GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) Modelling of primary ciliary dyskinesia using patient-derived airway organoidsEMBO reportsDecember 6, 2021van der Vaart J, Böttinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, et al.RNeasy Mini Kit according to protocol. For cDNA synthesis, GoScript Reverse Transcriptase (Promega; A5003) was used according to protocol. qPCRs were performed in 384‐well format using IQ SYBR Green (Bio‐Rad;

(102) Characterization and prognostic value of LXR splice variants in triple-negative breast canceriScienceOctober 22, 2021Lianto P, Hutchinson SA, Moore JB, Hughes TA, Thorne JL.& Nuclear RNA Purification Kit,Norgen,Cat. 21000 GoScriptTM Reverse Transcription kit,Promega,Cat. A5003 BCA kit,Thermo Fisher,Cat. 23227 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(103) HIV-1 Reverse Transcriptase Inhibition by Major Compounds in a Kenyan Multi-Herbal Composition (CareVid™): In Vitro and In Silico ContrastPharmaceuticals (Basel)September 30, 2021Winnie Rotich, Nicholas J. Sadgrove, Eduard Mas-Claret, Guillermo F. Padilla-González, Anastasia Guantai, Moses K. Langattranscriptase was purchased from Promega (Madison, WI, USA) (GoScript™ Reverse Transcriptase: catalogue number A5004) and the enzyme dilution buffer from Thermo Fisher (Waltham, MA, USA) (catalogue number B19). Briefly,

(104) Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infectionPhysiological ReportsSeptember 20, 2021Loretta Müller, Jakob Usemann, Marco P. Alves, Philipp LatzincDNA synthesis (using 200 ng of total RNA and the GoScript Reverse Transcription System; Promega, cat.no. A5003), quantitative real‐time RT‐PCR (384‐well plates, duplicates for target genes, quadruplicates for the

(105) Impact on Bile Acid Concentrations by Alveolar Echinococcosis and Treatment with Albendazole in MiceMetabolitesJuly 6, 2021Cristina Gómez, Fadi Jebbawi, Michael Weingartner, Junhua Wang, Simon Stücheli, Bruno Stieger, Bruno Gottstein, Guido Beldi, Britta Lundström-Stadelmann, Alex Odermatt1.5 and 2.0 were further processed. cDNA was synthesized using GoScript Reverse Transcriptase (Cat#A5003, Promega, Madison, WI, USA). The KAPA SYBR Fast Kit (Cat# SFUKB, Merck, Darmstadt, Germany) was used

(106) Human naive epiblast cells possess unrestricted lineage potentialCell stem cellJune 3, 2021Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al.using ReliaPrep kit (Promega, Z6012) and cDNA synthesized with GoScript reverse transcriptase (Promega, A5004) and oligo(dT) adaptor primers. TaqMan assays (Thermo Fisher Scientific) and Universal Probe Library

(107) A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damageCell reportsMay 11, 2021Gambino F Jr, Tai W, Voronin D, Zhang Y, Zhang X, Shi J, Wang X, Wang N, Du L, Qiao L.Kit,QIAGEN,27104 RNeasy Mini Prep Kit,QIAGEN,74104 GoScript Reverse Transcription System,Promega,A5003 iTaq Universal SYBRGreen Supermix,Bio-Rad,1725121 Experimental models: cell lines,Experimental models:

(108) Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophyActa neuropathologica communicationsApril 14, 2021Mészáros L, Riemenschneider MJ, Gassner H, Marxreiter F, von Hörsten S, Hoffmann A, Winkler J.concentration was measured using Nano-Drop technology (PeqLab). GoScript™ Reverse Transcription System (A5004, Promega) was applied for cDNA production. To quantify gene transcription, qPCRs were performed on a

(109) Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cellsMolecular brainApril 6, 2021Lim Y, Kim S, Kim EK.Scientific). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [34]. TB Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(110) Expanding the genetic spectrum of primary familial brain calcification due to SLC2OA2 mutations: a case seriesneurogeneticsMarch 1, 2021Luca Magistrelli et al.recommendation. Total RNA was used to produce cDNA using the GoScript™ Reverse Transcriptase (Cat# A5003, Promega). SLC20A2 cDNA was amplified using the following primers: forward 5′-CCGTGTTACTAGGCGCCAAAGTAG-3′,

