GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

Add to Compare List

Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and DucksFrontiers in microbiologyJanuary 1, 2021Wang B, Su Q, Luo J, Li M, Wu Q, Chang H, Du J, Huang C, Ma J, Han S, et al.reversetranscribed using primer Uni12 and oligo-dT and the GoScript™ Reverse Transcriptase System (Promega, A5004). Quantitative real-time polymerase chain reactions (qRT-PCRs) targeting the influenza virus M gene

(102) CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in Nicotiana tabacumFrontiers in Genome EditingDecember 23, 2020Sophie Donovan, Yuwei Mao, Douglas J. Orr, Elizabete Carmo-Silva, Alistair J. McCormickreverse-transcribed in a 20 μL reaction according to the protocol for the GoScript Reverse Transcription System (A5003, Promega, USA). Quantitative reverse transcription PCR (qRT-PCR) reactions were prepared in a 10 μL

(103) Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cellsScientific ReportsDecember 1, 2020Hee-Sun Yang, Hyo Jung Sim, Hanna Cho, Woo Young Bang, Ha Eun Kim, Taeg Kyu Kwon, Taejoon Kwon, Tae Joo Parkrate (FDR). Quantitative PCR cDNA was synthesized using GoScript Reverse Transcriptase (Promega, A5003) according to manufacturer’s protocol. Real-time PCR was performed using PowerUp SYBR Green Master Mix

(104) Inhibition of IL-1beta improves Glycaemia in a Mouse Model for Gestational DiabetesScientific ReportsDecember 1, 2020Friederike Schulze et al.instructions. Complementary deoxyribonucleic acid was prepared using the GoScript Reverse Transcriptase (A5003, Promega, Catalys, Switzerland) and used for Taqman qPCR or Sybr Green qPCR on a ViiA 7 real-time PCR system

(105) LncRNA SNHG10 increases the methylation of miR-218 gene to promote glucose uptake and cell proliferation in osteosarcomaJournal of Orthopaedic Surgery and ResearchDecember 1, 2020Pan He, Yongqiang Xu, Zhijun Wangclose to 2.0 were subjected to reverse transcriptions (RTs) using GoScript™ Reverse Transcriptase kit (A5003, Promega Corporation). With cDNA samples as template, qPCRs were performed using GeneRead qPCR SYBR

(106) SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancersScientific reportsSeptember 3, 2020Jang BG, Kim HS, Bae JM, Kim WH, Kim HU, Kang GH.oligo-dT primers and the GoScript reverse transcription system (Promega, Madison, WI, catalog number: A5004) to generate complementary DNA (cDNA). Subsequently, cDNA was used to perform real-time PCR with Premix

(107) SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant HeightFrontiers in GeneticsAugust 7, 2020Xiaolin Liu et al.Analysis cDNA was synthesized from 2 μg total RNA using GoScriptTM Reverse Transcriptase (Cat. No. A5003; Promega, Madison, WI, United States). The qPCR reactions were conducted using the GoScriptTM qPCR Master Mix

(108) lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic NephropathyDiabetes, Metabolic Syndrome and Obesity: Targets and TherapyJuly 10, 2020Jinmei Zhang, Liwen Song, Yanjuan Ma, Yan Yin, Xinqi Liu, Xinyu Luo, Jiali Sun, Liqin WangDNAs. RNA integrity was checked using a Urea-PAGE gel (6%). RT-qPCR A reverse transcription system (A5003, Promega Corporation) was used to reverse transcribe RNA samples into cDNA samples. The qPCRs reactions

(109) Tentonin 3/TMEM150C senses blood pressure changes in the aortic archThe Journal of clinical investigationJuly 1, 2020Lu HJ, Nguyen TL, Hong GS, Pak S, Kim H, Kim H, Kim DY, Kim SY, Shen Y, Ryu PD, et al.Intron, catalog 17221) and reverse transcribed into cDNA using Reverse Transcriptase (GoScript, catalog A5003, Promega). Target genes were amplified by using DreamTaq DNA Polymerases (catalog EP0701, Thermo Fisher Scientific).

