctober 1, 2025Yuqin Men, David O. Popoola, Zhi Cao, Yiran Li, Stephan Wilkens, Yong Teng, Qinghe Meng, Marc Hershenson, Yamin LiBioTechnologies). For IL-10 mRNA synthesis, the HiScribe T7 High-Yield RNA Synthesis Kit (New England Biolabs, Cat# E2040S) was used following the manufacturer’s instructions. Briefly, the pMRNA-Il10–3UTRs plasmid was used

(97) RNA Sequencing at Single Vesicle Resolution via 3D Printed Embedded Droplet ArraysACS Applied Materials & InterfacesSeptember 24, 2025Andrew A. Brock, Senthilkumar Duraivel, Jinmai Jiang, Jonathan Fischer, Zachary F. Greenberg, Mei He, Thomas E. Angelini, Thomas D. Schmittgen(IVT) was performed overnight (15 h at 37 °C) using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), and purified with 1.3× AMPure XP beads. The amplified RNA (aRNA) was fragmented at 70 °C for 2 min

(98) High-efficiency homology-directed insertion into the genome using the engineered homing endonuclease ARCUSNucleic acids researchSeptember 23, 2025Resly LC, Tubbs AL, Vogel AJ, Hux JA, MacDonald IA, Harris J, Mischler A, Tomberlin GH, Evans KS, Thompson R, et al.were used as template for in vitro transcription (IVT) using a HiScribe T7 kit (New England biolabs, E2040S). A co-transcriptional capping RNA synthesis procedure was performed according to the manufacture’s

(99) Scalable generation and functional classification of genetic variants in inborn errors of immunity to accelerate clinical diagnosis and treatmentCellSeptember 4, 2025Zachary H. Walsh, Chris J. Frangieh, Neeharika Kothapalli, Jay Levy, Clarissa K. Heck, Johannes C. Melms, Ron S. Gejman, Parin Shah, Jared M. Pollard, Akul Naik, et al.and in-vitro transcribed using the HiScribe® T7 High-Yield RNA Synthesis Kit (New England Biolabs, #E2040S) in a total reaction volume of 320μl, with full substitution of N-1-methyl-pseudouridine-5’-triphosphate

(100) TDP-43 binds to RNA G-quadruplex structure and regulates mRNA stability and translationNucleic Acids ResearchSeptember 3, 2025Jieyu Zhao, Feng Yang, Yuwei Zhang, Huating Wang, Chun Kit Kwok(Thermo Fisher Scientific, 18080085). Then, the random RNA library was prepared and purified by in vitro transcription (NEB, E2040S) and incubated with an increasing concentration of TDP-43 (0, 5, 20, 80, 320, and 1300 nM). The RNA

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7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
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4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

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