Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Quantity100 Test
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(41) Injectable MSC Spheroid and Microgel Granular Composites for Engineering TissueAdvanced MaterialsApril 1, 2024Nikolas Di Di Caprio, Matthew D. Davidson, Andrew C. Daly, Jason A. BurdickCollagen Biochemical Analysis. Total collagen content was measured with a hydroxyproline assay kit (Abcam, ab222941) according to manufacturer’s protocol. Briefly, 10 N NaOH was added to each sample, heated to 120 °C

(42) Modified Qing-Zao-Jiu-Fei decoction attenuated pulmonary fibrosis induced by bleomycin in rats via modulating Nrf2/NF-κB and MAPKs pathwaysChinese medicineJanuary 16, 2024Zhu JQ, Tian YY, Chan KL, Hu Z, Xu QQ, Lin ZX, Xian YF.level in the lung tissues of rats were measured using the hydroxyproline assay kit obtained from Abcam (Ab222941). Briefly, lung issues (100 mg) were homogenized in 100 μL of dH2O. Then, 100 μL of sample homogenate

(43) Inactivation of the NLRP3 inflammasome mediates exosome-based prevention of atrial fibrillationTheranosticsJanuary 1, 2024Parent S, Vaka R, St Amant J, Kahn S, Van Remortel S, Bi C, Courtman D, Stewart DJ, Davis DR.quantified using ImageJ 37, 38. Left atria collagen content was confirmed using an assay for hydroxyproline (ab222941, Abcam), an indicator of collagen content/turnover 39, 40. Homogenized atrial tissue was analyzed for

(44) Lysophosphatidic acid receptor 1 antagonist (EPGN2154) causes regression of NASH in preclinical NASH modelsHepatology CommunicationsDecember 1, 2023Jashdeep Bhattacharjee, Graham Beaton, Satheesh B. Ravula, Suk Joong Lee, Kevin B. Bacon, Celia P. Jenkinson, Mikako Warren, Fabio C. Tucci, Rohit Kohlimg) was used to quantify the hydroxyproline content using Hydroxyproline Assay Kit (Colorimetric) (ab222941) from Abcam (Boston, MA) according to the manufacturer’s instructions. LPAR1 expression Total RNA

(45) Superiority of systemic bleomycin to intradermal HOCl for the study of interstitial lung diseaseScientific ReportsNovember 23, 2023Arina Morozan, Sydney Joy, Utako Fujii, Richard Fraser, Kevin Watters, James G. Martin, Inés Colmegnaassay Lung hydroxyproline content was analyzed with a hydroxyproline colorimetric assay kit (Abcam, Ab222941) following manufacturer’s instruction. The lungs from PBS and HOCl mice were hydrolyzed in 10N NaOH

(46) Lysophosphatidic acid receptor 1 antagonist (EPGN2154) causes regression of NASH in preclinical NASH modelsHepatol CommunNovember 22, 2023Jashdeep Bhattacharjee, Graham Beaton, Satheesh B. Ravula, Suk Joong Lee, Kevin B. Bacon, Celia P. Jenkinson, Mikako Warren, Fabio C. Tucci, Rohit Kohlimg) was used to quantify the hydroxyproline content using Hydroxyproline Assay Kit (Colorimetric) (ab222941) from Abcam (Boston, MA) according to the manufacturer’s instructions. LPAR1 expression Total RNA

(47) Effect of the Functional VP1 Unique Region of Human Parvovirus B19 in Causing Skin Fibrosis of Systemic SclerosisInternational Journal of Molecular SciencesOctober 18, 2023Der-Yuan Chen, Chih-Chen Tzang, Chuan-Ming Liu, Tsu-Man Chiu, Jhen-Wei Lin, Pei-Hua Chuang, Chia-Wei Kuo, Bor-Show Tzang, Tsai-Ching HsuAssay The contents of skin collagen were detected using a Hydroxyproline Assay Kit (Colori metric) (Ab222941, Abcam, Cambridge, UK). Briefly, tissue samples were weighted and pooled with 100 μL ddH2O per 10 mg

(48) Extremophilic Solutions: The Role of Deinoxanthin in Counteracting UV-Induced Skin HarmCurrent Issues in Molecular BiologyOctober 16, 2023Mehmet Kuzucuthe cell lysates was measured using Abcam’s Hydroxyproline Assay Kit (Colorimetric) (Product code: ab222941) according to the manufacturer’s instructions. The absorbance was measured at 560 nm using a microplate

(49) Altered expression, but small contribution, of the histone demethylase KDM6A in obstructive uropathy in miceDisease models & mechanismsSeptember 1, 2023Hong LYQ, Yeung ESH, Tran DT, Yerra VG, Kaur H, Kabir MDG, Advani SL, Liu Y, Batchu SN, Advani A.content Kidney hydroxyproline content was determined using a Hydroxyproline Assay Kit (Colorimetric) (ab222941, Abcam) and normalized to total protein measured by Quick Start™ Bradford 1× Dye Reagent (5000205, Bio-Rad,

(50) Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutritionJ Clin InvestSeptember 1, 2023Cheen Fei Chin, Dwight L.A. Galam, Liang Gao, Bryan C. Tan, Bernice H. Wong, Geok-Lin Chua, Randy Y.J. Loke, Yen Ching Lim, Markus R. Wenk, Miao-Shan Lim, et al.quantification. Liver hydroxyproline was quantified using the Colorimetric Hydroxyproline Assay Kit (ab222941, Abcam). In brief, frozen liver was homogenized in nuclease-free water (10 ml water/mg tissue) using

