GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5003
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during AgingBiomedicinesMay 17, 2022Li T, Liu H, Jiang D, Yang K, Shen J, Feng H, Wang S, Zhang Y, Wang Y, Tang TS.reverse transcribed into complementary DNA using GoScript Reverse Transcription System (Promega, Cat#A5003, Fitchburg, Wisconsin, WI, USA). Quantitative real-time PCR was performed using SYBR Green QPCR Master

(82) TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancerNature communicationsApril 20, 2022Su Z, Monshaugen I, Wilson B, Wang F, Klungland A, Ougland R, Dutta A.DNase-treated total cellular RNA (refer to RNA isolation) by GoScript Reverse Transcriptase (Promega #A5004) with random primers (Invitrogen #48190011). RT-qPCR reactions were monitored on StepOnePlus Real-Time PCR System

(83) Interleukine-17 Modulates Neurogenesis and Behavior Following Exposure to Trauma in MiceCellsJanuary 20, 2022Willinger Y, Turgeman G.DNA (cDNA) was transcribed from 1.5 µg RNA using the GoScript™ Reverse Transcription System (cat. No. A5003, Promega, Madison, WI, USA) and was subjected to real-time PCR analysis using the AriaMx 96 RT-PCR System

(84) ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growthFrontiers in molecular biosciencesJanuary 1, 2022Park J, Park GY, Lee J, Park J, Kim S, Kim E, Park SY, Yoon JH, Lee Y.Thermo Fisher Scientific), and reverse transcription was performed using the GoScript™ Reverse Transcription System (A5004, Promega) according to the manufacturer’s instructions. SYBR Green real-time PCR master mix (TOQPK-201;

(85) CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in NeuroblastomaFrontiers in oncologyJanuary 1, 2022Qiu L, Zhou R, Luo Z, Wu J, Jiang H.Invitrogen, CA, USA), and conversion to cDNA was performed using the GoScript Reverse Transcription System (A5003, Promega, WI, USA). The mRNA expression levels of relevant target genes were quantified by real-time PCR

(86) Albendazole reduces hepatic inflammation and endoplasmic reticulum-stress in a mouse model of chronic Echinococcus multilocularis infectionPLoS neglected tropical diseasesJanuary 1, 2022Weingartner M, Stücheli S, Jebbawi F, Gottstein B, Beldi G, Lundström-Stadelmann B, Wang J, Odermatt A.kit and QIAcube were obtained from Qiagen (Venlo, Netherlands), GoScript reverse transcriptase (Cat# A5003) from Promega (Fitchburg, WI, USA), rabbit monoclonal anti-Lamin B1 antibody (Cat# ab133741, RRID:AB_2616597)

(87) Analysis of long and short enhancers in melanoma cell stateseLifeDecember 7, 2021David Mauduit, Ibrahim Ihsan Taskiran, Liesbeth Minnoye, Maxime de Waegeneer, Valerie Christiaens, Gert Hulselmans, Jonas Demeulemeester, Jasper Wouters, Stein Aertskit,Dynabeads mRNA purification kit,Ambion,Cat # 61006, Commercial assay or kit,GoScript RT kit,Promega,Cat # A5003, Recombinant DNA reagent,pGL4.23-GW,Promega,Addgene: 60323;RRID:Addgene_60323,Luciferase backbone vector Recombinant

(88) Modelling of primary ciliary dyskinesia using patient-derived airway organoidsEMBO reportsDecember 6, 2021van der Vaart J, Böttinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, et al.RNeasy Mini Kit according to protocol. For cDNA synthesis, GoScript Reverse Transcriptase (Promega; A5003) was used according to protocol. qPCRs were performed in 384‐well format using IQ SYBR Green (Bio‐Rad;

(89) Characterization and prognostic value of LXR splice variants in triple-negative breast canceriScienceOctober 22, 2021Lianto P, Hutchinson SA, Moore JB, Hughes TA, Thorne JL.& Nuclear RNA Purification Kit,Norgen,Cat. 21000 GoScriptTM Reverse Transcription kit,Promega,Cat. A5003 BCA kit,Thermo Fisher,Cat. 23227 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(90) HIV-1 Reverse Transcriptase Inhibition by Major Compounds in a Kenyan Multi-Herbal Composition (CareVid™): In Vitro and In Silico ContrastPharmaceuticals (Basel)September 30, 2021Winnie Rotich, Nicholas J. Sadgrove, Eduard Mas-Claret, Guillermo F. Padilla-González, Anastasia Guantai, Moses K. Langattranscriptase was purchased from Promega (Madison, WI, USA) (GoScript™ Reverse Transcriptase: catalogue number A5004) and the enzyme dilution buffer from Thermo Fisher (Waltham, MA, USA) (catalogue number B19). Briefly,

