DAPI Staining Solution ab228549 from Abcam

Product Specs
ItemDAPI Staining Solution
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab228549
Quantity2 mL
Sample TypeAdherent cells, Suspension cells
TypeStain

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Abcam

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(21) Heat-shock pathway activation by TRC051384 protects spiral ganglion neurons from noise-induced hearing lossbioRxivDecember 11, 2025Jintao Yu, Miguel A. Ramirez, Yi-Zhi Wang, Seby Edassery, Maxwell Shramuk, Mary Ann Cheatham, Mark A. Rutherford, Leah J Welty, Jeffrey N Savasincubation, samples were washed three times in PBS (15 min each) and counterstained with DAPI solution (Abcam ab228549) for 5 min. Tissues were mounted using ProLong Gold Antifade Mountant (Thermo Scientific, Cat# P10144).

(22) MEF2C controls segment-specific gene regulatory networks that direct heart tube morphogenesisGenes & DevelopmentDecember 1, 2025Jonathon M. Muncie-Vasic, Tanvi Sinha, Alexander P. Clark, Emily F. Brower, Jeffrey J. Saucerman, Brian L. Black, Benoit G. Bruneaumin in the same blocking buffer at 37°C with gentle rotation. Secondary antibodies and DAPI (Abcam, ab228549, used at 1 μg/mL) were added in a blocking buffer consisting of PBS (Life Technologies, 14190250), 5%

(23) Immunocompetent Murine Models Recapitulate the Heterogeneous Tumor-Immune Microenvironment of Human LiposarcomaClinical Cancer ResearchDecember 1, 2025Amanda M. Shafer, Emma Kenna, Lexi-Ann F. Golden, Ahmed M. Elhossiny, Kyle D. Perry, Jodi Wilkowski, Wei Yan, Brynn Kaczkofsky, Jake McGue, Scott C. Bresler, et al.Roche). See Supplementary Table S1 for antibody details. DAPI (4′,6-diamidino-2-phenylindole; Abcam, AB228549) was used to stain nuclei. mT/mG indicator mice samples were stained for GFP and tdTomato using the

(24) Loss of the RNA Binding Protein HuR in Early Murine Limb Mesenchyme Does Not Affect Development but Leads to Impaired Bone Homeostasis in AdulthoodThe FASEB JournalNovember 30, 2025Shijian Fu, Kirsty A. Johnson, Phaedra Winstanley‐Zarach, Ufuk Ersoy, Elena Adlmanninger, Benjamin T. McDermott, Craig Keenan, Aphrodite Vasilaki, Ioannis Kanakis, Peter I. Milner, et al.hairpin amplifier overnight at room temperature. The embryos' cell nuclei were then stained with DAPI (ab228549, Abcam) before imaging. HCR probe sets were designed following previously reported protocols [18, 19].

(25) Altered Cell Surface N -Glycosylation Implicates Hypersialylation in Breast Cancer Brain MetastasisJournal of Proteome ResearchNovember 7, 2025Judith Nwaiwu, Wenjing Peng, Akhila Reddy, Xue Dong, Parisa Ahmadi, Jingfu Zhao, Yifan Huang, Waziha Purba, Peilin Jiang, Oluwatosin Daramola, et al.Paraformaldehyde was purchased from Fisher Scientific (Fair Lawn, New Jersey, USA). DAPI solution (Cat. no: AB228549) was acquired from Abcam (Waltham, MA, USA). Concanavalin A Alexa Fluor ™ 647 conjugate (Cat. no: C21421),

(26) Mitofusin 1 in mitochondrial quality control and anti-inflammatory responses in nucleus pulposus cells during disc degenerationScientific ReportsNovember 4, 2025Jae Won Shin, Young Mi Kang, Hak Sun Kim, Seong Hwan Moon, Kyung Soo Suk, Si Young Park, Byung Ho Lee, Ji Won Kwondilution (Invitrogen; Thermo Fisher Scientific) and 10 μM 4′,6-diamidino-2-phenylindole (DAPI; Abcam, ab228549, Milton, UK) for nuclear staining at room temperature (20–22 °C) for 1 h. The samples were examined

