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Author Profile
Andrea Estrada
Graduate Student
Colorado State University
Articles by Andrea Estrada
Assay Kit/Reagent User Review
This Bead Mix Is Not Compatible with Spectral Flow Cytometers
February 05, 2020
This product is not compatible with spectral flow cytometry for unmixing GFP.
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Assay Kit/Reagent User Review
Apogee Beads to Optimize on Analyzing EVs in Flow Cytometry
January 03, 2020
These beads are wonderful tools to use when first getting started with EV detection via flow cytometry! Optimizing them to detect...
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Equipment User Review
Detects Extracellular Vesicles!
December 30, 2019
I am really impressed with the ability of the Aurora to detect extracellular vesicles! The optimization has been easy and the ...
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Antibody User Review
Anti-Ubiquitin (Linkage Specific K-63) Antibody (For Western Blot)
March 02, 2018
This antibody was used to detect changes in K63 protein ubiquitination in response to various treatments. K63 linked ...
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Antibody User Review
Phospho-EIF2S1 Western Blot Antibody
February 26, 2018
This antibody was used to detect phosphorylation status of eIF2a, with the final outcome measurement being activation of the ...
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Antibody User Review
alpha-Tubulin Antibody for Western Blot
February 23, 2018
This antibody was selected to function as a loading control to normalize protein expression to. The specific application I use it...
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Antibody User Review
Anti-Ubiquitin (Linkage-Specific K48) Antibody
February 20, 2018
This antibody was used to detect polyubiquitin chains formed by Lysine-48 (K48) residue linkages via western blot. K48 ubiquitin ...
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Antibody User Review
beta-Actin Antibody for Western Blot
February 13, 2018
This antibody was purchased as a control to normalize protein expression to. It was selected because we multiplex this with ...
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Antibody User Review
EIF2-Alpha Antibody For Western Blot
February 12, 2018
This antibody was selected as a control to normalize the ratio of phosphorylated eIF2a to total eIF2-alpha. The context of this ...
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Antibody User Review
CHOP (DDIT3) Antibody
February 09, 2018
This antibody was used to detect the presence of endoplasmic reticulum stress / unfolded protein response activation, specifically...
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Antibody User Review
Pan Ubiquitin Antibody (For Western Blot)
February 05, 2018
This antibody was used to examine the effects of various nutrient treatments on protein ubiquitination in H4IIE whole cell lysates...
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Equipment User Review
Odyssey Clx For Western Blot Imaging
January 04, 2017
The Odyssey Clx is used for western blot imaging. The protocol is very similar to conventional western blotting. It calls ...
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Assay Kit/Reagent User Review
Streptavidin Coated Magnetic Beads
January 03, 2017
The general aim was to use streptavidin coated magnetic beads in order to capture biotin labeled protein. The goal was to purify ...
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Antibody User Review
ß-Actin: Good Antibody For Normalizing Protein To
December 14, 2016
Specific Aim: measure protein markers of endoplasmic reticulum stress in response to various stressors (chemical and environmental...
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Assay Kit/Reagent User Review
Biotin Labeling That Reacts With Sulfhydryl Groups
December 13, 2016
Research aim: measuring protein palmitoylation in response to various treatments. The goal was to capture palmitoylated proteins ...
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Product Review
Efficient and Cost Friendly SYBR Green Mastermix for qPCR
Parijat Sarkar
I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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