Use Of CD31 (PECAM-1) Antibody From R&D Systems On Differentiated Embryonic Stem Cell Culture

November 30, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D systems

Product Name:

Polyclonal CD31/PECAM-1 Antibody

Catalog Number:

AF3628

This antibody was used to analyze the presence of PECAM-1 positive endothelial cells in differentiated culture of mouse embryonic stem cells (mESCs). This polyclonal (goat) anti-CD31 antibody appears specific to endothelial tissue, with a cell membrane distribution, as expected for a CAM.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse embryonic stem cells (mESCs), differentiated. Fixed Adherent culture.

Primary Incubation

1:200, overnight, at 4C in Blocking agent, on shaker

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-488-conjugated Donkey anti-GOAT IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on shaker

Tertiary Incubation

N/A

Detection

Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection

Results Summary

This antibody works well on fixed adherent culture containing endothelial cells. Cell membrane distribution.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal to endothelial cell by immunofluorescence

The Bad

not known

The Bottom Line

Good antibody overall. Recommended for immunofluorescence study of endothelial cells in mouse cell cultures. This antibody reacts with the cell adhesion molecule PECAM-1, also known as CD31. The antibody is a Goat polyclonal, care should be taken to use Donkey secondary antibodies when doing co-staining studies.

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