Alexander Raymour

Post-doctoral researcher

University of Maryland, College Park

Chemiluminescent Kit for Southern Blots
July 03, 2017
Our lab recently performed southern blots on DNA from a newly generated knockout mouse to ensure deletion of the gene of interest.... read more
Anti-CD28 Antibody For In Vitro Costimulation Of CD4+ T Cells
June 29, 2017
Our lab studies CD4+ T cells and often performs in vitro or ex vivo (re)activation using anti-CD3 + anti-CD28. read more
Alexa Fluor-Conjugated Secondary Antibody For Immunofluorescence
June 27, 2017
Our lab routinely performs IHC and IF on tissue sections. For IF, we use secondary antibodies from Invitrogen. They have a long ... read more
Blocking Buffer For LI-COR Western Blot
June 23, 2017
Our lab routinely uses the Li-COR Odyssey to visualize our western blots. There are a number of products that can be bought ... read more
OCT Compound for Frozen Tissue
June 21, 2017
Our lab routinely performs immunohistochemistry on both paraffin-embedded sections and frozen sections. We have found that some ... read more
Anti-CD3 Antibody For In Vitro Stimulation Of CD4+ T Cells
June 20, 2017
Our lab studies T cell function both in vitro and in vivo. For in vitro and ex vivo work, we use anti-CD3 + anti-CD28 ... read more
Western Blot Stripping Buffer
June 15, 2017
Western blots are routinely performed in our lab and often require probing with multiple primaries. This often requires us to ... read more
Synthesis Of Murine Myelin Oligodendrocyte Glycoprotein Peptide (35-55) For EAE
June 14, 2017
Our lab studies the role of CD4+ T cells in inflammatory processes both in vitro and in various in vivo models. One model we use ... read more
CD3 Antibody for Immunohistochemistry
June 13, 2017
Our lab studies the role of CD4+ T cells in inflammatory processes both in vitro and in various in vivo models. One model we use ... read more
Great Column-Based DNA Extraction Kit
June 12, 2017
DNA extraction is a standard practice in any molecular biology lab. This kit provides a quick and easy way to extract DNA from a ... read more
Anti-Mouse IgG2b Alexa Fluor 568 Secondary Antibody For IF And IHC
April 21, 2017
Our lab studies the role of CD4+ T cells in inflammatory processes both in vitro and in various in vivo models. One model we use ... read more
Anti-Mouse CD44 PE Antibody For Flow Cytometric Analysis Of Murine T Cells
April 18, 2017
One ongoing project going on in the lab is studying CD4+ T cells in in vivo mouse models. This requires a robust and reliable set ... read more
Magnetic Bead Isolation Of Mouse CD4+ T Cells
April 17, 2017
Our lab commonly isolates CD4+ T cells from mice to perform in vitro experiments. To isolate this cell population with high purity... read more
Compensation Beads For Flow Cytometry
April 14, 2017
Flow cytometry is a common technique in our lab which studies CD4+ T cells in inflammatory processes both in vitro and in vivo. To... read more
DETACHaBEAD Mouse CD4 Kit
April 13, 2017
Our lab analyzes murine CD4+ T cells and their role in inflammatory processes. Isolation of CD4+ T cells is often accomplished ... read more

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Effective and Easy-to-Prepare Reagent for Cell Permeabilization

Cell membrane permeabilization for intracellular staining and related cellular assays. I have used saponin as a permeabilization reagent when preparing cells ... read more

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