DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(141) Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic StressCellsAugust 14, 2023Meneses-San Juan D, Lamas M, Ramírez-Rodríguez GB.anti-5-methylcytosine (1:500, Cat: ab214727, Abcam). 4′,6-diamidino-2-phenylindole (DAPI), (1:1000, Cat: ab228549, Abcam) was used to stain the nuclei of the hippocampal dentate gyrus cells. Images were acquired using

(142) Identification of a novel enhancer essential for Satb1 expression in T(H)2 cells and activated ILC2sLife science allianceAugust 1, 2023Nomura A, Kobayashi T, Seo W, Ohno-Oishi M, Kakugawa K, Muroi S, Yoshida H, Endo TA, Moro K, Taniuchi I.kept at −20°C until analysis. On the day of the analysis, thymocytes were stained with 1/100 of DAPI (ab228549; Abcam) at room temperature, for 30 min, and washed three times in PBS. Cover slips were mounted on

(143) Integrated single‐cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial–fibroblast interactions in recurrent bladder cancerClinical and Translational MedicineJuly 24, 2023Zhen‐Duo Shi, Zhuo Sun, Zuo‐Bin Zhu, Xing Liu, Jun‐Zhi Chen, Lin Hao, Jie‐Fei Zhu, Kun Pang, Di Wu, Yang Dong, et al.ab75813), CCL22 anti‐human Ab (Abcam, ab23772) and 4′,6‐diamidino‐2‐phenylindole staining solution (Abcam, ab228549). Following the manufacturer's instructions (Akoya, Opal Polaris 7 Color Automation IHC Detection Kit),

(144) Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated StrainVirusesJuly 21, 2023Sok Song, Gyu-Nam Park, Jihye Shin, Ki-Sun Kim, Byung-Hyun An, SeEun Choe, Song-Yi Kim, Bang-Hun Hyun, Dong-Jun AnAfter washing, cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam; catalog no. ab228549) at room temperature, followed by observation under a fluorescence microscope (200×). 2.6. Virus Growth

(145) Single‐cell landscape analysis reveals systematic senescence in mammalian Down syndromeClinical and Translational MedicineJuly 17, 2023Yao Chen, Yanyu Xiao, Yanye Zhang, Renying Wang, Feixia Wang, Huajing Gao, Yifeng Liu, Runju Zhang, Huiyu Sun, Ziming Zhou, et al.times with 1% BSA in PBS, the slides were incubated with secondary antibodies (Thermo Fisher) and DAPI (ab228549; Abcam) for 1 h at room temperature. TUNEL staining was performed using a one‐step TUNEL Apoptosis Detection

(146) Early maturation and hyperexcitability is a shared phenotype of cortical neurons derived from different ASD-associated mutationsTranslational PsychiatryJuly 6, 2023Yara Hussein, Utkarsh Tripathi, Ashwani Choudhary, Ritu Nayak, David Peles, Idan Rosh, Tatiana Rabinski, Jose Djamus, Gad David Vatine, Ronen Spiegel, et al.solution overnight at 4 °C. On the next day, they were washed in DPBS and incubated with DAPI (Abcam, ab228549, 1:1000) and corresponding secondary antibodies (Abcam, ab150084, ab150117, ab175711, 1:250) for 60

(147) Immunohistologic Features of Cerebral Venous Thrombosis Due to Vaccine-Induced Immune Thrombotic ThrombocytopeniaNeurology(R) neuroimmunology & neuroinflammationJuly 1, 2023Geeraerts T, Guilbeau-Frugier C, Garcia C, Memier V, Raposo N, Bonneville F, Gales C, Darcourt J, Voisin S, Ribes A, et al.temperature. In some instances, wheat germ agglutinin was used. Sections were then stained with DAPI (Abcam, ab228549) for 15 minutes. Sections were labeled with secondary antibodies (Oregon-Green-488 goat antirabbit IgG,

