HiScribe T7 High Yield RNA Synthesis Kit E2040S from New England Biolabs

Product Specs
ItemHiScribe T7 High Yield RNA Synthesis Kit
CompanyNew England Biolabs
Price $244.00 Inquire
Catalog NumberE2040S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Plant-associated fungi co-opt ancient antimicrobials for host manipulationSci AdvApril 29, 2026Fantin Mesny, Valentina Wolf, Ana López-Moral, Anton Kraege, Wilko Punt, Saifei Liu, Jinyi Zhu, Jiyeun Park, Yukiyo Sato, Bart P. H. J. Thomma(25 mM each), 1.5 μl of T7 buffer (10×), 1.5 μl of HiScribe T7 polymerase (New England Biolabs; ref. E2040S), 1 μl of dithiothreitol (0.1 M), and nuclease-free water up to 20 μl total volume. After incubation

(2) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay Yigitfollowing manufacturer’s recommendation at 37°C overnight, and template DNA was removed by DNase I (NEB, E2040S). The quality and purity of the resulting 1.57 kb RNA was assessed by gel electrophoresis, purified,

(3) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.concentrations of 0, 0.5, 1, 1.5, and 2% using the Hi-Scribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). IVT products were cleaned up using the Monarch Spin RNA Cleanup Kit (NEB, T2040L). mRNA concentration

(4) Lipid nanoparticle delivery of circle RNA vaccine induces potent immune responsesSci RepApril 2, 2026Ruohan Yang, Lin Jia, Jiuwei Cui(Sigma-Aldrich, F1804). circRNA was synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB E2040S). IVT templates were amplified via PCR before RNA synthesis (Q5 Hot Start High Fidelity 2x Master Mix).

(5) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.Mitochondria Isolation Kit for Cultured Cells,Thermo Fisher,89874 EasyPep kit,Thermo Fisher,A40006 HiScribe T7 RNA synthesis kit,NEB,E2040S Monarch RNA cleanup kit,NEB,T2040L DNA Clean & Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit

(6) Robust CD8+ T cell responses induced by an mRNA-LNP vaccine encoding rat HER2 extracellular domain confer prophylactic tumor protectionFront ImmunolApril 1, 2026Kexin Li, Xiaoya Li, Cong Zhang, Suli Dai, Lei Li, Jun Wang, Hongtao Zhang, Lianmei ZhaoThermo Scientific, USA) and served as templates for in vitro transcription employing T7 RNA polymerase (#E2040S, New England Biolabs, USA). During transcription, m1ψ-5’-triphosphate (#N-1081-10, TriLink, USA) was

(7) Synthetic circRNAs employ IRES activity for translation in cells and in cell-free translation systemsbioRxivMarch 30, 2026Philipp Koch, Frederic S. W. Arendrup, Chaehee Lim, Samyukta Narayanan, Amina Adam, Massimiliano Clamer, Anders H. Lund, Chun-Kan Chen, Kathrin Leppekdishes and transfected the following day with 1 μg of circRNA, generated by IVT (NEB HiScribe T7 kit, E2040S) and subsequently purified using RNase R (Biozym, 172010) digestion with or without prior 4% urea-PAGE

(8) A Biochemical Analysis of LINC00896 RNA in Cortex Neuronal Cells and Its Possible Connection to the Development of AutismJournal of Nucleic AcidsMarch 28, 2026Rachel A. Hellmann Whitaker, Joshua J. Storey, Isabella Vannavong, Robert S. Mangiamele, Micalyn A. Trihus, Donovan D. Floyd, Trevor Rohder, Dylan L. Cleveland, Andrew Rizeareproducibility. 2. Materials and Methods 2.1. Materials HiScribe T7 High Yield RNA Synthesis Kit E2040S (New England BioLabs, Ipswich, MA) was used to in vitro transcribe LINC00896 RNA via runoff transcription

(9) A dual-reporter mouse for therapeutic discovery in Angelman syndromeJCI InsightMarch 9, 2026Hanna Vihma, Lucas M. James, Hannah C. Nourie, Audrey L. Smith, Siyuan Liang, Carlee A. Friar, Tasmai Vulli, Lei Xing, Dale O. Cowley, Alain C. Burette, et al.[UNC] Animal Models Core), transcribed in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (E2040S, New England Biolabs), and purified via RNeasy spin columns (74104, QIAGEN) in microinjection buffer

(10) Cell-free DNA methylome and fragmentome analysis for relapse monitoring of Ewing sarcomaEMBO Molecular MedicineMarch 6, 2026Sophie A Richardson, Aram Safrastyan, Mina Karimpour, Gayatri Gulati, Patrick J B Harker, Alan Redfern, Simon P Pearce, Vsevolod J Makeev, Bernadette Brennan, Alexander T J Lee, et al.Sensifast SYBR Hi-ROX Kit,Bioline (SLS),BIO-92005 HiScribe T7 High Yield RNA Synthesis Kit,New England Biolabs,E2040S Agencourt AMPure XP,Beckman Coulter,A63881 FastAP Thermosensitive Phosphatase,Thermo Fisher,EF0654 "T4 RNA Ligase 2, Truncated KQ

(11) RNA-specific local translation is patterned by condensates for multinucleate cell growthNat Cell BiolMarch 2, 2026Zachary M. Geisterfer, Ameya P. Jalihal, Sierra J. Cole, Amy S. Gladfeltertranslation-competent Nluc or MoonTag mRNAs were carried out according to manufacturer’s specifications (NEB, E2080S or E2040S). The cDNA template was amplified using PCR for all Nluc constructs (primers given in Supplementary

