Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Quantity100 Test
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(81) IL-35 subunit EBI3 alleviates bleomycin-induced pulmonary fibrosis via suppressing DNA enrichment of STAT3Respiratory researchOctober 28, 2021Chen D, Zheng G, Yang Q, Luo L, Shen J.hydroxyproline in lung tissues of different treated groups was detected using the Hydroxyproline Assay Kit (ab222941, Abcam, Cambridge, UK). Cell culture and treatment The mouse primary lung epithelial cells were obtained

(82) Ceftriaxone and Melittin Synergistically Promote Wound Healing in Diabetic RatsPharmaceuticsOctober 6, 2021Nabil A. Alhakamy et al.(Houston, TX, USA), while hydroxyproline content was determined through a colorimetric kit (catalog# ab222941) supplied by Abcam (Cambridge, UK) following the manufacturer’s instructions. 2.10. qRT-PCR in Excised

(83) Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying FormulationInternational Journal of NanomedicineJune 9, 2021Abdulrahman E Koshak et al.of IL-6, TNF-α and hydroxyproline were determined using ELISA Kits (catalog # ab100772, ab236712 and ab222941, Abcam®, Cambridge, UK) as stated by the manufacturer’s instructions. RT-qPCR in Excised (Healing/Healed)

(84) Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying FormulationInt J NanomedicineJune 9, 2021Abdulrahman E Koshak, Mardi M Algandaby, Mohammad I Mujallid, Ashraf B Abdel-Naim, Nabil A Alhakamy, Usama A Fahmy, Anas Alfarsi, Shaimaa M Badr-Eldin, Thikryat Neamatallah, Mohammed Z Nasrullah, et al.of IL-6, TNF-α and hydroxyproline were determined using ELISA Kits (catalog # ab100772, ab236712 and ab222941, Abcam®, Cambridge, UK) as stated by the manufacturer’s instructions. RT-qPCR in Excised (Healing/Healed)

(85) Towards a bioengineered uterus: bioactive sheep uterus scaffolds are effectively recellularized by enzymatic preconditioningNPJ Regenerative medicineMay 21, 2021Padma AM, Carrière L, Krokström Karlsson F, Sehic E, Bandstein S, Tiemann TT, Oltean M, Song MJ, Brännström M, Hellström M.incubated for 1 h at 120 °C. The hydroxyproline content was then measured using a hydroxyproline assay kit (ab222941, Abcam, Cambridge, MA) following the manufactures instructions. Histology and immunohistochemistry

(86) Mitochondrial Transplantation Modulates Inflammation and Apoptosis, Alleviating Tendinopathy Both In Vivo and In VitroAntioxidants (Basel, Switzerland)April 28, 2021Lee JM, Hwang JW, Kim MJ, Jung SY, Kim KS, Ahn EH, Min K, Choi YS.performed following the manufacturer’s instructions for a hydroxyproline colorimetric assay (Abcam; ab222941). After breaking the tissue, proteins were treated with 10 N NaOH and HCl and then centrifuged at 10,000×

(87) Aging Suppresses Sphingosine-1-Phosphate Chaperone ApoM in Circulation Resulting in Maladaptive Organ RepairDevelopmental cellJune 22, 2020Ding BS, Yang D, Swendeman SL, Christoffersen C, Nielsen LB, Friedman SL, Powell CA, Hla T, Cao Z.Assays ,, Mouse ApoM Elisa Kit,Mlbio Biotechnology,Cat# ML722506 Hydroxyproline (Hyp) Kit,Abcam,Cat# ab222941 RNeasy Mini Kit,QIAGEN,Cat# 74104 EDU Proliferation Kit,BD Pharmingen,Cat# 565456 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, shRNA

(88) Hepatic stellate cell-specific knockout of transcriptional intermediary factor 1γ aggravates liver fibrosisThe Journal of experimental medicineJune 1, 2020Lee EJ, Hwang I, Lee JY, Park JN, Kim KC, Kim I, Moon D, Park H, Lee SY, Kim HS, et al.milliliter. Hydroxyproline assay Hydroxyproline from collagen was detected using a hydroxyproline assay kit (ab222941; Abcam) according to the manufacturer’s instructions. Briefly, 10 mg liver tissue was homogenized with

(89) 3D bioprinting via an in situ crosslinking technique towards engineering cartilage tissueScientific ReportsDecember 27, 2019Jonathan H. Galarraga, Mi Y. Kwon, Jason A. BurdickDimethylmethylene Blue (DMMB), PicoGreen, and hydroxyproline assays (Abcam Hydroxyproline Assay Kit, ab222941) were subsequently performed to quantify sulfated glycosaminoglycan (GAG), DNA, and collagen (COL) contents,

(90) Imaging Fibrogenesis in a Diet-Induced Model of Nonalcoholic Steatohepatitis (NASH)Contrast Media & Molecular ImagingDecember 1, 2019S. V. Hartimath et al.and HFD treatment. The liver was weighed, and a portion was snap frozen for hydroxyproline analysis (ab222941, Abcam) or mRNA quantification. The remaining liver tissues were fixed in 10% neutral buffered formalin

