Mouse CD4 BV650

December 03, 2018

Innate Immunity Department
German Rheumatism Research Center Berlin
Ph.D. Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 650 anti-mouse CD4 Antibody

Catalog Number:

100545

I am interested in innate lymphoid cells of different organs and thus need antibodies to identify populations. Staining of mouse lymphocytes using anti-mouse CD4 Brilliant Violet 650 clone RM4-5 from BioLegend is bimodal and very bright.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung lymphocytes

Primary Incubation

CD4 BV650, clone RM4-5, 15 min at 37°C in PBS/BSA

Blocking Agent

Anti FcgR, clone 2.4G

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Symphony

Results Summary

Very bright staining on CD3+ T cells in C57BL/6 mouse lung.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and bimodal staining

The Bad

Nothing bad to say

The Bottom Line

This antibody gives reliable and stable results.

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