CD3 Antibody for Immunohistochemistry

June 13, 2017

BlueskyReddit
Cell Biology and Molecular Genetics
University of Maryland, College Park
Post-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dako

Product Name:

Polyclonal Anti-human CD3

Catalog Number:

A0452

Browse Similar Products: CD3 Immunohistochemistry Antibodies

Our lab studies the role of CD4+ T cells in inflammatory processes both in vitro and in various in vivo models. One model we use is experimental autoimmune encephalomyelitis (EAE). We often perform IHC on CNS and peripheral immune tissues to analyze various cell populations. The anti-CD3 antibody has worked great for us in mouse tissue for IHC.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse CNS tissue sections

Primary Incubation

Polyclonal anti-human CD3

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

Anti-goat IgG

Tertiary Incubation

DAB

Detection

Light microscopy

Results Summary

Staining works great on paraffin-embedded tissue. Clear staining can be seen with standard IHC protocols.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Great antibody for CD3 staining via IHC

The Bad

None

The Bottom Line

I would recommend this antibody for anyone doing IHC in mouse paraffin-embedded tissues.

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