Brilliant Violet 650™ anti-mouse CD4 100555 from BioLegend

Product Specs
ItemBrilliant Violet 650™ anti-mouse CD4
CompanyBioLegend
Price $273.00Supplier Page View Company Product Page
Catalog Number100555
Quantity50 µg
ApplicationsFC - Quality tested
Concentrationµg sizes: 0.2 mg/mLµL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Conjugate/TagBrilliant Violet 650™
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ReactivityMouse
IsotypeRat IgG2a, kappa
CloneRM4-5
HostRat
Antibody TypePrimary
ImmunogenBALB/c mouse thymocytes
NCBI Full Gene NameCD4 antigen
NCBI Gene AliasesL3T4, Ly-4
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Exercise suppresses breast cancer and reprograms the immune tumor microenvironment, cancer cell intrinsic features, and their interactionJournal of Sport and Health ScienceDecember 1, 2026Graeme J. Koelwyn, Sayyed Hamed Shahoei, Courtenay Graham, Geoffrey M. Nonis, Milad Vahedi, Puneet Sidhu, Emily J. Brown, Alireza Khodadadi-Jamayran, Juan Liu, Elisa De Stanchina, et al.BioLegend cat. no. 100330) and then further gated according to positive CD4 (BV650, BioLegend cat. no.100555) or CD8 (CD8a: FITC, eBioscience cat. no. 11-0081-82) status for helper and cytotoxic T cells respectively.

(2) Gut Microbiota Regulates Systemic Inflammatory Response and Compensatory Anti‐Inflammatory Response Syndromes by Targeting PF4 + Macrophages in Acute PancreatitisAdvanced ScienceMay 26, 2026Liwei Liu, Guanqun Li, Dongxu Lu, Haoran Ding, Tianqi Lu, Yuhang Sui, Can Zhang, Yu Xie, Rui Kong, Hua Chen, et al.Cat.123113), mouse Ly6g (BioLegend, Cat.127607), mouse CCR1 (BioLegend, Cat.152503), mouse CD4 (BioLegend, Cat.100555), human CD3 (BioLegend, Cat.317335), human CD4 (BioLegend, Cat.300517), human CD25 (BioLegend, Cat.356103),

(3) Genotoxicity profiling reveals distinct platform- and cell type–specific effects in therapeutic gene editing for genetic hyperinflammationCell Stem CellMay 13, 2026Lei Lei, Masako M. Kaufmann, Jessica Lao, Gudrun Thoulass, Sandra Ammann, Hui Xiao, Manuel Rhiel, Viviane Dettmer-Monaco, Julian Grünewald, Geoffroy Andrieux, et al.RRID:AB_1279222; clone HM34 CD4 APC-Cy7,Biolegend,Cat.#116020; clone RM4-5 CD4 BV650,Biolegend,Cat.#100555; RRID:AB_2562529; clone RM4-5 CD4 BV711,Biolegend,Cat.#100557; RRID:AB_2562607; clone RM4-5 CD44 APC,eBioscience,Cat.#17-0441-82;

(4) Identifying microbial protease allergens through protein language model-guided homologyCell SystemsMarch 18, 2026Kumar Thurimella, Elena Wu, Chenhao Li, Daniel B. Graham, Róisín M. Owens, Damian R. Plichta, Caroline L. Sokol, Ramnik J. Xavier, Sergio BacalladoClone: GK1.5",BioLegend,"Cat# 100412, RRID:AB_312697" "Anti-mouse CD4, Clone: RM4-5",BioLegend,"Cat# 100555, RRID:AB_2562529" "Anti-mouse CD4, Clone: RM4-5",BioLegend,"Cat# 100536, RRID:AB_493701" "Anti-mouse

(5) Integrating Mathematical and Mouse Models Identifies T Regulatory Cell Influx as A Key Determinant of Acquired Resistance to PD-1 ImmunotherapybioRxivJanuary 16, 2026Rachel S Sousa, Shannon N Geels, Claire Murat, Alexander Moshensky, S Armando Villalta, John S Lowengrub, Francesco Marangoni145-2C11),BioLegend,Cat# 100340 CD4 BV605 (clone: RM4-5),BioLegend,Cat # 100548 CD4 BV650 (clone: RM4-5),BioLegend,Cat # 100555 CD45 BV510 (clone: 30-F11),BioLegend,Cat # 103138 CD45.1 PE-Cy (clone: A20),BioLegend,Cat # 110729 CD45.2

