DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(61) Selective promotion of sensory innervation-mediated immunoregulation for tissue repairScience advancesMarch 21, 2025Xu K, Wu K, Chen L, Zhao Y, Li H, Lin N, Ye Z, Xu J, Huang D, Huang X.Proteintech, USA), and PAX6 (1:1000, 67529-1-Ig, Proteintech). 4′,6-Diamidino-2-phenylindole (DAPI; ab228549, Abcam) was used to label the cell nucleus. The staining results were imaged by a confocal laser scanning

(62) Blocking copper transporter protein-dependent drug efflux with albumin-encapsulated Pt(IV) for synergistically enhanced chemo-immunotherapyJ NanobiotechnologyMarch 18, 2025Man Fang, Lei Cao, Zhao Zhang, Zhen Yu, Yue Shen, Jieqi Chen, Juan Chen, Yue-Qin Li, Zhaoqian Liu, Yingcai Meng, et al.followed by processing with corresponding second antibodies. Nuclei were counterstained with DAPI (ab228549, Abcam) and then the stained sections were imaged with a confocal laser scanning microscope. Flow cytometric analysis

(63) The phenotypic characterization of mouse floxed Fam20b chondrocytes, a novel articular cartilage-derived cell line with differentiation potentialRegen TherMarch 13, 2025Xiaoyuan Wu, Lei Zhu, Lifeng Xia, Lin Gui, Wei Conggoat-anti-mouse secondary antibody (ab150120, Abcam). For nucleus staining, the cells were incubated with DAPI (ab228549, Abcam). Cell proliferation was assessed by the percentage of BrdU positive nuclei relative to the total

(64) Pumilio differentially binds to mRNA 3′ UTR isoforms to regulate localization of synaptic proteinsEMBO ReportsFebruary 21, 2025Dominika Grzejda, Anton Hess, Andrew Rezansoff, Sakshi Gorey, Judit Carrasco, Carlos Alfonso-Gonzalez, Stylianos Tsagkris, Lena Neuhaus, Mengjin Shi, Hasan Can Ozbulut, et al.PBS. The penultimate wash included a counterstaining with 4′,6-diamidino-2-phenylindole (DAPI) (Abcam #AB228549) for 10 min at RT. The coverslips were mounted using VECTASHIELD antifade mounting medium (Vector Laboratories

(65) In Vitro, In Vivo, and In Silico Investigation of Synbiotic-Mediated Activation of PPAR-α Curtails Nonalcoholic Steatohepatitis (NASH) in Wistar Rats by Inhibiting PNPLA3/SREBP1-c Lead Inflammatory Injury of Hepatic CellsMediators InflammFebruary 19, 2025Dixa Sharma, Dhara Patel, Palash Mandalcells were incubated with a 4-6-diamidino-2-phenylindole (DAPI) stain of 0.6 µg/mL in PBS for 5 min (ab228549, ABcam, USA). The fluorescence of chromatin was observed under a fluorescence microscope (Leica DMRB

(66) 3D Bioprinted Coaxial Testis Model Using Human Induced Pluripotent Stem Cells:A Step Toward Bicompartmental Cytoarchitecture and FunctionalizationAdvanced Healthcare MaterialsFebruary 16, 2025Meghan A Robinson, Sonia HY Kung, Khaled YM Youssef, Kali M Scheck, Robert H Bell, Funda Sar, Anne M Haegert, M Mahdi Asmae, Changfeng Cheng, Salina V Yeack, et al.at room temperature. Cells were rinsed another 3 times. 4′,6‐diamidino‐2‐phenylindole (DAPI, Abcam, ab228549) was diluted to 2.5 µM in PBS and added to the cells for 15 min in the dark at room temperature, and

(67) Interleukin-1 prevents SARS-CoV-2-induced membrane fusion to restrict viral transmission via induction of actin bundleseLifeFebruary 12, 2025Zheng X, Yu S, Zhou Y, Yu K, Gao Y, Chen M, Duan D, Li Y, Cui X, Mou J, et al.(1:400) Chemical compound,Actin-Tracker Green-488,Beyotime,C2201S,IF (1:100) Chemical compound,DAPI,Abcam,ab228549,IF (1:2000) Chemical compound,Antifade mounting medium,Vectorlabs,H-1400-10,N/A Chemical compound,Purified