(111) LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationCancer Manag ResFebruary 10, 2021Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhangamong 1.8–2.0, which indicated the high quality of RNA. RT-qPCR Assay A Reverse Transcription System (A5003, Promega Corporation) was used to reverse transcribe RNA samples (from both in vitro cultivated cells

(112) LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationCancer Management and ResearchFebruary 10, 2021Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhangamong 1.8–2.0, which indicated the high quality of RNA. RT-qPCR Assay A Reverse Transcription System (A5003, Promega Corporation) was used to reverse transcribe RNA samples (from both in vitro cultivated cells

(113) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-AriasRT) References (1) Promega (https://www.promega.es/products/pcr/rt‐pcr/goscript‐reverse‐ transcriptase/?catNum=A5003) (2) Bagnoli, J.W. et al. (2018). Sensitive and powerful single‐cell RNA sequencing using mcSCRB‐ seq.

(114) Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and DucksFrontiers in microbiologyJanuary 1, 2021Wang B, Su Q, Luo J, Li M, Wu Q, Chang H, Du J, Huang C, Ma J, Han S, et al.reversetranscribed using primer Uni12 and oligo-dT and the GoScript™ Reverse Transcriptase System (Promega, A5004). Quantitative real-time polymerase chain reactions (qRT-PCRs) targeting the influenza virus M gene

(115) CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in Nicotiana tabacumFrontiers in Genome EditingDecember 23, 2020Sophie Donovan, Yuwei Mao, Douglas J. Orr, Elizabete Carmo-Silva, Alistair J. McCormickreverse-transcribed in a 20 μL reaction according to the protocol for the GoScript Reverse Transcription System (A5003, Promega, USA). Quantitative reverse transcription PCR (qRT-PCR) reactions were prepared in a 10 μL

(116) Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cellsScientific ReportsDecember 1, 2020Hee-Sun Yang, Hyo Jung Sim, Hanna Cho, Woo Young Bang, Ha Eun Kim, Taeg Kyu Kwon, Taejoon Kwon, Tae Joo Parkrate (FDR). Quantitative PCR cDNA was synthesized using GoScript Reverse Transcriptase (Promega, A5003) according to manufacturer’s protocol. Real-time PCR was performed using PowerUp SYBR Green Master Mix

(117) LncRNA SNHG10 increases the methylation of miR-218 gene to promote glucose uptake and cell proliferation in osteosarcomaJournal of Orthopaedic Surgery and ResearchDecember 1, 2020Pan He, Yongqiang Xu, Zhijun Wangclose to 2.0 were subjected to reverse transcriptions (RTs) using GoScript™ Reverse Transcriptase kit (A5003, Promega Corporation). With cDNA samples as template, qPCRs were performed using GeneRead qPCR SYBR

(118) Inhibition of IL-1beta improves Glycaemia in a Mouse Model for Gestational DiabetesScientific ReportsDecember 1, 2020Friederike Schulze et al.instructions. Complementary deoxyribonucleic acid was prepared using the GoScript Reverse Transcriptase (A5003, Promega, Catalys, Switzerland) and used for Taqman qPCR or Sybr Green qPCR on a ViiA 7 real-time PCR system

(119) SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancersScientific reportsSeptember 3, 2020Jang BG, Kim HS, Bae JM, Kim WH, Kim HU, Kang GH.oligo-dT primers and the GoScript reverse transcription system (Promega, Madison, WI, catalog number: A5004) to generate complementary DNA (cDNA). Subsequently, cDNA was used to perform real-time PCR with Premix

(120) SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant HeightFrontiers in GeneticsAugust 7, 2020Xiaolin Liu et al.Analysis cDNA was synthesized from 2 μg total RNA using GoScriptTM Reverse Transcriptase (Cat. No. A5003; Promega, Madison, WI, United States). The qPCR reactions were conducted using the GoScriptTM qPCR Master Mix

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