(12) An in vitro transcription-based protocol for mapping HIV integration sites using lentiviral integration site sequencingSTAR ProtocolsMarch 1, 2026Kamil Więcek, Janusz Wiśniewski, Heng-Chang Chen16500500 T7 RNA polymerase mix (supplied in HiScribe T7 High Yield RNA Synthesis kit),New England Biolabs,E2040S 100 mM ATP (supplied in HiScribe T7 High Yield RNA Synthesis kit),New England Biolabs,E2040S 100 mM

(13) Diverse database and machine learning model to narrow the generalization gap in RNA structure predictionScience AdvancesFebruary 27, 2026Albéric A. de Lajarte, Yves J. Martin des des Taillades, Justin Aruda, Pierre Bongrand, Federico Fuchs Wightman, Dragui Salazar, Matthew F. Allan, Colin Kalicki, Casper L’Esperance-Kerckhoff, Alex Kashi, et al.in vitro transcription reaction using the HiScribe T7 High Yield RNA (New England Biolabs, catalog no. E2040S) kit according to the manufacturer’s instructions. Following DNase digestion (Invitrogen, catalog no.

(14) Scalable and multimodal brain angiogenesis and blood-brain barrier genetics by somatic mutagenesisCommunications BiologyFebruary 25, 2026Jay Mehul Panji, Raoul Freitas Vale Germano, Michelle America, Liang Lou, Laurence Lecordier, Patricia Tebabi, Maud Martin, Benoit Vanhollebekeas published previously15 using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, E2040S). The following sgRNA target sites were used: cldn5a_1: 5’-GCGCGGGTGGTGAACGTCGGAGG-3’, cldn5a_2: 5’-GGACAACGTGAAAGCGCGGGTGG-3’,

(15) Nuclear import of malaria RNA rewires splicing in host immune cellsCell ReportsFebruary 24, 2026Paula Abou Karam, Edo Kiper, Tamar Ziv, Shaked Yadid, Ewa Kozela, Nir Zharoni, Reinat Nevo, Daniel Alfandari, Helina Otesh, Abel Cruz Camacho, et al.mRNA Library Prep Kit,Illumina,N/A HiScribe T7 In Vitro Transcription Kit,New England Biolabs,Cat# E2040S HCR v3.0 smFISH assay (B1/B2/B3 amplifier system),Molecular Instruments,https://www.molecularinstruments.com ,, Deposited

(16) Restoration of Defective CFTR in Human Nasal Respiratory Epithelial Cells by CFTR Modulators and mRNA TransfectionInternational Journal of Molecular SciencesFebruary 23, 2026Roshani Narayan Singh, Marilia Marta Horn, Marin Juko, Ami Kampshoff, Jochen Schmid, Heymut Omran, Dandan Zhang, Joseph Rosenecker, Wolf-Michael Weber, Jörg Große-Onnebrinkto the CFTR open reading frame was synthesised using the HiScribe® T7 High Yield RNA Synthesis Kit (E2040S; New England, Biolabs, Ipswich, MA, USA), with co-transcriptional incorporation of a Cap 1 structure

(17) Comparison of Immune Cell Transfection by Different Vaccine Vectors After Intradermal InjectionVaccines (Basel)February 16, 2026Jiani Liu, Destin T. Hinson, Michael J. Hansen, Virginia P. Van Keulen, Brian J. Parrett, Larry R. Pease, Michael A. Barry[21,22]. This was used as a template for IVT using a T7 RNA synthesis kit (New England Biolabs HiScribe E2040S). The mRNA was synthesized with N1-Methylpseudouridine-5′-Triphosphate (m1ΨTP, Trilink N-1081) and co-transcriptionally

(18) Detection of Cancer-Associated Mutations Using Primer Exchange Reaction-Based Signal Amplification and Lateral Flow AssaysSmall SciFebruary 6, 2026Samet Kocabey, Curzio Rüeggdeoxynucleotide (dNTP) solution mix (N0447S), RNase H (M0297S), HiScribe T7 High Yield RNA Synthesis Kit (E2040S) and Monarch RNA Cleanup Kit (T2050L, 500 μg) were purchased from New England Biolabs (NEB) (Ipswich,

(19) TMPRSS2 is dispensable for lethal SARS-CoV-2 BA.5 infection in humanized hACE2 golden hamstersEuropean Journal of Medical ResearchJanuary 23, 2026Bowen Wang, Jinwei Zhang, Yuhan Li, Haodong Wang, Mei Dong, Qiu Chen, Aimin Shi, Aihua Zhang, Wentao Zeng, Xuefeng Wang, et al.producing sgRNAs via in vitro transcription using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). After treatment with lithium chloride precipitation to purify these sgRNAs, they were preserved at

(20) Dynamont: A comprehensive cross-species comparison of ONT segmentation toolsGigaScienceJanuary 21, 2026Jannes Spangenberg, Christian Höner zu zu Siederdissen, Winfried Goettsch, Lennart Köhler, Liz Maria Luke, Kai Papenfort, Manja Marz\begin{document} $\mu$\end{document}L IVT reactions for 1 hour using the HighScribe T7 RNA synthesis Kit (NEB–E2040S), following the manufacturer’s instructions. After IVT, the DNA templates were digested by addition

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(1)

7/23/2023RNA Synthesis Kit From NEB Ganesh PusapatiThe WNT pathway is essential for embryonic development and adult tissue homeostasis. Deregulation of this pathway results in cancers of the stomach, intestine, breast, etc. I am interested in studying... Read Review
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4/26/2022Great RNA Synthesis Kit Nan WangThis is high fidelity RNA synthesis kit from New England Biolabs. The synthesized RNA can be used for in vivo microinjection to achieve the RNAi effect.

HiScribe T7 High Yield RNA Synthesis Kit

HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

New England Biolabs

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HiScribe T7 High Yield RNA Synthesis Kit from New England Biolabs

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