Fig 1: Single-cell RNA-seq of non-cardiomyocytes in the murine heart.Related to Fig. 1. a: t-distributed stochastic neighbour embedding (t-SNE) visualisation of unsupervised clustering of non-cardiomyocytes. The cells (dots) are coloured according to the cell clusters, and the numbers represent cluster numbers. b: Non-cardiomyocytes classification based on gene expression using t-SNE. Non-cardiomyocytes were defined as cells expressing each marker gene. Marker genes include Car4 (capillary endothelium), Cd14 (macrophage), Acta2 (smooth muscle cells), Efnb2 (arterial endothelium), Cd80 (dendritic cells), Vwf (vein endothelium), Mki67 (Mki67 positive endothelium), Plp1 (nerves), Mmrn1 (lymphatic endothelium), Cd79a (B cells), and Cd3g (T cells). c: Violin plot depicting HF-Fibro (cluster 4) expression of Postn but not Acta2 or Chad. d: Violin plot showing the expression of transcription factors in six clusters. e: FACS analyses of PDGFRα and MYC expression in sham (TAC0w), TAC4w, TAC6w, and TAC 8w hearts.