GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5004
Quantity500 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(101) DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunctioneLifeApril 26, 2021Lau EO, Damiani D, Chehade G, Ruiz-Reig N, Saade R, Jossin Y, Aittaleb M, Schakman O, Tajeddine N, Gailly P, et al.assay or kit,RNeasy Kit,Qiagen,Cat# 74004, Commercial assay or kit,RT cDNA synthesis Kit,Promega,Cat# A5003, Commercial assay or kit,SYBR Green SuperMix,Biorad,Cat# 170–8882, Commercial assay or kit,Lipofectamine

(102) Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophyActa neuropathologica communicationsApril 14, 2021Mészáros L, Riemenschneider MJ, Gassner H, Marxreiter F, von Hörsten S, Hoffmann A, Winkler J.concentration was measured using Nano-Drop technology (PeqLab). GoScript™ Reverse Transcription System (A5004, Promega) was applied for cDNA production. To quantify gene transcription, qPCRs were performed on a

(103) Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cellsMolecular brainApril 6, 2021Lim Y, Kim S, Kim EK.Scientific). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [34]. TB Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(104) Expanding the genetic spectrum of primary familial brain calcification due to SLC2OA2 mutations: a case seriesneurogeneticsMarch 1, 2021Luca Magistrelli et al.recommendation. Total RNA was used to produce cDNA using the GoScript™ Reverse Transcriptase (Cat# A5003, Promega). SLC20A2 cDNA was amplified using the following primers: forward 5′-CCGTGTTACTAGGCGCCAAAGTAG-3′,

(105) LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationCancer Manag ResFebruary 10, 2021Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhangamong 1.8–2.0, which indicated the high quality of RNA. RT-qPCR Assay A Reverse Transcription System (A5003, Promega Corporation) was used to reverse transcribe RNA samples (from both in vitro cultivated cells

(106) LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationCancer Management and ResearchFebruary 10, 2021Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhangamong 1.8–2.0, which indicated the high quality of RNA. RT-qPCR Assay A Reverse Transcription System (A5003, Promega Corporation) was used to reverse transcribe RNA samples (from both in vitro cultivated cells

(107) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-AriasRT) References (1) Promega (https://www.promega.es/products/pcr/rt‐pcr/goscript‐reverse‐ transcriptase/?catNum=A5003) (2) Bagnoli, J.W. et al. (2018). Sensitive and powerful single‐cell RNA sequencing using mcSCRB‐ seq.

(108) Differences in Highly Pathogenic H5N6 Avian Influenza Viral Pathogenicity and Inflammatory Response in Chickens and DucksFrontiers in microbiologyJanuary 1, 2021Wang B, Su Q, Luo J, Li M, Wu Q, Chang H, Du J, Huang C, Ma J, Han S, et al.reversetranscribed using primer Uni12 and oligo-dT and the GoScript™ Reverse Transcriptase System (Promega, A5004). Quantitative real-time polymerase chain reactions (qRT-PCRs) targeting the influenza virus M gene

(109) CRISPR-Cas9-Mediated Mutagenesis of the Rubisco Small Subunit Family in Nicotiana tabacumFrontiers in Genome EditingDecember 23, 2020Sophie Donovan, Yuwei Mao, Douglas J. Orr, Elizabete Carmo-Silva, Alistair J. McCormickreverse-transcribed in a 20 μL reaction according to the protocol for the GoScript Reverse Transcription System (A5003, Promega, USA). Quantitative reverse transcription PCR (qRT-PCR) reactions were prepared in a 10 μL

(110) Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cellsScientific ReportsDecember 1, 2020Hee-Sun Yang, Hyo Jung Sim, Hanna Cho, Woo Young Bang, Ha Eun Kim, Taeg Kyu Kwon, Taejoon Kwon, Tae Joo Parkrate (FDR). Quantitative PCR cDNA was synthesized using GoScript Reverse Transcriptase (Promega, A5003) according to manufacturer’s protocol. Real-time PCR was performed using PowerUp SYBR Green Master Mix

(111) Inhibition of IL-1beta improves Glycaemia in a Mouse Model for Gestational DiabetesScientific ReportsDecember 1, 2020Friederike Schulze et al.instructions. Complementary deoxyribonucleic acid was prepared using the GoScript Reverse Transcriptase (A5003, Promega, Catalys, Switzerland) and used for Taqman qPCR or Sybr Green qPCR on a ViiA 7 real-time PCR system

