DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Website: http://www.abcam.com

(121) Cellular and molecular characterization of peripheral glia in the lung and other organsPloS oneJanuary 1, 2024Hall S, Liu S, Liang I, Schulz S, Ezran C, Tan M, Kuo CS.antibodies (Table 2). Sections were incubated with 4′, 6-diamidino-2-phenylindole (DAPI) (1:10,000, ab228549; Abcam, USA) for 1hr, fixed for 1h in 4% paraformaldehyde, washed with PBS, and placed in Cubic 1 solution

(122) The effect of human PBMCs immobilization on their Аβ42 aggregates-dependent proinflammatory state on a cellular model of Alzheimer''s diseaseFrontiers in neuroscienceJanuary 1, 2024Kot K, Kot Y, Kurbanov R, Andriiash H, Tigunova O, Blume Y, Shulga S.cell morphology was assessed by confocal microscopy after staining nuclear DNA with DAPI dye (Abcam, ab228549, λEx – 405 nm, λEm = 461 nm). The hPBMCs studied included all mononuclear blood cells, such as lymphocytes,

(123) Biological and clinical significance of the AGE-RAGE axis in the aggressiveness and prognosis of prostate cancerJournal of food and drug analysisDecember 15, 2023Khoo SH, Wu PR, Yeh KT, Hsu SL, Wu CH.Scientific Hoechst 33342,62249,Thermo Fisher Propidium iodide (PI),P3566,Thermo Fisher Scientific DAPI,ab228549,Abcam HMGB1 recombinant protein,ab167718,Abcam Listed of chemicals used in this study

(124) Systematic assessment of transcriptomic and metabolic reprogramming by blue light exposure coupled with agingPNAS NexusDecember 5, 2023Jia Huang, Fan Zhou, Huanchan Zhou, Xiaoqi Zheng, Zhengyi Huo, Meng Yang, Zihe Xu, Runzhou Liu, Luoluo Wang, Xiaoyun Wang(#A-11008, Invitrogen) for 1 h. Tissues were mounted in PBT buffer and stained with DAPI Staining Solution (#ab228549, Abcam). A FLUOVIEW FV3000 confocal laser scanning microscope (Olympus, Shinjuku, Japan) was used to

(125) Single-cell profiling reveals transcriptomic signatures of vascular endothelial cells in non-healing diabetic foot ulcersFront Endocrinol (Lausanne)December 1, 2023Yangzhou Lu, Xiaogang Liu, Jingling Zhao, Fan Bie, Yiling Liu, Julin Xie, Peng Wang, Junyou Zhu, Yahui Xiong, Shitian Qin, et al.Biosciences,Servicebio,Servicebio,Abcam Catalog Number,AF6191,AF0931,DF6191,AF1009,AF5176,S0001,G1222,G1223,ab228549 Dilution,1:200,1:200,1:100,1:200,1:100,1:200,1:500,1:500,1:10000 Serum sPD-L1 changes before and

(126) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environmentMatrix Biology PlusDecember 1, 2023Lisa J. Neilson, Douglas Cartwright, Maija Risteli, Elina M. Jokinen, Lynn McGarry, Toni Sandvik, Konstantina Nikolatou, Kelly Hodge, Samuel Atkinson, Maria Vias, et al.at 1:500 in 1 % BSA/0.1 % saponin in PBS for 1 h RT. Following a wash in 1 % BSA in PBS, Dapi (Abcam ab228549) at 1:2000 in 1 % BSA in PBS was applied for 20 min at room temperature. After a final wash in 1 % BSA

(127) Influence of metabolic stress and metformin on synaptic protein profile in SH‐SY5Y‐derived neuronsPhysiological ReportsNovember 27, 2023Alex J. T. Yang, Ahmad Mohammad, Michael S. Finch, Evangelia Tsiani, Gaynor Spencer, Aleksandar Necakov, Rebecca E. K. MacPhersonbuffer containing phalloidin actin stain (Abcam; ab176757 (Wang et al., 2022)) and DAPI stain (Abcam; ab228549). Images were captured using a Biotek Cytation5 cell imaging reader. Immunofluorescent (IF) images were

