DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

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Website: http://www.abcam.com

(81) Prion protein regulates invasiveness in glioblastoma stem cellsBMC cancerDecember 18, 2024Prado MB, Coelho BP, Iglesia RP, Alves RN, Boccacino JM, Fernandes CFL, Melo-Escobar MI, Ayyadhury S, Cruz MC, Santos TG, et al.anti-mouse (#A28175, Invitrogen) secondary antibodies at a 1:1000 dilution, along with DAPI (Abcam, ab228549) at 1:1000, all in PBS with 2% normal goat serum and 0.2% Triton X-100. Coverslips were then washed

(82) Wanted: Dead or Alive Cells with Propidium Iodide Staining in Liver TissueInternational Journal of Molecular SciencesDecember 17, 2024Tim Christopher Krapoth, Gina Sophie Henle, Mihrije Avdyli, Berina Bektić, Katharina Maria Schwarzkopf, Larisa Bešić, Stefan Zeuzem, Christoph Welsch, Nico Kraus, Cristina OrtizResource,Source,Location,Identifier "4’,6-Diamidin-2-phenylindol (DAPI)",Abcam,"Campbridge, United Kingdom",Ab228549 Acetone p.a.,Sigma-Aldrich,"Saint Louis, MO, USA",32201-1L Alexa Fluor 488 phalloidin,Invitrogen,"Carlsbad,

(83) Phospho-regulation of ASCL1-mediated chromatin opening during cellular reprogrammingDevelopmentDecember 15, 2024Roberta Azzarelli, Sarah Gillen, Frances Connor, Jethro Lundie-Brown, Francesca Puletti, Rosalind Drummond, Ana Raffaelli, Anna PhilpottTriton X100-PBS containing 2% serum. Cells were stained with 1 μg/ml DAPI staining solution (Abcam, ab228549) for 20 min at room temperature. Imaging and image analysis Fluorescent images were taken using the

(84) Development of a fibrin-targeted theranostic for gastric cancerScience Translational MedicineDecember 11, 2024Shadi A. Esfahani, Li Ma, Shriya Krishna, Hua Ma, Shvan J. Raheem, Sergey Shuvaev, Nicholas J. Rotile, Jonah Weigand-Whittier, Avery T. Boice, Nicholas Borges, et al.Cell Signaling Technology) at 1/1000 dilution and for nuclei using 4′,6-diamidino-2-phenylindole (DAPI) staining solution (ab228549, Abcam). Microscopic evaluation was performed with a BioTeK Citation 5 microscopy system. The biomarker

(85) Fullerenol C60(OH)40 Nanoparticles and Ectoine Protect Human Nasal Epithelial Cells Against the Cytokine Storm After Addition of the Full-Length Spike Protein from SARS-CoV-2International Journal of NanomedicineNovember 21, 2024Malwina Sosnowska, Mateusz Wierzbicki, Barbara Nasiłowska, Totka Nikolaeva Bakalova, Klara Piotrowska, Barbara Strojny-Cieślak, Ewa Sawosz, Marta Kutwinwere washed three times with PBS and then stained with 5 µM 4′,6-diamidino-2-phenylindole (DAPI, No. ab228549, Abcam) for 15 min. After rinsing with PBS, the Ibidi Mounting Medium (No. 50001, Animalab, Teltow,

(86) Hierarchically Structured and Tunable Hydrogel Patches: Design, Characterization, and ApplicationSmallNovember 20, 2024Lea Steinbeck, Richard Paul, Julia Litke, Isabel Karkoszka, G. Philip Wiese, John Linkhorst, Laura De Laporte, Matthias WesslingPBS (1X, 150 μL), before staining (5 min) with 4',6‐diamidino‐2‐phenylindole (DAPI) (0.4 v%, 150 μL, ab228549, Abcam). Wells were washed two times with PBS (1X, 200 μL) and stored at 4 °C under the exclusion of