(27) Sex differences in response to kanamycin-induced ototoxicity in C57BL/6 J miceScientific ReportsOctober 31, 2025Natalia Smith-Cortinez, Louise V. Straatman, Ferry G. J. Hendriksen, Robert J. Stokroos, Huib Versnelantibodies donkey-anti-rabbit-Alexa 594 (1/500, Invitrogen, A-21207) and DAPI solution (1/500, Abcam, AB228549) for 90 min at room temperature. Finally, the samples were washed in PBS and mounted in Vectashield

(28) Breaking the link between morphology and potency for mESCsCell BiosciOctober 23, 2025Yixin Fan, Xiaomin Wang, Ziwei Zhai, Tao Huang, Wei Li, Zechuan Liang, Zhaoyi Ma, Yu Fu, Pengli Li, Junqi Kuang, et al.(BBI) for 15 min at 37 °C. Washed the cells again with PBS, stained with DAPI stanning solution (Abcam, ab228549) for 10 min at room temperature. Images were captured with Confocal Microscope LSM 980 (Zeiss). Cell viability assay

(29) Glutamate decreases oxidative stress and lipid droplet formation in astrocytesJournal of Cell ScienceOctober 1, 2025Luis Fernando Rubio-Atonal, Jinlan Chang, Julie Jacquemyn, Isha Ralhan, Iset Ilarraza, Maria S. Ioannou(Thermo Fisher Scientific, A11008, RRID: AB_143165; 1:750). Cells were washed, stained with DAPI (Abcam, ab228549), washed and mounted in DAKO Medium (Agilent, S302380-2). Microscopy Imaging was performed using a

(30) Elevated Transglutaminase-2 in SOX10-Deficient Melanoma Promotes Tumor Onset and Decreases Intratumoral CD4+ T CellsCancer ResearchOctober 1, 2025Signe Caksa, Timothy J. Purwin, Dan A. Erkes, Kristen M. DeRosa, Erica Kitterman, Samantha M. Barnada, Casey D. Stefanski, Haley P. Wilson, Glenn L. Mersky, McKenna Q. Glasheen, et al.antibody (Invitrogen, #A-11034, 1:1000, RRID: AB_2576217) and with DAPI Staining Solution (Abcam, #ab228549, 1:1000) diluted in Odyssey Blocking Buffer for 30 minutes. Coverslips were then washed three times

(31) Ti3C2Tx MXene Potentiates PSAT1-Mediated Osteogenesis Through miR-665/GSK-3β/β-Catenin Axis to Counteract Inflammation-Induced Bone LossInternational Journal of NanomedicineSeptember 12, 2025Jinlong Zhang, Xinyang Wang, Jingwen Xiao, Hongxiang Hong, Jiajia Chen, Chunshuai Wu, Guanhua Xu, Zhiming Cuihours at room temperature. Nuclei stained with 4’ 6-diamidino-2-phenylindole (DAPI) (1:800, Abcam, ab228549). Representative images were observed by a Leica fluorescence microscope (Germany). Quantitative Real-Time PCR

(32) CDK4/6 inhibitors display a class effect in inducing differentiation of neuroblastoma cellsWellcome Open ResearchSeptember 3, 2025Kirsty M. Ferguson, Fiona M. Y. Abou Grealy, Anna Philpottblocking solution (1 hr, RT) and washed again. Nuclear counterstaining was performed using DAPI (Abcam, ab228549) (1:10000 in PBST, 15 min, RT). Imaging was performed on a Leica DMI 6000B Matrix microscope with Leica

(33) Isotope-encoded spatial biology identifies plaque-age-dependent maturation and synaptic loss in an Alzheimer’s disease mouse modelNature CommunicationsSeptember 1, 2025Jack I. Wood, Maciej Dulewicz, Alicja Szadziewska, Sophia Weiner, Junyue Ge, Katie Stringer, Sneha Desai, Lydia Fenson, Diana Piotrowska, Gunnar Brinkmalm, et al.(Thermo Fisher Scientific, #A-21209)). Nuclei were counterstained with 4ʹ,6-diamidino-2- phenylindole (Abcam, #ab228549) for 5 minutes at RT. For structural amyloid analysis, tissues were stained with two luminescent conjugated