(128) INO80 function is required for mouse mammary gland development, but mutation alone may be insufficient for breast cancerFrontiers in Cell and Developmental BiologyNovember 1, 2023Nguyen Xuan Thang, Dong Wook Han, Chanhyeok Park, Hyeonji Lee, Hyeonwoo La, Seonho Yoo, Heeji Lee, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, et al.Abcam)] in the dark for 1 h, washed with 1X PBS thrice, and briefly counter-stained with DAPI (1:1000, #ab228549; Abcam). The sections were mounted and imaged using a confocal microscope (LSM800; Carl Zeiss, Oberkochen,

(129) Follicle-stimulating hormone orchestrates glucose-stimulated insulin secretion of pancreatic isletsNature CommunicationsNovember 1, 2023Yi Cheng, Hong Zhu, Jun Ren, Hai-Yan Wu, Jia-En Yu, Lu-Yang Jin, Hai-Yan Pang, Hai-Tao Pan, Si-Si Luo, Jing Yan, et al.fluorescent-conjugated secondary antibodies (1:500; Invitrogen, A21241, A11034). Nuclei were stained with DAPI (Abcam, ab228549). All immunofluorescence images were acquired by laser scanning confocal microscope (Zeiss). RNA interference Small

(130) Effects of oxytocin on the hair growth ability of dermal papilla cellsScientific ReportsOctober 20, 2023Tatsuto Kageyama, Jieun Seo, Lei Yan, Junji Fukuda°C and lastly with rhodamine-phalloidin (ab235138, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) in PBS for 30 min. A confocal microscope (LSM 700; Carl Zeiss, Germany) was used for fluorescence imaging

(131) Radiotherapy-Induced Neurocognitive Impairment Is Driven by Heightened Apoptotic Priming in Early Life and Prevented by Blocking BAXCancer researchOctober 13, 2023Singh R, Yu S, Osman M, Inde Z, Fraser C, Cleveland AH, Almanzar N, Lim CB, Joshi GN, Spetz J, et al.incubation at 1:200 dilution (Thermo Fisher Scientific A-11008, RRID:AB_143165) and DAPI staining (Abcam ab228549) was performed the next day, with cover slipping using vector labs antifade mounting media (Vector Laboratories

(132) Small Molecule Pytren-4QMn Metal Complex Slows down Huntington’s Disease Progression in Male zQ175 Transgenic MiceInternational Journal of Molecular SciencesOctober 13, 2023Marián Merino, Sonia González, Mª Carmen Tronch, Ana Virginia Sánchez-Sánchez, Mª Paz Clares, Antonio García-España, Enrique García-España, José L. Mullorantibody Alexa Fluor® 488 (ab150113; Abcam, Cambridge, UK) diluted 1:500 in blocking solution and DAPI (ab228549; Abcam, Cambridge, UK). Finally, the samples were washed three times with PBS 1× and assembled with

(133) Palbociclib releases the latent differentiation capacity of neuroblastoma cellsDevelopmental CellOctober 9, 2023Kirsty M. Ferguson, Sarah L. Gillen, Lewis Chaytor, Evon Poon, Daniel Marcos, Roshna Lawrence Gomez, Laura M. Woods, Lidiya Mykhaylechko, Louis Elfari, Barbara Martins Martins da Costa, et al.BioReagents™,BP9706-100 Beta-mercaptoethanol,BDH,441433A Trypsin EDTA 0.25%,ThermoFisher Scientific,25200072 DAPI,Abcam,ab228549 DMSO,Santa Cruz Biotechnology,sc-358801 PowerUp™ SYBR™ Green Master Mix,ThermoFisher Scientific,A25742 Crystal

