GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) Long-term expanding human airway organoids for disease modelingThe EMBO journalFebruary 15, 2019Sachs N, Papaspyropoulos A, Zomer-van Ommen DD, Heo I, Böttinger L, Klay D, Weeber F, Huelsz-Prince G, Iakobachvili N, Amatngalim GD, et al.Qubit dsDNA HS Assay Kit,Thermo Fisher,Q32854 Illumina Nextseq,Illumina, GoScript Reverse Transcriptase kit,Promega,A5003 QIAquick PCR purification kit,Qiagen,28104 pGEM‐T Easy vector syst. I,Promega,A3600 Nanodrop Lite,Thermo

(142) Why SNP rs227584 is associated with human BMD and fracture risk? A molecular and cellular study in bone cellsJournal of cellular and molecular medicineFebruary 1, 2019Zhou X, Qiu YH, He P, Jiang F, Wu LF, Lu X, Lei SF, Deng FY.cells were lysed and reverse transcribed with GoScript™ Reverse Transcriptase (Promega, Catalogue No.A5003) according to the manufacturer's instructions, respectively. The mRNA expression levels of OPN, COL1A1,

(143) Anti-CRISPR-based biosensors in the yeast S. cerevisiaeJournal of Biological EngineeringDecember 1, 2018Jing Li, Zengliang Xu, Aleksandr Chupalov, Mario Andrea Marchisiosolution were added, into a PCR tube, to a 15 μl “RT mix” (1 μl GoScript Reverse Transcriptase–Promega, A5003; 4 μl GoScript 5X RT buffer; 1.5 μl MgCl2; 4 μl 0.5 mM dNTPmix; nuclease-free water up to the overall

(144) Murine Norovirus Infection Induces T_H1 Inflammatory Responses to Dietary AntigensCell host & microbeNovember 14, 2018Bouziat R, Biering SB, Kouame E, Sangani KA, Kang S, Ernest JD, Varma M, Brown JJ, Urbanek K, Dermody TS, et al.Irf1, Isg15 and S4 Reovirus gene expression, cDNA synthesis was performed using GoScript (Promega, A5004) according to the manufacturer’s instructions. To determine MNV genome copies, cDNA was reverse transcribed

(145) The Arabidopsis USL1 controls multiple aspects of development by affecting late endosome morphologyNew PhytologistSeptember 1, 2018Rongrong Yuan, Jingqiu Lan, Yuxing Fang, Hao Yu, Jinzhe Zhang, Jiaying Huang, Genji Qinin the RNA purification kit, and was then reverse transcribed using an M‐MLV kit (Promega, cat. no. A5003) in a reaction volume of 20 μl. The cDNA was diluted and used as a template for semi‐quantitative PCR.

(146) Super-Obese Patient-Derived iPSC Hypothalamic Neurons Exhibit Obesogenic Signatures and Hormone ResponsesCell stem cellMay 3, 2018Rajamani U, Gross AR, Hjelm BE, Sequeira A, Vawter MP, Tang J, Gangalapudi V, Wang Y, Andres AM, Gottlieb RA, et al.Millipore Human Neuropeptide Y ELISA,EMD Millipore,EZHNPY-25K GoScript Reverse Transcriptase,Promega,A5003 Halt Protease Inhibitor Cocktail,ThermoScientific,78430 Illumina TruSeq stranded mRNA library,Illumina,20020594 Mammalian

(147) Microbial signals drive pre-leukaemic myeloproliferation in a Tet2-deficient hostNatureMay 1, 2018Meisel M, Hinterleitner R, Pacis A, Chen L, Earley ZM, Mayassi T, Pierre JF, Ernest JD, Galipeau HJ, Thuille N, et al.prepared using the RNeasy Mini Kit (Qiagen, 74136). cDNA synthesis was performed using GoScript (Promega, A5004) according to the manufacturer’s instructions. Expression analysis was performed in duplicate via RT–PCR

(148) Rescue of Outer Hair Cells with Antisense Oligonucleotides in Usher Mice Is Dependent on Age of TreatmentJournal of the Association for Research in OtolaryngologyFebruary 1, 2018Abhilash Ponnath, Frederic F. Depreux, Francine M. Jodelka, Frank Rigo, Hamilton E. Farris, Michelle L. Hastings, Jennifer J. Lentz

(149) YAP and TAZ mediate steroid-induced alterations in the trabecular meshwork cytoskeleton in human trabecular meshwork cellsInternational journal of molecular medicineJanuary 1, 2018Peng J, Wang H, Wang X, Sun M, Deng S, Wang Y.Thermo Fisher Scientific, Inc.), 1X reverse transcription buffer and 10 units reverse transcriptase (A5003; Promega, Madison, WI, USA) in a total volume of 20 μl. The RNA and primer were heated to 72°C and slowly

(150) A putative R3 MYB repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruitJournal of Experimental BotanyDecember 16, 2017Xue Cao et al.China), and cDNA was synthesized from 1 μg total RNA by using GoScriptTM Reverse Transcriptase (Cat. No. A5003, Promega, USA). Real-time quantitative PCR was conducted by using the GoScriptTM qPCR Master Mix (Cat.

