DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(101) Melt Electrowriting of Polyhydroxyalkanoates for Enzymatically Degradable ScaffoldsAdvanced Healthcare MaterialsNovember 12, 2024Magdalena Z. Gładysz, Didi Ubels, Marcus Koch, Armin Amirsadeghi, Frederique Alleblas, Sander van Vliet, Marleen Kamperman, Jeroen Siebring, Anika Nagelkerke, Małgorzata K. Włodarczyk‐Biegundilution) for 90 min. Then the samples were washed three times with DPBS and incubated with DAPI (Abcam, ab228549, Netherlands) solution in DPBS (1:1000 dilution) for 10 min. Lastly, the samples were washed three times

(102) Soft‐Actuated Cuff Electrodes with Minimal Contact for Bidirectional Peripheral InterfacesAdvanced MaterialsNovember 10, 2024Hyunmin Moon, Byungwook Park, Namsun Chou, Ki‐Su Park, Sanghoon Lee, Sohee Kim(A11126, Abcam; 1:200 dilution). At the same time, the sample was nuclear counterstained using DAPI (ab228549, Abcam; 1:1000 dilution). Then, the sample was washed four times for 5 min with PBS and mounted using

(103) Single-cell RNA sequencing reveals the process of CA19-9 production and dynamics of the immune microenvironment between CA19-9 (+) and CA19-9 (-) PDACChinese medical journalOctober 20, 2024Zhang D, Cui F, Zheng K, Li W, Liu Y, Wu C, Peng L, Yang Z, Chen Q, Xia C, et al.incubated with multiple antibodies and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (catalogue #ab228549, Abcam, Cambridge, UK). Finally, the slides were observed under a fluorescence microscope. Flow cytometry analysis

(104) Platelet factors ameliorate thoracic aortic aneurysm and dissection by inhibiting the FGF-FGFR cascade activation in aortic-endothelial celliScienceOctober 18, 2024Jizhong Wang, Caiyun He, Yuanwei Chen, Xiaolu Hu, Heng Xu, Jie Liu, Yi Yang, Lang Chen, Ting Li, Lixin Fang, et al.room temperature for 1 h. Cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (Abcam, ab228549, UK). Images were acquired using a confocal microscope. Gelatin zymography To analyze the activity

(105) Effects of oxytocin receptor agonists on hair growth promotionScientific ReportsOctober 13, 2024Tatsuto Kageyama, Jieun Seo, Lei Yan, Junji Fukuda°C and lastly with rhodamine-phalloidin (ab235138, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) in PBS for 30 min. A confocal microscope (LSM 700, Carl Zeiss, Germany) was used for fluorescence imaging

(106) Tau is required for glial lipid droplet formation and resistance to neuronal oxidative stressNature NeuroscienceOctober 1, 2024Lindsey D. Goodman, Isha Ralhan, Xin Li, Shenzhao Lu, Matthew J. Moulton, Ye-Jin Park, Pinghan Zhao, Oguz Kanca, Ziyaneh S. Ghaderpour Ghaderpour Taleghani, Julie Jacquemyn, et al.(Cytiva, no. SH30268.01) for 4 h at 37 °C. Glia were then fixed in 4% PFA, stained with DAPI (Abcam, no. ab228549) and mounted using DAKO fluorescence mounting media (Agilent, no. S302380–2). For LD imaging using HSC

(107) Choroid plexus CCL2‒CCR2 signaling orchestrates macrophage recruitment and cerebrospinal fluid hypersecretion in hydrocephalusActa Pharmaceutica Sinica BOctober 1, 2024Qiguang Wang, Fei Liu, Yue Li, Huan Zhang, Xin Qi, Ke Wu, Yi Zhang, Shenglan You, Wenke Liu, Xuhui Hui, et al.anti-rabbit Alexa 550-conjugated IgG (Jackson, 111-025-003). Then the sections were stained with DAPI (Abcam, ab228549) after washing 3 times in PBS at RT. At last, sections were mounted with an anti-fade fluorescence mounting

(108) The destruction of cytoplasmic skeleton leads to the change of nuclear structure and the looseness of lamin A submicroscopic networkHeliyonSeptember 30, 2024Zhenyu Yang, Xianglong Liu, Xiaoliang Li, Maurizio Abbate, Han Rui, Miao Guan, Zhenglong Sunwere washed three times with PBS for 20 min. The labeled cells were stained with DAPI (Abcam, Cat# ab228549) for 15 min, washed three times with PBS for 5 min and stored at 4 °C or imaged immediately. 4.3 Microscopy

(109) Dysregulated miR-124 mediates impaired social memory behavior caused by paternal early social isolationTranslational psychiatrySeptember 28, 2024Chen S, Ding S, Pang Y, Jin Y, Sun P, Li Y, Cao M, Wang Y, Wang Z, Wang T, et al.and #A31570) for 2 hours at 25°C. The nuclei were stained by DAPI staining solution (1:1000; Abcam, #ab228549) for 10 minutes at 25°C. Western blot Frozen mPFC, hippocampal and amygdala brain tissues were lysed

(110) FAT10 inhibits TRIM21 to down-regulate antiviral type-I interferon secretionLife Science AllianceSeptember 1, 2024Kritika Saxena, Katharina Inholz, Michael Basler, Annette AichemX-100/PBS. After two washing steps, A549 cells stably expressing GFP and/or mCherry were stained with DAPI (ab228549, 1:1,000; Abcam) and directly mounted on glass slides using Fluoromount-G mounting media (0100-01; Southern biotech