(68) Engineering of Genetically Encoded Bright Near-Infrared Fluorescent Voltage IndicatorInt J Mol SciFebruary 8, 2025Xian Xiao, Aimei Yang, Hanbin Zhang, Demian Park, Yangdong Wang, Balint Szabo, Edward S. Boyden, Kiryl D. Piatkevichslices were washed with PBS three times and incubated with DAPI (1:2000 dilution in PBS; Abcam, Cat#ab228549-2m, Waltham, MA, USA) and mounted with ProLong™ Gold Antifade (Thermo Fisher, Cat# P36930, St. Bend,

(69) Improved Methodology for Studying Postnatal Osteogenesis via Intramembranous Ossification in a Murine Bone Marrow Injury ModelJournal of Visualized ExperimentsFebruary 7, 2025Marta Stetsiv, Matthew Wan, Shagun Prabhu, Rosa Guzzo, Archana SanjayScientific,22-363-172, "Cryofilm Type II C(10), 3.5 cm","Section Lab, Japan",CFS 105, DAPI Staining Solution,Abcam,ab228549, Digital Gram Scale,Toprime/ Amazon,B06X6LW4V9,Can use any brand scale to weigh mice Dissecting Forceps,Fisherbrand/

(70) Pharmacological Inhibition of N-Acylethanolamine Acid Amidase (NAAA) Mitigates Intestinal Fibrosis Through Modulation of Macrophage ActivityJournal of Crohn's and ColitisFebruary 4, 2025Maria Francesca Nanì, Ester Pagano, Paola De Cicco, Giuseppe Lucariello, Fabio Cattaneo, Francesca Paola Tropeano, Donatella Cicia, Rebecca Amico, Federica Raucci, Giuseppe Ercolano, et al.microscope slides using Mounting Medium (Cat. #AB104135, Abcam) and then counterstained with DAPI (Cat. #AB228549, Abcam) for nuclear staining. The images were acquired at room temperature and detected under 40× magnification

(71) Definitive Evidence for the Identification and Function of Renin-Expressing Cholinergic Neurons in the Nucleus AmbiguusHypertensionFebruary 1, 2025Éva M. Fekete, Javier Gomez, Mina Ghobrial, Kathren Kaminski, Patricia C. Muskus, Carie R. Boychuk, Ana Hantke Hantke Guixa, Ibrahim Vazirabad, Michelle Xie, Azeez Ganiyu, et al.,ACD Bio,310043 Chemicals,, Propranolol,Millipore-Sigma,P0884-5G Methyl-Atropine,Millipore-Sigma,M1300000 Phenylephrine hydrochloride,Millipore-Sigma,P6126-5G DAPI,Abcam,Ab228549 ,, Key resource table

(72) 22 and 50 kHz rat ultrasonic vocalization playback reveals sex differences in behavior and cFos in brain regions associated with affective processingBehavioural Brain ResearchFebruary 1, 2025Sydney M. Bonauto, Kaya A. Patel, Jennifer A. Honeycutt(ab6564, Abcam)). Tissue was washed 3 × 5 min in PBS, then incubated for 30 minutes in 1:1000 DAPI (ab228549, Abcam) in PBS. Finally, brain sections were washed 3 × 5 min, mounted in glass slides, and coverslipped

(73) Unraveling the Microenvironment and the Pathogenic Axis of HIF‐1α–Visfatin–Fibrosis in Autoimmune Pancreatitis Using a Single‐Cell AtlasAdvanced ScienceJanuary 31, 2025Deyu Zhang, Congjia Ma, Zhen Wang, Yanfang Liu, Zaoqu Liu, Wanshun Li, Yue Liu, Chang Wu, Liqi Sun, Fei Jiang, et al.were incubated with multiple antibodies and counterstained with 4′,6‐diamidino‐2‐phenylindole (DAPI) (ab228549, Abcam, Cambridge, UK). Finally, the slides were observed under a fluorescence microscope. Cell Culture Human

