HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Declining ribosome function limits oocyte longevitybioRxivOctober 23, 2026Kayla Judson, Katie J. Danielson, Shuang Tang, Sahil Chandhok, Elizabeth Hui, Fiona Wong, Thibault Mayor, Karen Schindler, Ethan J. Greenblattwith BamH1 restriction enzyme and in vitro transcribed with T7 RNA polymerase (New England Biolabs, E2040S) and post-transcriptionally 5’ capped (Cellscript, C-SCCE0625) and polyA-tailed (CellScript, C-PAP5104H).

(2) Antiangiogenic Drug Testing Using Proangiogenic and Hypoxia Zebrafish ModelBio ProtocOctober 20, 2026S. Vinoth, Kirankumar Santhakumarnumber: A180301) 3. HiScribe T7 High Yield RNA Synthesis kit (NEW ENGLAND Biolabs, catalog number: E2040S) (store at -20 °C) 4. mMESSAGE mMACHINE SP6 kit (Invitrogen, catalog number: AM1344) (store at -20 °C) 5.

(3) Regulatable in vivo gene expression via adaptamersMol CellOctober 20, 2026Jack Bryant, Laura Herron, Yesh Doctor, Finn McRae, Jocelyn Wang, Theodore Hirsch, Andrew Portell, Satheesh Kumar, Prashant MaliBright-Glo™ Luciferase Assay System,Promega,E2610 RiboGreen®,ThermoFisher,R11491 Purified ADAR1 p150 protein,BPS Bioscience,100472-1 HiScribe® T7 High Yield RNA Synthesis Kit,NEB,E2040S DTT,NEB,B1222AVIAL RNAse Inhibitor,ThermoFisher,N8080119 Glo Lysis Buffer,Promega,E2661 Trizol,ThermoFisher,15596026 Polyethylene

(4) A B1 SINE element within the mouse Malat1 long noncoding RNA acts as an internal ribosome entry site mediating micropeptide translationSci AdvOctober 16, 2026Wen Xiao, Tanja Hann, Zion Perry, Vijaya Pandey, Jeffrey Cheng, James Wohlschlegel, Anna Marie Pyle, Douglas L. Blackgenerated by in vitro transcription. mRNAs were in vitro transcribed using the HiScript T7 kit (NEB, E2040S). A 40-μl transcription reaction contained 2 μg of linearized DNA templates, 4 mM of each NTP, 3 μl

(5) HOMEBRED: A unified CRISPR platform for field-ready shadowing of infectious agents and oncogenic mutationsiScienceOctober 16, 2026Adnan Asadbeigi, Hassan Fazilaty, Mojtaba Saffari, Reza Shirkoohi, Mohammad Hossein Modarressi, Abdoalreza Salehi, Mohammad Reza Bakhtiarizadehproteins","Chemicals, peptides, and recombinant proteins" ,, HiScribe T7 High Yield RNA Synthesis Kit,NEB,Cat# E2040S RNase-free DNase I,Thermo Fisher Scientific,Cat# EN0521 Monarch RNA Cleanup Kit,NEB,Cat# T2030S Dulbecco’s

(6) Molecular insights into the promiscuous Ap4N hydrolase YqeKJournal of Biological ChemistryOctober 1, 2026Renuka Dharani Shivakumar, Natalie Happel, Fabiana Burchert, Michel Brück, Lennart Randau, Jan Pané-Farré, Uwe Linne, Johannes Freitag, Rebecca Hinrichs, Stephan Kiontke, et al.The reaction was performed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, #E2040S) with the addition of 8 mM Ap4A (Jena Bioscience), 10 mM UTP and CTP, and 2 mM GTP. The reaction was

(7) Immune Checkpoint Inhibitor as a Fifth Component of Lipid Nanoparticles Enables Lymph Node-Targeting and Augments mRNA Vaccine EfficacyAdv Sci (Weinh)September 27, 2026Minki Ha, Jaehyun Kim, Seyoung Kim, Gonna Somu Naidu, Sungchan Cho, Seok-Beom Yongtemplate, COVID‐19 spike mRNA was produced with the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S) and components (5 mm ATP/CTP/GTP/N1‐methyl pseudoUTP, and 5 mm CleanCap Reagent AG (3’ OMe) [TriLink