(112) LncRNA SNHG10 increases the methylation of miR-218 gene to promote glucose uptake and cell proliferation in osteosarcomaJournal of Orthopaedic Surgery and ResearchDecember 1, 2020Pan He, Yongqiang Xu, Zhijun Wangclose to 2.0 were subjected to reverse transcriptions (RTs) using GoScript™ Reverse Transcriptase kit (A5003, Promega Corporation). With cDNA samples as template, qPCRs were performed using GeneRead qPCR SYBR

(113) SMOC2, an intestinal stem cell marker, is an independent prognostic marker associated with better survival in colorectal cancersScientific reportsSeptember 3, 2020Jang BG, Kim HS, Bae JM, Kim WH, Kim HU, Kang GH.oligo-dT primers and the GoScript reverse transcription system (Promega, Madison, WI, catalog number: A5004) to generate complementary DNA (cDNA). Subsequently, cDNA was used to perform real-time PCR with Premix

(114) SlGID1a Is a Putative Candidate Gene for qtph1.1, a Major-Effect Quantitative Trait Locus Controlling Tomato Plant HeightFrontiers in GeneticsAugust 7, 2020Xiaolin Liu et al.Analysis cDNA was synthesized from 2 μg total RNA using GoScriptTM Reverse Transcriptase (Cat. No. A5003; Promega, Madison, WI, United States). The qPCR reactions were conducted using the GoScriptTM qPCR Master Mix

(115) lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic NephropathyDiabetes, Metabolic Syndrome and Obesity: Targets and TherapyJuly 10, 2020Jinmei Zhang, Liwen Song, Yanjuan Ma, Yan Yin, Xinqi Liu, Xinyu Luo, Jiali Sun, Liqin WangDNAs. RNA integrity was checked using a Urea-PAGE gel (6%). RT-qPCR A reverse transcription system (A5003, Promega Corporation) was used to reverse transcribe RNA samples into cDNA samples. The qPCRs reactions

(116) lncRNA MEG8 Upregulates miR-770-5p Through Methylation and Promotes Cell Apoptosis in Diabetic NephropathyDiabetes Metab Syndr ObesJuly 10, 2020Jinmei Zhang, Liwen Song, Yanjuan Ma, Yan Yin, Xinqi Liu, Xinyu Luo, Jiali Sun, Liqin WangDNAs. RNA integrity was checked using a Urea-PAGE gel (6%). RT-qPCR A reverse transcription system (A5003, Promega Corporation) was used to reverse transcribe RNA samples into cDNA samples. The qPCRs reactions

(117) Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balanceAutophagyJuly 1, 2020Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo SJ, Lee JW, Moon C, Yu SW, Kim EK.MA, USA). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [50]. SYBR Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(118) Tentonin 3/TMEM150C senses blood pressure changes in the aortic archThe Journal of clinical investigationJuly 1, 2020Lu HJ, Nguyen TL, Hong GS, Pak S, Kim H, Kim H, Kim DY, Kim SY, Shen Y, Ryu PD, et al.Intron, catalog 17221) and reverse transcribed into cDNA using Reverse Transcriptase (GoScript, catalog A5003, Promega). Target genes were amplified by using DreamTaq DNA Polymerases (catalog EP0701, Thermo Fisher Scientific).

(119) Machine learning of reverse transcription signatures of variegated polymerases allows mapping and discrimination of methylated purines in limited transcriptomesNucleic Acids ResearchApril 17, 2020Stephan Werner et al.,New England Biolabs #2,AMV,AMV,M0277S,New England Biolabs #3,ProtoScript® II,MMLV,M0368S,New England Biolabs #4,GoScript™,MMLV,A5003,Promega #5,SuperScript™ III,MMLV,18080044,Thermo Fisher Scientific #6,RevertAid™,MMLV,EP0441,Thermo Fisher Scientific

(120) Trypsin activity governs increased susceptibility to pancreatitis in mice expressing human PRSS1R122HThe Journal of clinical investigationJanuary 2, 2020Gui F, Zhang Y, Wan J, Zhan X, Yao Y, Li Y, Haddock AN, Shi J, Guo J, Chen J, et al.(Qiagen). RNA (1 μg) was reverse transcribed to complementary DNA using the Go Script RT kit (catalog A5003; Promega). qPCR was done using PowerUp SYBR Green Master Mix (catalog A25742; Applied Biosystems). Primer

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