Cell Signaling Technology
beta-Actin
4970S
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Browse Similar Products: beta Actin Western Blot Antibodies
Specific Aim: measure protein markers of endoplasmic reticulum stress in response to various stressors (chemical and environmental).Chose this particular antibody because one of our ER stress markers produces a non-specific band right where alpha-tubulin shows up. Therefore, we needed another antibody to multiplex with that is at a lower molecular weight to normalize to.
Western Blot
Total lysates - H4IIE liver hepatoma cells
1:1000, 4C, overnight with gentle shaking
1:3 Odyssey buffer:TBS with 0.1% Tween20
1:15,000 goat-anti-rabbit (Licor IRDye800CW)
N/A
Odyssey Clx
Nice consistent band, showing up around 42KDa. Did not change with various cell treatments.
Great for normalizing protein levels to
No major drawbacks
Good control antibody, especially if you need multiplex!