(8) A Novel SINE Transposable Element-Associated lncRNA Regulates Zygotic Genome Activation in Porcine Parthenogenetically Activated EmbryosCellsSeptember 19, 2026Tianyao He, Shanlong Zhang, Shichao Zhang, Dongsong Liu, Han Li, Jun Song, Zhonghua Liu, Jun-Xue Jin, Jing-Tao Sunsequence were amplified by PCR and transcribed using the HiScribe™ T7 High Yield RNA Synthesis Kit (E2040S, NEB, Ipswich, MA, USA). Capped mRNAs were generated by co-transcriptional addition of Cap1 analog (NEB,

(9) Proximity biotinylation at the host-Shigella interface reveals UFMylation as an antibacterial pathwayNature CommunicationsSeptember 17, 2026Ana T. López-Jiménez, Fabien Théry, Kathryn Wright, Hannah Painter, Shelby T. Hoffmeister, Lucas Jarche, Jeremy Benjamin, Gerbrand J. van der Heden van Noort, Dominik Brokatzky, Margarida C. Gomes, et al.Qiagen, #28104). DNA templates were in vitro transcribed (HiScribe T7 High Yield RNA Synthesis Kit NEB, #E2040S) and purified (GeneJET RNA Cleanup and Concentration Micro Kit, ThermoFisher, #K0841). Gene expression analysis

(10) Host eicosanoid signals define a granuloma fibroblast population that coordinates mycobacterial containmentCell Host & MicrobeSeptember 9, 2026Erika J. Hughes, Charlie J. Pyle, Mark Chambers, Jana Travnickova, Thabo Mpotje, Jacob P. Lowy, Nathan T. Strang, Carson E. Carranza, Henry K.E. Ohman, Threnesan Naidoo, et al.NucleoSpin Gel and PCR Clean-up kit (Takara #740609) and transcribed using NEB T7 HiScribe kit (NEB #E2040S). RNA was DNase-treated, purified using Monarch Spin RNA Cleanup Kit (NEB #T2040L), diluted to 500 ng/μl

(11) RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted SystemAdv Sci (Weinh)August 21, 2026Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, et al.and Prnp, the in vitro transcription was carried out according to manufacturer's instructions (NEB, E2040S), with the following modifications. Reactions included 1 µg of DNA template and were supplemented with

(12) An integrated nanopore-microfluidic platform for low-voltage electroporation of delivering self-amplifying RNA into dendritic cellsMicrosyst NanoengAugust 17, 2026Bowen Zhang, Yijing Cai, Caiguanxi Deng, Xinshuo Huang, Chuanjie Yao, Xiaotong Li, Lukang Gao, Yujuan Wu, Jinkun Chen, Juan Jiang, et al.MluI (NEB, R3198) as the synthesis template. The RNA was synthesized by using T7 polymerase kit (NEB, E2040S) in cooperation with 2 mM N1-methylpseudouridine-triphosphate (APExBIO, B8049). The precursor RNA was

(13) Nuclear speckle-associated perispeckle networks partition active chromatin into gene expression nichesCell ReportsAugust 15, 2026Neha Chivukula Chivukula Venkata, Jiah Kim, Saireet Misra, Gabriel P. Faber, Atabek Bektash, Pankaj Chaturvedi, Gabriela Andrea Hernandez Hernandez Gonzalez, Joseph Dopie, Masato T. Kanemaki, Kyu Young Han, et al.by in-vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs cat# E2040S) and reverse-transcription using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific cat#

