MojoSort™ Mouse CD8 T Cell Isolation Kit 480008 from BioLegend

Product Specs
ItemMojoSort™ Mouse CD8 T Cell Isolation Kit
CompanyBioLegend
Price $483.00Supplier Page View Company Product Page
Catalog Number480008
Quantity100 tests
ApplicationsCell Separation (MojoSort™, ) - Quality tested
FormatCocktail: Phosphate-buffered solution containing 0.09% sodium azide, pH 7.2.
Streptavidin Nanobeads: Aqueous solution containing BSA and 0.05% sodium azide
ReactivityMouse
Detection TargetCD8
Cell Type (standardized)CD8+ T Cell

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(41) The potential role of CMC1 as an immunometabolic checkpoint in T cell immunityOncoImmunologyDecember 31, 2024Yuwen Chen, Jie Gao, Mingyue Ma, Ke Wang, Fangming Liu, Feiyu Yang, Xin Zou, Zhouli Cheng, Duojiao Wufrom the spleen of C57BL/6 mice using the MojoSort™ Mouse CD8+T Cell Isolation Kit (BioLegend, Cat# 480008). CD8+T cells were activated with plate-bound anti-mouse CD3e (5 μg/ml, BioLegend, Cat# 100340) and

(42) Napabucasin-loaded PLGA nanoparticles trigger anti-HCC immune responses by metabolic reprogramming of tumor-associated macrophagesJournal of Translational MedicineDecember 20, 2024Zhenwei Song, Hongfei Chen, Xueyao Wang, Zhiyue Zhang, Hui Li, Huajun Zhao, Yang Liu, Qiuju Han, Jian Zhangmacrophages The splenic CD8+T cells of mice were sorted using a CD8+T cell sorting kit (Biolegend, 480008), and then activated with anti-CD3 (Biolegend, 100340) and anti-CD28 (Biolegend, 102116) antibodies.

(43) CWF19L1 promotes T-cell cytotoxicity through the regulation of alternative splicingJournal of Biological ChemistryDecember 1, 2024Yuqi Zhang, Jingjing Yi, Gaigai Wei, Tingrong Ren, Haiping Zhao, Huiling Zhang, Hui Yang, Duanwu Zhangwere isolated from the spleen of OT-I TCR transgenic mice using a negative selection kit (BioLegend, 480008) according to the manufacturer′s instructions. A total of 1 × 10∧6 CD8+ T cells per well were seeded

(44) STING-activating dendritic cell-targeted nanovaccines that evoke potent antigen cross-presentation for cancer immunotherapyBioactive materialsDecember 1, 2024Nguyen NT, Le XT, Lee WT, Lim YT, Oh KT, Lee ES, Choi HG, Youn YS.MAX™ Mouse IFN-β ELISA kit (cat. no. 439407) and MojoSort™ Mouse CD8 T Cell isolation kit (cat. no. 480008), Alexa Fluor® 488 Goat anti-hamster (Armenian) IgG antibody (cat. no. 405508), CFSE cell division tracker kit

(45) Chemoproteomics reveals immunogenic and tumor-associated cell surface substrates of ectokinase CK2αCell Chemical BiologySeptember 19, 2024Corleone S. Delaveris, Sophie Kong, Jeff Glasgow, Rita P. Loudermilk, Lisa L. Kirkemo, Fangzhu Zhao, Fernando Salangsang, Paul Phojanakong, Juan Antonio Camara Serrano, Veronica Steri, et al.AlexaFluor700 conjugate (BioLegend, 100429), anti-mouse CD8a clone 53-6.7 Pacific Blue conjugate (BioLegend, 480007), rat anti-mouse CD25 clone PC61 BV605 conjugate (BioLegend, 102035). All antibodies were used at the

(46) Microglia LILRB4 upregulation reduces brain damage after acute ischemic stroke by limiting CD8(+) T cell recruitmentJournal of neuroinflammationAugust 31, 2024Ma Y, Zheng K, Zhao C, Chen J, Chen L, Zhang Y, Chen T, Yao X, Cai Y, Wu J.were washed and transferred to fresh RPMI-1640 medium. CD3 and CD8 negative magnetic beads (480023/480007, Biolegend, USA) were used to select the desired T cell population. Purity of the sorted cells was verified

