GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomesNucleic Acids ResearchApril 17, 2020Stephan Werner et al.,New England Biolabs #2,AMV,AMV,M0277S,New England Biolabs #3,ProtoScript® II,MMLV,M0368S,New England Biolabs #4,GoScript™,MMLV,A5003,Promega #5,SuperScript™ III,MMLV,18080044,Thermo Fisher Scientific #6,RevertAid™,MMLV,EP0441,Thermo Fisher Scientific

(122) Trypsin activity governs increased susceptibility to pancreatitis in mice expressing human PRSS1R122HThe Journal of clinical investigationJanuary 2, 2020Gui F, Zhang Y, Wan J, Zhan X, Yao Y, Li Y, Haddock AN, Shi J, Guo J, Chen J, et al.(Qiagen). RNA (1 μg) was reverse transcribed to complementary DNA using the Go Script RT kit (catalog A5003; Promega). qPCR was done using PowerUp SYBR Green Master Mix (catalog A25742; Applied Biosystems). Primer

(123) Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemiaPloS oneJanuary 1, 2020Driehuis E, Oosterom N, Heil SG, Muller IB, Lin M, Kolders S, Jansen G, de Jonge R, Pieters R, Clevers H, et al.incubated for 5 minutes at 70˚C. After that, 8.5 GoScript Reverse Transcriptase mastermix (Promega, cat.no. A5003) was added, consisting of RT buffer, MgCl2, dNTPs, RT and RNase inhibitor according to protocol. Samples

(124) Manganese Exposure Aggravates β-Amyloid Pathology by Microglial ActivationFrontiers in aging neuroscienceJanuary 1, 2020Lin G, Li X, Cheng X, Zhao N, Zheng W.The cDNA was synthesized from 2 μg total RNA using the GoScript Reverse Transcription System (Cat.# A5003, Promega, Madison, WI, USA) on the Bio-Rad CFX PCR System. The primer sequences are listed in Table

(125) Ionizable lipid nanoparticles encapsulating barcoded mRNA for accelerated in vivo delivery screeningJournal of controlled release : official journal of the Controlled Release SocietyDecember 28, 2019Guimaraes PPG, Zhang R, Spektor R, Tan M, Chung A, Billingsley MM, El-Mayta R, Riley RS, Wang L, Wilson JM, et al.DNase-treated RNA was carried out in a 20 μL reaction using 1 μL of GoScript Reverse Transcriptase (Promega, A5003) containing a final concentration of 1X GoScript Reaction Buffer, 2.5 mM MgCl2, 0.5 mM dNTPs using the

(126) Pancreatic cancer organoids recapitulate disease and allow personalized drug screeningProceedings of the National Academy of Sciences of the United States of AmericaDecember 26, 2019Driehuis E, van Hoeck A, Moore K, Kolders S, Francies HE, Gulersonmez MC, Stigter ECA, Burgering B, Geurts V, Gracanin A, et al.spectrophotometer (Thermo Fisher Scientific). For cDNA synthesis, GoScript Reverse Transcriptase (Promega; A5003) was used according to protocol. qPCR reactions were performed in 384-well format using IQ SYBR Green (Bio-Rad;

(127) miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9Bioscience ReportsDecember 20, 2019Shenglong Zhao, Jiandong Wang, Zheng Cao, Lei Gao, Yuanyuan Zheng, Jing Wang, Xiaowei LiuFisher Scientific, Inc.) in order to determine the quality of RNA. The GoScript™ Sensiscript RT kit (A5004; Promega Corp.) was used to reversely transcribe the RNA into cDNA. According to the manufacturer’s

(128) Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive PluripotencyStem cell reportsDecember 10, 2019Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, et al.using ReliaPrep Kit (Promega, Z6012) and cDNA synthesized with GoScript reverse transcriptase (Promega, A5004) and oligo(dT) adapter primers. TaqMan assays (Thermo Fisher Scientific) and Universal Probe Library

(129) A putative bHLH transcription factor is a candidate gene for male sterile 32, a locus affecting pollen and tapetum development in tomatoHorticulture ResearchDecember 1, 2019Xiaoyan Liu et al.China), and cDNA was synthesized from 2 μg total RNA using GoScript Reverse Transcriptase (Cat. No. A5003; Promega, Madison, WI, USA). The qPCRs were conducted using GoScriptTM qPCR Master Mix (Cat. No. A6002;

(130) Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegansAgingNovember 22, 2019Xu J, Liu K, Chen T, Zhan T, Ouyang Z, Wang Y, Liu W, Zhang X, Sun Y, Xu G, et al.China). RNA (3 μg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously. The expressed genes were amplified in triplicate and quantified in a SYBR

(131) Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic TherapyJournal of clinical medicineNovember 5, 2019Driehuis E, Spelier S, Beltrán Hernández I, de Bree R, M Willems S, Clevers H, Oliveira S.for 5 min at 70 °C. Subsequently GoScript Reverse Transcriptase (Promega, Madison, WI, USA, cat. no. A5003) was used according to protocol to produce cDNA. qPCR reactions were performed in 384 well format using

