DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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Website: http://www.abcam.com

(41) TNF‑α induces premature senescence in tendon stem cells via the NF‑κB and p53/p21/cyclin E/CDK2 signaling pathwaysInt J Mol MedJuly 10, 2025Hua Guo, Haixia Cao, Qian Lu, Zhifeng Gu, Guijuan FengAbcam] at 1:250 were added for 2 h at room temperature. Nuclei were counterstained with DAPI (cat. no. ab228549, Abcam) at 1:500 at room temperature for 30 min and cells were examined using a fluorescence microscope

(42) Paradoxical regulation of IGF2 in promoting lipid metabolism in adipose tissuesCommun BiolJuly 9, 2025Caiping Zhou, Weiwei Gui, Weifen Zhu, Hanxin Zhao, Dingting Wu, Fang Wu, Gangliang Wang, Xihua Linfluorescent (FITC-conjugated IgG) secondary antibodies. Nuclear staining was executed using DAPI (Abcam, ab228549). Three sections at least 160 μm apart were subjected to insulin immunohistochemistry staining and scanned

(43) Deciphering the tumor immune microenvironment: single-cell and spatial transcriptomic insights into cervical cancer fibroblastsJ Exp Clin Cancer ResJuly 5, 2025Zhiheng Lin, Youwei Zhou, Zhenran Liu, Wenyang Nie, Hengjie Cao, Shengnan Li, Xuanling Li, Lijun Zhu, Guangyao Lin, Yanyu Ding, et al.[ab150116, 1:500], Abcam) were applied for 1 h at RT in the dark. Nuclei were counterstained with DAPI (ab228549, 1:1000, Abcam) for 10 min before mounting with Fluoromount-G (SouthernBiotech, USA). Imaging was performed

(44) Intermediate cell states in spermatogonial stem cell differentiation: enhancing neurogenesis and angiogenesis in spinal cord injuryEur J Med ResJuly 2, 2025Xinyu Guo, Hao Yang, Haihong Zhanganti-mouse IgG antibody, 1:1000) incubation was performed at room temperature for 1 h. DAPI (1:1000; Abcam, ab228549) was added before microscopic observation. Fluorescent signals were documented using a dedicated fluorescence imaging

(45) Mucosal Mixed Lymphocyte Reaction Assay Using Intestinal Lymphocytes as a Biomarker for Intestinal Transplant Tolerance DevelopmentTransplantationJuly 1, 2025M. Esad Gunes, Satyajit Patwardhan, Julie Hong, Elin Manell, Philip Jordache, Ishit Chauhan, Ahmed Almesallmy, Jianing Fu, Megan Sykes, Joshua WeinerUSA, Cat: 124260250). 5μL of DAPI (10mM) diluted 1:10000 with wash buffer (Abcam, Waltham, MA, Cat: ab228549) was added immediately before acquisition to distinguish dead cells. Flow Cytometry Acquisition The

(46) Harnessing cell-encapsulated hydrogels to study astrocyte mechanoresponse in 4DbioRxivJune 27, 2025Ana N. Strat, Suhani Patel, Minh-Tri Ho Thanh, Alexander Kirschner, Souvik Ghosh, Michael P. Geiss, Mariano Viapiano, Yutao Liu, Alison E. Patteson, Samuel Herberg, et al.antibody to rabbit IgG, 1:500, Abcam, Ab150077) for 1 h; nuclei were counterstained with DAPI (Abcam, Ab228549). Sections were mounted with ProLong™ Gold Antifade (Thermo Fisher Scientific, P36930, Waltham, MA,

(47) Utidelone Suppresses PDAC Growth and Enhances Gemcitabine Therapy by Inducing Immunogenic Cell DeathiScienceJune 20, 2025Jingyi Zhou, Kangnan Zhang, Chuan Liu, Jiang Long, Haitao Gu, Hanguang Dong, Chuntao Wu, Ling Xu, Fan Wang, Qi LiCalf Serum,Sigma-Aldrich,F4135 ʟ-Glutamine–Penicillin–Streptomycin,Sigma-Aldrich,G6784 cell counting kit-8 (CCK-8) reagent,DOJINDO,CK04 DAPI,Abcam,ab228549 phorbol myristate acetate (50 ng/mL),Sigma-Aldrich,P8139 monensin (10 μg/mL),Sigma-Aldrich,475897 ionomycin