(14) Odorant receptor coexpression and multi-expression in the dengue mosquitoNature CommunicationsAugust 15, 2026Elisha David, Vitor L. dos Anjos, Sumer M. Kotb, Melanie Edwards, Hillery C. Metz, David Tian, Zhilei Zhao, Jessica L. Zung, Noah H. Rose, Carolyn S. McBrideHigh-Fidelity polymerase (NEB, M0541S). We then transcribed sgRNA in vitro using the HiScribe T7 Kit (NEB, E2040S) with an 8–9 h incubation at 37 °C. We purified the transcription products using RNAse-free SPRI beads

(15) Site-specific N6-methyladenosine fine tunes CTCF/cohesin-defined enhancer-promoter interactions and oncogenic transcription programResearch SquareAugust 14, 2026Huacheng Huang, Huacheng Hamamoto, Jingwen Hamamoto, Jingwen Song, Tsz Kan Fung, Songtao Lesperance, Julia Lesperance, MingJiang Li, Mingjiang Chen, Honglin Chen, et al.(Thermo Fisher Scientific #EP0113) or HiScribe T7 high-yield RNA synthesis kit (New England Biolabs #E2040S) according to the manufacturer’s instructions. 0.15nmol of in vitro transcribed RNAs were denatured

(16) Defective microhomology-mediated end joining in SMARCB1-deficient tumorsSci AdvAugust 14, 2026Guangli Zhu, Shuhei Asada, Huy Nguyen, Yuna Hirohashi, Lifang Sun, Avneesh Saravanapavan, Erfan Rahmani, Maryia Hrynashka, Sirisha Mukkavalli, Mona Batish, et al.in vitro–transcribed GFP mRNA generated using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, #E2040S) was added as a spike-in to 1 μg of cytoplasmic RNA and 1 μg of nuclear RNA, respectively, prior to

(17) Isothermal assay targeting the V1-V2 region of the 16S rRNA gene for rapid screening of Listeria spp. in raw milkInternational Dairy JournalAugust 11, 2026José E. Barboza-Corona, Sheila A. Martínez-Zavala, Angela P. Mendoza-Valerio, Cynthia P. Rangel-Chávez, Luz E. Casados-Vázquez, Drew Endywas used as a template. The RNA was synthesized and purified with kits from New England Biolabs (NEB, E2040S; T2050). LAMP assays were carried out using either a C1000 Touch thermal cycler (BioRad, Hercules, CA,

(18) DNA-bound avian R2 non-LTR retrotransposon protein recruits a second R2 protein for genome-protective second-strand nickingProc Natl Acad Sci U S AAugust 11, 2026Adedeji M. Aderounmu, Connor A. Horton, Briana Van Treeck, Anthony Rodríguez-Vargas, Kathleen Collinsdescribed methods (6). In brief, in vitro transcription used the HiScribe T7 High Yield Synthesis Kit (NEB, E2040S). Gel-purified RNA was aliquoted, flash frozen in liquid nitrogen and stored at −80 °C. DNA oligonucleotides

(19) Synthetic toehold biosensors for detecting Listeria monocytogenes in raw milkFront MicrobiolJuly 24, 2026Víctor M. Carballo-Uicab, Luz E. Casados-Vázquez, Drew Endy, José E. Barboza-Coronaamplicon was subjected to in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After 16 h (overnight) of incubation at 37 °C, the samples were treated with DNase I (RNase-free,

(20) Accurate, sensitive, and efficient chromatin accessibility quantification at target loci using UNIChro-seqNature CommunicationsJuly 20, 2026Michihiro Kono, Hiroaki Hatano, Kenichiro Asahara, Masahiro Nakano, Reza Bagherzadeh, Tsugumi Kawashima, Takahiro Arakawa, Miho Sato, Hajime Inokuchi, Takahiro Nishino, et al.(Addgene #178113 and #178114). mRNA was generated by IVT using a HiScribe T7 High-Yield RNA Kit (NEB, E2040S). All mRNA was produced with UTP fully replaced with N1-methylpseudouridine-5’-triphosphate (TriLink,

Write a Review

(1)

7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
(1)

4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

Citations (583)

Reviews

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map