(110) Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balanceAutophagyJuly 1, 2020Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo SJ, Lee JW, Moon C, Yu SW, Kim EK.MA, USA). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [50]. SYBR Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(111) Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomesNucleic Acids ResearchApril 17, 2020Stephan Werner et al.,New England Biolabs #2,AMV,AMV,M0277S,New England Biolabs #3,ProtoScript® II,MMLV,M0368S,New England Biolabs #4,GoScript™,MMLV,A5003,Promega #5,SuperScript™ III,MMLV,18080044,Thermo Fisher Scientific #6,RevertAid™,MMLV,EP0441,Thermo Fisher Scientific

(112) Trypsin activity governs increased susceptibility to pancreatitis in mice expressing human PRSS1R122HThe Journal of clinical investigationJanuary 2, 2020Gui F, Zhang Y, Wan J, Zhan X, Yao Y, Li Y, Haddock AN, Shi J, Guo J, Chen J, et al.(Qiagen). RNA (1 μg) was reverse transcribed to complementary DNA using the Go Script RT kit (catalog A5003; Promega). qPCR was done using PowerUp SYBR Green Master Mix (catalog A25742; Applied Biosystems). Primer

(113) Manganese Exposure Aggravates β-Amyloid Pathology by Microglial ActivationFrontiers in aging neuroscienceJanuary 1, 2020Lin G, Li X, Cheng X, Zhao N, Zheng W.The cDNA was synthesized from 2 μg total RNA using the GoScript Reverse Transcription System (Cat.# A5003, Promega, Madison, WI, USA) on the Bio-Rad CFX PCR System. The primer sequences are listed in Table

(114) Patient-derived oral mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemiaPloS oneJanuary 1, 2020Driehuis E, Oosterom N, Heil SG, Muller IB, Lin M, Kolders S, Jansen G, de Jonge R, Pieters R, Clevers H, et al.incubated for 5 minutes at 70˚C. After that, 8.5 GoScript Reverse Transcriptase mastermix (Promega, cat.no. A5003) was added, consisting of RT buffer, MgCl2, dNTPs, RT and RNase inhibitor according to protocol. Samples

(115) Ionizable lipid nanoparticles encapsulating barcoded mRNA for accelerated in vivo delivery screeningJournal of controlled release : official journal of the Controlled Release SocietyDecember 28, 2019Guimaraes PPG, Zhang R, Spektor R, Tan M, Chung A, Billingsley MM, El-Mayta R, Riley RS, Wang L, Wilson JM, et al.DNase-treated RNA was carried out in a 20 μL reaction using 1 μL of GoScript Reverse Transcriptase (Promega, A5003) containing a final concentration of 1X GoScript Reaction Buffer, 2.5 mM MgCl2, 0.5 mM dNTPs using the

(116) Pancreatic cancer organoids recapitulate disease and allow personalized drug screeningProceedings of the National Academy of Sciences of the United States of AmericaDecember 26, 2019Driehuis E, van Hoeck A, Moore K, Kolders S, Francies HE, Gulersonmez MC, Stigter ECA, Burgering B, Geurts V, Gracanin A, et al.spectrophotometer (Thermo Fisher Scientific). For cDNA synthesis, GoScript Reverse Transcriptase (Promega; A5003) was used according to protocol. qPCR reactions were performed in 384-well format using IQ SYBR Green (Bio-Rad;

(117) miR-126a-3p induces proliferation, migration and invasion of trophoblast cells in pre-eclampsia-like rats by inhibiting A Disintegrin and Metalloprotease 9Bioscience ReportsDecember 20, 2019Shenglong Zhao, Jiandong Wang, Zheng Cao, Lei Gao, Yuanyuan Zheng, Jing Wang, Xiaowei LiuFisher Scientific, Inc.) in order to determine the quality of RNA. The GoScript™ Sensiscript RT kit (A5004; Promega Corp.) was used to reversely transcribe the RNA into cDNA. According to the manufacturer’s

(118) Wnt Inhibition Facilitates RNA-Mediated Reprogramming of Human Somatic Cells to Naive PluripotencyStem cell reportsDecember 10, 2019Bredenkamp N, Yang J, Clarke J, Stirparo GG, von Meyenn F, Dietmann S, Baker D, Drummond R, Ren Y, Li D, et al.using ReliaPrep Kit (Promega, Z6012) and cDNA synthesized with GoScript reverse transcriptase (Promega, A5004) and oligo(dT) adapter primers. TaqMan assays (Thermo Fisher Scientific) and Universal Probe Library

(119) A putative bHLH transcription factor is a candidate gene for male sterile 32, a locus affecting pollen and tapetum development in tomatoHorticulture ResearchDecember 1, 2019Xiaoyan Liu et al.China), and cDNA was synthesized from 2 μg total RNA using GoScript Reverse Transcriptase (Cat. No. A5003; Promega, Madison, WI, USA). The qPCRs were conducted using GoScriptTM qPCR Master Mix (Cat. No. A6002;

(120) Rotating magnetic field delays human umbilical vein endothelial cell aging and prolongs the lifespan of Caenorhabditis elegansAgingNovember 22, 2019Xu J, Liu K, Chen T, Zhan T, Ouyang Z, Wang Y, Liu W, Zhang X, Sun Y, Xu G, et al.China). RNA (3 μg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously. The expressed genes were amplified in triplicate and quantified in a SYBR

Be the First to Write a Review!