(6) Loss of E3 ligase Ube4A Disrupts Colon Homeostasis and Accelerates Experimental Colitis via Altered Lipid HandlingbioRxivJanuary 6, 2026Simon Guignard, Molee Chakraborty, Silvia Gonzalez-Nieves, David DeBruin, Emily Ebert, Anastasiia Vinogradskaia, Michelle Brennan, Ryan M Teague, Anutosh Chakraborty, Vincenza CifarelliBiolegend (anti-CD45, cat. #157612, clone QA17A26; anti-CD8a, cat. #100750, clone 53–6.7; anti-CD4, cat. #100555, clone RM4–5; anti-CD19, cat. #115546, clone 6D5; anti-CD11b, cat. #101222, clone M1/70; anti-NK1.1,

(7) High Fat and Sugar Diet Increases Enteric cDC1 and Oral Antigen-Specific TregsJ Immunol ResOctober 28, 2025K. Fischer, O. Horno, B. Raposo, I. Godinho, H. Ribeiro, H. Veiga-Fernandes, M. Martínez-LópezBiotin,BioLegend,100304 Anti‐mouse CD4 BV785,BioLegend,100551 Anti‐mouse CD4 BV421,BioLegend,100437 Anti‐mouse CD4 BV650,BioLegend,100555 Anti‐mouse CD40 PerCP Cy‐5.5,BioLegend,124623 Anti‐mouse CD43 Biotin,eBioscience,13‐0341‐82 Anti‐mouse

(8) Progressive changes in phenotype, transcriptome and proliferation capacity characterise continued maturation and migration of intestinal cDCs in homeostasisNature CommunicationsSeptember 2, 2025Fabian T. Hager, Trong Hieu Nguyen, Asmae Laouina, Lydia Kopplin, Anna Andrusaite, Susan A. V. Jennings, Britta Simons, Andrea Leufgen, Thomas Clavel, Simon Milling, et al.121410), CD11b (M1/70, 101243), CD11c (N418, 117318), CD19 (6D5, 115520), CD3 (17A2, 100236), CD4 (RM4-5, 100555), CD40 (3/23 124610), CD45 (30-F11 103105, 103114), CD45.2 (104, 109806, 109808), CD62L (MEL-14, 104445),

(9) Combinatorial treatment with upadacitinib abrogates systemic toxicity of a tumor-targeted IL-2 fusion proteinJ Immunother CancerMay 11, 2025Giulia Rotta, Eleonora Prodi, Frauke Seehusen, Matilde Bocci, Francesco Prisco, Ettore Gilardoni, Claudia Comacchio, Cornelia Halin, Emanuele Puca, Dario Neri, et al.anti-CD8-BV605 (1:200, BioLegend Cat#100743, RRID: AB_ 2561352), anti-CD4-BV650 (1:200, BioLegend Cat# 100545, RRID: AB_11126142), anti-NKp46-PECy7 (1:200, BioLegend Cat# 137617, RRID: AB_11218594), anti-CD44-APC

(10) Gluten-Free Diet Induces Small-Scale Changes Across Multiple T-Cell Subsets in NOD MiceEur J ImmunolApril 21, 2025Veronika Niederlova, Juraj Michalik, Barbora Drabonova, Radka Cisarova, David Funda, Ondrej Stepanek(https://github.com/Lab‐of‐Adaptive‐Immunity/NOD_scRNAseq). 5.4 Antibodies and Reagents 5.4.1 Extracellular Markers Anti‐CD4 BV650 (clone RM4‐5, Biolegend #100545) or AF700 (clone RM4‐5, Biolegend #100536), anti‐CD8a FITC (clone 53–6.7, eBioscience #11‐0081‐81) or

