GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5003
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-GPLoS OneJuly 2, 2025Sarshti Kaushik, Sushila Maan, Kanisht Batra, Swati Sindhu, Vijay Kadian, Aman Kumardevelopment. Using GoScript™ cDNA kit (Promega). The cDNA was synthesized using GoScript™ cDNA kit (Promega, A5003). As per the manufacture’s protocol, 20 μl of reaction mixture was prepared by adding 1 μl of random

(22) Vimentin network dysregulation mediates neurite deficits in SNCA duplication Parkinson’s patient–derived midbrain neuronsSci AdvJune 6, 2025Yanni Schneider, Aron Koller, Alina Schweigert, Marie Andert, Laura Hoffmann, Sydney O’Brien, Lukas Seebauer, Sonja Plötz, Pavel Kielkowski, Jürgen Winkler, et al.Nano-Drop (PeqLab). Complementary DNA was generated using the GoScript Reverse Transcription System (A5004, Promega). For quantification of transcript levels, qPCR was performed on a LightCycler 480 (Roche)

(23) Reannotation of cancer mutations based on expressed RNA transcripts reveals functional non-coding mutations in melanomaThe American Journal of Human GeneticsJune 5, 2025Daniele Pepe, Xander Janssens, Kalina Timcheva, Grecia M. Marrón-Liñares, Benno Verbelen, Vasileios Konstantakos, Dylan De Groote, Jolien De Bie, Amber Verhasselt, Barbara Dewaele, et al.with GoScript Reverse Transcriptase, Random Primers, dNTP Mix, and RNasin Ribonuclease Inhibitors (#A5004, #C1181, #U1511, #N2511; Promega). RT-qPCR was performed on a QuantStudio 5 instrument (Thermo Fisher)

(24) Volumetric printed biomimetic scaffolds support in vitro lactation of human milk-derived mammary epithelial cellsSci AdvJune 4, 2025Amelia Hasenauer, Kajetana Bevc, Maxwell C. McCabe, Parth Chansoria, Anthony J. Saviola, Kirk C. Hansen, Karen L. Christman, Marcy Zenobi-WongOne hundred nanograms of RNA was retrotranscribed with a GoScript Reverse Transcriptase kit (Promega, A5003, Madison, Wisconsin, USA). cDNA was diluted 1:5 with ribonuclease-free water. RT-qPCR was performed

(25) Protocol for assessing the effect of diesel particulate matter on the liver in vivo through hydrodynamic tail vein injection in miceSTAR ProtocMay 3, 2025Young Uk Kim, Hyeon Ji Kim, Eunjeong Kimcommercial assays,Critical commercial assays ,, GoScript reverse transcription system,Promega,Cat# A5004 SYBR Green real-time PCR master mix,TOYOBO,QPK-201 ,, Experimental models: Organisms/strains,Experimental models

(26) Visual threat avoidance while host seeking by Aedes aegypti mosquitoesCell ReportsApril 22, 2025Geoff T. Meyerhof, Pratik Dhavan, Summer Blunk, Allison Bourd, Ramandeep Singh, Avinash Chandel, Craig Montellassays,Critical commercial assays In-Fusion cloning kit,Takara Biosciences,638909 cDNA synthesis kit,Promega,A5003 LightCycler 480 SYBR Green Master,Roche,04707516001 Deposited data,Deposited data,Deposited data Data,This

(27) Circular PVT1 promotes cardiac fibroblast activation interacting with miR-30a-5p and miR-125b-5pCell Death DisApril 21, 2025Alessia Bibi, Alisia Madè, Simona Greco, Jose Manuel Garcia-Manteiga, Anna Sofia Tascini, Spyros Tastsoglou, Germana Zaccagnini, Przemyslaw Leszek, Carlo Gaetano, Fabio MartelliDroplet Digital PCR System. Briefly, 25 ng of cDNA obtained with GoScript™ Reverse Transcriptase (Promega A5003), 1x QX200™ ddPCR™ EvaGreen Supermix (Bio-Rad 1864034), and 125 nM of both forward and reverse primers

