DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(61) Safeguarding spermatogenesis from retrotransposon insertions by forming ecDNAbioRxivMay 20, 2025Lauren Tracy, ZZ Zhao Zhangroom temperature. Then samples were washed three more times in PBST, with DAPI (1:1000, Abcam Cat. # ab228549) in the second wash step. Finally, the samples were mounted with Vectashield Mounting Medium. Images

(62) Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D culturesJ Tissue EngMay 7, 2025Mercedes Olvera-Valencia, Verónica Garcia-Castillo, Rosalío Ramos-Payan, Maribel Aguilar-Medina, Samuel Trujano-Camacho, Alejandro López-Saavedra, Laurence A. Marchat, Cesar López-Camarillo, Ronen Sumagin, Eloy Pérez-Yepez, et al.(Abcam, ab6940) were diluted in OWB and incubated overnight at 4°C with gentle rocking. DAPI (Abcam, ab228549) was used to stain the nuclei. The stained organoids were mounted on coverslips using an imaging spacer

(63) Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to strokeNat CommunMay 6, 2025Laetitia Thieren, Henri S. Zanker, Jeanne Droux, Urvashi Dalvi, Matthias T. Wyss, Rebecca Waag, Pierre-Luc Germain, Lukas M. von Ziegler, Zoe J. Looser, Ladina Hösli, et al.antibodies. For nuclei labeling, sections were incubated for 10 min in Tris-Triton 0.05% with DAPI (1:10,000, AB228549, Abcam). Sections were mounted on SuperFrost Plus slides (J1800AMNZ, Epredia) in Dako Fluorescence Mounting Medium

(64) Systemic IGF-1 administration prevents traumatic brain injury induced gut permeability, dysmorphia, dysbiosis, and the increased number of immature dentate granule cellsActa neuropathologica communicationsMay 3, 2025Venkatasamy L, Iannucci J, Pereverzev A, Hoar J, Huber E, Ifegbo A, Dominy R, El-Hakim Y, Mani KK, Dabney A, et al.in PBS, and cover slipped with mounting media containing the nuclear dye DAPI (Fluoroshield, Abcam, ab228549). Sections were imaged using a fluorescent microscope (Olympus, Bethlehem, PA). Gut sections probed

(65) Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor-on-a-chip platformBioengineering & translational medicineMay 1, 2025Moya-Garcia CR, Munipalle M, Pacis A, Sadeghi N, Tabrizian M, Li-Jessen NYK.UK",ab133075 MMP‐3 Activity Assay Kit,"Abcam, Cambridge, UK",ab118972 DAPI,"Abcam, Cambridge, UK",ab228549 ALEXA647/EGFR,"Abcam, Cambridge, UK",ab192982 ALEXA488/TUBIII,"Abcam, Cambridge, UK",ab195879 ALEXA594/PI3KCA,"Abcam,

(66) An evaluation of distinct adeno-associated virus vector strategies for driving transgene expression in spinal inhibitory neurons of the ratFront NeurosciMay 1, 2025Laura Klinger, Anna S. M. Siegert, Raphael Holzinger, Lidia Trofimova, Sibel Ada, Ruth Drdla-Schuttingwashed in 1x PBS (3 × 10 min), incubated in 1x PBS + 0.2% 40,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549), guarded from light, for 60 min. Again slices were washed with 1x PBS (2 × 5 min) and mounted on glass

(67) Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in miceNat CommunApril 26, 2025Marion Müller, Torben Schubert, Cornelius Welke, Tibor Maske, Thomas Patschkowski, Elfi Donhauser, Jacqueline Heinen-Weiler, Felix-Levin Hormann, Sven Heiles, Tina Johanna Schulz, et al.secondary ab, A-11034, Invitrogen) on 5 µm murine lung slices. Nuclei were stained with DAPI (1:1000, ab228549, Abcam). Images were acquired with a BZ-X 810 microscope (Keyence, Osaka, Japan) and analyzed using

(68) The HDL-transporting scavenger receptor B1 promotes viral infection through endolysosomal acidificationiScienceApril 24, 2025Jill McConnell, Sun-Young Lim, Thomas Cicuto, Ping Liu, Pyae Phyo Kyawe, Manish Kandpal, Evan S. Bradley, Kendi Okuda, Neal Silverman, Jennifer P. Wang, et al.Sciences,Cat#: 15710 Crystal violet,Sigma-Aldrich,Cat#: V5265 "4′,6-diamidino-2-phenylindole",Abcam,Cat#: ab228549 LysoTracker Red DND-99,ThermoFisher Scientific,Cat#: L7528 LysoSensor Green DND-189,ThermoFisher Scientific,Cat#:

(69) GLUT1 inhibition by BAY-876 induces metabolic changes and cell death in human colorectal cancer cellsBMC CancerApril 17, 2025Masato Hayashi, Keishi Nakamura, Shinichi Harada, Mariko Tanaka, Akiko Kobayashi, Hiroto Saito, Toshikatsu Tsuji, Daisuke Yamamoto, Hideki Moriyama, Jun Kinoshita, et al.at room temperature in the dark. Following TBST washes, 4',6-diamidino-2-phenylindole (DAPI, Abcam, ab228549 1:1000 dilution) in TBST was used for nuclear counterstaining for 20 min at room temperature. All sections

(70) Liver quad culture chip as a model for radiation injury researchSci RepApril 11, 2025Yuki Ueda, Satoshi Omiya, Jonathan Pinney, Michelle A. Bylicky, Molykutty J. Aryankalayilantibodies (Abcam) were then introduced in the channels at RT for 2 h, followed by staining with DAPI (AB228549, DAPI) and Phalloidin (Abcam, AB176759). Microscopy was performed with confocal microscopy (Zeiss Axio Imager

(71) Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone RegenerationAdvanced ScienceMarch 24, 2025Yu‐Xuan Ma, Chen Lei, Tao Ye, Qian‐Qian Wan, Kai‐Yan Wang, Yi‐Na Zhu, Ling Li, Xu‐Fang Liu, Long‐Zhang Niu, Franklin R. Tay, et al.Laboratories, Inc., West Grove, PA, USA). Cell nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI; ab228549; Abcam). The stained sections were imaged using confocal laser scanning microscopy (CLSM, FV1000, Olympus).