(148) Restoration of normal blood flow in atherosclerotic arteries promotes plaque stabilizationiScienceJune 16, 2023Schake MA, McCue IS, Curtis ET, Ripperda TJ Jr, Harvey S, Hackfort BT, Fitzwater A, Chatzizisis YS, Kievit FM, Pedrigi RM.mg),Apotex Corp.,Cat# 60505-2579-09 Prolong Gold antifade reagent,Invitrogen,Cat#P36930 DAPI,Abcam,Cat#ab228549 Hydrochloric Acid,Sigma-Aldrich,Cat#32031-500ML Ethanol,Decon Laboratories Inc.,Cat#2701 Acetone,Fisher

(149) Chitosomes Loaded with Docetaxel as a Promising Drug Delivery System to Laryngeal Cancer Cells: An In Vitro Cytotoxic StudyInternational Journal of Molecular SciencesJune 8, 2023Christian R. Moya-Garcia, Nicole Y. K. Li-Jessen, Maryam Tabrizian(cat. # ab65393), ALEXA647/EGFR (cat. # ab192982), ALEXA488/TUBIII (cat. # ab195879), and DAPI (cat. # ab228549) from Abcam (Cambridge, UK) were used to visualize the cell membrane, cytoskeleton, and nucleus, respectively.

(150) Enhancement of Collagen-I Levels in Human Gingival Fibroblasts by Small Molecule Activation of HIF-1αJournal of Agricultural and Food ChemistryMay 24, 2023Lucia Adriana Lifshits, Miryam Rabin, Ran Tohar, Francesca Netti, Matan Gabay, Marina Sova, Daniel Z. Bar, Evgeny Weinberg, Lihi Adler-Abramovich, Maayan Gal(ab150077, Abcam)), Goat Anti-Mouse IgG H&L (Alexa Fluor 647) (ab150115, Abcam), and DAPI Staining Solution (ab228549, Abcam). Cellular viability was assessed using a live/dead viability assay (04511, Merck) and CellTiter

(151) Zika virus triggers autophagy to exploit host lipid metabolism and drive viral replicationCell Communication and SignalingMay 19, 2023Gloria Stoyanova, Sidra Jabeen, Joselyn Landazuri Vinueza, Sounak Ghosh Roy, Richard A. Lockshin, Zahra Zakeriwashed with 1X PBS for 5 min and then stained with 4, 6 -diamidino-2-phenylindole (DAPI) (1 mM) (# ab228549, ABCAM) for 8 min. Cells were washed twice with 1 × PBS, mounted, and embedded in Fluoromount® and observed

(152) High-throughput single nucleus total RNA sequencing of formalin-fixed paraffin-embedded tissues by snRandom-seqNature CommunicationsMay 12, 2023Ziye Xu, Tianyu Zhang, Hongyu Chen, Yuyi Zhu, Yuexiao Lv, Shunji Zhang, Jiaye Chen, Haide Chen, Lili Yang, Weiqin Jiang, et al.aliquot of nuclei was stained with DAPI (4′,6-diamidino-2-phenylindole) staining solution (Abcam, Cat # ab228549), loaded on a hemocytometer and observed under an inverted fluorescence microscope (Nikon Eclipse, Ts2-FL).

(153) Cyp4a12-mediated retinol metabolism in stellate cells is the antihepatic fibrosis mechanism of the Chinese medicine Fuzheng Huayu recipeChinese MedicineMay 9, 2023Da-bing Ping, Xin Sun, Yuan Peng, Cheng-hai Liuthen stained with Cy3 or FITC-conjugated secondary antibodies. After washing, DAPI (1:2000, Abcam, ab228549, UK) was used to stain the nuclei of the tissue. Images were taken with an Olympus confocal microscope,

