DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(141) Omentum-derived matrix enables the study of metastatic ovarian cancer and stromal cell functions in a physiologically relevant environmentMatrix Biology PlusDecember 1, 2023Lisa J. Neilson, Douglas Cartwright, Maija Risteli, Elina M. Jokinen, Lynn McGarry, Toni Sandvik, Konstantina Nikolatou, Kelly Hodge, Samuel Atkinson, Maria Vias, et al.at 1:500 in 1 % BSA/0.1 % saponin in PBS for 1 h RT. Following a wash in 1 % BSA in PBS, Dapi (Abcam ab228549) at 1:2000 in 1 % BSA in PBS was applied for 20 min at room temperature. After a final wash in 1 % BSA

(142) Influence of metabolic stress and metformin on synaptic protein profile in SH‐SY5Y‐derived neuronsPhysiological ReportsNovember 27, 2023Alex J. T. Yang, Ahmad Mohammad, Michael S. Finch, Evangelia Tsiani, Gaynor Spencer, Aleksandar Necakov, Rebecca E. K. MacPhersonbuffer containing phalloidin actin stain (Abcam; ab176757 (Wang et al., 2022)) and DAPI stain (Abcam; ab228549). Images were captured using a Biotek Cytation5 cell imaging reader. Immunofluorescent (IF) images were

(143) Follicle-stimulating hormone orchestrates glucose-stimulated insulin secretion of pancreatic isletsNature CommunicationsNovember 1, 2023Yi Cheng, Hong Zhu, Jun Ren, Hai-Yan Wu, Jia-En Yu, Lu-Yang Jin, Hai-Yan Pang, Hai-Tao Pan, Si-Si Luo, Jing Yan, et al.fluorescent-conjugated secondary antibodies (1:500; Invitrogen, A21241, A11034). Nuclei were stained with DAPI (Abcam, ab228549). All immunofluorescence images were acquired by laser scanning confocal microscope (Zeiss). RNA interference Small

(144) INO80 function is required for mouse mammary gland development, but mutation alone may be insufficient for breast cancerFrontiers in Cell and Developmental BiologyNovember 1, 2023Nguyen Xuan Thang, Dong Wook Han, Chanhyeok Park, Hyeonji Lee, Hyeonwoo La, Seonho Yoo, Heeji Lee, Sang Jun Uhm, Hyuk Song, Jeong Tae Do, et al.Abcam)] in the dark for 1 h, washed with 1X PBS thrice, and briefly counter-stained with DAPI (1:1000, #ab228549; Abcam). The sections were mounted and imaged using a confocal microscope (LSM800; Carl Zeiss, Oberkochen,

(145) Effects of oxytocin on the hair growth ability of dermal papilla cellsScientific ReportsOctober 20, 2023Tatsuto Kageyama, Jieun Seo, Lei Yan, Junji Fukuda°C and lastly with rhodamine-phalloidin (ab235138, Abcam) and 4′,6-diamidino-2-phenylindole (DAPI; ab228549, Abcam) in PBS for 30 min. A confocal microscope (LSM 700; Carl Zeiss, Germany) was used for fluorescence imaging

(146) Small Molecule Pytren-4QMn Metal Complex Slows down Huntington’s Disease Progression in Male zQ175 Transgenic MiceInternational Journal of Molecular SciencesOctober 13, 2023Marián Merino, Sonia González, Mª Carmen Tronch, Ana Virginia Sánchez-Sánchez, Mª Paz Clares, Antonio García-España, Enrique García-España, José L. Mullorantibody Alexa Fluor® 488 (ab150113; Abcam, Cambridge, UK) diluted 1:500 in blocking solution and DAPI (ab228549; Abcam, Cambridge, UK). Finally, the samples were washed three times with PBS 1× and assembled with

(147) Radiotherapy-Induced Neurocognitive Impairment Is Driven by Heightened Apoptotic Priming in Early Life and Prevented by Blocking BAXCancer researchOctober 13, 2023Singh R, Yu S, Osman M, Inde Z, Fraser C, Cleveland AH, Almanzar N, Lim CB, Joshi GN, Spetz J, et al.incubation at 1:200 dilution (Thermo Fisher Scientific A-11008, RRID:AB_143165) and DAPI staining (Abcam ab228549) was performed the next day, with cover slipping using vector labs antifade mounting media (Vector Laboratories

