MojoSort™ Mouse CD8 T Cell Isolation Kit 480008 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price $483.00Supplier Page View Company Product Page
Catalog Number480008
Quantity100 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

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Website: biolegend.com

(1) High-titer modular retroviral vectors enabled by an antisense cassette design preventing dsRNA formation during virus productionMolecular Therapy AdvancesJune 11, 2026Romain Vuillefroy de Silly, Patrick Reichenbach, Melita Irvingcells were purified by negative selection using the Biolegend MojoSort mouse CD8+ T cell isolation kit (480008; >90% purity) and were primed by culture on plates pre-coated with anti-CD3ε antibodies (5 μg/mL; clone

(2) IL-12-secreting CAR-T cells reprogram the tumor microenvironment and improve efficacy against heterogeneous models of glioblastomaJ Immunother CancerMarch 24, 2026Steven Shen, Aditya A Mohan, Kelly M Hotchkiss, Sarah Cook, Kisha Patel, Eliese Moelker, Bhairavy Puviindran, Ariel T Gonzalez, Saif Zaidi, Samantha Spellicy, et al.suspensions were prepared, and total CD3+ T cells were isolated using a negative selection kit (BioLegend, #480007). The day prior to co-culture, 2×10⁶ CT-2A cells were seeded in T75 flasks with 100 IU/mL murine IFN-γ

(3) Antigen-specific immunotherapy with a CD4+ T cell neoepitope restrains CD8+ T cell differentiation in murine pancreatic islet graftsNature CommunicationsMarch 24, 2026James E. DiLisio, K. Scott Beard, Tobias Neef, Stephen D. Miller, Janet M. Wenzlau, Maki Nakayama, Marc A. D’Antonio, Braxton L. Jamison, Kelli S. Nicholson, Rocky L. Baker, et al.Tg/NY8.3 T cells to remove residual peptide. Tg/NY8.3 were isolated by CD8 negative selection (Biolegend #480008) and stimulated as Tg/BDC-2.5 T cells with the addition of recombinant murine IL7 (PeproTech # 217-17-50UG)

(4) ULK1 drives NDP52-mediated selective autophagic degradation of MHC-I to promote immune evasion in HPV-positive head and neck cancerbioRxivMarch 20, 2026Lexi Vu, Nicholas S. Giacobbi, Mohamed I. Khalil, Canchai Yang, William J. Eckerman, Evelyn Gomez Recinos, Johnathon D. Garber, Hyomin Son, Prerna Chahal, Dara M. Villa, et al.were isolated from 1×108 splenocytes using the MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend #480008) according to the manufacturer’s instructions. Isolated CD8+ T cells were cultured for 5 days in RPMI

(5) Context‐Dependent Role of GDF15: GDF15 + Tumor‐Associated Macrophages Suppress OSCC Progression by Enhancing PhagocytosisAdvanced ScienceMarch 13, 2026Xinyu Zhou, Zhihang Zhou, Houyu Ju, Chaoyang Guo, Fangxing Zhu, Yiyi Zhang, Xu Chen, Tongchao Zhao, Laiping Zhong, Jingzhou HuCD8+ T cells proliferation was evaluated by flow cytometry. MojoSort mouse CD8+ T cells isolation kit (480008, Biolegend, USA) was used for the isolation of CD8+ T cells according to the manufacturer's instruction.

(6) Oligodendrocyte transcription factor 2 orchestrates glioblastoma immune evasion by suppressing CXCL10 and CD8+ T cell activationJournal of Clinical InvestigationMarch 2, 2026Xinchun Zhang, Jinjiang Xue, Cunyan Zhao, Chenqiuyue Zeng, Jiacheng Zhong, Gangfeng Yu, Xi Yang, Yao Ling, Dazhen Li, Jiaxiao Yang, et al.cell strainer. After red blood cell lysis, CD8+ T cells were purified by CD8 microbeads (BioLegend, 480007) according to the manufacturer’s protocol. CD8+ T cells were stimulated with 3 μg/mL anti-CD3 and 1