(28) Lung B cells in ectopic germinal centers undergo affinity maturationProc Natl Acad Sci U S AApril 8, 2025Stephane M. Guillaume, William S. Foster, Isabel San Martín Molina, Emily M. Watson, Silvia Innocentin, Grant M. Kennedy, Alice E. Denton, Michelle A. LintermanSorter (BD Biosciences) into reverse transcription buffer containing GoScript Reverse Transcriptase (#A5004 Promega), RNase inhibitor (#EO0381 Thermo Fisher Scientific), DTT (#43816 Sigma), Random Hexamers (#SO142

(29) Tekt3 Safeguards Proper Functions and Morphology of Neuromast Hair BundlesInternational Journal of Molecular SciencesMarch 28, 2025Dongmei Su, Sirun Lu, Ling Zheng, Dong Liucomplementary DNA with the random primer using a complementary DNA synthesis kit (Promega, Madison, WI, USA, A5003). Quantitative real-time PCR primers for tekt gene members and gapdh are listed in Table S1. The real-time PCR

(30) Machine learning‑based construction of damage‑associated molecular patterns related score identifies subtypes of pancreatic adenocarcinoma with distinct prognosisOncology LettersMarch 24, 2025Jing Liang, Hui Wu, Zewen Song, Guoyin Li, Jianfeng Zhang, Wenxin DingScientific, Inc.). cDNA synthesis was performed according to the manufacturer's instructions (cat. no. A5003; GoScript™ Reverse Transcriptase from Promega Corp.). RT-qPCR was conducted as previously described

(31) Extracellular Vesicles from Regenerating Skeletal Muscle Mitigate Muscle Atrophy in an Amyotrophic Lateral Sclerosis Mouse ModelCellsMarch 20, 2025Jinghui Gao, Aria Sikal, Rachel Hankin, Yaochao Zheng, Elijah Sterling, Kenny Chan, Yao Yaointo complementary DNA (cDNA) using the GoScript Reverse Transcriptase kit (Promega, Madison, WI, USA, A5003). Quantitative real time-PCR (qRT-PCR) was performed to measure messenger RNA (mRNA) expression of IL-10

(32) Transcriptomic Profiling Reveals Differences in Slow‐Twitch and Fast‐Twitch Muscles of a Cigarette Smoke‐Exposed Rat ModelJournal of Cachexia, Sarcopenia and MuscleFebruary 1, 2025Timothy K. H. Fung, Kwok Kuen Cheung, Xia Wang, Benson W. M. Lau, Shirley P. C. Ngaifrom the extracted RNA using the GoScript™ Reverse Transcriptase Kit (Promega Corporation, USA, Cat. A5003). cDNA amplification was conducted using a CFX Connect Real‐Time PCR Detection System (Bio‐Rad Laboratories,

(33) YTHDF2 promotes ATP synthesis and immune evasion in B cell malignanciesCellJanuary 23, 2025Zhenhua Chen, Chengwu Zeng, Lu Yang, Yuan Che, Meiling Chen, Lillian Sau, Bintao Wang, Keren Zhou, Yu Chen, Ying Qing, et al.Scientific,A456-1 ZymoTaq PreMix,Zymo Research,Cat# E2004 GoScript Reverse Transcrptase,Promega,Cat# A5003 Recombinant YTHDF1,Active Motif,Cat# 31608 Recombinant YTHDF2,Active Motif,Cat# 31573 Recombinant