(148) Palbociclib releases the latent differentiation capacity of neuroblastoma cellsDevelopmental CellOctober 9, 2023Kirsty M. Ferguson, Sarah L. Gillen, Lewis Chaytor, Evon Poon, Daniel Marcos, Roshna Lawrence Gomez, Laura M. Woods, Lidiya Mykhaylechko, Louis Elfari, Barbara Martins Martins da Costa, et al.BioReagents™,BP9706-100 Beta-mercaptoethanol,BDH,441433A Trypsin EDTA 0.25%,ThermoFisher Scientific,25200072 DAPI,Abcam,ab228549 DMSO,Santa Cruz Biotechnology,sc-358801 PowerUp™ SYBR™ Green Master Mix,ThermoFisher Scientific,A25742 Crystal

(149) Autophagy-amplifying nanoparticles evoke immunogenic cell death combined with anti-PD-1/PD-L1 for residual tumors immunotherapy after RFAJournal of nanobiotechnologyOctober 3, 2023Zhang S, Huang Y, Pi S, Chen H, Ye F, Wu C, Li L, Ye Q, Lin Y, Su Z.Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077), Goat anti-rabbit IgG H&L (Alexa Fluor® 555) (ab150078), and DAPI Staining Solution (ab228549) from Abcam. The preparation of STF@ZIF-8/PEG-FA (SZP), BMS@ZIF-8/PEG-FA(BZP), and STF-BMS@ZIF-8/PEG-FA

(150) Structural basis of microtubule depolymerization by the kinesin-like activity of HIV-1 RevStructureOctober 1, 2023Elif Eren, Norman R. Watts, Davide Randazzo, Ira Palmer, Dan L. Sackett, Paul T. Wingfield11873580001 Nocodazole,Sigma-Aldrich,Cat# M1404-2MG Taxol (paclitaxel),Invitrogen,Cat# P3456 DAPI staining solution,Abcam,Cat# ab228549 Lipofectamine 3000,Invitrogen,Cat# L3000015 Opti-MEM,Gibco,Cat# 31985070 Subtilisin,Hampton Research

(151) Loss of Grin2a causes a transient delay in the electrophysiological maturation of hippocampal parvalbumin interneuronsCommunications BiologySeptember 19, 2023Chad R. Camp, Anna Vlachos, Chiara Klöckner, Ilona Krey, Tue G. Banke, Nima Shariatzadeh, Sarah M. Ruggiero, Peter Galer, Kristen L. Park, Adam Caccavano, et al.temperature. Slices were washed 3 times in PBS for 10 min then incubated in DAPI counterstain (Abcam, ab228549; 2 µM) for 30 min before being washed 3 more times in PBS for 10 min. Slices were mounted on Superfrost

(152) Microglial SIRT1 activation attenuates synapse loss in retinal inner plexiform layer via mTORC1 inhibitionJournal of neuroinflammationSeptember 5, 2023Yao K, Mou Q, Lou X, Ye M, Zhao B, Hu Y, Luo J, Zhang H, Li X, Zhao Y.anti-Iba1 antibody (ab5076 and ab178846; Abcam; 1:200); anti-CD68 antibody (ab955; Abcam; 1:50); DAPI (ab228549; Abcam; 1:1000); anti-TSC2 antibody (#4308; cell signaling technology; 1:800); anti-HA-Tag antibody

(153) Curcumin-activated Olfactory Ensheathing Cells Improve Functional Recovery After Spinal Cord Injury by Modulating Microglia Polarization Through APOE/TREM2/NF-κB Signaling PathwayJournal of Neuroimmune PharmacologySeptember 2, 2023Chao Jiang, Zhe Chen, Xiaohui Wang, Yongyuan Zhang, Xinyu Guo, Hong Fan, Dageng Huang, Yuqing He, Xiangwen Tang, Yixiang Ai, et al.1:200, Abcam), pNF-κB (3033, 1:200, Cell Signaling Technology), APOE (ab183597, 1:200, Abcam) and DAPI (ab228549, 1:1000, Abcam). After incubation with the corresponding secondary antibodies, coverslips were washed

