HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) RNA promotes synapsin phase separation providing a platform for local translationbioRxivApril 11, 2026Branislava Rankovic, Zachary M Geisterfer, Akshita Chhabra, Vladimir M Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, et al.and Prnp, the in vitro transcription was carried out according to manufacturer’s instructions (NEB, E2040S), with the following modifications. Reactions included 1 μg of DNA template and were supplemented with

(42) Nascent transcript O-MAP reveals the molecular architecture of a single-locus subnuclear compartment built by RBM20 and the TTN RNAbioRxivApril 8, 2026Evan E. Kania, Aidan Fenix, Daphnée M. Marciniak, Qiaoyi Lin, Sara Bianchi, Borislav Hristov, Shuai Li, Conor K. Camplisson, Rose Fields, Brian J. Beliveau, et al.used as templates for in vitro RNA synthesis using the HiScribe T7 High Yield RNA Synthesis Kit (NEB; E2040S), in a final reaction volume of 30 μL, following the manufacturer’s provided protocol. Reactions were

(43) Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responsesSci RepApril 2, 2026Ruohan Yang, Lin Jia, Jiuwei Cui(Sigma-Aldrich, F1804). circRNA was synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S). IVT templates were amplified via PCR before RNA synthesis (Q5 Hot Start High Fidelity 2x Master Mix).

(44) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.Mitochondria Isolation Kit for Cultured Cells,Thermo Fisher,89874 EasyPep kit,Thermo Fisher,A40006 HiScribe T7 RNA synthesis kit,NEB,E2040S Monarch RNA cleanup kit,NEB,T2040L DNA Clean & Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit

(45) Single-cell phenotypic heterogeneity shapes quorum signaling dynamics in Pseudomonas aeruginosaNature CommunicationsMarch 31, 2026Danielle G. Lange, Vadim Litvinov, Daniel DarAgilent DNA TapeStation. 3 µg of purified DNA were transcribed in vitro with the HiScribe T7 kit (NEB, E2040S) according to the manufacturer’s short-transcript protocol (8–12 h, 37 °C). 5 µL of RNA was column-purified

(46) lncRNA ch-MYC-AS1 restricts ALV-J replication by disrupting the ANXA2-C-Myc oncogenic axisMicrobiology SpectrumMarch 30, 2026Suyu Fan, Weiyi Zhou, Xuming Hu, Yingjie Gu, Guangzhong Peng, Yu Zhang, Wenming Zhao, Guohong Chen, Qi Xuin vitro transcription was performed using the T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S), and RNA purification was performed by a Monarch RNA Cleanup Kit (New England Biolabs, T2040). The

(47) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekdishes and transfected the following day with 1 μg of circRNA, generated by IVT (NEB HiScribe T7 kit, E2040S) and subsequently purified using RNase R (Biozym, 172010) digestion with or without prior 4% urea-PAGE

(48) A rapid and highly sensitive CRISPR-Cas12a ortholog-assisted assay for genotyping of myostatin knockout pigsCell & BioscienceMarch 29, 2026Yuan Wang, Hui Yang, Wenhua Zhang, Dagang Tao, Suyu Shi, Sheng Li, Xiao Wu, Yunlong Ma, Jinxue Ruan, Lu Jing, et al.purified and transcribed overnight at 37 °C using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). The resulting RNA was cleaned with the Monarch RNA Cleanup Kit (NEB, T2040S). Purified crRNA concentrations

(49) A Biochemical Analysis of LINC00896 RNA in Cortex Neuronal Cells and Its Possible Connection to the Development of AutismJournal of Nucleic AcidsMarch 28, 2026Rachel A. Hellmann Whitaker, Joshua J. Storey, Isabella Vannavong, Robert S. Mangiamele, Micalyn A. Trihus, Donovan D. Floyd, Trevor Rohder, Dylan L. Cleveland, Andrew Rizeareproducibility. 2. Materials and Methods 2.1. Materials HiScribe T7 High Yield RNA Synthesis Kit E2040S (New England BioLabs, Ipswich, MA) was used to in vitro transcribe LINC00896 RNA via runoff transcription

(50) A molecular switch in NAC prevents mitochondrial protein mistargeting by SRPNature CommunicationsMarch 27, 2026Emir Maldosevic, Radoslaw Jakub Gora, Liangguang Leo Lin, Linyao Elina Zhou, Zexin Jason Li, Yelena Peskova, Ling Qi, Shu-ou Shan, Ahmad JomaaMgCl2, 2 mM spermidine, 1 mM DTT, 0.04 U/µL RNase Inhibitor) or with the T7 HiScribe kit (NEB, cat no. E2040S). Following the reaction period, the precipitate was pelleted at ~22,000 × g for 5 min at 4 °C. The