(134) Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFAJournal of nanobiotechnologyOctober 3, 2023Zhang S, Huang Y, Pi S, Chen H, Ye F, Wu C, Li L, Ye Q, Lin Y, Su Z.Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077), Goat anti-rabbit IgG H&L (Alexa Fluor® 555) (ab150078), and DAPI Staining Solution (ab228549) from Abcam. The preparation of STF@ZIF-8/PEG-FA (SZP), BMS@ZIF-8/PEG-FA(BZP), and STF-BMS@ZIF-8/PEG-FA

(135) Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 RevStructureOctober 1, 2023Elif Eren, Norman R. Watts, Davide Randazzo, Ira Palmer, Dan L. Sackett, Paul T. Wingfield11873580001 Nocodazole,Sigma-Aldrich,Cat# M1404-2MG Taxol (paclitaxel),Invitrogen,Cat# P3456 DAPI staining solution,Abcam,Cat# ab228549 Lipofectamine 3000,Invitrogen,Cat# L3000015 Opti-MEM,Gibco,Cat# 31985070 Subtilisin,Hampton Research

(136) Loss of Grin2a causes a transient delay in the electrophysiological maturation of hippocampal parvalbumin interneuronsCommunications BiologySeptember 19, 2023Chad R. Camp, Anna Vlachos, Chiara Klöckner, Ilona Krey, Tue G. Banke, Nima Shariatzadeh, Sarah M. Ruggiero, Peter Galer, Kristen L. Park, Adam Caccavano, et al.temperature. Slices were washed 3 times in PBS for 10 min then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 min before being washed 3 more times in PBS for 10 min. Slices were mounted on Superfrost

(137) Microglial SIRT1 activation attenuates synapse loss in retinal inner plexiform layer via mTORC1 inhibitionJournal of neuroinflammationSeptember 5, 2023Yao K, Mou Q, Lou X, Ye M, Zhao B, Hu Y, Luo J, Zhang H, Li X, Zhao Y.anti-Iba1 antibody (ab5076 and ab178846; Abcam; 1:200); anti-CD68 antibody (ab955; Abcam; 1:50); DAPI (ab228549; Abcam; 1:1000); anti-TSC2 antibody (#4308; cell signaling technology; 1:800); anti-HA-Tag antibody

(138) Curcumin-activated Olfactory Ensheathing Cells Improve Functional Recovery After Spinal Cord Injury by Modulating Microglia Polarization Through APOE/TREM2/NF-κB Signaling PathwayJournal of Neuroimmune PharmacologySeptember 2, 2023Chao Jiang, Zhe Chen, Xiaohui Wang, Yongyuan Zhang, Xinyu Guo, Hong Fan, Dageng Huang, Yuqing He, Xiangwen Tang, Yixiang Ai, et al.1:200, Abcam), pNF-κB (3033, 1:200, Cell Signaling Technology), APOE (ab183597, 1:200, Abcam) and DAPI (ab228549, 1:1000, Abcam). After incubation with the corresponding secondary antibodies, coverslips were washed

(139) Self-oxygenation of engineered living tissues orchestrates osteogenic commitment of mesenchymal stem cellsBiomaterialsSeptember 1, 2023Shabir Hassan, Ting Wang, Kun Shi, Yike Huang, Maria Elizabeth Urbina Lopez, Kaifeng Gan, Mo Chen, Niels Willemen, Haroon Kalam, Eder Luna-Ceron, et al.(Catalog No.: ab113851), primary antibodies for F-actin (Catalog No.: ab205) and DAPI (Catalog No.: ab228549) were purchased from Abcam and used as per the manufacturer’s protocol. Recombinant human fibroblast

(140) Long-term survival of LGR5 expressing supporting cells after severe ototoxic trauma in the adult mouse cochleaFrontiers in Cellular NeuroscienceAugust 24, 2023Natalia Smith-Cortinez, Ferry G. J. Hendriksen, Dyan Ramekers, Robert J. Stokroos, Huib Versnel, Louise V. StraatmanInvitrogen, A-21207), donkey-anti Goat-Alexa 488 (1/500, Abcam, AB150129), and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Lastly, specimens were washed in PBS and mounted in Vectashield Antifade

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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