(74) Stroma-derived Dickkopf-1 contributes to the suppression of NK cell cytotoxicity in breast cancerNat CommunJanuary 30, 2025Seunghyun Lee, Biancamaria Ricci, Jennifer Tran, Emily Eul, Jiayu Ye, Qihao Ren, David Clever, Julia Wang, Pamela Wong, Michael S. Haas, et al.(21112-1-AP)) followed by anti-rabbit-Alexa 488 secondary antibody (Abcam, ab150077, 1:1000) and DAPI (Abcam, ab228549) for an hour and mounted with anti-fade fluorescence mounting medium (Abcam). PyMT-mCherry tumor cells

(75) Establishment of a chemoresistant laryngeal cancer cell model to study chemoresistance and chemosensitization responses via transcriptomic analysis and a tumor‐on‐a‐chip platformBioengineering & Translational MedicineJanuary 22, 2025Christian R. Moya‐Garcia, Meghana Munipalle, Alain Pacis, Nader Sadeghi, Maryam Tabrizian, Nicole Y. K. Li‐JessenUK",ab133075 MMP‐3 Activity Assay Kit,"Abcam, Cambridge, UK",ab118972 DAPI,"Abcam, Cambridge, UK",ab228549 ALEXA647/EGFR,"Abcam, Cambridge, UK",ab192982 ALEXA488/TUBIII,"Abcam, Cambridge, UK",ab195879 ALEXA594/PI3KCA,"Abcam,

(76) Intrathecal transplantation of human umbilical cord mesenchymal stem cells enhances spinal cord injury recovery: Role of miR‑124‑3p as a biomarkerExp Ther MedJanuary 22, 2025Yitong Zheng, Yongxin Wang, Wen Liu, Mujite A, Yabin Li, Xiaohu Ma, Mieradili Abulimiti, Nuerailijiang Maimaitiaili, Hu Qinincubation at 37˚C for 30 min. DAPI staining (dilution, 1:1,000; 10 min at room temperature; Abcam; cat. no. ab228549) was used to label cell nuclei. Following washing with PBS, the sections were observed and imaged under

(77) Rapid and scalable personalized ASO screening in patient-derived organoidsNatureJanuary 22, 2025John C. Means, Anabel L. Martinez-Bengochea, Daniel A. Louiselle, Jacqelyn M. Nemechek, John M. Perry, Emily G. Farrow, Tomi Pastinen, Scott T. Youngerantibody incubation, cardiac organoids were washed 5 × 3 min in 1X PBS. After secondary incubation, DAPI (AB228549, Abcam) was added at 1:1,000 for 15 min at room temperature, followed by three washes with 1X PBS. Antibody

(78) Enhancing Cytoplasmic Expression of Exogenous mRNA Through Dynamic Mechanical StimulationAdvanced Healthcare MaterialsJanuary 1, 2025Jiawen Chen, Aneri Patel, Mohammad Mir, Maria R. Hudock, Meghan R. Pinezich, Brandon Guenthart, Matthew Bacchetta, Gordana Vunjak‐Novakovic, Jinho Kimsolution (Cat. No. 30–2300) were purchased from ATCC. 4,6-diamidino-2-phenylindole (DAPI) (Cat. No. ab228549) was purchased from Abcam. EGFP mRNA-lipid nanoparticle (LNP) (Cat. No. PM-LNP-0021) was purchased from

(79) Small spheroids for head and neck cartilage tissue engineeringScientific ReportsDecember 30, 2024Sven Reutter, Johann Kern, Yvonne Jakob, Nicole Rotter, David Gvaramiamanufacturer’s instructions. Total cell nuclei were counterstained using a DAPI staining solution (ab228549, Abcam) (Figs. 1, 2, 3 and 4). Image analysis To analyze the viability, mitochondrial ATP staining,

(80) CD38 Coordinates with NF-κB to Promote Cochlear Inflammation in Noise-Induced Hearing Loss: the Protective Effect of ApigeninMolecular NeurobiologyDecember 27, 2024Da Liu, Na Sai, Ying Zhou, Ning Yu, Qing-qing Jiang, Wei Sun, Wei-ju Han, Weiwei Guo(Proteintech, 60,006–1-lg). Rinse thoroughly and seal the tablet with DAPI-containing glycerin (Abcam, ab228549). The specimens were observed by excitation light at 405 nm (blue), 488 nm (green), and 555 nm (red)

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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