(11) Statin therapy associated Lactobacillus intestinalis attenuates pancreatic fibrosis through remodeling intestinal homeostasisNPJ Biofilms MicrobiomesApril 15, 2025Yuhang Sui, Tao Zhang, Suwen Ou, Guanqun Li, Liwei Liu, Tianqi Lu, Can Zhang, Yukai Cao, Rui Bai, Haoxin Zhou, et al.(Biolegend, 103132), anti-CD3 (Biolegend, 100234), anti-CD8a (Biolegend, 100714), anti-CD4 (Biolegend, 100555), anti-CD11b (Biolegend, 101217), anti-Ly6G (Biolegend, 127607), anti-F4/80 (Biolegend, 123141) and

(12) Developmental epigenetic programming by Tet1/3 determines peripheral CD8 T cell fateEMBO ReportsApril 2, 2025Kara M Misel-Wuchter, Andrew L Thurman, Jordan T Johnson, Athmane Teghanemt, Neelam Gautam, Alejandro A Pezzulo, Jennifer R Bermick, Noah S Butler, Priya D Issuree(clone A20),Tonbo,"Cat#: 70-0453, RRID:AB_2621497" BV650 anti-mouse CD4 (clone RM4-5),Biolegend,"Cat#: 100555, RRID:AB_2562098" FITC anti-mouse CD8a (clone 53-6.7),Biolegend,"Cat#: 100706, RRID:AB_312745" BV650

(13) Immune targeting and host-protective effects of the latent stage of Toxoplasma gondiiNature MicrobiologyMarch 27, 2025Julia N. Eberhard, Lindsey A. Shallberg, Aaron Winn, Sambamurthy Chandrasekaran, Christopher J. Giuliano, Emily F. Merritt, Elinor Willis, Christoph Konradt, David A. Christian, Daniel L. Aldridge, et al.BUV496, BD Biosciences: 612952, clone: GK1.5, RRID:AB_2813886, dilution 1:200; CD4: BV650, Biolegend: 100555, clone: RM4-5, RRID:AB_2562529, dilution 1:400; CD4: FITC, eBioscience: 11-0041-85, clone: GK1.5, RRID:AB_464892,

(14) Aspirin prevents metastasis by limiting platelet TXA2 suppression of T cell immunityNatureMarch 5, 2025Jie Yang, Yumi Yamashita-Kanemaru, Benjamin I. Morris, Annalisa Contursi, Daniel Trajkovski, Jingru Xu, Ilinca Patrascan, Jayme Benson, Alexander C. Evans, Alberto G. Conti, et al.143808, 1/400), anti-CD4-AF700 (RM4-5, BioLegend, 100536, 1/200), anti-CD4-BV650 (RM4-5, BioLegend, 100546, 1/400), anti-CD44-BV510 (IM7, BioLegend, 103044, 1/200), anti-CD44-BV786 (IM7, BD, 563736, 1/400),

(15) Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasisCell ReportsFebruary 25, 2025Phuong T.T. Nguyen, Mia Shiue, Nina Kuprasertkul, Pedro Costa-Pinheiro, Luke T. Izzo, Laura V. Pinheiro, Hayley A. Affronti, Gabriel Gugiu, Shivani Ghaisas, Joyce Y. Liu, et al.(Cell Signaling Technology, 18452) in PBS 15 minutes at 4°C, then with Brilliant Violet 650™ anti-mouse CD4 (BioLegend, 100555), PE/Cyanine7 anti-mouse CD8α (BioLegend, 100722), and Alexa Fluor® 700 anti-mouse CD45 (BioLegend,

(16) Splenic fibroblasts control marginal zone B cell movement and function via two distinct Notch2-dependent regulatory programsImmunityJanuary 14, 2025Anneka Allman, Brian T. Gaudette, Samantha Kelly, Nagham Alouche, Léolène J. Carrington, Eric Perkey, Joshua D. Brandstadter, Riley Outen, Ashley Vanderbeck, Katlyn Lederer, et al.1B1)",BD Biosciences,Cat# 740366; RRID:AB_2740098 "anti-mouse CD4 BV650, Clone GK1.5",BioLegend,Cat# 100555; RRID:AB_2562529 "anti-mouse CD19 BV785, Clone 6D5",BioLegend,Cat# 115543; RRID:AB_11218994 "anti-mouse

