CD31 Antibody for Immunofluorescence

February 08, 2022

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

R&D

Product Name:

CD31 antibody

Catalog Number:

AF3628

Clone Number:

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Excellent antibody for marking the vasculature in tissue, sections, and slices.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

E17 mouse brain slices

Primary Incubation

1:200, 4° C overnight

Blocking Agent

10% NDS in PBST (0.5% TritonX 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Great antibody for staining the vasculature in embryonic, postnatal or adult sections.

DOI or PMID #

Additional Notes

Can work better with Antigen retrieval (10mM Sodium citrate-boiling)

Image Gallery

Summary

The Good

Specific staining, works even for thicker sections upto 100µm , no background

The Bad

None

The Bottom Line

Highly recommended

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