DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(161) TNF-α-Mediated Endothelial Cell Apoptosis Is Rescued by Hydrogen SulfideAntioxidants (Basel)March 16, 2023Lorena Diaz Sanchez, Lissette Sanchez-Aranguren, Keqing Wang, Corinne M. Spickett, Helen R. Griffiths, Irundika H. K. DiasAfter treatments, fresh medium containing 2.5 µM SF7-AM was added and incubated at 37 °C for 30 min. DAPI (#ab228549, Abcam, Cambridge, UK), as a counterstain for nuclear morphology, was incubated at 37 °C for 10 min.

(162) Opto-electromechanical quantification of epithelial barrier function in injured and healthy airway tissuesAPL BioengineeringMarch 1, 2023Jiawen Chen, Seyed Mohammad Mir, Maria R. Hudock, Meghan R. Pinezich, Panpan Chen, Matthew Bacchetta, Gordana Vunjak-Novakovic, Jinho Kimantibody, the tissues samples were incubated with DAPI solution (4′,6-diamidino-2-phenylindole; Cat. No. ab228549, Abcam) for 10 min to stain cell nuclei. Finally, the stained tissues were placed onto a glass slide

(163) Emodin alleviates lung ischemia–reperfusion injury by suppressing gasdermin D‐mediated pyroptosis in ratsThe Clinical Respiratory JournalMarch 1, 2023Tao Jin, Fen Ai, Jin Zhou, Lin Kong, Zhangming Xiong, Dingping Wang, Ruilin Lu, Zhen Chen, Muxi Zhangwith secondary antibody (Alexa Fluor 488; Beyotime) for 1 h at room temperature in the dark. DAPI (ab228549; Abcam) was used for nuclear staining. The staining pattern was imaged using a fluorescence microscope

(164) A conditional null allele of Dync1h1 enables targeted analyses of dynein roles in neuronal length sensingJournal of cell scienceMarch 1, 2023Di Pizio A, Marvaldi L, Birling MC, Okladnikov N, Dupuis L, Fainzilber M, Rishal I.donkey anti-rabbit-IgG conjugated to Alexa Fluor 647 (1:1000; Jackson ImmunoResearch) and DAPI (Abcam, ab228549, 1:5000). After three washes in 1× PBS, coverslips were rinsed in ddH2O and mounted with Fluoromount-GTM. Immunohistochemistry

(165) Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in HypoxiaTranslational Vision Science & TechnologyFebruary 10, 2023Victoria Messerschmidt, Wen Ren, Michael Tsipursky, Joseph Irudayarajcontrol and cells treated with TACS-nuclease were treated as positive controls. DAPI staining solution (ab228549, Abcam, Cambridge, UK) was used to stain the nuclei following the manufacturer's instructions. DAPI

(166) p15INK4B is an alternative marker of senescent tumor cells in colorectal cancerHeliyonFebruary 1, 2023Soon Sang Park, Young-Kyoung Lee, So Hyun Park, Su Bin Lim, Yong Won Choi, Jun Sang Shin, Young Hwa Kim, Jang-Hee Kim, Tae Jun Parkstabilization (48 h). To check heterochromatin foci in senescence models, 1:10,000 diluted DAPI solution (ab228549, Abcam, Cambridge, UK) was stained and cells were observed under fluorescence microscopy (AXIO Imager.M1,

(167) Estradiol-17β [E2] stimulates wound healing in a 3D in vitro tissue-engineered vaginal wound modelJournal of Tissue EngineeringJanuary 27, 2023Sarah Shafaat, Sabiniano Roman Regueros, Christopher Chapple, Sheila MacNeil, Vanessa Hearndentissue sections were counterstained with DAPI (4′,6-diamidino-2-phenylindole, λex359 nm: λem457 nm) (ab228549) (1:800 dilution; Abcam) and mounted with DPX to be imaged with an epifluorescence microscope (Olympus