(48) Molecular signatures of regional vulnerability to tauopathy in excitatory cortical neuronsActa NeuropathologicaJune 7, 2025Diede W. M. Broekaart, Abhijeet Sharma, Aarthi Ramakrishnan, Anjalika Chongtham, Dorothee M. Günther, Saraswathi Subramaniyan, Minghui Wang, Vishwendra Patel, Bin Zhang, Lea T. Grinberg, et al.washed with PBS and incubated with 4’−6-diamidino-2-phenylindole (DAPI) staining solution (1:10.000, ab228549, Abcam) for 5 minutes before being coverslipped with Prolong Diamond Antifade Mountant (P36970, Invitrogen,

(49) Polystyrene nanoplastics trigger pyroptosis in dopaminergic neurons through TSC2/TFEB-mediated disruption of autophagosome-lysosome fusion in Parkinson’s diseaseJ Transl MedJune 5, 2025Xiaomei Liang, Yaqi Zeng, Piao Zhang, Baoyu Zhu, Jiezhu Feng, Tongtong Deng, Zhongling Fu, Chengshuai Liu, Chengyu Chen, Yuhu Zhangand Alexa Fluor 594-conjugated (A-11037, Thermo Fisher Scientific, USA) secondary antibodies. DAPI (ab228549, Abcam, UK) was then applied to stain nuclei. Fluorescence signals were visualized using a confocal

(50) KSR1 Mediates Small Cell Lung Carcinoma Tumor Initiation and Cisplatin ResistanceMolecular Cancer ResearchJune 3, 2025Deepan Chatterjee, Robert A. Svoboda, Dianna H. Huisman, Benjamin J. Drapkin, Heidi M. Vieira, Chaitra Rao, James W. Askew, Kurt W. Fisher, Robert E. LewisAssay For colony formation assays, SCLC cells were dissociated by gentle pipetting. Cells were DAPI (Abcam #ab228549) stained for viability determination and live GFP+/RFP+ cells were single cell sorted as one cell per

(51) Safeguarding spermatogenesis from retrotransposon insertions by forming ecDNAbioRxivMay 20, 2025Lauren Tracy, ZZ Zhao Zhangroom temperature. Then samples were washed three more times in PBST, with DAPI (1:1000, Abcam Cat. # ab228549) in the second wash step. Finally, the samples were mounted with Vectashield Mounting Medium. Images

(52) Development of a reliable method for human triple-negative breast cancer organotypic culture: Improving imaging and genomic studies in 3D culturesJ Tissue EngMay 7, 2025Mercedes Olvera-Valencia, Verónica Garcia-Castillo, Rosalío Ramos-Payan, Maribel Aguilar-Medina, Samuel Trujano-Camacho, Alejandro López-Saavedra, Laurence A. Marchat, Cesar López-Camarillo, Ronen Sumagin, Eloy Pérez-Yepez, et al.(Abcam, ab6940) were diluted in OWB and incubated overnight at 4°C with gentle rocking. DAPI (Abcam, ab228549) was used to stain the nuclei. The stained organoids were mounted on coverslips using an imaging spacer

(53) Astrocytic GLUT1 deletion in adult mice enhances glucose metabolism and resilience to strokeNat CommunMay 6, 2025Laetitia Thieren, Henri S. Zanker, Jeanne Droux, Urvashi Dalvi, Matthias T. Wyss, Rebecca Waag, Pierre-Luc Germain, Lukas M. von Ziegler, Zoe J. Looser, Ladina Hösli, et al.antibodies. For nuclei labeling, sections were incubated for 10 min in Tris-Triton 0.05% with DAPI (1:10,000, AB228549, Abcam). Sections were mounted on SuperFrost Plus slides (J1800AMNZ, Epredia) in Dako Fluorescence Mounting Medium