(34) Impacts of hnRNP A1 Splicing Inhibition on the Brain Remyelination ProteomeJ NeurochemJanuary 22, 2025Caroline Brandão-Teles, Victor Corasolla Carregari, Guilherme Reis-de-Oliveira, Bradley J. Smith, Yane Chaves, Aline Valéria Sousa Santos, Erick Martins de Carvalho Pinheiro, Caio C. Oliveira, Andre Schwambach Vieira, Fernanda Crunfli, et al.generate cDNA, 500 ng of RNA was reverse transcribed using the Promega reverse transcription kit (cat# A5003). For each 10 μL of the qPCR reaction, 1 μL of cDNA diluted 1:10 was mixed with 200 nM of the primers

(35) Inhibition of EETosis with an anti-citrullinated histone antibody: a novel therapeutic approach for eosinophilic inflammatory disordersFrontiers in immunologyJanuary 1, 2025Zwiers E, Montizaan D, Kip A, Waaijenberg K, Fichtinger PS, Mathur SK, Fujioka Y, Ueki S, van Es H, Chirivi RGS, et al.using RNeasy columns (Qiagen, 74106) and cDNA was made using GoScript reverse transcriptase (Promega, A5004). Equal volumes of cDNA from each sample were added to a solution of 1x SYBR green master mix (Applied Biosystems

(36) Releasing a sugar brake generates sweeter tomato without yield penaltyNatureNovember 21, 2024Jinzhe Zhang, Hongjun Lyu, Jie Chen, Xue Cao, Ran Du, Liang Ma, Nan Wang, Zhiguo Zhu, Jianglei Rao, Jie Wang, et al.Biotechnology), and the RNA was reverse transcribed, using GoScript Reverse Transcriptase (catalogue no. A5003; Promega), according to the manufacturer’s instructions. qPCR was performed using GoScript qPCR Master Mix

(37) Single-cell RNA sequencing analysis reveals the role of TXNDC5 in keloid formationCytojournalOctober 31, 2024Zhikun Liu, Lining Xian, Jianmin Li, Shudan Zheng, Hongju Xiewere collected and total RNA (1 mg) was extracted with the GoScript Reverse Transcription System kit (A5003, Promega Corporation, Madison, Wisconsin, USA). After quantification and synthesis of cDNA, quantitative

(38) NRXN1 as a Prognostic Biomarker: Linking Copy Number Variation to EMT and Survival in Colon CancerInternational journal of molecular sciencesOctober 24, 2024Bang HJ, Shim HJ, Park MR, Yoon S, Yoo KH, Kim YK, Lee H, Nam JS, Hwang JE, Bae WK, et al.reagent (Cat. No. 305–101; GeneAll Biotechnology, Seoul, Republic of Korea) and a GoScript kit (Cat. No. A5003; Promega, Madison, WI, USA), respectively, following the manufacturers’ protocols. RT-qPCR analysis

(39) TRAF7 determines circadian period through ubiquitination and degradation of DBPCommunications BiologyOctober 8, 2024Shusaku Masuda, Nobuhiro Kurabayashi, Rina Nunokawa, Yuta Otobe, Hiroko Kozuka-Hata, Masaaki Oyama, Yuri Shibata, Jun-ichiro Inoue, Michinori Koebis, Atsu Aiba, et al.manufacturer’s protocol. From purified RNA, cDNA was generated by Go Script Reverse Transcriptase (Promega, A5003) with both anchored (dT) 15 primer and random oligo primers or ReverTra Ace qPCR Master Mix (TOYOBO,

(40) Transcriptomic analysis of plasma-derived small extracellular vesicles reveals the pathological characteristics of normal tension glaucomaExtracellular Vesicles and Circulating Nucleic AcidsAugust 19, 2024Sheng-Lan Xu, Jun-Hua Li, Wen-Meng Zhang, Meng-Jun Fu, Hui-Min Xing, Hua Ma, Xian-Hui Gong, Rong-Han Wu, Yuan-Bo Liang, Ren-Zhe Cui, et al.Reverse transcription of mitochondrial RNA was performed using the GoScript Reverse transcription System (A5004, Promega, USA). Real-time PCR was performed using Taq pro Universal SYBR qPCR Master Mix (Q712-02, Vazyme,

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