(132) Discovery of Druggable Host Factors Critical to Plasmodium Liver-Stage InfectionCell chemical biologySeptember 19, 2019Raphemot R, Toro-Moreno M, Lu KY, Posfai D, Derbyshire ER.04707516001 Quick-RNA™ MicroPrep,Zymo Research,Cat# R1050 GoScript™ Reverse Transcriptase,Promega,Cat# A5003 Deposited Data,Deposited Data,Deposited Data Raw and analyzed data,This paper,N/A Experimental Models:

(133) Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe TomatoesFrontiers in Plant ScienceAugust 29, 2019Yi Chen et al.DNase I (Invitrogen-AM1906), then 2 μg of RNA was treated with GoScript Reverse Transcriptase (Promega-A5003). Quantitative real-time PCR (qPCR) reactions were performed using 5 ng of cDNA per well as described

(134) Increased expression of pgph-1, T23F2.4, and cyp-14A5 in C. elegans dpy-7 mutants and by high saltMicroPubl BiolAugust 29, 2019Gabrielle Scolaro et al.10 min followed by 2 min at 80ºC to denature proteinase K. Promega GoScript Reverse Transcriptase (A5003) was used to synthesize cDNA with poly T primers according to manufacturer’s recommendations. qPCR was

(135) Extracellular vesicles from human bone marrow mesenchymal stem cells repair organ damage caused by cadmium poisoning in a medaka modelPhysiological ReportsJuly 19, 2019Tomomi Matsukura, Chisako Inaba, Esther A. Weygant, Daiki Kitamura, Ralf Janknecht, Hiroyuki Matsumoto, Deborah P. Hyink, Shosaku Kashiwada, Tomoko ObaraRNAqueous‐4® PCR Kit (AM1914, Thermo Fisher). RT‐PCR was performed using GoScript Reverse Transcription (RT) (A5003, Promega) with p(dN)6, followed by a second. PCR using Green Go Taq PCR (M3001, Promega, Madison, WI).

(136) Requirement of Mitochondrial Transcription Factor A in Tissue-Resident Regulatory T Cell Maintenance and FunctionCell reportsJuly 2, 2019Fu Z, Ye J, Dean JW, Bostick JW, Weinberg SE, Xiong L, Oliff KN, Chen ZE, Avram D, Chandel NS, et al.FC-121-1030 NEBNext High-Fidelity 2X PCR Master Mix,NEB,Cat# M0541 GoScript Reverse Transcriptase,Promega,Cat# A5003 iQ SYBR Green Supermix,Bio-Rad,Cat# 1708887 MitoTracker DeepRed,Invitrogen,Cat# M22426 MitoSOX Red,Invitrogen,Cat#

(137) Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer CellsInternational journal of molecular sciencesJuly 2, 2019Hutchinson SA, Lianto P, Moore JB, Hughes TA, Thorne JL.water. Purified RNA was stored at −80 ◦ C. The GoScriptTM Reverse Transcription kit (Promega, Cat: A5003) was used for the cDNA synthesis, and product guidelines followed using 500 ng total RNA/reaction and

(138) The m⁶A pathway protects the transcriptome integrity by restricting RNA chimera formation in plantsLife science allianceJune 1, 2019Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, Llauro C, Azevedo J, Laudié M, Attina A, et al.were obtained from 400 ng of RQ1-treated RNA using 1 μl of GoScript Reverse Transcriptase (Cat. No. A5003; Promega) in a 20-μl final volume reaction using random primers (Cat. No. C1181; Promega) in the presence

(139) The Protein Tyrosine Phosphatase H1 PTPH1 Supports Proliferation of Keratinocytes and is a Target of the Human Papillomavirus Type 8 E6 OncogeneCellsMarch 14, 2019Stefanie Taute, Philipp Böhnke, Jasmin Sprissler, Stephanie Buchholz, Martin Hufbauer, Baki Akgül, Gertrud StegerSpin RNA Plus kit from Macherey-Nagel. RT-PCR was performed with the GoScript Reverse Transcriptase (A5004; Promega) and random primers, and qPCR with the GoTaq-qPCR SYBRGreen master mix (A6002, Promega) and

(140) Mouse and human urothelial cancer organoids: A tool for bladder cancer researchProceedings of the National Academy of Sciences of the United States of AmericaMarch 5, 2019Mullenders J, de Jongh E, Brousali A, Roosen M, Blom JPA, Begthel H, Korving J, Jonges T, Kranenburg O, Meijer R, et al.RNeasy mini kit (Qiagen 74104). The cDNA was prepared using GoScript reverse transcriptase (Promega A5003) using random hexamers (ref). The qRT-PCR was run using Biorad SYBR green supermix (1708886). Primers

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