Good Mouse CD4 Antibody

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-mouse CD4 BV650

Catalog Number:

100545

Our lab studies the function of T cells in an influenza model in mice. We need a reliable CD4 antibody that has good separation so that we can identify the T cells. This antibody works well consistently in our samples.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

Cells were stained in 100 µL FACS buffer with antibody at 1:100 for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 µL FACS buffer.

Blocking Agent

FACS buffer

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSR II

Results Summary

This antibody was bright enough to clearly stain the CD4+ population from our sample.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

NONE

The Bottom Line

Great antibody to identify CD4+ mouse cells.

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