Hydroxyproline Assay Kit (Colorimetric) ab222941 from Abcam

Product Specs
ItemHydroxyproline Assay Kit (Colorimetric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab222941
Quantity100 Test
Sample TypeUrine, Serum, Other biological fluids, Tissue Homogenate
Assay Time3h 0m
Detection Range0.1 - 1 µg

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Cambridge Biomedical Campus

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(61) Cordycepin- melittin nanoconjugate intensifies wound healing efficacy in diabetic ratsSaudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical SocietyMay 1, 2023Shaik RA, Alotaibi MF, Nasrullah MZ, Alrabia MW, Asfour HZ, Abdel-Naim AB.Similarly, the skin tissue hydroxyproline concentration was estimated employing a suitable kit (catalog # ab222941, Abcam®, Cambridge, UK) as per the manufacturer’s directions. 2.13 Immunohistochemical assessment of

(62) Expression of Adipose Tissue Extracellular Matrix-Related Genes Predicts Weight Loss after Bariatric SurgeryCellsApril 26, 2023Óscar Osorio-Conles, Romina Olbeyra, Josep Vidal, Ainitze Ibarzabal, José María Balibrea, Ana de Hollandameasure of total collagen, were performed in SAT lysates using a colorimetric hydroxyproline assay kit (Ab222941, Abcam, Cambridge, UK). Absorbance was measured at 560 nm in an Infinite M Plex plate reader (Tecan,

(63) Effects of the Oral Angiotensin II Type 2 Receptor Agonist C21 in Sugen-Hypoxia Induced Pulmonary Hypertension in RatsInternational Journal of Molecular SciencesApril 19, 2023Göran Tornling, Rohit Batta, Dan Salvail, Johan Raud, Christopher P. DentonHydroxyproline Content Hydroxyproline in the right lung was quantified using a commercial kit from Abcam (Ab222941) used as per the manufacturer’s instructions. The right lobes were homogenized in PBS and hydrolyzed

(64) Applied Compressive Strain Governs Hyaline-like Cartilage versus Fibrocartilage-like ECM Produced within Hydrogel ConstructsInternational Journal of Molecular SciencesApril 18, 2023Hamed Alizadeh Sardroud, Xiongbiao Chen, B. Frank Eameshydroxyproline assay were also prepared using the dissolution protocol above using a kit purchased from Abcam (ab222941). The samples were vortexed, and then hydrolyzed in a microtube at 100 °C for 4 h with the same amount

(65) C/EBP homologous protein promotes Sonic Hedgehog secretion from type II alveolar epithelial cells and activates Hedgehog signaling pathway of fibroblast in pulmonary fibrosisRespiratory ResearchDecember 1, 2022Xiaoyu Yang, Wei Sun, Xiaoyan Jing, Qian Zhang, Hui Huang, Zuojun Xuconcentration of hydroxyproline was measured using Hydroxyproline Assay Kit (Colorimetric) (Abcam, ab222941) according to the manufacturer’s protocol. Western blot Total proteins were collected by cell lysis

(66) METTL3 enhances NSD2 mRNA stability to reduce renal impairment and interstitial fibrosis in mice with diabetic nephropathyBMC NephrologyDecember 1, 2022Weiming Tang, Yilin Zhao, Hui Zhang, Ying Peng, Zhilian RuiCambridge, MA, USA), monocyte chemotactant protein-1 (MCP-1, ab100722, Abcam), and hydroxyproline (ab222941, Abcam) were determined adhering to the instructions of the ELISA kits. Cell treatment A mouse mesangial cell

(67) Salt-inducible kinase 2 (SIK2) inhibitor ARN-3236 attenuates bleomycin-induced pulmonary fibrosis in miceBMC Pulmonary MedicineDecember 1, 2022Liangneng Zou, Dequn Hong, Kecong Li, Bingyuan Jiangwas collected, and the hydroxyproline content was evaluated with a hydroxyproline assay kit (Abcam, Ab222941) following the manufacturer’s instructions. The results were expressed as μg hydroxyproline/ mg lung

(68) CD147 contributes to SARS-CoV-2-induced pulmonary fibrosisSignal transduction and targeted therapyNovember 25, 2022Wu J, Chen L, Qin C, Huo F, Liang X, Yang X, Zhang K, Lin P, Liu J, Feng Z, et al.of the right lung was selected for examination. According to the manufacturer’s instructions (Abcam, ab222941), 10 N concentrated NaOH was added to samples and hydrolyzed at 120 °C for 1 h. After cooling on ice,

(69) The aldolase inhibitor aldometanib mimics glucose starvation to activate lysosomal AMPKNature metabolismOctober 1, 2022Zhang CS, Li M, Wang Y, Li X, Zong Y, Long S, Zhang M, Feng JW, Wei X, Liu YH, et al.Cambridge Isotope Laboratories. Picro Sirius Red Stain kit (ab150681) and Hydroxyproline Assay kit (ab222941) were purchased from Abcam. DAPI Fluoromount-G mountant (0100-20) was purchased from SouthernBiotech.