(34) A Decoy Oligodeoxynucleotides Disturbing Forkhead Box O3 Mediated ctnna2 Transcriptional Repression Prevents Postoperative Neurocognitive Disorder in MiceCNS Neurosci TherAugust 26, 2025Zhixin Wu, Dongkun Xie, Jing Zhao, Jianshuai Zhao, Huiqing Liu, Dong Xing, Tingting Gu, Yaru Guo, Dan Wang, Zhihong Lu, et al.room temperature. The slides were washed and cover‐slipped with mounting media containing DAPI (Abcam, ab228549, 1:10000) for observation under a confocal laser scanning microscope (FV10, Olympus, Japan). 2.6.2

(35) Yes-Associated Protein (YAP) Is Associated With Disease Severity and Visual Prognosis in Epiretinal MembraneInvest Ophthalmol Vis SciAugust 18, 2025Hyun Seung Yang, Yong-Ha Jo, Dongwoo Kim, Suho Choi, Byung Gil Moon, Youngseop Lee, Minji Kim, Donghoon Koo, Jeongmin Kimstaining was performed by incubating tissue sections with 4′,6-diamidino-2-phenylindole (DAPI, 1:400, ab228549; Abcam, Cambridge, UK) for nuclear staining, along with primary antibodies against rabbit anti-COL1

(36) NXF1 suppresses progression of endometrial cancer by interacting with the SRSF3 to regulate SP4 splicingiScienceAugust 15, 2025Nan Meng, Lingjun Li, Xueqin Yang, Hui Tang, Jizhong Wang, Songbai Zhang, Zongbao Xia, Jia Yao, Qi Zhang, Changrong Hu, et al.and recombinant proteins" ,, Lipofectamine™ 2000,Invitrogen™,11668019 DAPI Staining Solution,Abcam,ab228549 Lipofectamine™ RNAiMAX,Thermo Fisher Scientific,13778030 Co-IP lysis buffer,Beyotime,P0038 protein A/G plus agarose

(37) GPD1L as a potential biomarker associated with Treg cell infiltration and lipid metabolism in clear cell renal cell carcinomaJ Transl MedAugust 6, 2025Ming Yang, Dejiang Pang, Chuhui Gong, Kangping Song, Hongbo Ma, Yu Yang, Shujin Guo, Liqiong Wangprocedures as previously reported [21, 22]. The antibodies and reagents used in this process, such as DAPI (#ab228549, Abcam, 1:1000), anti-CD4 (Rabbit, #ab133616, Abcam, 1:200), anti-CD25 (Rabbit, #ab9496, Abcam, 1:200),

(38) Ginsenoside Rk1 Enhances Chemosensitivity of Gastric Cancer Through Activating the AMPK/mTOR PathwayDose ResponseAugust 5, 2025Yaqin Huang, Jian Wu, Li Zheng, Gen Huang, Bin Li, Lihong Gan, Ling YaoBrdU (Cat.No. ab220074, Abcam, Cambridge, UK). 4′-6-diamidino-2-phenylindole (DAPI) solution (Cat.No. ab228549, Abcam, Cambridge, UK) was used to stain the nuclei for 5 minutes. Finally, a fluorescence microscope

(39) Transcriptome-wide regulation of folate on neural mRNA m6A methylome via carbon metabolism in Drosophila and mammalsCommun BiolAugust 4, 2025Anrui Liu, Zhengyi Huo, Zihe Xu, Sisi Liu, Yuting Jiao, Rui Li, Yi Lu, Meng Yang, Jia Huang, Chen Huang, et al.Invitrogen) for 1 h. The slides were mounted with nail polish and stained with DAPI Staining Solution (#ab228549, Abcam). A FLUOVIEW FV3000 confocal laser scanning microscope (Olympus, Shinjuku, Japan) was used to

(40) N6-methyladenosine (m6A) dysregulation contributes to network excitability in temporal lobe epilepsyJCI InsightJuly 22, 2025Justine Mathoux, Marc-Michel Wilson, Sujithra Srinivas, Gabrielle Litovskich, Leticia Villalba Benito, Cindy Tran, Jaideep Kesavan, Aileen Harnett, Theresa Auer, Amaya Sanz-Rodriguez, et al.buffer along with secondary antibodies. Coverslips were mounted using mounting medium with DAPI (Abcam, ab228549) and imaged using a Zeiss LSM 800 confocal microscope. For PSD95 and dendritic spine quantification,

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6/17/2020This DAPI Binds Strongly to Nuclei Samantha LanjewarIt stains nuclei well and is easy to use.

DAPI Staining Solution from Abcam

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DAPI Staining Solution from Abcam

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