(72) Selective promotion of sensory innervation-mediated immunoregulation for tissue repairScience advancesMarch 21, 2025Xu K, Wu K, Chen L, Zhao Y, Li H, Lin N, Ye Z, Xu J, Huang D, Huang X.Proteintech, USA), and PAX6 (1:1000, 67529-1-Ig, Proteintech). 4′,6-Diamidino-2-phenylindole (DAPI; ab228549, Abcam) was used to label the cell nucleus. The staining results were imaged by a confocal laser scanning

(73) Blocking copper transporter protein-dependent drug efflux with albumin-encapsulated Pt(IV) for synergistically enhanced chemo-immunotherapyJ NanobiotechnologyMarch 18, 2025Man Fang, Lei Cao, Zhao Zhang, Zhen Yu, Yue Shen, Jieqi Chen, Juan Chen, Yue-Qin Li, Zhaoqian Liu, Yingcai Meng, et al.followed by processing with corresponding second antibodies. Nuclei were counterstained with DAPI (ab228549, Abcam) and then the stained sections were imaged with a confocal laser scanning microscope. Flow cytometric analysis

(74) The phenotypic characterization of mouse floxed Fam20b chondrocytes, a novel articular cartilage-derived cell line with differentiation potentialRegen TherMarch 13, 2025Xiaoyuan Wu, Lei Zhu, Lifeng Xia, Lin Gui, Wei Conggoat-anti-mouse secondary antibody (ab150120, Abcam). For nucleus staining, the cells were incubated with DAPI (ab228549, Abcam). Cell proliferation was assessed by the percentage of BrdU positive nuclei relative to the total

(75) Pumilio differentially binds to mRNA 3′ UTR isoforms to regulate localization of synaptic proteinsEMBO ReportsFebruary 21, 2025Dominika Grzejda, Anton Hess, Andrew Rezansoff, Sakshi Gorey, Judit Carrasco, Carlos Alfonso-Gonzalez, Stylianos Tsagkris, Lena Neuhaus, Mengjin Shi, Hasan Can Ozbulut, et al.PBS. The penultimate wash included a counterstaining with 4′,6-diamidino-2-phenylindole (DAPI) (Abcam #AB228549) for 10 min at RT. The coverslips were mounted using VECTASHIELD antifade mounting medium (Vector Laboratories

(76) In Vitro, In Vivo, and In Silico Investigation of Synbiotic-Mediated Activation of PPAR-α Curtails Nonalcoholic Steatohepatitis (NASH) in Wistar Rats by Inhibiting PNPLA3/SREBP1-c Lead Inflammatory Injury of Hepatic CellsMediators InflammFebruary 19, 2025Dixa Sharma, Dhara Patel, Palash Mandalcells were incubated with a 4-6-diamidino-2-phenylindole (DAPI) stain of 0.6 µg/mL in PBS for 5 min (ab228549, ABcam, USA). The fluorescence of chromatin was observed under a fluorescence microscope (Leica DMRB

(77) 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and FunctionalizationAdvanced Healthcare MaterialsFebruary 16, 2025Meghan A Robinson, Sonia HY Kung, Khaled YM Youssef, Kali M Scheck, Robert H Bell, Funda Sar, Anne M Haegert, M Mahdi Asmae, Changfeng Cheng, Salina V Yeack, et al.at room temperature. Cells were rinsed another 3 times. 4′,6‐diamidino‐2‐phenylindole (DAPI, Abcam, ab228549) was diluted to 2.5 µM in PBS and added to the cells for 15 min in the dark at room temperature, and

(78) Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundleseLifeFebruary 12, 2025Zheng X, Yu S, Zhou Y, Yu K, Gao Y, Chen M, Duan D, Li Y, Cui X, Mou J, et al.(1:400) Chemical compound,Actin-Tracker Green-488,Beyotime,C2201S,IF (1:100) Chemical compound,DAPI,Abcam,ab228549,IF (1:2000) Chemical compound,Antifade mounting medium,Vectorlabs,H-1400-10,N/A Chemical compound,Purified

(79) Engineering of Genetically Encoded Bright Near-Infrared Fluorescent Voltage IndicatorInt J Mol SciFebruary 8, 2025Xian Xiao, Aimei Yang, Hanbin Zhang, Demian Park, Yangdong Wang, Balint Szabo, Edward S. Boyden, Kiryl D. Piatkevichslices were washed with PBS three times and incubated with DAPI (1:2000 dilution in PBS; Abcam, Cat#ab228549-2m, Waltham, MA, USA) and mounted with ProLong™ Gold Antifade (Thermo Fisher, Cat# P36930, St. Bend,

(80) Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury ModelJournal of Visualized ExperimentsFebruary 7, 2025Marta Stetsiv, Matthew Wan, Shagun Prabhu, Rosa Guzzo, Archana SanjayScientific,22-363-172, "Cryofilm Type II C(10), 3.5 cm","Section Lab, Japan",CFS 105, DAPI Staining Solution,Abcam,ab228549, Digital Gram Scale,Toprime/ Amazon,B06X6LW4V9,Can use any brand scale to weigh mice Dissecting Forceps,Fisherbrand/

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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