(51) RNA functional modulation by Mitoxantrone via RNA structural ensemble repartitioningNature CommunicationsMarch 23, 2026Chundan Zhang, Ivana Borovská, Teona Iobashvili, Edoardo Morandi, Marta Lionnez, Oluwatosin S. Olayinka, Rinse de Boer, Massimiliano Clamer, Martin D. Witte, Klaus Pors, et al.nt exon 2, 0.2 μl T7 RNA Polymerase Mix (part of the HiScribe® T7 High Yield RNA Synthesis Kit; cat. E2040S, New England Biolabs), 0.1 μl SUPERase•In™ RNase Inhibitor (cat. A2696, ThermoFisher Scientific), 0.5

(52) C9orf72 hexanucleotide repeat RNA drives transcriptional dysregulation through genome-wide DNA:RNA hybrid G-quadruplexesNeuronMarch 18, 2026Honghe Liu, Mingming Liu, Yang Liu, Gege Gui, Tapas Paul, Yu-Ning Lu, Zhiyuan Huang, Haocheng Wang, Yatao Xiao, Zhongfan Zheng, et al.respectively. The linearized DNA was transcribed with a HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), followed by RNA purification using RNA Clean & Concentrator™-25 (Zymo Research, R1017). To make template

(53) Sequence-specific trapping of EF-Tu/glycyl-tRNA complex on the ribosome by bottromycinbioRxivMarch 16, 2026Dmitrii Y. Travin, Ritwika S. Basu, Madhura N. Paranjpe, Dorota Klepacki, Anna I. Zhurakovskaya, Nora Vázquez-Laslop, Alexander S. Mankin, Yury S. Polikanov, Matthieu G. Gagnon70S ribosomes. The mRNA template was in vitro transcribed (HiScribe T7 RNA synthesis kit, Cat. No. E2040S, NEB) from a PCR fragment containing the dapG sequence, as described in the toeprinting assay. 5 μL

(54) MERFISH+, a large-scale, multi-omics spatial technology resolves the molecular holograms of the 3D human developing heartbioRxivMarch 11, 2026Colin Kern, Qingquan Zhang, Yifan Lu, Jacqueline Eschbach, Zehua Zeng, Elie N. Farah, Chu-Yi Tai, Kaifu Yang, Ignatius Jenie, Fenyong Yao, et al.templates were converted into RNAs using the in vitro T7 transcription reaction (New England Biolabs, E2040S). These RNAs were then converted back to single-stranded DNA using reverse transcription with a concentration

(55) A dual-reporter mouse for therapeutic discovery in Angelman syndromeJCI InsightMarch 9, 2026Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, et al.[UNC] Animal Models Core), transcribed in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs), and purified via RNeasy spin columns (74104, QIAGEN) in microinjection buffer

(56) Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcomaEMBO Molecular MedicineMarch 6, 2026Sophie A Richardson, Aram Safrastyan, Mina Karimpour, Gayatri Gulati, Patrick J B Harker, Alan Redfern, Simon P Pearce, Vsevolod J Makeev, Bernadette Brennan, Alexander T J Lee, et al.Sensifast SYBR Hi-ROX Kit,Bioline (SLS),BIO-92005 HiScribe T7 High Yield RNA Synthesis Kit,New England Biolabs,E2040S Agencourt AMPure XP,Beckman Coulter,A63881 FastAP Thermosensitive Phosphatase,Thermo Fisher,EF0654 "T4 RNA Ligase 2, Truncated KQ

(57) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. Gladfeltertranslation-competent Nluc or MoonTag mRNAs were carried out according to manufacturer’s specifications (NEB, E2080S or E2040S). The cDNA template was amplified using PCR for all Nluc constructs (primers given in Supplementary

(58) An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencingSTAR ProtocolsMarch 1, 2026Kamil Więcek, Janusz Wiśniewski, Heng-Chang Chen16500500 T7 RNA polymerase mix (supplied in HiScribe T7 High Yield RNA Synthesis kit),New England Biolabs,E2040S 100 mM ATP (supplied in HiScribe T7 High Yield RNA Synthesis kit),New England Biolabs,E2040S 100 mM

(59) Diverse database and machine learning model to narrow the generalization gap in RNA structure predictionScience AdvancesFebruary 27, 2026Albéric A. de Lajarte, Yves J. Martin des des Taillades, Justin Aruda, Pierre Bongrand, Federico Fuchs Wightman, Dragui Salazar, Matthew F. Allan, Colin Kalicki, Casper L’Esperance-Kerckhoff, Alex Kashi, et al.in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, catalog no. E2040S) kit according to the manufacturer’s instructions. Following DNase digestion (Invitrogen, catalog no.

(60) Scalable and multimodal brain angiogenesis and blood-brain barrier genetics by somatic mutagenesisCommunications BiologyFebruary 25, 2026Jay Mehul Panji, Raoul Freitas Vale Germano, Michelle America, Liang Lou, Laurence Lecordier, Patricia Tebabi, Maud Martin, Benoit Vanhollebekeas published previously15 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S). The following sgRNA target sites were used: cldn5a_1: 5’-GCGCGGGTGGTGAACGTCGGAGG-3’, cldn5a_2: 5’-GGACAACGTGAAAGCGCGGGTGG-3’,

Write a Review

(1)

7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
(1)

4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (564)

Reviews

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map