Biolegend
Brilliant Violet 650™ anti-mouse CD4 Antibody
100555
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Splenocytes extracted from brain tumor-bearing mice, gated for live cells, CD45, CD3 and CD4. The experiment was perform to evaluate the changes in CD4 population after treatment.
Flow Cytometry
Mouse Splenocytes
30min
Fc Ab
N/A
Flow - Fortessa
After cell extraction and blocking with Fc Block, wash your sample 2x with washing buffer, stain for 30min RT with CD4 1:100, wash 2x, fix in PFA2% or proceed with intracellular staining protocol. Good antibody, tested on splenocytes, LN and TILs.
Clean population
None
Good antibody to detect a CD4