(51) Blood-derived lysophospholipid sustains hepatic phospholipids and fat storage necessary for hepatoprotection in overnutritionJournal of Clinical InvestigationSeptember 1, 2023Cheen Fei Chin, Dwight L.A. Galam, Liang Gao, Bryan C. Tan, Bernice H. Wong, Geok-Lin Chua, Randy Y.J. Loke, Yen Ching Lim, Markus R. Wenk, Miao-Shan Lim, et al.quantification. Liver hydroxyproline was quantified using the Colorimetric Hydroxyproline Assay Kit (ab222941, Abcam). In brief, frozen liver was homogenized in nuclease-free water (10 ml water/mg tissue) using

(52) Euterpe oleracea extract (açaí) exhibits cardioprotective effects after chemotherapy treatment in a breast cancer modelBMC Complementary Medicine and TherapiesAugust 25, 2023Jéssica Alessandra-Perini, Daniel Escorsim Machado, Celia Yelimar Palmero, Marllow Caetano Claudino, Roberto Soares de Moura, Antônio Palumbo Junior, Jamila Alessandra Perini, Luiz Eurico Nasciuttiused. The concentration of hydroxyproline in the rat cardiac tissues were measured with an ELISA kit (ab222941 Abcam, Cambridge, UK) following the manufacturer’s protocol. Statistical analysis Continuous data

(53) The effects of a comparatively higher dose of 1000 mg/kg/d of oral L- or D-arginine on the L-arginine metabolic pathways in male Sprague-Dawley ratsPLoS OneAugust 1, 2023Dain (Raina) Kim, Sarah Martin, Kaushik Desaispectrophotometer. Hydroxyproline levels were measured with a colorimetric Hydroxyproline Assay Kit (Cat. # ab222941, Abcam Inc., Toronto, ON, Canada), according to provided instructions. The absorbance was measured with

(54) Hypoxanthine Induces Signs of Bladder Aging with Voiding dysfunction and Lower Urinary Tract RemodelingThe journals of gerontology. Series A, Biological sciences and medical sciencesJuly 18, 2023Birder LA, Wolf-Johnston AS, Zabbarova I, Ikeda Y, Robertson AM, Cardozo R, Azari F, Kanai AJ, Kuchel GA, Jackson EK.structural changes. In brief, hydroxyproline was detected in hydrolysates by a colorimetric assay (Abcam AB222941, Waltham, MA). Statistics Data were analyzed in GraphPad Prism 6 using Student’s t tests (2-tailed)

(55) Tumor Growth Ameliorates Cardiac DysfunctionCellsJuly 14, 2023Lama Awwad, Rona Shofti, Tali Haas, Ami Aronheimfibrosis content in cardiac cell lysates using a colorimetric hydroxyproline assay kit (Abcam, USA, Ab222941). The assay was performed according to the manufacturer’s instructions. 2.11. Heart Single-Cell Suspension

(56) Collagen and Beyond: A Comprehensive Comparison of Human ECM Properties Derived from Various Tissue Sources for Regenerative Medicine ApplicationsJournal of Functional BiomaterialsJuly 11, 2023Nashaita Y. Patrawalla, Nilabh S. Kajave, Mohammad Z. Albanna, Vipuil Kishorepurchased from Bio-Rad (Hercules, CA, USA). Hydroxyproline assay kit was purchased from Abcam (Cat# ab222941/K226) (Cambridge, UK). CMSCs were isolated in-house from the cord tissues. All other chemicals and reagents

(57) Suspension bath bioprinting and maturation of anisotropic meniscal constructsBiofabricationJuly 1, 2023Margaret E. Prendergast, Su-Jin Heo, Robert L. Mauck, Jason A. Burdick(Picogreen assay kit, Invitrogen P7589) and hydroxyproline assays (Abcam hydroxyproline assay kit, Abcam, ab222941) were then performed to quantify sulfated glycosaminoglycan (GAG), DNA, and collagen (COL) contents,

(58) Evaluation of surgical fixation methods for the implantation of melt electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defectsInternational Journal of BioprintingJune 14, 2023Jonathan H. H. Galarraga, Hannah M. M. Zlotnick, Ryan C. C. Locke, Sachin Gupta, Natalie L. L. Fogarty, Kendall M. M. Masada, Brendan D. D. Stoeckl, Lorielle Laforest, Miguel Castilho, Jos Malda, et al.via the dimethylmethylene blue assay, the hydroxyproline (OHP) assay (Abcam Hydroxyproline Assay Kit, ab222941), and the Picogreen dsDNA assay, respectively[24]. Separate samples were processed for 496histology

(59) Reinforcement of Hydrogels with a 3D-Printed Polycaprolactone (PCL) Structure Enhances Cell Numbers and Cartilage ECM Production under CompressionJournal of Functional BiomaterialsJune 7, 2023Hamed Alizadeh Sardroud, Xiongbiao Chen, B. Frank Eamesprotocol that was previously established [28]. HP was measured using a hydroxyproline assay kit (Abcam, ab222941). The remainder of the digested samples used for the DMMB assay were used for the HP assay. Digested

(60) UDP-glucuronate metabolism controls RIPK1-driven liver damage in nonalcoholic steatohepatitisNature communicationsMay 11, 2023Zhang T, Zhang N, Xing J, Zhang S, Chen Y, Xu D, Gu J.with 1 N HCl. Hydroxyproline in supernatant was measured with Hydroxyproline assay kit (Abcam, Cat. Ab222941), according to the manufacturer’s instruction. Histology and immunostaining Tissues were harvested

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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