(17) Fungal symbiont transmitted by free-living mice promotes type 2 immunityNatureDecember 19, 2024Yun Liao, Iris H. Gao, Takato Kusakabe, Woan-Yu Lin, Alexander Grier, Xiangyu Pan, Olga Morzhanaeva, Terrance P. Shea, Hiroshi Yano, Danielle Karo-Atar, et al.1:200), anti-TCRβ (clone H57-597) (Cat# 109227, Biolegend, 1:200), anti-CD4 (clone RM4-5) (Cat# 100548, 100545, Biolegend, 1:200), anti-CD8 (clone 53-6.7) (Cat# 100730, Biolegend, 1:200; Cat# 563786, BD Biosciences,

(18) An IL-2 mutein increases regulatory T cell suppression of dendritic cells via IL-10 and CTLA-4 to promote T cell anergyCell ReportsNovember 26, 2024Braxton L. Jamison, Matthew Lawrance, Chun Jing Wang, Hannah A. DeBerg, Lauren J. Ziegler, David M. Sansom, Marc A. Gavin, Lucy S.K. Walker, Daniel J. CampbellRESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Anti-mouse CD4 BV650 (RM4-5),BioLegend,Cat# 100555; RRID: AB_2562529 Anti-mouse CD4 BV421 (RM4-5),BioLegend,Cat# 100563; RRID:AB_2563052 Anti-mouse CD45

(19) Computationally designed mRNA-launched protein nanoparticle vaccinesbioRxivJuly 25, 2024Grace G. Hendricks, Lilit Grigoryan, Mary Jane Navarro, Nicholas J. Catanzaro, Miranda L. Hubbard, John M. Powers, Melissa Mattocks, Catherine Treichel, Alexandra C. Walls, Jimin Lee, et al.cocktail containing anti-CD3 (BV785; BioLegend #100355, 1:50 dilution), anti-CD4 (BV650; BioLegend #100555; 1:200 dilution), anti-CD8 (BV711; BD #563046; 1:200 dilution), anti-CD45 (BUV395, BD # 564279). Cells

(20) A RIPK1-specific PROTAC degrader achieves potent antitumor activity by enhancing immunogenic cell deathImmunityJuly 9, 2024Mannion J, Gifford V, Bellenie B, Fernando W, Ramos Garcia L, Wilson R, John SW, Udainiya S, Patin EC, Tiu C, et al.Clone#H57–597 α-mouse CD8α-BUV395,BD Biosciences,Cat#563786; Clone#53–6.7 α-mouse CD4-BV650,Biolegend,Cat#100555; Clone#RM4–5 α-mouse CD44-BV785,Biolegend,Cat#103059; Clone#IM7 α-mouse CD25-EF450,Thermo Fisher Scientific,Cat#48–0251-82;

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(1)

4/11/2023Good CD4 Antibody for Multiple-Color Flow Panel Yao WangWe keep using this Cd4 antibody as our lineage marker to gate the Cd4 T cells in single cells from spleen, lymph nodes, and tumor samples.
(1)

5/20/2020Brilliant Violet 650™ Anti-Mouse CD4 Antibody Christoforos DimitropoulosThe antibody identified the CD4+ population in the brain of BL6 mice with high specificity and resolution.
(1)

12/3/2018Good Mouse CD4 Antibody Rebecca GuyerOur lab studies the function of T cells in an influenza model in mice. We need a reliable CD4 antibody that has good separation so that we can identify the T cells. This antibody works well ... Read Review
(1)

12/3/2018Mouse CD4 BV650 Christina StehleStaining of mouse lymphocytes using anti-mouse CD4 Brilliant Violet 650 clone RM4-5 from BioLegend. The staining is bimodal and very bright.
(1)

2/15/2017Great CD4 Antibody Maria Carmela SperanzaSplenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45, CD3 and CD4. The experiment was perform to evaluate the changes in CD4 population after treatment.

Brilliant Violet 650™ anti-mouse CD4 from BioLegend

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Brilliant Violet 650™ anti-mouse CD4 from BioLegend

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