(111) Estrogen-dependent gene regulation: Molecular basis of TIMP-1 as a sex-specific biomarker for acute lung injuryPhysiological reportsSeptember 1, 2024Almuntashiri S, Dutta S, Zhu Y, Gamare S, Ramírez G, Irineo-Moreno V, Camarena A, Regino N, Campero P, Hernández-Cardenas CM, et al.primary antibodies. Cells or lung sections were counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (ab228549, Abcam, Cambridge, MA). All digital images were visualized at room temperature using a Zeiss Observer

(112) Genome-wide CRISPR-Cas9 knockout screens identify DNMT1 as a druggable dependency in sonic hedgehog medulloblastomaActa Neuropathologica CommunicationsAugust 7, 2024Foteini Tsiami, Chiara Lago, Noemi Pozza, Federica Piccioni, Xuesong Zhao, Fabienne Lülsberg, David E. Root, Luca Tiberi, Marcel Kool, Jens Schittenhelm, et al.Thermofisher Scientific, A11035) for 1h at room temperature. Nuclei were counterstained with DAPI 10 mM (Abcam, ab228549). Sections and coverslips (Thermofisher Scientific, 15,747,592) were mounted with permanent mounting

(113) Exclusion of sulfide:quinone oxidoreductase from mitochondria causes Leigh-like disease in mice by impairing sulfide metabolismJournal of Clinical InvestigationAugust 1, 2024Eiki Kanemaru, Kakeru Shimoda, Eizo Marutani, Masanobu Morita, Maria Miranda, Yusuke Miyazaki, Claire Sinow, Rohit Sharma, Fangcong Dong, Donald B. Bloch, et al.diluted 1:500 in double-salt PBS containing Triton X-100 (0.3%). The sections were stained with DAPI (ab228549, Abcam) before mounting with Fluoromount-G medium (0100-01, Southern Biotech). The stained sections

(114) Antibody–Drug Conjugate Sacituzumab Govitecan Enables a Sequential TOP1/PARP Inhibitor Therapy Strategy in Patients with Breast CancerClinical Cancer ResearchJuly 15, 2024Aditya Bardia, Sheng Sun, Nayana Thimmiah, James T. Coates, Bogang Wu, Rachel O. Abelman, Laura Spring, Beverly Moy, Phoebe Ryan, Mark N. Melkonyan, et al.for 1 hour. The cells were then washed twice with 2% BSA in PBS and counterstained with DAPI (Abcam, #ab228549). For flow cytometry detection of apoptotic cells, 1 × 106 cells were collected, and the staining was

(115) Pulsatile flow increases METTL14-induced m 6 A modification and attenuates septic cardiomyopathy: an experimental studyInternational journal of surgery (London, England)July 1, 2024Zhu S, Wang K, Yu Z, Tang W, Zhang Y, Shinge SA, Qiang Y, Liu H, Zeng J, Qiao K, et al.(ab6721; Abcam, Cambridge, MA, USA) was then added, followed by 4′,6-diamidino2-phenylindole (DAPI) (ab228549; Abcam, Cambridge, MA, USA) staining. For cellular immunofluorescence, CMECs were fixed with 4% paraformaldehyde

(116) Exploring the Link Between Autophagy‐Lysosomal Dysfunction and Early Heterotopic Ossification in TendonsAdvanced ScienceJuly 1, 2024Chang‐He Gao, Qian‐Qian Wan, Jan‐Fei Yan, Yi‐Na Zhu, Lei Tian, Jian‐Hua Wei, Bin Feng, Li‐Na Niu, Kai Jiaodilution for 1 h at 37 °C. The cell nuclei were stained using 4′,6‐diamidino‐2‐phenylindole (DAPI; ab228549; Abcam) at room temperature for 15 minutes. For Alizarin Red S staining, the frozen sections were stained

(117) Evaluating the pro-survival potential of apoptotic bodies derived from 2D- and 3D- cultured adipose stem cells in ischaemic flapsJournal of NanobiotechnologyJune 14, 2024Gaoxiang Yu, Jian Ding, Ningning Yang, Lu Ge, Nuo Chen, Xuzi Zhang, Qiuchen Wang, Xian Liu, Xuanlong Zhang, Xiaoqiong Jiang, et al.phalloidin-FITC (Actin-Tracker Green; Beyotime, C1033), and the nucleus was stained with DAPI (Abcam, ab228549). The Calcein/PI cell viability and cytotoxicity detection kit (C2015S) were employed, following the

(118) Exclusion of sulfide:quinone oxidoreductase from mitochondria causes Leigh-like disease in mice by impairing sulfide metabolismJ Clin InvestJune 13, 2024Eiki Kanemaru, Kakeru Shimoda, Eizo Marutani, Masanobu Morita, Maria Miranda, Yusuke Miyazaki, Claire Sinow, Rohit Sharma, Fangcong Dong, Donald B. Bloch, et al.diluted 1:500 in double-salt PBS containing Triton X-100 (0.3%). The sections were stained with DAPI (ab228549, Abcam) before mounting with Fluoromount-G medium (0100-01, Southern Biotech). The stained sections

(119) Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1Regenerative therapyJune 1, 2024Chen YT, Young TH, Wang YH, Huang CH, Gao YY, Huang TW.Plasma Membrane Stains (C10046, Thermo Fisher Scientific) and 4′,6-diamidino-2-phenylindole (DAPI) (ab228549, Abcam, Cambridge, UK) to demonstrate the cell morphology after 28 days of culture. For better visualization

(120) MiR-184 Mediated the Expression of ZNF865 in Exosome to Promote Procession in the PD ModelMolecular neurobiologyJune 1, 2024Liu C, Wang Y, Li JW, Zhu X, Jiang HS, Zhao H, Zhang LM.with a commercial TUNEL assay kit (Abcam; ab206386). Nuclei are stained with DAPI solution (Abcam; ab228549). There are three different fields for every section and four sections per group to analyze. Immunohistochemistry (IHC

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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