(54) Systemic IGF-1 administration prevents traumatic brain injury induced gut permeability, dysmorphia, dysbiosis, and the increased number of immature dentate granule cellsActa neuropathologica communicationsMay 3, 2025Venkatasamy L, Iannucci J, Pereverzev A, Hoar J, Huber E, Ifegbo A, Dominy R, El-Hakim Y, Mani KK, Dabney A, et al.in PBS, and cover slipped with mounting media containing the nuclear dye DAPI (Fluoroshield, Abcam, ab228549). Sections were imaged using a fluorescent microscope (Olympus, Bethlehem, PA). Gut sections probed

(55) An evaluation of distinct adeno-associated virus vector strategies for driving transgene expression in spinal inhibitory neurons of the ratFront NeurosciMay 1, 2025Laura Klinger, Anna S. M. Siegert, Raphael Holzinger, Lidia Trofimova, Sibel Ada, Ruth Drdla-Schuttingwashed in 1x PBS (3 × 10 min), incubated in 1x PBS + 0.2% 40,6-diamidino-2-phenylindole (DAPI, Abcam, ab228549), guarded from light, for 60 min. Again slices were washed with 1x PBS (2 × 5 min) and mounted on glass

(56) Nitro-oleic acid enhances mitochondrial metabolism and ameliorates heart failure with preserved ejection fraction in miceNat CommunApril 26, 2025Marion Müller, Torben Schubert, Cornelius Welke, Tibor Maske, Thomas Patschkowski, Elfi Donhauser, Jacqueline Heinen-Weiler, Felix-Levin Hormann, Sven Heiles, Tina Johanna Schulz, et al.secondary ab, A-11034, Invitrogen) on 5 µm murine lung slices. Nuclei were stained with DAPI (1:1000, ab228549, Abcam). Images were acquired with a BZ-X 810 microscope (Keyence, Osaka, Japan) and analyzed using

(57) The HDL-transporting scavenger receptor B1 promotes viral infection through endolysosomal acidificationiScienceApril 24, 2025Jill McConnell, Sun-Young Lim, Thomas Cicuto, Ping Liu, Pyae Phyo Kyawe, Manish Kandpal, Evan S. Bradley, Kendi Okuda, Neal Silverman, Jennifer P. Wang, et al.Sciences,Cat#: 15710 Crystal violet,Sigma-Aldrich,Cat#: V5265 "4′,6-diamidino-2-phenylindole",Abcam,Cat#: ab228549 LysoTracker Red DND-99,ThermoFisher Scientific,Cat#: L7528 LysoSensor Green DND-189,ThermoFisher Scientific,Cat#:

(58) GLUT1 inhibition by BAY-876 induces metabolic changes and cell death in human colorectal cancer cellsBMC CancerApril 17, 2025Masato Hayashi, Keishi Nakamura, Shinichi Harada, Mariko Tanaka, Akiko Kobayashi, Hiroto Saito, Toshikatsu Tsuji, Daisuke Yamamoto, Hideki Moriyama, Jun Kinoshita, et al.at room temperature in the dark. Following TBST washes, 4',6-diamidino-2-phenylindole (DAPI, Abcam, ab228549 1:1000 dilution) in TBST was used for nuclear counterstaining for 20 min at room temperature. All sections

(59) Liver quad culture chip as a model for radiation injury researchSci RepApril 11, 2025Yuki Ueda, Satoshi Omiya, Jonathan Pinney, Michelle A. Bylicky, Molykutty J. Aryankalayilantibodies (Abcam) were then introduced in the channels at RT for 2 h, followed by staining with DAPI (AB228549, DAPI) and Phalloidin (Abcam, AB176759). Microscopy was performed with confocal microscopy (Zeiss Axio Imager

(60) Silicon Enhances Functional Mitochondrial Transfer to Improve Neurovascularization in Diabetic Bone RegenerationAdvanced ScienceMarch 24, 2025Yu‐Xuan Ma, Chen Lei, Tao Ye, Qian‐Qian Wan, Kai‐Yan Wang, Yi‐Na Zhu, Ling Li, Xu‐Fang Liu, Long‐Zhang Niu, Franklin R. Tay, et al.Laboratories, Inc., West Grove, PA, USA). Cell nuclei were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI; ab228549; Abcam). The stained sections were imaged using confocal laser scanning microscopy (CLSM, FV1000, Olympus).

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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