(168) Napyradiomycin A4 and Its Relate Compounds, a New Anti-PRV Agent and Their Antibacterial Activities, from Streptomyces kebangsaanensis WS-68302MoleculesJanuary 8, 2023Yani Zhang, Wei Fang, Kaimei Wang, Zhigang Zhang, Zhaoyuan Wu, Liqiao Shi, Fang Liu, Zhongyi Wan, Manli Liuantibody (ab3534), goat anti-rabbit IgG H&L (Alexa Fluor 488) (ab150077), and DAPI staining solution (ab228549) were purchased from Abcam. Cell Proliferation The supernatant was discarded upon Marc proliferation;

(169) Lumican silencing ameliorates β-glycerophosphate-mediated vascular smooth muscle cell calcification by attenuating the inhibition of APOB on KIF2C activityOpen medicine (Warsaw, Poland)January 1, 2023Li H, Zhang C, Liu Q.Fluor® 488 (ab150077; Abcam) and Alexa Fluor® 647 (ab150115; Abcam). Nuclei were labeled with DAPI (ab228549; Abcam). Protein images were captured using Zeiss LSM 710 confocal microscope (Carl Zeiss AG, Oberkochen,

(170) Intestinal epithelial stem cell transplants as a novel therapy for cerebrovascular strokeBrain, behavior, and immunityJanuary 1, 2023Mani KK, El-Hakim Y, Branyan TE, Samiya N, Pandey S, Grimaldo MT, Habbal A, Wertz A, Sohrabji F.in PBS, and cover slipped with mounting media containing the nuclear dye DAPI (Fluoroshield, Abcam, ab228549). Sections were imaged on the FV12-IX83 confocal microscope. 2.16. Villin, Ki67 quantification Gut

(171) Macrophages induce gingival destruction via Piezo1-mediated MMPs-degrading collagens in periodontitisFrontiers in immunologyJanuary 1, 2023Zhao T, Chu Z, Chu CH, Dong S, Li G, Wu J, Tang C.hour at room temperature under protection from light. Next, add 4’,6-diamidino-2-phenylindole (DAPI) (ab228549; Abcam, USA) to stain the nuclei and incubate for 10 minutes at room temperature in the dark. Finally,

(172) Characterizing nuclear morphology and expression of eNOS in vascular endothelial cells subjected to a continuous range of wall shear stress magnitudes and directionalityJournal of the Mechanical Behavior of Biomedical MaterialsJanuary 1, 2023Jaideep Sahni, Mehwish Arshad, Morgan A. Schake, Justin R. Brooks, Ruiguo Yang, Peter D. Weinberg, Ryan M. Pedrigi(ab150117, Abcam) at 1:700 for 1 h at room temperature. Cells were incubated with 10 μM DAPI nuclear stain (ab228549, Abcam) for 2 min. All antibodies were diluted in blocking buffer and incubation steps were conducted

(173) The mitochondrial Ca(2+) channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationFrontiers in cell and developmental biologyJanuary 1, 2023Fernandez Garcia E, Paudel U, Noji MC, Bowman CE, Rustgi AK, Pitarresi JR, Wellen KE, Arany Z, Weissenrieder JS, Foskett JK.Scientific, R37174), according to manufacturer’s instructions. After 15-min incubation, 4 mM DAPI (Abcam, ab228549) was added to the suspension and cells were analyzed by FACS with emission filters 530 and 440 nm for

(174) Parkia speciosa Hassk. Empty Pod Extract Prevents Cardiomyocyte Hypertrophy by Inhibiting MAPK and Calcineurin-NFATC3 Signaling PathwaysLife (Basel, Switzerland)December 23, 2022Mustafa NH, Jalil J, Saleh MSM, Zainalabidin S, Asmadi AY, Kamisah Y.and rinsed with PBS. The cellular nuclei were stained blue with 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam, Cambridge, MA, USA) and analyzed under a fluorescence microscope (Olympus Optical, Tokyo, Japan). 2.11.

