GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(121) Patient-Derived Head and Neck Cancer Organoids Recapitulate EGFR Expression Levels of Respective Tissues and Are Responsive to EGFR-Targeted Photodynamic TherapyJournal of clinical medicineNovember 5, 2019Driehuis E, Spelier S, Beltrán Hernández I, de Bree R, M Willems S, Clevers H, Oliveira S.for 5 min at 70 °C. Subsequently GoScript Reverse Transcriptase (Promega, Madison, WI, USA, cat. no. A5003) was used according to protocol to produce cDNA. qPCR reactions were performed in 384 well format using

(122) Discovery of Druggable Host Factors Critical to Plasmodium Liver-Stage InfectionCell chemical biologySeptember 19, 2019Raphemot R, Toro-Moreno M, Lu KY, Posfai D, Derbyshire ER.04707516001 Quick-RNA™ MicroPrep,Zymo Research,Cat# R1050 GoScript™ Reverse Transcriptase,Promega,Cat# A5003 Deposited Data,Deposited Data,Deposited Data Raw and analyzed data,This paper,N/A Experimental Models:

(123) Targeted Proteomics Allows Quantification of Ethylene Receptors and Reveals SlETR3 Accumulation in Never-Ripe TomatoesFrontiers in Plant ScienceAugust 29, 2019Yi Chen et al.DNase I (Invitrogen-AM1906), then 2 μg of RNA was treated with GoScript Reverse Transcriptase (Promega-A5003). Quantitative real-time PCR (qPCR) reactions were performed using 5 ng of cDNA per well as described

(124) Increased expression of pgph-1, T23F2.4, and cyp-14A5 in C. elegans dpy-7 mutants and by high saltMicroPubl BiolAugust 29, 2019Gabrielle Scolaro et al.10 min followed by 2 min at 80ºC to denature proteinase K. Promega GoScript Reverse Transcriptase (A5003) was used to synthesize cDNA with poly T primers according to manufacturer’s recommendations. qPCR was

(125) Extracellular vesicles from human bone marrow mesenchymal stem cells repair organ damage caused by cadmium poisoning in a medaka modelPhysiological ReportsJuly 19, 2019Tomomi Matsukura, Chisako Inaba, Esther A. Weygant, Daiki Kitamura, Ralf Janknecht, Hiroyuki Matsumoto, Deborah P. Hyink, Shosaku Kashiwada, Tomoko ObaraRNAqueous‐4® PCR Kit (AM1914, Thermo Fisher). RT‐PCR was performed using GoScript Reverse Transcription (RT) (A5003, Promega) with p(dN)6, followed by a second. PCR using Green Go Taq PCR (M3001, Promega, Madison, WI).

(126) Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer CellsInternational journal of molecular sciencesJuly 2, 2019Hutchinson SA, Lianto P, Moore JB, Hughes TA, Thorne JL.water. Purified RNA was stored at −80 ◦ C. The GoScriptTM Reverse Transcription kit (Promega, Cat: A5003) was used for the cDNA synthesis, and product guidelines followed using 500 ng total RNA/reaction and

(127) Requirement of Mitochondrial Transcription Factor A in Tissue-Resident Regulatory T Cell Maintenance and FunctionCell reportsJuly 2, 2019Fu Z, Ye J, Dean JW, Bostick JW, Weinberg SE, Xiong L, Oliff KN, Chen ZE, Avram D, Chandel NS, et al.FC-121-1030 NEBNext High-Fidelity 2X PCR Master Mix,NEB,Cat# M0541 GoScript Reverse Transcriptase,Promega,Cat# A5003 iQ SYBR Green Supermix,Bio-Rad,Cat# 1708887 MitoTracker DeepRed,Invitrogen,Cat# M22426 MitoSOX Red,Invitrogen,Cat#

(128) The m⁶A pathway protects the transcriptome integrity by restricting RNA chimera formation in plantsLife science allianceJune 1, 2019Pontier D, Picart C, El Baidouri M, Roudier F, Xu T, Lahmy S, Llauro C, Azevedo J, Laudié M, Attina A, et al.were obtained from 400 ng of RQ1-treated RNA using 1 μl of GoScript Reverse Transcriptase (Cat. No. A5003; Promega) in a 20-μl final volume reaction using random primers (Cat. No. C1181; Promega) in the presence

(129) The Protein Tyrosine Phosphatase H1 PTPH1 Supports Proliferation of Keratinocytes and is a Target of the Human Papillomavirus Type 8 E6 OncogeneCellsMarch 14, 2019Stefanie Taute, Philipp Böhnke, Jasmin Sprissler, Stephanie Buchholz, Martin Hufbauer, Baki Akgül, Gertrud StegerSpin RNA Plus kit from Macherey-Nagel. RT-PCR was performed with the GoScript Reverse Transcriptase (A5004; Promega) and random primers, and qPCR with the GoTaq-qPCR SYBRGreen master mix (A6002, Promega) and

