GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5003
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(81) Antioxidant and Anti-Inflammatory Activities of Coenzyme-Q10 and Piperine against Cyclophosphamide-Induced Cytotoxicity in HuH-7 CellsBioMed Research InternationalJuly 13, 2022Norah S. AL-Johani, Mohammed Al-Zharani, Nada H. Aljarba, Norah M. Alhoshani, Nora Alkeraishan, Saad AlkahtaniUSA). 2.5.2. Complementary DNA (cDNA) Synthesis cDNA synthesis kit GoScript™ Reverse Transcriptase (CAT No A5003, Promega, USA) was carried out according to manufacturer instructions. Briefly, 0.5 μl Oligo (dT) and

(82) Gilteritinib Enhances Anti-Tumor Efficacy of CDK4/6 Inhibitor, Abemaciclib in Lung Cancer CellsFrontiers in PharmacologyJune 23, 2022Chao-Yue Sun, Milton Talukder, Di Cao, Cun-Wu Chenmanufacturer’s instruction. Then, cDNA was obtained using the GoScript Reverse Transcriptase kit (Promega, A5004). Real-time quantitative PCR (qRT-PCR) analysis was performed with GoTaq qPCR Master Mix kit (Promega,

(83) A single amino acid residue controls acyltransferase activity in a polyketide synthase from Toxoplasma gondiiiScienceJune 17, 2022Hannah K. D’Ambrosio, Jack G. Ganley, Aaron M. Keeler, Emily R. Derbyshireproteins" ,, ProSignal® Femto ECL Reagent,Prometheus,Cat # 20-302 GoScript Reverse Transcription System,Promega,Cat# A5003 Ni-NTA agarose,Qiagen,Cat# 30210 cOmplete Ultra EDTA-free,Sigma,Cat# 5892953001 Q5® High-Fidelity DNA Polymerase

(84) Rapid fluorescent vital imaging of olfactory epitheliumiScienceMay 20, 2022Nishijima H, Zunitch MJ, Yoshida M, Kondo K, Yamasoba T, Schwob JE, Holbrook EH.assays ,, Quick-RNA Miniprep,ZYMO RESEARCH,Cat# R1054 GoScript Reverse Transcriptase,Promega,Cat# A5003 2X SYBR Green qPCR Master Mix,Genesee Scientific,Cat# 42-116PG ,, Deposited data,Deposited data,Deposited

(85) Novel Loss-of-Function Variants in CHD2 Cause Childhood-Onset Epileptic Encephalopathy in Chinese PatientsGenes (Basel)May 19, 2022Xu Wang, Di Cui, Changhong Ding, Chunhong Chen, Xiaohui Wang, Fang Fang, Hong Jin, Xiaotun Renquantified, RNA was reversed transcribed to cDNA using the GoScript™ Reverse Transcriptase (Promega, A5003). PCR amplification and agarose gel electrophoresis were then performed for the splicing-site variant,

(86) BMP4 Exerts Anti-Neurogenic Effect via Inducing Id3 during AgingBiomedicinesMay 17, 2022Li T, Liu H, Jiang D, Yang K, Shen J, Feng H, Wang S, Zhang Y, Wang Y, Tang TS.reverse transcribed into complementary DNA using GoScript Reverse Transcription System (Promega, Cat#A5003, Fitchburg, Wisconsin, WI, USA). Quantitative real-time PCR was performed using SYBR Green QPCR Master

(87) TRMT6/61A-dependent base methylation of tRNA-derived fragments regulates gene-silencing activity and the unfolded protein response in bladder cancerNature communicationsApril 20, 2022Su Z, Monshaugen I, Wilson B, Wang F, Klungland A, Ougland R, Dutta A.DNase-treated total cellular RNA (refer to RNA isolation) by GoScript Reverse Transcriptase (Promega #A5004) with random primers (Invitrogen #48190011). RT-qPCR reactions were monitored on StepOnePlus Real-Time PCR System

(88) Interleukine-17 Modulates Neurogenesis and Behavior Following Exposure to Trauma in MiceCellsJanuary 20, 2022Willinger Y, Turgeman G.DNA (cDNA) was transcribed from 1.5 µg RNA using the GoScript™ Reverse Transcription System (cat. No. A5003, Promega, Madison, WI, USA) and was subjected to real-time PCR analysis using the AriaMx 96 RT-PCR System

(89) ERK phosphorylation disrupts the intramolecular interaction of capicua to promote cytoplasmic translocation of capicua and tumor growthFrontiers in molecular biosciencesJanuary 1, 2022Park J, Park GY, Lee J, Park J, Kim S, Kim E, Park SY, Yoon JH, Lee Y.Thermo Fisher Scientific), and reverse transcription was performed using the GoScript™ Reverse Transcription System (A5004, Promega) according to the manufacturer’s instructions. SYBR Green real-time PCR master mix (TOQPK-201;

(90) Albendazole reduces hepatic inflammation and endoplasmic reticulum-stress in a mouse model of chronic Echinococcus multilocularis infectionPLoS neglected tropical diseasesJanuary 1, 2022Weingartner M, Stücheli S, Jebbawi F, Gottstein B, Beldi G, Lundström-Stadelmann B, Wang J, Odermatt A.kit and QIAcube were obtained from Qiagen (Venlo, Netherlands), GoScript reverse transcriptase (Cat# A5003) from Promega (Fitchburg, WI, USA), rabbit monoclonal anti-Lamin B1 antibody (Cat# ab133741, RRID:AB_2616597)