(154) Self-oxygenation of engineered living tissues orchestrates osteogenic commitment of mesenchymal stem cellsBiomaterialsSeptember 1, 2023Shabir Hassan, Ting Wang, Kun Shi, Yike Huang, Maria Elizabeth Urbina Lopez, Kaifeng Gan, Mo Chen, Niels Willemen, Haroon Kalam, Eder Luna-Ceron, et al.(Catalog No.: ab113851), primary antibodies for F-actin (Catalog No.: ab205) and DAPI (Catalog No.: ab228549) were purchased from Abcam and used as per the manufacturer’s protocol. Recombinant human fibroblast

(155) Long-term survival of LGR5 expressing supporting cells after severe ototoxic trauma in the adult mouse cochleaFrontiers in Cellular NeuroscienceAugust 24, 2023Natalia Smith-Cortinez, Ferry G. J. Hendriksen, Dyan Ramekers, Robert J. Stokroos, Huib Versnel, Louise V. StraatmanInvitrogen, A-21207), donkey-anti Goat-Alexa 488 (1/500, Abcam, AB150129), and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Lastly, specimens were washed in PBS and mounted in Vectashield Antifade

(156) Repetitive Transcranial Magnetic Stimulation Reduces Depressive-like Behaviors, Modifies Dendritic Plasticity, and Generates Global Epigenetic Changes in the Frontal Cortex and Hippocampus in a Rodent Model of Chronic StressCellsAugust 14, 2023Meneses-San Juan D, Lamas M, Ramírez-Rodríguez GB.anti-5-methylcytosine (1:500, Cat: ab214727, Abcam). 4′,6-diamidino-2-phenylindole (DAPI), (1:1000, Cat: ab228549, Abcam) was used to stain the nuclei of the hippocampal dentate gyrus cells. Images were acquired using

(157) Identification of a novel enhancer essential for Satb1 expression in T(H)2 cells and activated ILC2sLife science allianceAugust 1, 2023Nomura A, Kobayashi T, Seo W, Ohno-Oishi M, Kakugawa K, Muroi S, Yoshida H, Endo TA, Moro K, Taniuchi I.kept at −20°C until analysis. On the day of the analysis, thymocytes were stained with 1/100 of DAPI (ab228549; Abcam) at room temperature, for 30 min, and washed three times in PBS. Cover slips were mounted on

(158) Integrated single‐cell and spatial transcriptomic profiling reveals higher intratumour heterogeneity and epithelial–fibroblast interactions in recurrent bladder cancerClinical and Translational MedicineJuly 24, 2023Zhen‐Duo Shi, Zhuo Sun, Zuo‐Bin Zhu, Xing Liu, Jun‐Zhi Chen, Lin Hao, Jie‐Fei Zhu, Kun Pang, Di Wu, Yang Dong, et al.ab75813), CCL22 anti‐human Ab (Abcam, ab23772) and 4′,6‐diamidino‐2‐phenylindole staining solution (Abcam, ab228549). Following the manufacturer's instructions (Akoya, Opal Polaris 7 Color Automation IHC Detection Kit),

(159) Rescue of a Live-Attenuated Porcine Epidemic Diarrhea Virus HSGP Strain Using a Virulent Strain and a Partially Attenuated StrainVirusesJuly 21, 2023Sok Song, Gyu-Nam Park, Jihye Shin, Ki-Sun Kim, Byung-Hyun An, SeEun Choe, Song-Yi Kim, Bang-Hun Hyun, Dong-Jun AnAfter washing, cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Abcam; catalog no. ab228549) at room temperature, followed by observation under a fluorescence microscope (200×). 2.6. Virus Growth

(160) Single‐cell landscape analysis reveals systematic senescence in mammalian Down syndromeClinical and Translational MedicineJuly 17, 2023Yao Chen, Yanyu Xiao, Yanye Zhang, Renying Wang, Feixia Wang, Huajing Gao, Yifeng Liu, Runju Zhang, Huiyu Sun, Ziming Zhou, et al.times with 1% BSA in PBS, the slides were incubated with secondary antibodies (Thermo Fisher) and DAPI (ab228549; Abcam) for 1 h at room temperature. TUNEL staining was performed using a one‐step TUNEL Apoptosis Detection

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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