(91) KLF4 in Macrophages Attenuates TNFα-Mediated Kidney Injury and FibrosisJournal of the American Society of NephrologyOctober 1, 2019Yi Wen et al.concentrations were measured according to the manufacturer’s instructions of the Hydroxyproline Assay Kit (ab222941; Abcam). Western Blots Kidney tissues were homogenized in RIPA Buffer (R0278; Sigma-Aldrich). The concentration

(92) Twist1 in Infiltrating Macrophages Attenuates Kidney Fibrosis via Matrix Metallopeptidase 13–Mediated Matrix DegradationJournal of the American Society of NephrologySeptember 1, 2019Jiafa Ren, Jiandong Zhang, Nathan P. Rudemiller, Robert Griffiths, Yi Wen, Xiaohan Lu, Jamie R. Privratsky, Michael D. Gunn, Steven D. Crowleywere homogenized in distilled water, hydroxyproline was detected by Hydroxyproline Assay Kit (catalog ab222941; Abcam) according to the manufacturer’s instructions. The hydroxyproline content was expressed as micrograms

(93) Dendropanax morbifera Ameliorates Thioacetamide-Induced Hepatic Fibrosis via TGF-β1/Smads PathwaysInternational journal of biological sciencesJanuary 1, 2019Yang HY, Kim KS, Lee YH, Park JH, Kim JH, Lee SY, Kim YM, Kim IS, Kacew S, Lee BM, et al.dryness under vacuum at 70°C for 45 min. Hydroxyproline was measured using a hydroxyproline assay kit (ab222941, Abcam, Cambridge, MA) in accordance with manufacturer's instructions and results expressed as micromoles

(94) Exploring multiorgan mitochondrial dysfunction in the switch toward progressive MASLD in AMLN miceiScienceJanuary 1, 0001"Meroni M, Paolini E, Longo M, Battistin M, Dondossola D, Ripolone M, Napoli L, Mosca E, Corti S, Dongiovanni P."Aldrich,Cat#C5138 Clarity Western ECL Substrate,Bio-Rad,Cat#1705061 Hydroxyproline Assay Kit (Colorimetric),Abcam,Cat#AB222941 Triglyceride Assay Kit - Quantification,Abcam,Cat#AB65336 Total Cholesterol & Cholesteryl Ester Colorimetric Assay Kit

(95) Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic SteatohepatitisCell metabolismJanuary 1, 0001"Sun X, Seidman JS, Zhao P, Troutman TD, Spann NJ, Que X, Zhou F, Liao Z, Pasillas M, Yang X, et al."assay kit,Abcam,ab156065 TBARS assay kit,Cayman Chemicals,10009055 Hydroxyproline assay kit,Abcam,ab222941 Superoxide Dismutase Activity Assay Kit,Abcam,ab65354 Dil-OxLDL,Life Technologies,L34358 Dil-nLDL,Life Technologies

(96) The anti-inflammation effect of strontium ranelate on rat chondrocytes with or without IL-1β in vitroExperimental and therapeutic medicineJanuary 1, 0001"Yu H, Liu Y, Yang X, He J, Zhong Q, Guo X."collagen and does not exist in other human tissues (22). A hydroxyproline test kit (Abcam; cat. no. ab222941) was used in accordance with the manufacturer's instructions. Cell culture supernatant, ddH2O and standard

(97) L-carnosine Attenuates Bleomycin-Induced Oxidative Stress via NFκB Pathway in the Pathogenesis of Pulmonary Fibrosis"Antioxidants (Basel, Switzerland)"January 1, 0001"Park J, Jang J, Cha SR, Baek H, Lee J, Hong SH, Lee HA, Lee TJ, Yang SR."measurement of hydroxyproline in lung tissue, it was performed using hydroxyproline colorimetric assay kit (ab222941, Abcam). For sample preparation, the lung tissues were reacted with 10 N NaOH for 1 h. Additionally,

(98) Strontium ranelate promotes chondrogenesis through inhibition of the Wnt/β-catenin pathwayStem cell research & therapyJanuary 1, 0001"Yu H, Liu Y, Yang X, He J, Zhang F, Zhong Q, Guo X."staining. Cells were photographed by microscopy. Hydroxyproline (Hyp) assay Hyp assay kit (Abcam, ab222941, USA) was used according to the manufacturers instructions. The cell culture supernatants, ddH2O, and

(99) Somatic gene editing ameliorates skeletal and cardiac muscle failure in pig and human models of Duchenne muscular dystrophyNature medicineJanuary 1, 0001"Moretti A, Fonteyne L, Giesert F, Hoppmann P, Meier AB, Bozoglu T, Baehr A, Schneider CM, Sinnecker D, Klett K, et al."assay Hydroxyproline in porcine tissue samples was quantified via Colorimetric Hydroxyproline Assay Kit (ab222941, Abcam, Cambridge, UK). 100 mg of each sample was homogenized in 300 μl and subjected to alkaline lysis

(100) Let-7 restrains an epigenetic circuit in AT2 cells to prevent fibrogenic intermediates in pulmonary fibrosisNature communicationsJanuary 1, 0001"Seasock MJ, Shafiquzzaman M, Ruiz-Echartea ME, Kanchi RS, Tran BT, Simon LM, Meyer MD, Erice PA, Lotlikar SL, Wenlock SC, et al."(Sigma, CS0030) following manufacturer instructions. Hydroxyproline Colorimetric Assay Kit (Abcam, ab222941) was used to measure collagen in lung lobes following kit instructions and optical density was measured

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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