(70) Novel Nanoconjugate of Apamin and Ceftriaxone for Management of Diabetic WoundsLife (Basel)July 21, 2022Abdullah A. Alamoudi, Awaad S. Alharbi, Ashraf B. Abdel-Naim, Shaimaa M. Badr-Eldin, Zuhier A. Awan, Solomon Z. Okbazghi, Osama A. A. Ahmed, Nabil A. Alhakamy, Usama A. Fahmy, Ahmed EsmatArbor, MI, USA). The hydroxyproline content in the skin was determined using an ELISA kit (catalog # ab222941, Abcam®, Cambridge, UK), based on the instructions provided in the product pamphlet. 2.2.12. RT-qPCR

(71) 3D Bioprinting of Multi-Material Decellularized Liver Matrix Hydrogel at Physiological TemperaturesBiosensors (Basel)July 13, 2022Vamakshi Khati, Harisha Ramachandraiah, Falguni Pati, Helene A. Svahn, Giulia Gaudenzi, Aman RussomGAGs were each characterized in 10 mg of native and decellularized tissue. A hydroxyproline assay kit (ab222941, Abcam, Cambridge, UK) was used to quantify collagen according to the manufacturer’s protocol. Briefly,

(72) Endoplasmic reticulum stress modulates the fate of lung resident mesenchymal stem cell to myofibroblast via C/EBP homologous protein during pulmonary fibrosisStem cell research & therapyJune 28, 2022Yang X, Sun W, Jing X, Zhang Q, Huang H, Xu Z.concentration of hydroxyproline was measured using Hydroxyproline Assay Kit (Colorimetric) (Abcam, ab222941) according to the manufacturer's protocol. Histology staining Hematoxylin and eosin (HE) and Masson’s

(73) Collagen type XIX regulates cardiac extracellular matrix structure and ventricular functionMatrix BiologyMay 1, 2022Ghazal Sadri, Annalara G. Fischer, Kenneth R. Brittian, Erin Elliott, Matthew A. Nystoriak, Shizuka Uchida, Marcin Wysoczynski, Andrew Leask, Steven P. Jones, Joseph B. Moorecontent Myocardial collagen content was enumerated using a colorimetric hydroxyproline assay kit (Abcam, Cat. Ab222941) according to the manufacturer’s instructions. Briefly, 100 mg of isolated heart tissue was homogenized

(74) Injectable decellularized nucleus pulposus tissue exhibits neuroinhibitory propertiesJOR SPINEMarch 1, 2022Logan M. Piening, David J. Lillyman, Fei San Lee, Alvaro Moreno Lozano, Jeremy R. Miles, Rebecca A. WachsA value of 13.5% was used as the percentage of hydroxyproline in collagen. A hydroxyproline assay (ab222941, Abcam) was conducted according to the manufacturer's instructions with slight modifications to determine

(75) Fabrication of MSC-laden composites of hyaluronic acid hydrogels reinforced with MEW scaffolds for cartilage repairBiofabricationJanuary 1, 2022Jonathan H. Galarraga, Ryan C. Locke, Claire E. Witherel, Brendan D Stoeckl, Miguel D. Castilho, Robert L. Mauck, Jos Malda, Riccardo Levato, Jason A. Burdickhydroxyproline (OHP) assay was performed to determine collagen content (Abcam Hydroxyproline Assay Kit, ab222941), and the Picogreen dsDNA assay was performed to measure total DNA content within cultured constructs

(76) Deletion of Glut1 in early postnatal cartilage reprograms chondrocytes toward enhanced glutamine oxidationBone ResearchDecember 1, 2021Cuicui Wang, Jun Ying, Xiangfeng Niu, Xiaofei Li, Gary J. Patti, Jie Shen, Regis J. O’Keeferemoved from plates and examined for hydroxyproline content using a Hydroxyproline Assay Kit (Abcam; #ab222941). The hydroxyproline content was normalized to the tissue weight. Glucose and glutamine labeling experiments

(77) IL-35 subunit EBI3 alleviates bleomycin-induced pulmonary fibrosis via suppressing DNA enrichment of STAT3Respiratory researchOctober 28, 2021Chen D, Zheng G, Yang Q, Luo L, Shen J.hydroxyproline in lung tissues of different treated groups was detected using the Hydroxyproline Assay Kit (ab222941, Abcam, Cambridge, UK). Cell culture and treatment The mouse primary lung epithelial cells were obtained

(78) Ceftriaxone and Melittin Synergistically Promote Wound Healing in Diabetic RatsPharmaceuticsOctober 6, 2021Nabil A. Alhakamy et al.(Houston, TX, USA), while hydroxyproline content was determined through a colorimetric kit (catalog# ab222941) supplied by Abcam (Cambridge, UK) following the manufacturer’s instructions. 2.10. qRT-PCR in Excised

(79) Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying FormulationInternational Journal of NanomedicineJune 9, 2021Abdulrahman E Koshak et al.of IL-6, TNF-α and hydroxyproline were determined using ELISA Kits (catalog # ab100772, ab236712 and ab222941, Abcam®, Cambridge, UK) as stated by the manufacturer’s instructions. RT-qPCR in Excised (Healing/Healed)

(80) Wound Healing Activity of Opuntia ficus-indica Fixed Oil Formulated in a Self-Nanoemulsifying FormulationInt J NanomedicineJune 9, 2021Abdulrahman E Koshak, Mardi M Algandaby, Mohammad I Mujallid, Ashraf B Abdel-Naim, Nabil A Alhakamy, Usama A Fahmy, Anas Alfarsi, Shaimaa M Badr-Eldin, Thikryat Neamatallah, Mohammed Z Nasrullah, et al.of IL-6, TNF-α and hydroxyproline were determined using ELISA Kits (catalog # ab100772, ab236712 and ab222941, Abcam®, Cambridge, UK) as stated by the manufacturer’s instructions. RT-qPCR in Excised (Healing/Healed)

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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Hydroxyproline Assay Kit (Colorimetric) from Abcam

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