Nice T-cell Staining with Good Population Resolution

February 03, 2020

BlueskyReddit
Surgery
NYU Langone Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD3 Antibody

Catalog Number:

100204

Clone Number:

17A2

Great color and clone for easy staining of T cell population. The antibody is uncomplicated and has good enough resolution.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine pancreas with orthotopically injected murine pancreas cancer cells

Primary Incubation

Incubated at 4C for 1h, dilution 1:500

Blocking Agent

1% FACS, anti-CD16/CD32, Mouse serum, Rat IgG

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT

Results Summary

Straightforward staining at vendor recommended dilution and good identification of T cell population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy use

The Bad

Resolution adequate but could be better

The Bottom Line

Great CD3 antibody for uncomplicated use.

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