GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(161) Apelin Receptor (APJ) Expression during Cardiopulmonary Bypass in Children Undergoing Surgical RepairPLoS ONESeptember 3, 2014Susan Walker, Mark H. D. Danton, Alexander D. Lang, Fiona LyallQuantiTect Reverse transcription Kit (Qiagen, 205310) and GoScript reverse transcriptase (Promega, A5003) the RNA was reverse transcribed into cDNA. The products of the PCR reaction were separated on a 2%

(162) Use of surface enhanced blocking (SEB) electrodes for microbial cell lysis in flow-through devicesPloS oneJanuary 1, 2014Abdossamad Talebpour, Robert Maaskant, Aye Aye Khine, Tino AlavieRobust HotStart 2X PCR reaction mix (kk5515, Kapa Biosystems), 1.2 µL of GoScript reverse transcriptase (A5004 Promega), 1 µL of forward primer (10 µM), 1 µL of reverse primer (10 µM), 1 µl of 100 nM SYTO-9 (S34854,

(163) Tentonin 3/TMEM150C senses blood pressure changes in the aortic archThe Journal of clinical investigationJanuary 1, 0001"Lu HJ, Nguyen TL, Hong GS, Pak S, Kim H, Kim H, Kim DY, Kim SY, Shen Y, Ryu PD, et al."Intron, catalog 17221) and reverse transcribed into cDNA using Reverse Transcriptase (GoScript, catalog A5003, Promega). Target genes were amplified by using DreamTaq DNA Polymerases (catalog EP0701, Thermo Fisher Scientific).

(164) PD-L1-directed PlGF/VEGF blockade synergizes with chemotherapy by targeting CD141(+) cancer-associated fibroblasts in pancreatic cancerNature communicationsJanuary 1, 0001"Kim DK, Jeong J, Lee DS, Hyeon DY, Park GW, Jeon S, Lee KB, Jang JY, Hwang D, Kim HM, et al."ng to 1 μg) was reverse-transcribed to cDNA using the GoScriptTM reverse transcriptase kit (Promega, A5003). Following primer validation (Supplementary Table 1), SYBRTM Green PCR Master Mix kit was used to carry

(165) Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balanceAutophagyJanuary 1, 0001"Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo SJ, Lee JW, Moon C, Yu SW, Kim EK."MA, USA). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [50]. SYBR Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(166) GRP78/BiP alleviates oxLDL-induced hepatotoxicity in familial hypercholesterolemia caused by missense variants of LDLR in a HepG2 cellular modelLipids in health and diseaseJanuary 1, 0001"Varghese DS, Oommen D, John A, Ali BR."of 250–500 ng total RNA was done using GoScript Reverse Transcriptase System (Promega, Catalog No. A5004). Relative mRNA expression of target genes was checked using SYBR® Green Real-Time qPCR Master Mix (Thermo Fisher Scientific

(167) "DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction"eLifeJanuary 1, 0001"Lau EO, Damiani D, Chehade G, Ruiz-Reig N, Saade R, Jossin Y, Aittaleb M, Schakman O, Tajeddine N, Gailly P, et al."assay or kit,RNeasy Kit,Qiagen,Cat# 74004, Commercial assay or kit,RT cDNA synthesis Kit,Promega,Cat# A5003, Commercial assay or kit,SYBR Green SuperMix,Biorad,Cat# 170–8882, Commercial assay or kit,Lipofectamine

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