Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Quantity100 Test
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(21) Regular aerobic exercise ameliorates radiation-induced pulmonary fibrosis: An animal studyClinical and Translational Radiation OncologyJuly 1, 2025Da Bin Ko, Seungwoo Cha, Jae Yong Jeon, Su SSan Kim, Won Kim, Sei Won Lee, Joongkee Min, Si Yeol Song, Seung Hak Leeanother direct indicator of pulmonary fibrosis, was estimated using the hydroxyproline (HYP) assay kit (ab222941, Abcam, USA). In brief, the tissue was homogenized (10 mg per 100 µL D.W), and 100 µL of the sample

(22) MicroRNAs regulate alveolar macrophage homeostasis and its function in lung fibrosisFrontiers in ImmunologyJune 23, 2025Nirmal Parajuli, Yi Yao, Namir Khalasawi, Congcong Yin, Qiong Zhang, Indra Adrianto, Aakash Hans, Li Zhou, Qing-Sheng Mihydroxyproline content was quantified using the colorimetric Hydroxyproline Assay Kit (Abcam, catalog # Ab222941), while H&E- and trichome-stained slides were imaged using the Olympus FSX100 Inverted Microscope (Olympus)

(23) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJournal of the American Heart AssociationJune 17, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaT5168 (Sigma Aldrich) as a control. Biochemical Assays Hydroxyproline was evaluated using Abcam kit (ab222941) according to manufacturer's instructions (Abcam, Cambridge, UK). ATP levels were assessed using a fluorometric

(24) Omaveloxolone, But Not Dimethyl Fumarate, Improves Cardiac Function in Friedreich's Ataxia Mice With Severe CardiomyopathyJ Am Heart AssocJune 12, 2025Lili Salinas, Francisco Figueroa, Claire B. Montgomery, Phung N. Thai, Nipavan Chiamvimonvat, Gino Cortopassi, Elena N. DedkovaT5168 (Sigma Aldrich) as a control. Biochemical Assays Hydroxyproline was evaluated using Abcam kit (ab222941) according to manufacturer's instructions (Abcam, Cambridge, UK). ATP levels were assessed using a fluorometric

(25) Ferret model of bleomycin-induced lung injury shares features of human idiopathic pulmonary fibrosisBIORXIVMay 14, 2025Shuang Wu, Ian Drive, Meihui Luo, Hikaru Miyazaki, Smitha Shambhu, Dimitry Popov, Liyuan Yang, Jing Wang, Jia Ma, Junfeng Guo, et al.isolation of total protein. The HYP content of each sample was measured using a hydroxyproline assay kit (Ab222941, Abcam, Waltham, MA) according to the manufacturer’s instructions. The HYP content of plasma is presented

(26) A novel phosphodiesterase inhibitor for the treatment of chronic liver injury and metabolic diseasesHepatologyApril 1, 2025Dalton W. Staller, Sanjali S. Panigrahi, Yahani P. Jayasinghe, Yuxiang Dong, Sohan Mahto, Virender Kumar, Donald R. Ronning, Ram I. MahatoCyclic AMP ELISA Kit (Cayman Chemicals Item No. 581001) and Hydroxyproline Colorimetric Assay Kit (Abcam ab222941) according to the manufacturer’s instructions. 2.15 Fluorescence Polarization Assay The inhibitory

(27) Activated cardiac fibroblasts are a primary source of high-molecular-weight hyaluronan productionAmerican Journal of Physiology-Cell PhysiologyMarch 1, 2025Danielle T. Little, Caitlin M. Howard, Emma Pendergraft, Kenneth R. Brittian, Timothy N. Audam, Exile W. Lukudu, Juliette Smith, Daniel Nguyen, Yoshihiro Nishida, Yu Yamaguchi, et al.hydroxyproline concentration in ECMs were quantified using a commercial hydroxyproline assay (Abcam Ab222941). Confirmation of decellularization is shown in Supplemental Figure 2. Results Hyaluronan accumulates

(28) RTN3 regulates collagen biosynthesis and profibrotic macrophage differentiation to promote pulmonary fibrosis via interacting with CRTH2Mol MedFebruary 19, 2025Chen-Yu Wang, Ya-Qin Chen, Hao Huang, Zhuang-Zhuang Yuan, Yi Dong, Jie-Yuan Jin, Jie-Yi Long, Lv Liu, Liang-Liang Fan, Rong XiangEnzyme-linked immunosorbent assay (ELISA) Kit were purchased from Shanghai Jianglai Industry Co., Ltd. Hydroxyproline Assay Kit (ab222941) were purchased from Abcam company. Protein A + G beads (P2108) was purchased from Beyotime Biotechnology

(29) Enhanced Bioprinting of 3D Corneal Stroma Patches with Reliability, Assessing Product Consistency and Quality through Optimized Electron Beam SterilizationAdvanced Healthcare MaterialsFebruary 10, 2025Jungbin Yoon, Yeon‐ju Lee, Minji Kim, Ju Young Park, Jinah Janga convenient proxy for estimating collagen content. A Hydroxyproline Assay kit (Abcam, Catalog No. ab222941, USA) was used to measure collagen following the manufacturer's recommended protocol. A papain‐digested

