Anti-CD3 Stains T Cells Very Well

May 30, 2018

BlueskyReddit
Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-mouse CD3 Antibody

Catalog Number:

100204

We wanted to investigate T cells in the healthy vs diseased state, so we stained for CD3 to identify T cells in the mouse blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse blood

Primary Incubation

1:100 in flow staining buffer

Blocking Agent

1:100 FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD Fortessa flow cytometer

Results Summary

The antibody identifies T cells very well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Stains CD3+ cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying T cells

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