(87) Dental pulp stem cells promote genioglossus repair and systemic amelioration in chronic intermittent hypoxiaiScienceNovember 15, 2024Meng-Han Zhang, Wei-Hua Zhang, Yun Lu, Li-Ming Yu, Xin-Xin Han, Yan Xu, Meng-Jie Wu, Wang-Hui Ding, Yue-Hua LiuBovine Serum Albumin (BSA),Solarbio,9048-46-8 RIPA lysis buffer,Thermo Fisher,89900 protease/phosphatase inhibitor cocktail,CST,5872 DAPI,Abcam,ab228549 BD Vacutainer® SST™ Tubes,BD Vacutainer,367957 alanine aminotransferase (ALT),Changchun Huili,C052-day aspartate

(88) Cell Adhesion and Local Cytokine Control on Protein‐Functionalized PNIPAM‐co‐AAc Hydrogel MicrocarriersSmallNovember 13, 2024Sebastian Bernhard Rauer, Lucas Stüwe, Lea Steinbeck, Marcelo Augusto Szymanski de Toledo, Gereon Fischer, Simon Wennemaring, Jonas Marschick, Steffen Koschmieder, Matthias Wessling, John Linkhorstplasma membranes by exposing the specimen to 4:1000 v/v 4,6‐diamidino‐2‐phenylindole (DAPI) solution (Abcam, ab228549) for 10 min and 1:1000 v/v CellMaskTM Orange (Thermofisher Scientific, C10045) for 30 min. Prior to

(89) Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable ScaffoldsAdvanced Healthcare MaterialsNovember 12, 2024Magdalena Z. Gładysz, Didi Ubels, Marcus Koch, Armin Amirsadeghi, Frederique Alleblas, Sander van Vliet, Marleen Kamperman, Jeroen Siebring, Anika Nagelkerke, Małgorzata K. Włodarczyk‐Biegundilution) for 90 min. Then the samples were washed three times with DPBS and incubated with DAPI (Abcam, ab228549, Netherlands) solution in DPBS (1:1000 dilution) for 10 min. Lastly, the samples were washed three times

(90) Soft‐Actuated Cuff Electrodes with Minimal Contact for Bidirectional Peripheral InterfacesAdvanced MaterialsNovember 10, 2024Hyunmin Moon, Byungwook Park, Namsun Chou, Ki‐Su Park, Sanghoon Lee, Sohee Kim(A11126, Abcam; 1:200 dilution). At the same time, the sample was nuclear counterstained using DAPI (ab228549, Abcam; 1:1000 dilution). Then, the sample was washed four times for 5 min with PBS and mounted using

(91) Single-cell RNA sequencing reveals the process of CA19-9 production and dynamics of the immune microenvironment between CA19-9 (+) and CA19-9 (-) PDACChinese medical journalOctober 20, 2024Zhang D, Cui F, Zheng K, Li W, Liu Y, Wu C, Peng L, Yang Z, Chen Q, Xia C, et al.incubated with multiple antibodies and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (catalogue #ab228549, Abcam, Cambridge, UK). Finally, the slides were observed under a fluorescence microscope. Flow cytometry analysis

(92) Platelet factors ameliorate thoracic aortic aneurysm and dissection by inhibiting the FGF-FGFR cascade activation in aortic-endothelial celliScienceOctober 18, 2024Jizhong Wang, Caiyun He, Yuanwei Chen, Xiaolu Hu, Heng Xu, Jie Liu, Yi Yang, Lang Chen, Ting Li, Lixin Fang, et al.room temperature for 1 h. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, ab228549, UK). Images were acquired using a confocal microscope. Gelatin zymography To analyze the activity

(93) Effects of oxytocin receptor agonists on hair growth promotionScientific ReportsOctober 13, 2024Tatsuto Kageyama, Jieun Seo, Lei Yan, Junji Fukuda°C and lastly with rhodamine-phalloidin (ab235138, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) in PBS for 30 min. A confocal microscope (LSM 700, Carl Zeiss, Germany) was used for fluorescence imaging

