CD3 Antibody From BioLegend Yields Clear CD3+ Populations by Flow Cytometry

Immunology
George Washington University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

FITC anti-mouse CD3 Antibody

Catalog Number:

100204

Clone Number:

17A2

I used this antibody to define CD3+ populations within my mouse leukocytes. This antibody worked as expected to provide distinct populations which I could quantify.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Mouse primary leukocytes

Primary Incubation

1:100

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Using this antibody, I was able to gate my CD3+ population among the leukocytes in my mouse samples. I compared these CD3+ populations across my treatment groups in order to make conclusions about the percentage of T cells in different samples.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Affordable and provides clear signal.

The Bad

None.

The Bottom Line

This antibody is affordable, works well, and requires very little optimization. I will continue to use this in my flow cytometry panel for mouse primary cells.

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