GoScript Reverse Transcriptase from Promega

Product Specs
ItemGoScript Reverse Transcriptase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA5003
Quantity100 reactions

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(21) Unveiling the conserved mechanism of microsporidian vertical transmission: A comparative study of nosema infection across Host speciesVirulenceDecember 24, 2025Chunxia Wang, Yongzhi Kong, Zishen Tang, Tongyu Luo, Xuanang Yang, Yongjun Zhang, Tian Li, Zeyang ZhouThe cDNA was synthesized with 1 μg total RNA using the GoScript™ Reverse Transcription System Kit (A5003, Promega) after DNA digestion with DNase I. Relative ApVg mRNA levels were measured using qPCR with

(22) Regulation of vacuole fusion in stomata by dephosphorylation of the HOPS subunit VPS39Plant JDecember 19, 2025Anne-Marie Pullen, Grant Billings, Charles Hodgens, Gisele White, Belinda S. Akpa, Marcela Rojas-PierceReverse transcription was conducted using GoScript Reverse Transcriptase (Promega, Madison, WI, USA; A5003). qPCR was conducted using the Bio‐Rad CFX Connect real‐time PCR system and SYBR Green Master mix (Bio‐Rad,

(23) B-Cell Receptor-Associated Protein 31 Deficiency Aggravates Ethanol-Induced Liver Steatosis and Liver Injury via Attenuating Fatty Acid Oxidation and Glycogen SynthesisInt J Mol SciDecember 18, 2025Shubin Yu, Yaodong Xia, Chunyan Zhang, Xiangyue Han, Xiaoyue Feng, Liya Li, Hang Ma, Jialin XuSpectrophotometers and gel electrophoresis. Two micrograms of total RNA were converted to single-stranded cDNA (A5003, Promega Corporation; Madison, WI, USA). The relative mRNA levels were quantified using a CFX96 Touch™ Real-Time PCR Detection System

(24) Anti‐melanogenic effects of Medicago sativa seed extracts on melanocytesInternational Journal of Cosmetic ScienceDecember 1, 2025Chae Rin Kim, Jung Woo, Kyu Lim Kim, Minah Choi, Hee Jung Shin, Junoh Kim, Kyung Min Lim, Chang‐Seok Leeinstructions. cDNA was synthesized from total mRNA using the GoScript Reverse Transcription System (A5003; Promega, Madison, WI, USA) and a thermal cycler (BioRad, Hercules, CA, USA). Tyrosinase (qMmuCED0045245,

(25) Olfactory gene dynamics in invasive Indian and non-invasive African malaria vectors at the crossroads of development, infection and resistanceSci RepOctober 28, 2025Arvind Sharma, Bhuvan Dixit, Bharti Goyal, Renuka Harit, Jatin Kumar, Shibani Biswas, Ritu Goswami, Kailash C. Pandey, S. Noushin Emami, Soumyananda Chakraborticharacterized using quantitative RT-PCR (qRT-PCR). GoScript™ Reverse Transcriptase cDNA synthesis kit (Promega, A5003) was used to convert 1ug of total RNA to cDNA following manufacturer protocol. Briefly, cDNA from different

(26) COG6 is an essential host factor for influenza A virus infectionMicrobiology spectrumOctober 7, 2025Feng D, Guo J, Yan J, Chen J, Ding L, Zhu X, Chen Z, Hu Y, Zhang M, Liu J, et al.synthesized from total RNA isolated from WT A549 cells using GoScript Reverse Transcriptase (Promega, A5003). The coding sequence of COG6, fused with either a Flag or Myc tag, was cloned into pCAGGS vector for

(27) Biofloc system with synbiotic supplementation improves Penaeus vannamei immunity against AHPNDAppl Microbiol BiotechnolOctober 3, 2025Magdalena Lenny Situmorang, Anggie Nur Fadillah, Atian Azizy, Gede Suantikavisualization (Supplementary File). Next, the total RNA was converted into cDNA using the GoScript™ RT System (A5003, Promega) according to the manufacturer’s instructions. The cDNA samples obtained were then used as

(28) Glycans on non-structural protein 1 prevent premature T-cell mediated dengue virus clearanceEMBO Molecular MedicineSeptember 17, 2025Fakhriedzwan Idris, Justin Seng Geap Ooi, Donald Heng Rong Ting, Eunice Tze Xin Tan, Corrine Wan, Peter I Benke, Jan K Marzinek, Jack M Copping, Qin Hui Li, Lu Yi Ng, et al.kits,Molecular biology kits QIAamp Viral RNA,Qiagen,52904 GoScript™ Reverse Transcriptase,Promega,A5003 NEB Q5 Hot-Start high-fidelity 2× Master Mix,New England Biolabs,M0494S pCR™-Blunt II-TOPO™ Vector,Invitrogen,K280002 MinElute

(29) Establishing a pediatric solid tumor PDX biobank for precision oncology researchCancer Biol TherAugust 13, 2025Larissa Akemi Kido, Milena Rodrigues Marusco, Ellen Aparecida da Silva, Laís Do Carmo, Ana Beatriz Teodoro Borges, Felipe Luz Torres Silva, Juliana Silveira Ruas, Dieila Giomo de Lima, Larissa de Abreu Fernandes, Camila Maia Martin Daiggi, et al.synthesized following the GoScript Reverse Transcription System protocol (Promega™, Madison, WI, USA, Cat#A5003). The quality of synthesized cDNA was verified through control PCR for commonly expressed housekeeping