(154) The mitochondrial Ca 2+ channel MCU is critical for tumor growth by supporting cell cycle progression and proliferationbioRxivMay 3, 2023Emily Fernández García, Usha Paudel, Michael C. Noji, Caitlyn E. Bowman, Jason R. Pitarresi, Anil K. Rustgi, Kathryn E. Wellen, Zolt Arany, Jillian S. Weissenrieder, J. Kevin FoskettScientific, R37174), according to manufacturer’s instructions. After 15-min incubation, 4 mM DAPI (Abcam, ab228549) was added to the suspension and cells were analyzed by FACS with emission filters 530 and 440 nm for

(155) Advanced cell-based products generated via automated and manual manufacturing platforms under the quality by design principle: Are they equivalent or different?HeliyonMay 3, 2023Duc M. Hoang, Quyen T. Nguyen, Trang T.K. Phan, Anh T.L. Ngo, Phuong T. Pham, Trung Q. Bach, Phuong T.T. Le, Hoa T.P. Bui, Liem Nguyen Thanhwere fixed with 4% paraformaldehyde (PFA) and stained with DAPI staining solution (1:5000, Abcam, #ab228549) after the assay. The ImageXpress® Micro Confocal High-content Imaging System (Molecular Devices) was

(156) Lipid Deposition Profiles Influence Foreign Body ResponsesAdvanced materials (Deerfield Beach, Fla.)May 1, 2023Schreib CC, Jarvis MI, Terlier T, Goell J, Mukherjee S, Doerfert MD, Wilson TA, Beauregard M, Martins KN, Lee J, et al.PBST for 3 washes (5 minutes per wash), the mounted slides were incubated with DAPI (1:1000; Abcam ab228549) in 1x PBST at room temperature for 5 minutes. After final washing steps with 1x PBST for 3 washes (5

(157) Selective inhibition of somatostatin-positive dentate hilar interneurons induces age-related cellular changes and cognitive dysfunctionPNAS NexusMay 1, 2023Lyu J, Nagarajan R, Kambali M, Wang M, Rudolph U.microscope. To verify viral placements and immunofluorescence labeling of mCherry, DAPI stain (Abcam, cat # ab228549) was added to free-floating 30-µm coronal sections using the concentration of 1 µm in the dark. The

(158) Acetaldehyde via CGRP receptor and TRPA1 in Schwann cells mediates ethanol-evoked periorbital mechanical allodynia in mice: relevance for migraineJournal of Biomedical ScienceApril 26, 2023Lorenzo Landini, Daniel Souza Monteiro de Araujo, Martina Chieca, Gaetano De Siena, Elisa Bellantoni, Pierangelo Geppetti, Romina Nassini, Francesco De Loguat RT. Slides were then coverslipped with mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, #ab228549, Abcam). Fluorescence images were obtained using an AxioImager 2 microscope (Carl Zeiss). The fluorescence

(159) Insulin Sensitization by PPARγ and GLUT-4 Overexpression/Translocation Mediates the Antidiabetic Effect of Plantago australis †Pharmaceuticals (Basel)April 3, 2023Samuel Estrada-Soto, Kathia Ornelas-Mendoza, Gabriel Navarrete-Vázquez, Fabiola Chávez-Silva, Julio Cesar Almanza-Pérez, Rafael Villalobos-Molina, Erandi Ortiz-Barragán, Hilda Loza-Rodríguez, Julio César Rivera-Leyva, Angélica Flores-Flores, et al.assembly of PBS/glycerol/DAPI (Inorganic Polyphosphate Stores by 4′,6-diamidino-2-phenyl-indole) (Abcam, ab228549) was used as a staining reagent for DNA detection and nuclear integrity. Cell images were taken with

(160) Label-free macrophage phenotype classification using machine learning methodsScientific reportsMarch 30, 2023Hourani T, Perez-Gonzalez A, Khoshmanesh K, Luwor R, Achuthan AA, Baratchi S, O'Brien-Simpson NM, Al-Hourani A.#PKH67GL) as per the manufacturer instructions, and cell nuclei were stained with 0.5 μg/mL DAPI (Abcam, # ab228549) for 5 min at room temperature. Cells were washed again with PBS and the immunofluorescent pictures

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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