Small molecules are a broad class of low molecular weight compounds that are bioactive or produced from metabolic processes. Certain small molecules are noteworthy for their biological functions ranging from protein activation, enzyme inhibition, cellular metabolism, and signal transduction. Many small molecules are used as biomarkers and diagnostic agents to characterize a particular biological process or assess dysfunction. They are also often used in high-throughput screening assays to identify potential therapeutic agents. This catalog lists a variety of assay kits for common biologically relevant small molecules across different reagent suppliers. Such assay kits, which contain the necessary reagents in a ready-to-use format, can serve as useful tools for fast, convenient, and reproducible measurements of biological samples.
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Best practices for performing these types of experiments
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I want to investigate the genetic connection between cell wall hydrolase knockouts of bacteria and cell wall-acting antibiotic vancomycin. Therefore, I have used this product to examine the vancomycin sensitivity of these mutant bacteria.
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In my lab, I use the Pierce BCA Protein Assay Kit to measure protein concentrations in whole-cell extracts and, occasionally, purified proteins.
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