(47) Targeting TNFRSF25 by agonistic antibodies and multimeric TL1A proteins co-stimulated CD8(+) T cells and inhibited tumor growthJournal for immunotherapy of cancerAugust 13, 2024Lyu X, Zhao L, Chen S, Li Y, Yang Y, Liu H, Yang F, Li W, Sui J.T cells were isolated from splenocytes using a MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, 480008). We stimulated purified cells with 2 µg/mL of plate-bound anti-CD3 Ab 145–2 C11 and the tested proteins

(48) Glycolysis inhibition induces anti-tumor central memory CD8+T cell differentiation upon combination with microwave ablation therapyNature CommunicationsMay 31, 2024Xinyu Tang, Xinrui Mao, Peiwen Ling, Muxin Yu, Hua Pan, Jiaming Wang, Mingduo Liu, Hong Pan, Wen Qiu, Nan Che, et al.buffer (Biolegend, Cat#480017) and were sorted by the mouse CD8+T cell isolation kit (Biolegend, Cat#480008) in accordance with the manufacturer’s instructions. Single cells of spleens of mice were resuspended

(49) The potential role of CMC1 as an immunometabolic checkpoint in T cell immunityOncoimmunologyApril 23, 2024Yuwen Chen, Jie Gao, Mingyue Ma, Ke Wang, Fangming Liu, Feiyu Yang, Xin Zou, Zhouli Cheng, Duojiao Wufrom the spleen of C57BL/6 mice using the MojoSort™ Mouse CD8+T Cell Isolation Kit (BioLegend, Cat# 480008). CD8+T cells were activated with plate-bound anti-mouse CD3e (5 μg/ml, BioLegend, Cat# 100340) and

(50) Tumor cell senescence-induced macrophage CD73 expression is a critical metabolic immune checkpoint in the aging tumor microenvironmentTheranosticsJanuary 1, 2024Deng Y, Chen Q, Yang X, Sun Y, Zhang B, Wei W, Deng S, Meng J, Hu Y, Wang Y, et al.the spleens of C57BL/6 mice according to the protocol of MojoSort™ Mouse CD8+ T Cell Isolation Kit (480008, Biolegend). BMDMs were initially treated with LLC CM for 24 h. Then replace the fresh medium and continue

(51) SIKs Regulate HDAC7 Stabilization and Cytokine Recall in Late-Stage T Cell Effector DifferentiationJournal of immunology (Baltimore, Md. : 1950)December 15, 2023Helms RS, Marin-Gonzalez A, Patel CH, Sun IH, Wen J, Leone RD, Duvall B, Gao RD, Ha T, Tsukamoto T, et al.negative isolation. CD8+ T cells were isolated using MojoSort Mouse CD8 T Cell Isolation Kits (BioLegend, 480008) using eight microliters of biotin-antibody cocktail and streptavidin beads per mouse. Untouched CD8+

(52) Interferon-γ couples CD8(+) T cell avidity and differentiation during infectionNature communicationsOctober 23, 2023Uhl LFK, Cai H, Oram SL, Mahale JN, MacLean AJ, Mazet JM, Piccirilli T, He AJ, Lau D, Elliott T, et al.12-week-old WT or IFN-γRKO OT-I mice, using the MojoSortTM CD8+ T-cell isolation kit and magnets (Biolegend, #480008 and #480019). Isolated T-cells were resuspended in complete RPMI (RPMI 1640 [Gibco, #21870-076] supplemented

(53) Vaccine-induced antigen archiving enhances local memory CD8+ T cell responses following an unrelated viral infectionResearch SquareSeptember 26, 2023Beth Tamburini, Thu Doan, Tadg Forward, Erin Lucas, Ira Fleming, Aspen Uecker-Martin, Jay Hesselberth, Thomas Morrisonand P14 CD8 + T cells were isolated using the Mojosort CD8 T cell isolation kit (Biolegend Cat. No. 480008). After CD8 negative selection, the cells were labeled with VPD or CFSE to assess proliferation. For