(130) Mouse and human urothelial cancer organoids: A tool for bladder cancer researchProceedings of the National Academy of Sciences of the United States of AmericaMarch 5, 2019Mullenders J, de Jongh E, Brousali A, Roosen M, Blom JPA, Begthel H, Korving J, Jonges T, Kranenburg O, Meijer R, et al.RNeasy mini kit (Qiagen 74104). The cDNA was prepared using GoScript reverse transcriptase (Promega A5003) using random hexamers (ref). The qRT-PCR was run using Biorad SYBR green supermix (1708886). Primers

(131) Long-term expanding human airway organoids for disease modelingThe EMBO journalFebruary 15, 2019Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, Heo I, Böttinger L, Klay D, Weeber F, Huelsz-Prince G, Iakobachvili N, Amatngalim GD, et al.Qubit dsDNA HS Assay Kit,Thermo Fisher,Q32854 Illumina Nextseq,Illumina, GoScript Reverse Transcriptase kit,Promega,A5003 QIAquick PCR purification kit,Qiagen,28104 pGEM‐T Easy vector syst. I,Promega,A3600 Nanodrop Lite,Thermo

(132) Why SNP rs227584 is associated with human BMD and fracture risk? A molecular and cellular study in bone cellsJournal of cellular and molecular medicineFebruary 1, 2019Zhou X, Qiu YH, He P, Jiang F, Wu LF, Lu X, Lei SF, Deng FY.cells were lysed and reverse transcribed with GoScript™ Reverse Transcriptase (Promega, Catalogue No.A5003) according to the manufacturer's instructions, respectively. The mRNA expression levels of OPN, COL1A1,

(133) Anti-CRISPR-based biosensors in the yeast S. cerevisiaeJournal of Biological EngineeringDecember 1, 2018Jing Li, Zengliang Xu, Aleksandr Chupalov, Mario Andrea Marchisiosolution were added, into a PCR tube, to a 15 μl “RT mix” (1 μl GoScript Reverse Transcriptase–Promega, A5003; 4 μl GoScript 5X RT buffer; 1.5 μl MgCl2; 4 μl 0.5 mM dNTPmix; nuclease-free water up to the overall

(134) Murine Norovirus Infection Induces T_H1 Inflammatory Responses to Dietary AntigensCell host & microbeNovember 14, 2018Bouziat R, Biering SB, Kouame E, Sangani KA, Kang S, Ernest JD, Varma M, Brown JJ, Urbanek K, Dermody TS, et al.Irf1, Isg15 and S4 Reovirus gene expression, cDNA synthesis was performed using GoScript (Promega, A5004) according to the manufacturer’s instructions. To determine MNV genome copies, cDNA was reverse transcribed

(135) The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphologyNew PhytologistSeptember 1, 2018Rongrong Yuan, Jingqiu Lan, Yuxing Fang, Hao Yu, Jinzhe Zhang, Jiaying Huang, Genji Qinin the RNA purification kit, and was then reverse transcribed using an M‐MLV kit (Promega, cat. no. A5003) in a reaction volume of 20 μl. The cDNA was diluted and used as a template for semi‐quantitative PCR.

(136) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone ResponsesCell stem cellMay 3, 2018Rajamani U, Gross AR, Hjelm BE, Sequeira A, Vawter MP, Tang J, Gangalapudi V, Wang Y, Andres AM, Gottlieb RA, et al.Millipore Human Neuropeptide Y ELISA,EMD Millipore,EZHNPY-25K GoScript Reverse Transcriptase,Promega,A5003 Halt Protease Inhibitor Cocktail,ThermoScientific,78430 Illumina TruSeq stranded mRNA library,Illumina,20020594 Mammalian

(137) Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient hostNatureMay 1, 2018Meisel M, Hinterleitner R, Pacis A, Chen L, Earley ZM, Mayassi T, Pierre JF, Ernest JD, Galipeau HJ, Thuille N, et al.prepared using the RNeasy Mini Kit (Qiagen, 74136). cDNA synthesis was performed using GoScript (Promega, A5004) according to the manufacturer’s instructions. Expression analysis was performed in duplicate via RT–PCR

(138) Rescue of Outer Hair Cells with Antisense Oligonucleotides in Usher Mice Is Dependent on Age of TreatmentJournal of the Association for Research in OtolaryngologyFebruary 1, 2018Abhilash Ponnath, Frederic F. Depreux, Francine M. Jodelka, Frank Rigo, Hamilton E. Farris, Michelle L. Hastings, Jennifer J. Lentz

(139) YAP and TAZ mediate steroid-induced alterations in the trabecular meshwork cytoskeleton in human trabecular meshwork cellsInternational journal of molecular medicineJanuary 1, 2018Peng J, Wang H, Wang X, Sun M, Deng S, Wang Y.Thermo Fisher Scientific, Inc.), 1X reverse transcription buffer and 10 units reverse transcriptase (A5003; Promega, Madison, WI, USA) in a total volume of 20 μl. The RNA and primer were heated to 72°C and slowly

(140) A putative R3 MYB repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruitJournal of Experimental BotanyDecember 16, 2017Xue Cao et al.China), and cDNA was synthesized from 1 μg total RNA by using GoScriptTM Reverse Transcriptase (Cat. No. A5003, Promega, USA). Real-time quantitative PCR was conducted by using the GoScriptTM qPCR Master Mix (Cat.

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