GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(141) The Production of Curli Amyloid Fibers Is Deeply Integrated into the Biology of Escherichia coliBiomoleculesOctober 31, 2017Daniel R. Smith, Janet E. Price, Peter E. Burby, Luz P. Blanco, Justin Chamberlain, Matthew R. ChapmanQuantitative PCR Reverse transcriptase complementary DNA (cDNA) synthesis was performed using Promega Go-Script (A5003) (Promega, Madison, WI, USA) according to the manufacturer’s instructions with random primers (Invitrogen,

(142) Nuclear factor-κB signaling negatively regulates high glucose-induced vascular endothelial cell damage downstream of the extracellular signal-regulated kinase/c-Jun N-terminal kinase pathwayExperimental and Therapeutic MedicineOctober 1, 2017Yunzhi Chen, Fang Guo, Zheng Ru, Hongru Kong, Hongwei Sun, Huajun Yu, Wenjun Yang, Qiyu Zhang, Mengtao Zhouchloroform was added. Total RNA (1 µg) was reverse‑transcribed by using a GoScript Reverse Transcription kit (A5003; Promega Corp., Madison, WI, USA) following the manufacturer's instructions. The kit included Buffer,

(143) Functional evaluation of a PTSD-associated genetic variant: estradiol regulation and ADCYAP1R1Translational PsychiatryDecember 1, 2016K B Mercer, B Dias, D Shafer, S A Maddox, J G Mulle, P Hu, J Walton, K J ResslerReverse transcriptase PCR reactions were performed with 2 μl of total RNA and GoScript Reverse Transcriptase (catalog no. A5003, Promega, Madison, WI, USA), according to the manufacturer's protocol. qPCR reactions were performed

(144) Anterior Gradient 2 (AGR2) Induced Epidermal Growth Factor Receptor (EGFR) Signaling Is Essential for Murine Pancreatitis-Associated Tissue RegenerationPLOS ONEOctober 20, 2016Dariusz Wodziak, Aiwen Dong, Michael F. Basin, Anson W. Loweperformed with 3 μg of RNA reverse transcribed with GoScript Reverse Transcriptase System (Promega, A5003) to generate cDNA, which was subjected to SYBR Green-based real-time PCR analysis with an iCycler iQ™

(145) Organoid culture systems for prostate epithelial and cancer tissueNature protocolsFebruary 1, 2016Drost J, Karthaus WR, Gao D, Driehuis E, Sawyers CL, Chen Y, Clevers H.Reliaprep gDNA tissue miniprep system (Promega, cat. no. A2052) GoScript Reverse Transcriptase (Promega, cat. no. A5003) Oligo(dT) 15 Primer (Promega, cat. no. C1101) Rec. RNasin RNase Inhibitor (Promega, cat. no. N2511) Mouse

(146) Histone demethylase JMJD2A drives prostate tumorigenesis through transcription factor ETV1Journal of Clinical InvestigationJanuary 5, 2016Tae-Dong Kim, Fang Jin, Sook Shin, Sangphil Oh, Stan A. Lightfoot, Joseph P. Grande, Aaron J. Johnson, Jan M. van Deursen, Jonathan D. Wren, Ralf Janknecht(72), and approximately 1 μg RNA was reverse transcribed using GoScript reverse transcriptase (Promega, A5004) and pd(N)6 random primers (Roche Applied Science, 11034731001) following the manufacturer’s instructions.

(147) Homeobox transcription factor Six7 governs expression of green opsin genes in zebrafishProceedings of the Royal Society B: Biological SciencesAugust 7, 2015Yohey Ogawa, Tomoya Shiraki, Daisuke Kojima, Yoshitaka Fukadaeye) was reverse-transcribed into cDNA using the oligo (dT)15 primer with GoScript Reverse Transcriptase (A5003, Promega). The cDNA was subjected to quantitative PCR using GoTaq qPCR Master Mix (A6001, Promega) with

(148) Apelin Receptor (APJ) Expression during Cardiopulmonary Bypass in Children Undergoing Surgical RepairPLoS ONESeptember 3, 2014Susan Walker, Mark H. D. Danton, Alexander D. Lang, Fiona LyallQuantiTect Reverse transcription Kit (Qiagen, 205310) and GoScript reverse transcriptase (Promega, A5003) the RNA was reverse transcribed into cDNA. The products of the PCR reaction were separated on a 2%

(149) Use of surface enhanced blocking (SEB) electrodes for microbial cell lysis in flow-through devicesPloS oneJanuary 1, 2014Abdossamad Talebpour, Robert Maaskant, Aye Aye Khine, Tino AlavieRobust HotStart 2X PCR reaction mix (kk5515, Kapa Biosystems), 1.2 µL of GoScript reverse transcriptase (A5004 Promega), 1 µL of forward primer (10 µM), 1 µL of reverse primer (10 µM), 1 µl of 100 nM SYTO-9 (S34854,

Be the First to Write a Review!