Great CD31 Antibody for IF

May 02, 2022

Institute of Microbiology, Infectious Diseases and Immunology
Charité – Universitätsmedizin Berlin
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

CD31

Catalog Number:

AF3628

Clone Number:

n/a

Info:Supplier Page View Product Specs

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We are interested to study tissue-resident macrophages and their interplay with endothelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PLP fixed 18 μm cryosections of mouse liver from a 10 week old C57BL/6J mouse

Primary Incubation

1:400 overnight at room temperature

Blocking Agent

1% BSA, 1% FCS, 0.3% Triton X, 2% mouse serum, 2% donkey serum

Secondary Incubation

1:500 donkey anti-goat alexa fluor 555 1h at room temperature

Tertiary Incubation

N/A

Detection

Images were acquired using an inverted Zeiss LSM780 confocal microscope with an 20x 0.8NA air objective.

Results Summary

Great staining of endothelial cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Amazing staining.

The Bad

None

The Bottom Line

According to my experience, the best CD31 antibody I tested so far.

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