(175) Gabapentin Increases Intra-Epidermal and Peptidergic Nerve Fibers Density and Alleviates Allodynia and Thermal Hyperalgesia in a Mouse Model of Acute Taxol-Induced Peripheral NeuropathyBiomedicinesDecember 8, 2022Michal Klazas, Majdi Saleem Naamneh, Wenhua Zheng, Philip Lazarovicigene-related peptides-I/II (CGRP-I/II) antibody [EPR23804-95] (ab283568) and DAPI staining solution (ab228549) were purchased from Abcam, Cambridge, UK). Alexa Fluor 594 affinity pure donkey (red, 112-585-167)

(176) Chronic Intermittent Hypoxia during Sleep Causes Browning of Interscapular Adipose Tissue Accompanied by Local Insulin Resistance in MiceInternational Journal of Molecular SciencesDecember 7, 2022Tehila Dahan, Shahd Nassar, Olga Yajuk, Eliana Steinberg, Ofra Benny, Nathalie Abudi, Inbar Plaschkes, Hadar Benyamini, David Gozal, Rinat Abramovitch, et al.MO, USA), Alexa Fluor®647 phalloidin (#ab176759, Invitrogen, Waltham, MA, USA), and DAPI (1:10,000, #ab228549, Abcam, Cambridge, UK). 4.6. Western Blot Analysis Tissues were homogenized in lysis buffer with protease

(177) In vitro neurons learn and exhibit sentience when embodied in a simulated game-worldNeuronDecember 7, 2022Brett J. Kagan, Andy C. Kitchen, Nhi T. Tran, Forough Habibollahi, Moein Khajehnejad, Bradyn J. Parker, Anjali Bhat, Ben Rollo, Adeel Razi, Karl J. FristonUSA) were incubated for 2 h. This was followed by 10 min of DAPI Staining Solution in PBS (1:1000, ab228549, Abcam, Cambridge, MA, USA) after which point slides were cover-slipped with ProLong Gold Antifade Mountant

(178) Spatial Analysis of Neural Cell Proteomic Profiles Following Ischemic Stroke in Mice Using High-Plex Digital Spatial ProfilingMolecular NeurobiologyDecember 1, 2022Jessica M. Noll, Catherine J. Augello, Esra KÃ¼rÃ¼m, Liuliu Pan, Anna Pavenko, Andy Nam, Byron D. Fordfor 2 min. Sections were then incubated in a freshly prepared solution of 0.0004% FJB with DAPI (Cat#ab228549, Abcam, Waltham, MA) for 20 min, rinsed in distilled water 3 times for 2 min, and then dried at 50 °C. For

(179) A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabricationBioactive materialsDecember 1, 2022Zhang Z, Wu C, Dai C, Shi Q, Fang G, Xie D, Zhao X, Liu YJ, Wang CCL, Wang XJ.then incubated with the secondary antibodies for 1 h at RT, counterstained with 0.5 μM DAPI (Abcam #ab228549) for 15 min at RT, and washed with 1 × PBS for 3 times. Coverslips and slides were mounted using Prolong Gold Antifade Reagent

(180) Aptamer engineering exosomes loaded on biomimetic periosteum to promote angiogenesis and bone regeneration by targeting injured nerves via JNK3 MAPK pathwayMaterials Today BioDecember 1, 2022Yanlin Su, Qing Gao, Rongli Deng, Lian Zeng, Jingyi Guo, Bing Ye, Jialin Yu, Xiaodong Guoantibodies (8889S, Cell Signaling Technology, Beverly, MA, USA) for 1 h. After staining with DAPI (ab228549, Abcam, USA) for 5 min and observed with a fluorescent microscope (IX73, Olympus, Tokyo, Japan). Quantitative

Write a Review

(1)

6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

Abcam

Citations (276)

Reviews

DAPI Staining Solution from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map