(7) CD38 degrades MAVS through mitophagy to inhibit type I interferon secretion in nasopharyngeal carcinoma cells and impairs CD8+T cell-mediated anti-tumor immunityNature CommunicationsFebruary 9, 2026Lin Liang, Wentao Li, Siyi Liu, Qian He, Feng Zeng, Jiaying Cao, Yan Lei, Yanling Li, Yanhong Zhoumice (Cyagen Biosciences Inc., China) using the MojoSort Mouse CD8 T Cell Isolation Kit (Catalog No. 480007, BioLegend) according to the manufacturer’s instructions. The isolated OT-I CD8+T cells were activated

(8) SLC2A3‐Mediated Lactate Metabolism Promotes Lung Cancer Bone Metastasis by Modulating P53 Lactylation and Immune EvasionAdvanced ScienceFebruary 4, 2026Yi Ding, Yuying Tian, Wenjie Ren, Xianglin Hu, Mengjuan Li, Bei Liu, Chen chen, Yunhan Lu, Lei Li, Wangjun Yan, et al.Stimulation Peripheral CD8+ T cells were isolated from mouse spleens by MojoSort Mouse CD8+ T Cell Isolation Kit (480008, BioLegend). The cells were then stimulated with plate‐bound anti‐CD3 (1 µg/mL) (cat. no. 567115, BD

(9) Oligodendrocyte transcription factor 2 orchestrates glioblastoma immune evasion by suppressing CXCL10 and CD8+ T cell activationJ Clin InvestJanuary 27, 2026Xinchun Zhang, Jinjiang Xue, Cunyan Zhao, Chenqiuyue Zeng, Jiacheng Zhong, Gangfeng Yu, Xi Yang, Yao Ling, Dazhen Li, Jiaxiao Yang, et al.cell strainer. After red blood cell lysis, CD8+ T cells were purified by CD8 microbeads (BioLegend, 480007) according to the manufacturer’s protocol. CD8+ T cells were stimulated with 3 μg/mL anti-CD3 and 1

(10) IL4I1⁺ Macrophages and TDO2⁺ Myofibroblasts Drive AhR‐Mediated Immunosuppression and Ferroptosis Resistance in Solid Predominant Lung AdenocarcinomaAdvanced ScienceDecember 22, 2025Zhaoxuan Wang, Weijiao Xu, Lei Zhao, Lin Zhong, Wendan Yu, Shengmin Wang, Lu Sun, Tao Guo, Fengzhou Li, Zhuoshi Li, et al.the spleen of 6‐ to 8‐week‐old male C57BL/6 mice using the MojoSort Mouse CD8 T Cell Isolation Kit (480008, BioLegend) following the manufacturer's instructions. Mouse T‐Activator CD3/CD28 for T cell expansion

(11) Rroid2 regulates effector-to-memory CD8 + T cell differentiation during infection in vivoProceedings of the National Academy of SciencesDecember 2, 2025Julia Erber, Carmen Stecher, Valerie Plajer, Nina Braun, William Mallard, Loyal A. Goff, Iros Barozzi, Thomas Mohr, John L. Rinn, Richard A. Flavell, et al.from the spleen, enriched for CD8+ T cells using the MojoSort Mouse T Cell Isolation Kit (BioLegend, #480008) and then sorted into 700 µL RTL Plus lysis buffer supplemented with β-mercaptoethanol. RNA isolation

(12) Biomimetic carbon nanopolymers ANM-NPs act on mannose receptors and complement receptors to promote tumor antigen presentationJ Immunother CancerDecember 1, 2025Zhuangzhuang Feng, Xinxiu Shi, Zhiyang Li, Minming Lou, Bijia Cui, Mengzhan Chen, Lei Zhang, Yujie Sun, Heng Zhang, Yi-Nan Li, et al.purified from the spleen of 7-week-old C57BL/6 mice using the CD8+ T Cell Isolation Kit (BioLegend, 480008). After DCs maturation, DCs and T cells were co-cultured at a 1:10 ratio at 106 T cells/mL in RPMI-1640

(13) Murine T-cell receptor OT-I exhibits imperfect discrimination between foreign and self-antigensThe EMBO JournalNovember 26, 2025Anna Huhn, Mikhail A Kutuzov, Keir Maclean, Lion F K Uhl, Jagdish M Mahale, Audrey Gérard, P Anton van der Merwe, Omer DushekSelection was carried out with a MojoSortTM CD8 + T-cell negative isolation kit and magnets (Biolegend, #480008 and #480019). Isolated OT-I T cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076]