(30) Filamented Light (FLight) Bioprinting of Mini‐Muscles with Self‐Renewal PotentialAdvanced MaterialsJuly 23, 2025Hao Liu, Michael Winkelbauer, Jakub Janiak, Patrick Weber, Ali Kerem Kalkan, Inseon Kim, Parth Chansoria, Ori Bar‐Nur, Marcy Zenobi‐Wongtranscribed to complementary DNA following the instruction of GoScript Reverse Transcriptase kit (Promega, A5003). The relative gene expression levels were determined on real‐time PCR System (Applied Biosystems, QuantStudio

(31) High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminaseNature BiotechnologyJuly 7, 2025Yuan Wu, Yu-Lan Xiao, Weixin Tangcooled down on ice. Following primer annealing, 0.5 μL of the GoScript reverse transcriptase (Promega, A5003) was added together with 2 μL of 5× GoScript RT buffer, 1 μL of 25 mM MgCl2, 0.5 μL of 10 mM dNTPs,

(32) Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-GPLoS OneJuly 2, 2025Sarshti Kaushik, Sushila Maan, Kanisht Batra, Swati Sindhu, Vijay Kadian, Aman Kumardevelopment. Using GoScript™ cDNA kit (Promega). The cDNA was synthesized using GoScript™ cDNA kit (Promega, A5003). As per the manufacture’s protocol, 20 μl of reaction mixture was prepared by adding 1 μl of random

(33) Vimentin network dysregulation mediates neurite deficits in SNCA duplication Parkinson’s patient–derived midbrain neuronsSci AdvJune 6, 2025Yanni Schneider, Aron Koller, Alina Schweigert, Marie Andert, Laura Hoffmann, Sydney O’Brien, Lukas Seebauer, Sonja Plötz, Pavel Kielkowski, Jürgen Winkler, et al.Nano-Drop (PeqLab). Complementary DNA was generated using the GoScript Reverse Transcription System (A5004, Promega). For quantification of transcript levels, qPCR was performed on a LightCycler 480 (Roche)

(34) Reannotation of cancer mutations based on expressed RNA transcripts reveals functional non-coding mutations in melanomaThe American Journal of Human GeneticsJune 5, 2025Daniele Pepe, Xander Janssens, Kalina Timcheva, Grecia M. Marrón-Liñares, Benno Verbelen, Vasileios Konstantakos, Dylan De Groote, Jolien De Bie, Amber Verhasselt, Barbara Dewaele, et al.with GoScript Reverse Transcriptase, Random Primers, dNTP Mix, and RNasin Ribonuclease Inhibitors (#A5004, #C1181, #U1511, #N2511; Promega). RT-qPCR was performed on a QuantStudio 5 instrument (Thermo Fisher)

(35) Volumetric printed biomimetic scaffolds support in vitro lactation of human milk-derived mammary epithelial cellsSci AdvJune 4, 2025Amelia Hasenauer, Kajetana Bevc, Maxwell C. McCabe, Parth Chansoria, Anthony J. Saviola, Kirk C. Hansen, Karen L. Christman, Marcy Zenobi-WongOne hundred nanograms of RNA was retrotranscribed with a GoScript Reverse Transcriptase kit (Promega, A5003, Madison, Wisconsin, USA). cDNA was diluted 1:5 with ribonuclease-free water. RT-qPCR was performed

(36) Protocol for assessing the effect of diesel particulate matter on the liver in vivo through hydrodynamic tail vein injection in miceSTAR ProtocMay 3, 2025Young Uk Kim, Hyeon Ji Kim, Eunjeong Kimcommercial assays,Critical commercial assays ,, GoScript reverse transcription system,Promega,Cat# A5004 SYBR Green real-time PCR master mix,TOYOBO,QPK-201 ,, Experimental models: Organisms/strains,Experimental models

(37) Visual threat avoidance while host seeking by Aedes aegypti mosquitoesCell ReportsApril 22, 2025Geoff T. Meyerhof, Pratik Dhavan, Summer Blunk, Allison Bourd, Ramandeep Singh, Avinash Chandel, Craig Montellassays,Critical commercial assays In-Fusion cloning kit,Takara Biosciences,638909 cDNA synthesis kit,Promega,A5003 LightCycler 480 SYBR Green Master,Roche,04707516001 Deposited data,Deposited data,Deposited data Data,This

(38) Circular PVT1 promotes cardiac fibroblast activation interacting with miR-30a-5p and miR-125b-5pCell Death DisApril 21, 2025Alessia Bibi, Alisia Madè, Simona Greco, Jose Manuel Garcia-Manteiga, Anna Sofia Tascini, Spyros Tastsoglou, Germana Zaccagnini, Przemyslaw Leszek, Carlo Gaetano, Fabio MartelliDroplet Digital PCR System. Briefly, 25 ng of cDNA obtained with GoScript™ Reverse Transcriptase (Promega A5003), 1x QX200™ ddPCR™ EvaGreen Supermix (Bio-Rad 1864034), and 125 nM of both forward and reverse primers

(39) Lung B cells in ectopic germinal centers undergo affinity maturationProc Natl Acad Sci U S AApril 8, 2025Stephane M. Guillaume, William S. Foster, Isabel San Martín Molina, Emily M. Watson, Silvia Innocentin, Grant M. Kennedy, Alice E. Denton, Michelle A. LintermanSorter (BD Biosciences) into reverse transcription buffer containing GoScript Reverse Transcriptase (#A5004 Promega), RNase inhibitor (#EO0381 Thermo Fisher Scientific), DTT (#43816 Sigma), Random Hexamers (#SO142

(40) Tekt3 Safeguards Proper Functions and Morphology of Neuromast Hair BundlesInternational Journal of Molecular SciencesMarch 28, 2025Dongmei Su, Sirun Lu, Ling Zheng, Dong Liucomplementary DNA with the random primer using a complementary DNA synthesis kit (Promega, Madison, WI, USA, A5003). Quantitative real-time PCR primers for tekt gene members and gapdh are listed in Table S1. The real-time PCR

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