(91) Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infectionPhysiological ReportsSeptember 20, 2021Loretta Müller, Jakob Usemann, Marco P. Alves, Philipp LatzincDNA synthesis (using 200 ng of total RNA and the GoScript Reverse Transcription System; Promega, cat.no. A5003), quantitative real‐time RT‐PCR (384‐well plates, duplicates for target genes, quadruplicates for the

(92) Impact on Bile Acid Concentrations by Alveolar Echinococcosis and Treatment with Albendazole in MiceMetabolitesJuly 6, 2021Cristina Gómez, Fadi Jebbawi, Michael Weingartner, Junhua Wang, Simon Stücheli, Bruno Stieger, Bruno Gottstein, Guido Beldi, Britta Lundström-Stadelmann, Alex Odermatt1.5 and 2.0 were further processed. cDNA was synthesized using GoScript Reverse Transcriptase (Cat#A5003, Promega, Madison, WI, USA). The KAPA SYBR Fast Kit (Cat# SFUKB, Merck, Darmstadt, Germany) was used

(93) Human naive epiblast cells possess unrestricted lineage potentialCell stem cellJune 3, 2021Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al.using ReliaPrep kit (Promega, Z6012) and cDNA synthesized with GoScript reverse transcriptase (Promega, A5004) and oligo(dT) adaptor primers. TaqMan assays (Thermo Fisher Scientific) and Universal Probe Library

(94) A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damageCell reportsMay 11, 2021Gambino F Jr, Tai W, Voronin D, Zhang Y, Zhang X, Shi J, Wang X, Wang N, Du L, Qiao L.Kit,QIAGEN,27104 RNeasy Mini Prep Kit,QIAGEN,74104 GoScript Reverse Transcription System,Promega,A5003 iTaq Universal SYBRGreen Supermix,Bio-Rad,1725121 Experimental models: cell lines,Experimental models:

(95) DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunctioneLifeApril 26, 2021Lau EO, Damiani D, Chehade G, Ruiz-Reig N, Saade R, Jossin Y, Aittaleb M, Schakman O, Tajeddine N, Gailly P, et al.assay or kit,RNeasy Kit,Qiagen,Cat# 74004, Commercial assay or kit,RT cDNA synthesis Kit,Promega,Cat# A5003, Commercial assay or kit,SYBR Green SuperMix,Biorad,Cat# 170–8882, Commercial assay or kit,Lipofectamine

(96) Human alpha-synuclein overexpressing MBP29 mice mimic functional and structural hallmarks of the cerebellar subtype of multiple system atrophyActa neuropathologica communicationsApril 14, 2021Mészáros L, Riemenschneider MJ, Gassner H, Marxreiter F, von Hörsten S, Hoffmann A, Winkler J.concentration was measured using Nano-Drop technology (PeqLab). GoScript™ Reverse Transcription System (A5004, Promega) was applied for cDNA production. To quantify gene transcription, qPCRs were performed on a

(97) Palmitate reduces starvation-induced ER stress by inhibiting ER-phagy in hypothalamic cellsMolecular brainApril 6, 2021Lim Y, Kim S, Kim EK.Scientific). RNA (3 µg) was reverse transcribed using a GoScript Reverse Transcription System (Promega, A5004) as described previously [34]. TB Green (TaKaRa Biotechnology, RR820A) was used for quantitative PCR

(98) Expanding the genetic spectrum of primary familial brain calcification due to SLC2OA2 mutations: a case seriesneurogeneticsMarch 1, 2021Luca Magistrelli et al.recommendation. Total RNA was used to produce cDNA using the GoScript™ Reverse Transcriptase (Cat# A5003, Promega). SLC20A2 cDNA was amplified using the following primers: forward 5′-CCGTGTTACTAGGCGCCAAAGTAG-3′,

(99) LncRNA SLC16A1-AS1 is Upregulated in Glioblastoma and Promotes Cancer Cell Proliferation by Regulating miR-149 MethylationCancer Management and ResearchFebruary 10, 2021Yinbo Long, Heyang Li, Zhibin Jin, Xiang Zhangamong 1.8–2.0, which indicated the high quality of RNA. RT-qPCR Assay A Reverse Transcription System (A5003, Promega Corporation) was used to reverse transcribe RNA samples (from both in vitro cultivated cells

(100) Reverse Transcriptase: From Transcriptomics to Genome EditingTrends in BiotechnologyFebruary 1, 2021Samara Martín-Alonso, Estrella Frutos-Beltrán, Luis Menéndez-AriasRT) References (1) Promega (https://www.promega.es/products/pcr/rt‐pcr/goscript‐reverse‐ transcriptase/?catNum=A5003) (2) Bagnoli, J.W. et al. (2018). Sensitive and powerful single‐cell RNA sequencing using mcSCRB‐ seq.

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