(30) Fluorofenidone alleviates cigarette smoke exposure-induced chronic lung injury by targeting ferroptosisScientific reportsDecember 30, 2024Wu Y, Li B, Xuan Y, Jiang Y, Chen J, Liao H, Feng J, Zhang J.purchased from Fine Test Biotechnology (Wuhan, Hubei, China). Hydroxyproline assay kit (colorimetric) (ab222941) was purchased from Abcam (Cambridge, UK). Lipoprotein oxidation (MDA) detection kit (S0131S), glutathione

(31) Matricellular protein CCN1 promotes collagen alignment and scar integrity after myocardial infarctionMatrix BiologyNovember 1, 2024Annalara G. Fischer, Erin M. Elliott, Kenneth R. Brittian, Lauren Garrett, Ghazal Sadri, Julia Aebersold, Richa A. Singhal, Yibing Nong, Andrew Leask, Steven P. Jones, et al.Assay. Myocardial collagen concentration was assessed using a colorimetric hydroxyproline assay kit (Abcam, Cat. # Ab222941). Initially, approximately 100 mg of cardiac tissue was homogenized in 74 μL of dH2O per 10 mg tissue,

(32) Cardiac and skeletal muscle manifestations in the G608G mouse model of Hutchinson‐Gilford progeria syndromeAging CellOctober 1, 2024Yeojin Hong, Alice Rannou, Nancy Manriquez, Jack Antich, Weixin Liu, Mario Fournier, Ariel Omidfar, Russell G. Rogersand stored at −80°C. Hydroxyproline was measured on frozen tissues using a Hydroxyproline Assay Kit (#ab222941, Abcam), according to the manufacturer's protocol. 2.7 Immunohistochemistry Heart and TA cryosections

(33) Exploring the Wound Healing Potential of HispidinNutrientsSeptember 19, 2024Liu YS, Lai MC, Hong TY, Liu IM.hexosamine, and hexuronic acid using commercially available ELISA assay kits. The hydroxyproline ELISA kit (#ab222941) was obtained from Abcam Inc. (Cambridge, MA, USA) and operates within the absorbance band centered

(34) Carthamus tinctorius L. inhibits hepatic fibrosis and hepatic stellate cell activation by targeting the PI3K/Akt/mTOR pathwayMolecular Medicine ReportsAugust 26, 2024Zhiheng Dong, Haibin Guan, Lu Wang, Lijuan Liang, Yifan Zang, Lan Wu, Lidao Baosupernatant was collected for measurement of hydroxyproline with a Hydroxyproline Assay kit (cat. no. ab222941; Abcam), according to the manufacturer's instructions. Total protein was used as an internal reference. Statistical

(35) Targeting CEBPA to restore cellular identity and tissue homeostasis in pulmonary fibrosisJCI insightJuly 16, 2024Tan Q, Wellmerling JH, Song S, Dresler SR, Meridew JA, Choi KM, Li Y, Prakash YS, Tschumperlin DJ.measuring collagen. Collagen content in the lung was measured using a hydroxyproline assay kit (Abcam, ab222941) according to the manufacturer’s instructions, as previously described (61). Frozen lung tissue was

(36) Boiling histotripsy exhibits anti-fibrotic effects in animal models of liver fibrosisScientific ReportsJuly 2, 2024Chanmin Joung, Jeongmin Heo, Ki Joo Pahk, Kisoo Pahkassay The total collagen content in the liver was quantified using a colorimetric hydroxyproline assay kit (ab222941, Abcam, Cambridge, MA, USA). Liver tissues were denatured with a papain solution, as previously described33,

(37) A Novel Phosphodiesterase Inhibitor for the Treatment of Chronic Liver Injury and Metabolic DiseasesHepatologyJuly 1, 2024Dalton W. Staller, Sanjali S. Panigrahi, Yahani P. Jayasinghe, Yuxiang Dong, Sohan Mahto, Virender Kumar, Donald R. Ronning, Ram I. MahatoCyclic AMP ELISA Kit (Cayman Chemicals Item No. 581001) and Hydroxyproline Colorimetric Assay Kit (Abcam ab222941) according to the manufacturer’s instructions. 2.15 Fluorescence Polarization Assay The inhibitory

(38) Injectable MSC Spheroid and Microgel Granular Composites for Engineering TissueAdvanced MaterialsApril 1, 2024Nikolas Di Di Caprio, Matthew D. Davidson, Andrew C. Daly, Jason A. BurdickCollagen Biochemical Analysis. Total collagen content was measured with a hydroxyproline assay kit (Abcam, ab222941) according to manufacturer’s protocol. Briefly, 10 N NaOH was added to each sample, heated to 120 °C

(39) Modified Qing-Zao-Jiu-Fei decoction attenuated pulmonary fibrosis induced by bleomycin in rats via modulating Nrf2/NF-κB and MAPKs pathwaysChinese medicineJanuary 16, 2024Zhu JQ, Tian YY, Chan KL, Hu Z, Xu QQ, Lin ZX, Xian YF.level in the lung tissues of rats were measured using the hydroxyproline assay kit obtained from Abcam (Ab222941). Briefly, lung issues (100 mg) were homogenized in 100 μL of dH2O. Then, 100 μL of sample homogenate

(40) Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillationTheranosticsJanuary 1, 2024Parent S, Vaka R, St Amant J, Kahn S, Van Remortel S, Bi C, Courtman D, Stewart DJ, Davis DR.quantified using ImageJ 37, 38. Left atria collagen content was confirmed using an assay for hydroxyproline (ab222941, Abcam), an indicator of collagen content/turnover 39, 40. Homogenized atrial tissue was analyzed for

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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