(54) Collagen VI deposition mediates stromal T cell trapping through inhibition of T cell motility in the prostate tumor microenvironmentMatrix BiologyAugust 1, 2023Hawley C. Pruitt, Ya Guan, Hudson Liu, Alexis E Carey, W. Nathaniel Brennen, Jiayun Lu, Corrine Joshu, Ashani Weeraratna, Tamara L. Lotan, T.S. Karin Karin Eisinger-Mathason, et al.were isolated from PBMCs by negative selection using MojoSort human T cell isolation kits (cat. No. 480008 and 480010 respectively) from Biolegend (Auburn, CA, USA) according to the manufacturer’s protocol.

(55) Tumor Cell–Intrinsic c-Myb Upregulation Stimulates Antitumor Immunity in a Murine Colorectal Cancer ModelCancer Immunol ResJuly 21, 2023Merel van Gogh, Jesus F. Glaus Garzon, Dilara Sahin, Lucia Knopfova, Petr Benes, Onur Boyman, Igor Jurisica, Lubor Borsigcontrol or Dox-treated tumor-bearing mice using the MojoSort Mouse CD8 T-Cell Isolation Kit (BioLegend, 480008). Cultured tumor cells and enriched CD8+ T cells were added to the culture in ratios 1:2 or 1:5 (tumor

(56) Single-cell RNA sequencing reveals the suppressive effect of PPP1R15A inhibitor Sephin1 in antitumor immunityiScienceFebruary 17, 2023Wang R, Zhang Y, Guo S, Pei S, Guo W, Wu Z, Wang H, Wang M, Li Y, Zhu Y, et al.

(57) The immune-metabolic crosstalk between CD3(+)C1q(+)TAM and CD8(+)T cells associated with relapse-free survival in HCCFrontiers in immunologyJanuary 1, 2023Yang Y, Sun L, Chen Z, Liu W, Xu Q, Liu F, Ma M, Chen Y, Lu Y, Fang H, et al.single-cell suspension. CD8+ T cells were purified through negatively magnetic sorting (Biolegend, Cat. 480008), activated with anti-CD3 (eBioscience, Cat.16-0031-85) and anti-CD28 (eBioscience, Cat.16-0281-85)

(58) The loss of epithelial Smad4 drives immune evasion via CXCL1 while displaying vulnerability to combinatorial immunotherapy in gastric cancerCell reportsDecember 27, 2022An HW, Seok SH, Kwon JW, Choudhury AD, Oh JS, Voon DC, Kim DY, Park JW.

(59) CD59-Regulated Ras Compartmentalization Orchestrates Antitumor T-cell ImmunityCancer immunology researchDecember 2, 2022Li L, Ding P, Lv X, Xie S, Li L, Chen J, Zhou D, Wang X, Wang Q, Zhang W, et al.mouse CD4+/CD8+ T-cell isolation kit (CD4+ T-cell isolation kit, 480033; CD8+ T-cell isolation kit, 480008; BioLegend, San Diego, CA), and the purity was confirmed by FACS. The purities of CD4+ and CD8+ T cells

(60) A tick C1q protein alters infectivity of the Lyme disease agent by modulating interferon γCell reportsNovember 22, 2022Tang X, Arora G, Matias J, Hart T, Cui Y, Fikrig E.isolated from splenocytes with MojoSort™ mouse CD4 and CD8 T cell isolation kits (BioLegend, #480005 and #480007). The purity of the isolated cells was evaluated by flow cytometry with the antibodies of FITC anti-mouse CD3

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(1)

1/10/2020Good Isolation Kit to Recover Untouched Mouse CD8+ T CellsDr. Min Hee OhWe are very happy in the end using this kit since it's very cheap per isolation. Yield and purity is very acceptable especially with transgenic mouse T cells.
(1)

1/6/2020Good BioLegend Mouse CD8 T Cells Isolation Kit Wei XuYou can use this kit to get acceptable yield and purity to isolate CD8 cells from mouse with reasonable price.

MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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MojoSort™ Mouse CD8 T Cell Isolation Kit from BioLegend

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