(14) RTP4 Suppresses Colorectal Cancer Progression via MHC-I-Mediated CD8(+) T Cell Infiltration and Enhances Immunotherapy ResponseJournal of cellular and molecular medicineOctober 1, 2025Yu C, Li Y, Liu Z, Ye W, Wei L, Xu W, Yan L, Ye H.OT‐I T cells were magnetically isolated using the MojoSor Mouse CD8+ T Cell Isolation Kit (Biolegend, 480008) from OT‐I mice according to the manufacturer's protocol. OT‐I T cells were then co‐cultured with vector

(15) USP14-mediated metabolic competition impairs CD8+ T cell immunosurveillance in hepatocellular carcinomaProceedings of the National Academy of SciencesSeptember 23, 2025Yichuan Yuan, Yi Niu, Zhenkun Huang, Yunxing Shi, Zhu Lin, Dinglan Zuo, Chengrui Zhong, Kai Li, Xin-Yuan Guan, Yunfei Yuan, et al.mouse (RRID: IMSR_JAX:003831) spleens using the MojoSort Mouse CD8 T Cell Isolation kit (Biolegend Cat# 480007) and activated using anti-mouse CD3 (Bio X Cell Cat# BE0001-1, RRID: AB_1107634) and anti-mouse CD28

(16) Protocol for analyzing T cell and macrophage motility in a mouse pancreatic cancer spheroid model using light-sheet microscopySTAR ProtocolsSeptember 1, 2025Vanessa Zimmer, Benedikt Slusny, Katrin Roth, Christian Bauerthe commercial magnetic bead-based and negative selection MojoSort Mouse CD8 T cell Isolation kit (# 480008) from BioLegend. Alternative kits for T cell enrichment and isolation are also possible. If you use

(17) Upstream open reading frame translation enhances immunogenic peptide presentation in mitotically arrested cancer cellsNature communicationsAugust 27, 2025Kowar A, Becker JP, Del Pizzo R, Tang Z, Champagne J, Wellach K, Samimi K, Galindo-Albarrán A, Körner PR, Montenegro Navarro J, et al.naive CD8 + T OT-I cells were isolated using the MojoSort Mouse CD8 T cell isolation kit (BioLegend, 480007) and subsequently activated for 72 h on plates coated with 2 μg/ml anti-CD3 (BioXCell, BE0001-1) and

(18) IFNγ-inducible Gbp4 and Irgb6 contribute to experimental cerebral malaria pathology in the olfactory bulbmBioAugust 13, 2025Matsuo-Dapaah J, Alshaweesh J, Lee MSJ, Hayashi T, Dash R, Kuroda M, Tainaka K, Ozawa M, Kuratani A, Yamamoto M, et al.Laboratory, Bar Harbor, ME) and enriched using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend; Cat# 480008). OT-I CD8+ T cells were then co-cultured with peptide-loaded BMDCs at a 1:1 ratio for 72 h. Following

(19) Gamma-retrovirus design for efficacious microRNA-mediated gene knock-down and protein co-expression without compromising titersNAR Molecular MedicineAugust 12, 2025Romain Vuillefroy de Silly, Bili Seijo, Jimmy Maillard, Patrick Reichenbach, Melita Irvingcells were purified by negative selection using the Biolegend MojoSort mouse CD8+ T cell isolation kit (480008; >90% purity) and were primed by culture on plates pre-coated with anti-CD3 antibodies (5 μg/ml; clone

(20) Universal Fab-Fc masked cytokine prodrug platform: αPD-L1/IL-15 prodrug activates CD44+ CD8+ T cells in tumor-draining lymph nodes to enhance antitumor immunityJournal for ImmunoTherapy of CancerJuly 31, 2025Luyao Song, Gen Qi, Wenqiang Shi, Qiongya Zeng, Ying Qin, Zejian Wang, Wei Li, Zilan Song, Ao Zhang, Huili LuBio-Medical Electronics, Cat#105-000443-00), MojoSortTM Mouse CD8 T Cell Isolation Kit (BioLegend, Cat#480008), MojoSortTM Streptavidin Nanobeads (BioLegend, Cat#480015), Cell Counting Kit-8 (Donjindo, Cat#CK04). Cell

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(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.

MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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