(94) Tau is required for glial lipid droplet formation and resistance to neuronal oxidative stressNature NeuroscienceOctober 1, 2024Lindsey D. Goodman, Isha Ralhan, Xin Li, Shenzhao Lu, Matthew J. Moulton, Ye-Jin Park, Pinghan Zhao, Oguz Kanca, Ziyaneh S. Ghaderpour Ghaderpour Taleghani, Julie Jacquemyn, et al.(Cytiva, no. SH30268.01) for 4 h at 37 °C. Glia were then fixed in 4% PFA, stained with DAPI (Abcam, no. ab228549) and mounted using DAKO fluorescence mounting media (Agilent, no. S302380–2). For LD imaging using HSC

(95) Choroid plexus CCL2‒CCR2 signaling orchestrates macrophage recruitment and cerebrospinal fluid hypersecretion in hydrocephalusActa Pharmaceutica Sinica BOctober 1, 2024Qiguang Wang, Fei Liu, Yue Li, Huan Zhang, Xin Qi, Ke Wu, Yi Zhang, Shenglan You, Wenke Liu, Xuhui Hui, et al.anti-rabbit Alexa 550-conjugated IgG (Jackson, 111-025-003). Then the sections were stained with DAPI (Abcam, ab228549) after washing 3 times in PBS at RT. At last, sections were mounted with an anti-fade fluorescence mounting

(96) The destruction of cytoplasmic skeleton leads to the change of nuclear structure and the looseness of lamin A submicroscopic networkHeliyonSeptember 30, 2024Zhenyu Yang, Xianglong Liu, Xiaoliang Li, Maurizio Abbate, Han Rui, Miao Guan, Zhenglong Sunwere washed three times with PBS for 20 min. The labeled cells were stained with DAPI (Abcam, Cat# ab228549) for 15 min, washed three times with PBS for 5 min and stored at 4 °C or imaged immediately. 4.3 Microscopy

(97) Dysregulated miR-124 mediates impaired social memory behavior caused by paternal early social isolationTranslational psychiatrySeptember 28, 2024Chen S, Ding S, Pang Y, Jin Y, Sun P, Li Y, Cao M, Wang Y, Wang Z, Wang T, et al.and #A31570) for 2 hours at 25°C. The nuclei were stained by DAPI staining solution (1:1000; Abcam, #ab228549) for 10 minutes at 25°C. Western blot Frozen mPFC, hippocampal and amygdala brain tissues were lysed

(98) FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretionLife Science AllianceSeptember 1, 2024Kritika Saxena, Katharina Inholz, Michael Basler, Annette AichemX-100/PBS. After two washing steps, A549 cells stably expressing GFP and/or mCherry were stained with DAPI (ab228549, 1:1,000; Abcam) and directly mounted on glass slides using Fluoromount-G mounting media (0100-01; Southern biotech

(99) Estrogen-dependent gene regulation: Molecular basis of TIMP-1 as a sex-specific biomarker for acute lung injuryPhysiological reportsSeptember 1, 2024Almuntashiri S, Dutta S, Zhu Y, Gamare S, Ramírez G, Irineo-Moreno V, Camarena A, Regino N, Campero P, Hernández-Cardenas CM, et al.primary antibodies. Cells or lung sections were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (ab228549, Abcam, Cambridge, MA). All digital images were visualized at room temperature using a Zeiss Observer

(100) Genome-wide CRISPR-Cas9 knockout screens identify DNMT1 as a druggable dependency in sonic hedgehog medulloblastomaActa Neuropathologica CommunicationsAugust 7, 2024Foteini Tsiami, Chiara Lago, Noemi Pozza, Federica Piccioni, Xuesong Zhao, Fabienne Lülsberg, David E. Root, Luca Tiberi, Marcel Kool, Jens Schittenhelm, et al.Thermofisher Scientific, A11035) for 1h at room temperature. Nuclei were counterstained with DAPI 10 mM (Abcam, ab228549). Sections and coverslips (Thermofisher Scientific, 15,747,592) were mounted with permanent mounting

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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