(91) CDC27-ODC1 Axis Promotes Metastasis, Accelerates Ferroptosis and Predicts Poor Prognosis in NeuroblastomaFrontiers in oncologyJanuary 1, 2022Qiu L, Zhou R, Luo Z, Wu J, Jiang H.Invitrogen, CA, USA), and conversion to cDNA was performed using the GoScript Reverse Transcription System (A5003, Promega, WI, USA). The mRNA expression levels of relevant target genes were quantified by real-time PCR

(92) Analysis of long and short enhancers in melanoma cell stateseLifeDecember 7, 2021David Mauduit, Ibrahim Ihsan Taskiran, Liesbeth Minnoye, Maxime de Waegeneer, Valerie Christiaens, Gert Hulselmans, Jonas Demeulemeester, Jasper Wouters, Stein Aertskit,Dynabeads mRNA purification kit,Ambion,Cat # 61006, Commercial assay or kit,GoScript RT kit,Promega,Cat # A5003, Recombinant DNA reagent,pGL4.23-GW,Promega,Addgene: 60323;RRID:Addgene_60323,Luciferase backbone vector Recombinant

(93) Analysis of long and short enhancers in melanoma cell statesElifeDecember 7, 2021David Mauduit, Ibrahim Ihsan Taskiran, Liesbeth Minnoye, Maxime de Waegeneer, Valerie Christiaens, Gert Hulselmans, Jonas Demeulemeester, Jasper Wouters, Stein Aertskit,Dynabeads mRNA purification kit,Ambion,Cat # 61006, Commercial assay or kit,GoScript RT kit,Promega,Cat # A5003, Recombinant DNA reagent,pGL4.23-GW,Promega,Addgene: 60323;RRID:Addgene_60323,Luciferase backbone vector Recombinant

(94) Modelling of primary ciliary dyskinesia using patient-derived airway organoidsEMBO reportsDecember 6, 2021van der Vaart J, Böttinger L, Geurts MH, van de Wetering WJ, Knoops K, Sachs N, Begthel H, Korving J, Lopez-Iglesias C, Peters PJ, et al.RNeasy Mini Kit according to protocol. For cDNA synthesis, GoScript Reverse Transcriptase (Promega; A5003) was used according to protocol. qPCRs were performed in 384‐well format using IQ SYBR Green (Bio‐Rad;

(95) Characterization and prognostic value of LXR splice variants in triple-negative breast canceriScienceOctober 22, 2021Lianto P, Hutchinson SA, Moore JB, Hughes TA, Thorne JL.& Nuclear RNA Purification Kit,Norgen,Cat. 21000 GoScriptTM Reverse Transcription kit,Promega,Cat. A5003 BCA kit,Thermo Fisher,Cat. 23227 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models

(96) HIV-1 Reverse Transcriptase Inhibition by Major Compounds in a Kenyan Multi-Herbal Composition (CareVid™): In Vitro and In Silico ContrastPharmaceuticals (Basel)September 30, 2021Winnie Rotich, Nicholas J. Sadgrove, Eduard Mas-Claret, Guillermo F. Padilla-González, Anastasia Guantai, Moses K. Langattranscriptase was purchased from Promega (Madison, WI, USA) (GoScript™ Reverse Transcriptase: catalogue number A5004) and the enzyme dilution buffer from Thermo Fisher (Waltham, MA, USA) (catalogue number B19). Briefly,

(97) Diesel exposure increases susceptibility of primary human nasal epithelial cells to rhinovirus infectionPhysiological ReportsSeptember 20, 2021Loretta Müller, Jakob Usemann, Marco P. Alves, Philipp LatzincDNA synthesis (using 200 ng of total RNA and the GoScript Reverse Transcription System; Promega, cat.no. A5003), quantitative real‐time RT‐PCR (384‐well plates, duplicates for target genes, quadruplicates for the

(98) Impact on Bile Acid Concentrations by Alveolar Echinococcosis and Treatment with Albendazole in MiceMetabolitesJuly 6, 2021Cristina Gómez, Fadi Jebbawi, Michael Weingartner, Junhua Wang, Simon Stücheli, Bruno Stieger, Bruno Gottstein, Guido Beldi, Britta Lundström-Stadelmann, Alex Odermatt1.5 and 2.0 were further processed. cDNA was synthesized using GoScript Reverse Transcriptase (Cat#A5003, Promega, Madison, WI, USA). The KAPA SYBR Fast Kit (Cat# SFUKB, Merck, Darmstadt, Germany) was used

(99) Human naive epiblast cells possess unrestricted lineage potentialCell stem cellJune 3, 2021Guo G, Stirparo GG, Strawbridge SE, Spindlow D, Yang J, Clarke J, Dattani A, Yanagida A, Li MA, Myers S, et al.using ReliaPrep kit (Promega, Z6012) and cDNA synthesized with GoScript reverse transcriptase (Promega, A5004) and oligo(dT) adaptor primers. TaqMan assays (Thermo Fisher Scientific) and Universal Probe Library

(100) A vaccine inducing solely cytotoxic T lymphocytes fully prevents Zika virus infection and fetal damageCell reportsMay 11, 2021Gambino F Jr, Tai W, Voronin D, Zhang Y, Zhang X, Shi J, Wang X, Wang N, Du L, Qiao L.Kit,QIAGEN,27104 RNeasy Mini Prep Kit,QIAGEN,74104 GoScript Reverse Transcription System,Promega,A5003 iTaq Universal SYBRGreen Supermix,Bio-Rad,1725121 Experimental models: cell lines,Experimental models:

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