(151) The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coliBiomoleculesOctober 31, 2017Daniel R. Smith, Janet E. Price, Peter E. Burby, Luz P. Blanco, Justin Chamberlain, Matthew R. ChapmanQuantitative PCR Reverse transcriptase complementary DNA (cDNA) synthesis was performed using Promega Go-Script (A5003) (Promega, Madison, WI, USA) according to the manufacturer’s instructions with random primers (Invitrogen,

(152) Nuclear factor-κB signaling negatively regulates high glucose-induced vascular endothelial cell damage downstream of the extracellular signal-regulated kinase/c-Jun N-terminal kinase pathwayExperimental and Therapeutic MedicineOctober 1, 2017Yunzhi Chen, Fang Guo, Zheng Ru, Hongru Kong, Hongwei Sun, Huajun Yu, Wenjun Yang, Qiyu Zhang, Mengtao Zhouchloroform was added. Total RNA (1 µg) was reverse‑transcribed by using a GoScript Reverse Transcription kit (A5003; Promega Corp., Madison, WI, USA) following the manufacturer's instructions. The kit included Buffer,

(153) Functional evaluation of a PTSD-associated genetic variant: estradiol regulation and ADCYAP1R1Translational PsychiatryDecember 1, 2016K B Mercer, B Dias, D Shafer, S A Maddox, J G Mulle, P Hu, J Walton, K J ResslerReverse transcriptase PCR reactions were performed with 2 μl of total RNA and GoScript Reverse Transcriptase (catalog no. A5003, Promega, Madison, WI, USA), according to the manufacturer's protocol. qPCR reactions were performed

(154) Anterior Gradient 2 (AGR2) Induced Epidermal Growth Factor Receptor (EGFR) Signaling Is Essential for Murine Pancreatitis-Associated Tissue RegenerationPLOS ONEOctober 20, 2016Dariusz Wodziak, Aiwen Dong, Michael F. Basin, Anson W. Loweperformed with 3 μg of RNA reverse transcribed with GoScript Reverse Transcriptase System (Promega, A5003) to generate cDNA, which was subjected to SYBR Green-based real-time PCR analysis with an iCycler iQ™

(155) Organoid culture systems for prostate epithelial and cancer tissueNature protocolsFebruary 1, 2016Drost J, Karthaus WR, Gao D, Driehuis E, Sawyers CL, Chen Y, Clevers H.Reliaprep gDNA tissue miniprep system (Promega, cat. no. A2052) GoScript Reverse Transcriptase (Promega, cat. no. A5003) Oligo(dT) 15 Primer (Promega, cat. no. C1101) Rec. RNasin RNase Inhibitor (Promega, cat. no. N2511) Mouse

(156) Histone demethylase JMJD2A drives prostate tumorigenesis through transcription factor ETV1Journal of Clinical InvestigationJanuary 5, 2016Tae-Dong Kim, Fang Jin, Sook Shin, Sangphil Oh, Stan A. Lightfoot, Joseph P. Grande, Aaron J. Johnson, Jan M. van Deursen, Jonathan D. Wren, Ralf Janknecht(72), and approximately 1 μg RNA was reverse transcribed using GoScript reverse transcriptase (Promega, A5004) and pd(N)6 random primers (Roche Applied Science, 11034731001) following the manufacturer’s instructions.

(157) Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafishProceedings of the Royal Society B: Biological SciencesAugust 7, 2015Yohey Ogawa, Tomoya Shiraki, Daisuke Kojima, Yoshitaka Fukadaeye) was reverse-transcribed into cDNA using the oligo (dT)15 primer with GoScript Reverse Transcriptase (A5003, Promega). The cDNA was subjected to quantitative PCR using GoTaq qPCR Master Mix (A6001, Promega) with

(158) Apelin Receptor (APJ) Expression during Cardiopulmonary Bypass in Children Undergoing Surgical RepairPLoS ONESeptember 3, 2014Susan Walker, Mark H. D. Danton, Alexander D. Lang, Fiona LyallQuantiTect Reverse transcription Kit (Qiagen, 205310) and GoScript reverse transcriptase (Promega, A5003) the RNA was reverse transcribed into cDNA. The products of the PCR reaction were separated on a 2%

(159) Use of surface enhanced blocking (SEB) electrodes for microbial cell lysis in flow-through devicesPloS oneJanuary 1, 2014Abdossamad Talebpour, Robert Maaskant, Aye Aye Khine, Tino AlavieRobust HotStart 2X PCR reaction mix (kk5515, Kapa Biosystems), 1.2 µL of GoScript reverse transcriptase (A5004 Promega), 1 µL of forward primer (10 µM), 1 µL of reverse primer (10 µM), 1 µl of 100 nM SYTO-9 (S34854,

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